Thermotank for cell culture

By introducing purification components and heat exchange tubes into the constant temperature box, the problem of the impact of low-oxygen gas pollutants is solved, air purification and temperature regulation are achieved, a stable low-oxygen constant temperature environment is provided, and the cell culture effect is improved.

CN223060989UActive Publication Date: 2025-07-04PEKING UNIVERSITY SHENZHEN HOSPITAL
View PDF 0 Cites 0 Cited by

Patent Information

Application Number
CN202421400870.3
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-06-19
Publication Date
2025-07-04
Estimated Expiration
2034-06-19

AI Technical Summary

Technical Problem

Existing low-oxygen constant temperature tanks are susceptible to pollutants when low-oxygen gases enter, resulting in poor cell culture effect.

Method used

A constant temperature chamber containing a purification assembly is designed, which includes a filter mesh and a filter element, which can filter impurities when gases enter, and heat exchange is combined with a heat exchange tube to adjust the temperature and provide a constant temperature and low oxygen environment.

Benefits of technology

Effectively filter impurities, ensure clean air, provide a stable low-oxygen constant temperature environment, and promote cell growth and culture effects.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN223060989U_ABST
    Figure CN223060989U_ABST
Patent Text Reader

Abstract

The utility model discloses a constant-temperature box for cell culture. The constant-temperature box comprises a bottom box, a heat exchange tube, a lower box, a lower edge strip, an upper box, an upper edge strip, a purification assembly, a culture assembly and a box cover, the heat exchange pipe is connected into the bottom box, and the two ends of the heat exchange pipe penetrate through one side of the bottom box. The upper box is located at the top of the lower box; the upper edge strip is connected to the bottom of the upper box and is connected with the lower edge strip through a plurality of bolts; the purification assembly is connected to one side of the bottom box; the culture assembly is connected inside the upper box and the lower box; the box cover is connected to the top of the upper box, and the top of the box cover communicates with an air suction pipe. Culture is conducted through the culture assemblies in the lower box and the upper box, all culture tools for culture are placed in the culture assemblies, the heat exchange pipes can conduct heat exchange with air when air enters the culture assemblies, and the purification assemblies suck low-oxygen gas to change the oxygen content, so that the air temperature and the oxygen content in the box body are changed, and a constant-temperature low-oxygen environment is created; different culture environments are simulated.
Need to check novelty before this filing date? Find Prior Art

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture, and particularly relates to an incubator for cell culture with constant temperature. Background Art

[0002] The hypoxic cell culture incubator is a device specially designed for cell culture. It can provide a constant temperature and a hypoxic environment to simulate the environment in vivo or under specific experimental conditions, so as to promote the growth, division, differentiation and reproduction of cells.

[0003] Immune cells such as liver cells and natural killer cells can be cultured in the hypoxic incubator. When performing internal and external ventilation, the external hypoxic gas directly enters. When there are more pollutant components in the hypoxic gas, it will affect cell culture. Therefore, there is an urgent need for a hypoxic cell culture incubator that can filter gas. Summary of the Utility Model

[0004] Based on the above technical problems, the utility model provides an incubator for cell culture.

[0005] Its specific technical solution is as follows:

[0006] An incubator for cell culture, comprising:

[0007] A bottom box;

[0008] A heat exchange tube, the heat exchange tube is connected inside the bottom box, and both ends of the heat exchange tube penetrate through one side of the bottom box;

[0009] A lower box, the lower box is connected to the top of the bottom box;

[0010] A lower side strip, the lower side strip is connected to the top of the lower box;

[0011] An upper box, the upper box is located on the top of the lower box;

[0012] An upper side strip, the upper side strip is connected to the bottom of the upper box, and the upper side strip and the lower side strip are connected by a plurality of bolts;

[0013] A purification component, the purification component is connected to one side of the bottom box;

[0014] A culture component, the culture component is connected to the inside of the upper box and the lower box;

[0015] A box cover, the box cover is connected to the top of the upper box, and an air suction pipe is communicated with the top of the box cover.

[0016] In the above technical solution, the purification component includes:

[0017] An installation port, the installation port is opened on one side of the bottom box;

[0018] Purification box, the purification box is installed in the installation port;

[0019] Filter screen, several of the filter screens are connected inside the purification box;

[0020] Filter element, the filter element is connected inside the filter screen;

[0021] Dust-proof net, the dust-proof net is connected to one side of the purification box;

[0022] Intake baffle, the intake baffle is connected to one side of the purification box.

[0023] In the above technical solution, the culture component includes:

[0024] Mounting plate, a pair of the mounting plates are respectively installed in the upper box and the lower box;

[0025] Mounting groove, two pairs of positioning columns are connected in the mounting groove;

[0026] Culture plate, a pair of positioning seats are connected to both sides of the culture plate, and the positioning columns are inserted into the positioning seats;

[0027] Placement holes, several of the placement holes are opened on the other side of the mounting plate, several connecting columns are movably connected around the placement holes, a clamping rod is connected to one side of the connecting column, and a torsion spring is connected between the connecting column and the mounting plate;

[0028] Blocking rods, several of the blocking rods are fixedly installed on the top of the mounting plate.

[0029] In the above technical solution, it further includes:

[0030] Through hole, the through hole is opened in the middle of the mounting plate;

[0031] Insertion plate, several insertion holes are opened at the top of the insertion plate, limiting blocks are connected to both ends of the insertion plate, and the insertion plate is located in the through hole.

[0032] In the above technical solution, it further includes:

[0033] Observation windows, several of the observation windows are respectively connected to the outer sides of the lower box, the upper box, the bottom box and the box cover.

[0034] In the above technical solution, it further includes:

[0035] Water pump, the water pump is connected to both ends of the heat exchange tube;

[0036] Water tank, the water tank is connected to the heat exchange tube.

[0037] A constant temperature incubator for cell culture of the present utility model, compared with the prior art, has the beneficial effects that:

[0038] Cultivation is carried out through the cultivation components inside the lower box and the upper box. All cultivation utensils for cultivation are placed inside the cultivation components. The purification component can filter the low-oxygen gas entering the bottom box during ventilation, filter out impurities in the purification component, ensure the cleanliness of the air inside the upper box, lower box, and bottom box. The heat exchange tube can conduct heat exchange with the air during air intake, thereby changing the gas temperature inside the box and adjusting a constant-temperature cultivation environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 Schematic diagram of a constant-temperature incubator for cell culture according to the present utility model;

[0040] Figure 2 Schematic diagram of the internal structure of the present utility model;

[0041] Figure 3 Schematic diagram of the internal structure of the present utility model;

[0042] Figure 4 Schematic diagram of the enlarged partial structure of the present utility model.

[0043] Wherein, Figures 1 to 4 The corresponding relationship between the reference numerals and the component names in

[0044] 1, bottom box; 2, heat exchange tube; 3, lower box; 4, lower side strip; 5, upper box; 6, upper side strip; 7, box cover; 8, installation opening; 9, purification box; 10, filter screen; 11, filter element; 12, dust-proof net; 13, intake baffle; 14, mounting plate; 15, installation groove; 16, culture plate; 17, placement hole; 18, retaining rod; 19, through hole; 20, plug board; 21, observation window; 22, water pump; 23, water tank. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0045] The following further illustrates the present utility model in conjunction with specific implementation cases and attached Figures 1-4 drawings, but the present utility model is not limited to these embodiments.

[0046] The present utility model discloses a constant-temperature incubator for cell culture, including: a bottom box 1, a heat exchange tube 2, a lower box 3, a lower side strip 4, an upper box 5, an upper side strip 6, a purification component, a cultivation component, and a box cover 7; the heat exchange tube 2 is connected inside the bottom box 1, and both ends of the heat exchange tube 2 penetrate through one side of the bottom box 1; the lower box 3 is connected to the top of the bottom box 1; the lower side strip 4 is connected to the top of the lower box 3; the upper box 5 is located on the top of the lower box 3; the upper side strip 6 is connected to the bottom of the upper box 5, and the upper side strip 6 and the lower side strip 4 are connected by a plurality of bolts; the purification component is connected to one side of the bottom box 1; the cultivation component is connected inside the upper box 5 and the lower box 3; the box cover 7 is connected to the top of the upper box 5, and an air suction pipe is communicated with the top of the box cover 7.

[0047] In the embodiments of the present utility model, asFigures 1-4 As shown in the figure, the purification component includes: an installation port 8, a purification box 9, a filter screen 10, a filter element 11, a dustproof net 12, and an intake baffle 13; the installation port 8 is opened on one side of the bottom box 1; the purification box 9 is installed in the installation port 8; several filter screens 10 are all connected inside the purification box 9; the filter element 11 is connected inside the filter screen 10; the dustproof net 12 is connected to one side of the purification box 9; the intake baffle 13 is connected to one side of the purification box 9.

[0048] In an embodiment of the present invention, as Figures 1-4 shown, the culture component includes: a mounting plate 14, a mounting groove 15, a culture plate 16, placing holes 17, and a retaining rod 18; a pair of mounting plates 14 are respectively installed in the upper box 5 and the lower box 3; two pairs of positioning columns are connected in the mounting groove 15; a pair of positioning seats are connected to both sides of the culture plate 16, and the positioning columns are inserted into the positioning seats; several placing holes 17 are all opened on the other side of the mounting plate 14, and several connecting columns are movably connected around the placing holes 17, a clamping rod is connected to one side of the connecting column, and a torsion spring is connected between the connecting column and the mounting plate 14; several retaining rods 18 are all fixedly installed on the top of the mounting plate 14.

[0049] In an embodiment of the present invention, as Figures 1-4 shown, it further includes: a through port 19 and an insertion plate 20; the through port 19 is opened in the middle of the mounting plate 14; several insertion holes are opened at the top of the insertion plate 20, limit blocks are connected to both ends of the insertion plate 20, and the insertion plate 20 is located in the through port 19.

[0050] In an embodiment of the present invention, as Figures 1-4 shown, it further includes: an observation window 21; several observation windows 21 are respectively connected to the outsides of the lower box 3, the upper box 5, the bottom box 1, and the box cover 7.

[0051] In an embodiment of the present invention, as Figures 1-4 shown, it further includes: a water pump 22 and a water tank 23; the water pump 22 is communicated with both ends of the heat exchange tube 2; the water tank 23 is connected to the heat exchange tube 2.

[0052] Implementation process: First, pour the temperature-control water, the temperature of which is self-regulated based on the temperature sensor inside the device, into the water tank 23. Then, start the water pump 22 to make the temperature-control water circulate in the heat exchange tube 2, and connect the air extraction device to the air suction pipe for air extraction. At this time, the low-oxygen gas enters from the air inlet baffle 13, passes through the dust-proof net 12, and then enters the filter screen 10 and the filter element 11, filtering impurities with different particle sizes in sequence. Finally, it enters the bottom box 1, carries the heat brought by the heat exchange tube 2 upward, and continues to flow upward through the jacks of the plug board 20. The plug board 20 and the jacks are used to temporarily fix various sensors. The bottom connecting rod bodies of various sensors are inserted into the jacks, and they can be used immediately, freely combined and matched. At the same time, the unused jacks can ventilate, which does not affect air circulation, and is sucked away from the top air suction pipe. While ventilating, it can both control the temperature and filter gas impurities.

[0053] In the description of the present utility model, the term "a plurality of" refers to two or more, unless otherwise clearly defined. The terms "upper", "lower", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation to the present utility model; the terms "connection", "installation", "fixation", etc. should all be understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0054] In the description of the present utility model, the descriptions of the terms "an embodiment", "some embodiments", "specific embodiments", etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In the present utility model, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0055] The above is only the preferred embodiment of the present utility model and is not used to limit the present utility model. For those skilled in the art, the present utility model can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model shall be included in the protection scope of the present utility model.

Claims

1. An incubator for cell culture, characterized in that, Comprising: Bottom box; Heat exchange tubes, which are connected inside the bottom box, and both ends of the heat exchange tubes penetrate through one side of the bottom box; Lower box, which is connected to the top of the bottom box; Lower side strip, which is connected to the top of the lower box; Upper box, which is located on the top of the lower box; Upper side strip, which is connected to the bottom of the upper box, and the upper side strip and the lower side strip are connected by a plurality of bolts; Purification component, which is connected to one side of the bottom box; Cultivation component, which is connected inside the upper box and the lower box; Box cover, which is connected to the top of the upper box, and an air suction pipe is communicated with the top of the box cover.

2. The incubator for cell culture according to claim 1, wherein, The purification component includes: Installation opening, which is opened on one side of the bottom box; Purification box, which is installed in the installation opening; Filter screens, a plurality of the filter screens are all connected inside the purification box; Filter element, which is connected inside the filter screen; Dust-proof net, which is connected to one side of the purification box; Air inlet baffle, which is connected to one side of the purification box.

3. The incubator for cell culture according to claim 1, characterized in that, The cultivation component includes: Installation plates, a pair of the installation plates are respectively installed inside the upper box and the lower box; Installation groove, and two pairs of positioning columns are connected inside the installation groove; Cultivation plate, a pair of positioning seats are connected to both sides of the cultivation plate, and the positioning columns are inserted into the positioning seats; Placement holes, a plurality of the placement holes are all opened on the other side of the installation plate, a plurality of connecting columns are movably connected around the placement holes, a clamping rod is connected to one side of the connecting column, and a torsion spring is connected between the connecting column and the installation plate; Blocking rods, a plurality of the blocking rods are all fixedly installed on the top of the installation plate.

4. The incubator for cell culture according to claim 3, wherein, It further includes: Through hole, which is opened in the middle of the installation plate; Insertion plate, a plurality of insertion holes are opened on the top of the insertion plate, limiting blocks are connected to both ends of the insertion plate, and the insertion plate is located inside the through hole.

5. The incubator for cell culture according to claim 1, characterized in that, It further includes: Observation windows, a plurality of the observation windows are respectively connected to the outsides of the lower box, the upper box, the bottom box and the box cover.

6. The incubator for cell culture according to claim 1, characterized in that, It further includes: Water pump, which is communicated with both ends of the heat exchange tubes; Water tank, which is connected to the heat exchange tubes.