Decoloring and dyeing device for coomassie brilliant blue rapid dye liquor

Abstract

The utility model discloses a decoloring and dyeing device for Coomassie brilliant blue rapid dye liquor, which relates to the technical field of dyeing and decoloring and comprises a shaking table and a shaking table, and a dyeing and decoloring component and a suction component are arranged around the shaking table; the dyeing and decoloring assembly comprises two raw material tanks, the two raw material tanks are communicated with a first pump through pipelines, and the discharge end of the first pump is fixedly connected with a feeding pipe; the suction assembly comprises a waste tank, the waste tank is communicated with a second pump through a pipeline, the function of integrating and simplifying the decolorizing and dyeing steps of the Coomassie brilliant blue rapid dye liquor is achieved, the operation steps are simplified, time is saved, gel does not need to be taken back and forth in the whole process, the possibility that the gel is polluted is reduced, and the production efficiency is improved. And the working vessel and the cradle can be connected and fixed, so that the stability of the working process is improved, and the problems that the existing Coomassie brilliant blue rapid dye liquor has more decolorizing and dyeing operation steps and is longer in time are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of dyeing and decolorization, in particular to a decolorization and dyeing device for Coomassie brilliant blue rapid dye solution. Background Technique

[0002] Coomassie blue rapid dye is a ready-to-use dye solution for protein gel staining. It can stain quickly, sensitively and safely. In the laboratory, this dye is widely used in protein analysis and identification, as well as protein detection in the production and quality control of biological drugs;

[0003] When the existing Coomassie brilliant blue rapid dye solution is used, the gel after electrophoresis needs to be cleaned with double-distilled water or deionized water first. After cleaning, the dye solution is added for staining, and then water is added for decolorization. These steps are all carried out separately, which greatly increases the dyeing and decolorization time. And during the processes of cleaning, staining and decolorization, the experimenter needs to constantly pick up the working dish and operate on the gel in the working dish to remove water and dye. Frequent picking up of the gel may cause the gel to be contaminated, resulting in errors. Content of the Utility Model

[0004] The main purpose of the utility model is to provide a decolorization and dyeing device for Coomassie brilliant blue rapid dye solution, which can effectively solve the technical problems in the background technique.

[0005] To achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0006] A decolorization and dyeing device for Coomassie brilliant blue rapid dye solution, including a shaker and a shaking table. A dyeing and decolorization component and a suction component are arranged around the shaker;

[0007] The dyeing and decolorization component includes two raw material tanks. Both of the two raw material tanks are connected to a pump I through pipelines. The discharge end of the pump I is fixedly connected with a feeding pipe;

[0008] The suction component includes a waste tank. The waste tank is connected to a pump II through a pipeline. The suction end of the pump II is fixedly connected with a extraction pipe.

[0009] As a further scheme of the utility model, the bottom end of the feeding pipe is fixedly connected with a spray head, and a certain angle is arranged between the spray head and the feeding pipe.

[0010] As a further scheme of the utility model, the extraction pipe is in a "person" - shaped structure, and both bottom ends of the extraction pipe are fixedly connected with suction heads, and the suction heads are in a structure with a smaller upper part and a larger lower part.

[0011] As a further scheme of the utility model, a fixing structure for fixing the working dish is arranged on the shaking table;

[0012] The fixed structure includes a bidirectional lead screw, and the surfaces of the bidirectional lead screw are respectively threadedly connected with two threaded tubes. One end of each of the two threaded tubes facing away from each other is respectively rotatably installed with a first clamping plate and a second clamping plate.

[0013] As a further solution of the present utility model, one end of the first clamping plate away from the threaded tube is connected to the feeding tube or the extraction tube through a buckle.

[0014] As a further solution of the present utility model, an adjusting ring is fixedly arranged at the central position of the bidirectional lead screw.

[0015] The beneficial effects of the present utility model are as follows:

[0016] By setting the dyeing and decolorizing assembly and the sucking assembly, multiple steps of decolorizing and dyeing the Coomassie brilliant blue rapid staining solution are integrated, greatly reducing the operation time, simplifying the operation steps, and not requiring any operation on the gel throughout the process, reducing the possibility of contaminating the gel, and effectively improving the convenience and stability of decolorizing and dyeing the Coomassie brilliant blue rapid staining solution;

[0017] By setting the fixed structure, the feeding tube and the extraction tube can be fixed in working dishes with different shapes and sizes, and while adjusting the positions of the nozzle and the suction head, the working dish can be fixed in the shaking table, making the entire dyeing and decolorizing process more stable and reliable. Description of the Drawings

[0018] Figure 1 It is a schematic diagram of the overall structure of a device for decolorizing and dyeing Coomassie brilliant blue rapid staining solution according to the present utility model;

[0019] Figure 2 It is a schematic diagram of the dyeing and decolorizing assembly and the sucking assembly of a device for decolorizing and dyeing Coomassie brilliant blue rapid staining solution according to the present utility model;

[0020] Figure 3 It is a schematic diagram of the split fixed structure of a device for decolorizing and dyeing Coomassie brilliant blue rapid staining solution according to the present utility model;

[0021] Figure 4 It is a schematic diagram of a device for decolorizing and dyeing Coomassie brilliant blue rapid staining solution according to the present utility model Figure 2 and the enlarged view of area A therein;

[0022] Figure 5 It is a schematic diagram of a device for decolorizing and dyeing Coomassie brilliant blue rapid staining solution according to the present utility model Figure 2 and the enlarged view of area B therein.

[0023] In the figure: 1, shaker; 2, rocking table; 3, dyeing and decolorizing assembly; 4, suction assembly; 5, fixing structure; 301, raw material tank; 302, pump 1; 303, feeding pipe; 304, spray head; 401, waste tank; 402, pump 2; 403, extraction pipe; 404, suction head; 501, clamping plate 1; 502, buckle; 503, threaded pipe; 504, bidirectional lead screw; 505, adjusting ring; 506, clamping plate 2. Specific embodiments

[0024] In order to make the technical means, creative features, achieved purposes and effects realized by the present utility model easy to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0025] As Figures 1-5 shown, a decolorizing and dyeing device for Coomassie brilliant blue rapid dye solution includes a shaker 1 and a rocking table 2. A dyeing and decolorizing assembly 3 and a suction assembly 4 are arranged around the shaker 1. The dyeing and decolorizing assembly 3 includes two raw material tanks 301, and Coomassie brilliant blue rapid dye solution and double-distilled water are respectively contained in the two raw material tanks 301 for cleaning, dyeing and decolorizing the gel. The raw material tank 301 is connected to a pump 1 302 through a pipeline, and the discharge end of the pump 1 302 is fixedly connected to a feeding pipe 303. The suction assembly 4 includes a waste tank 401, the waste tank 401 is connected to a pump 2 402 through a pipeline, and the suction end of the pump 2 402 is fixedly connected to an extraction pipe 403.

[0026] In this embodiment, the bottom end of the feeding pipe 303 is fixedly connected to a spray head 304, and a certain angle is provided between the spray head 304 and the feeding pipe 303. The angle set by the spray head 304 prevents liquid droplets from splashing when adding Coomassie brilliant blue rapid dye solution or double-distilled water, enhancing the stability of raw material addition.

[0027] In this embodiment, the extraction pipe 403 is in a "person" - shaped structure, and suction heads 404 are fixedly connected to both bottom ends of the extraction pipe 403. The suction head 404 has a structure with a smaller upper part and a larger lower part, preferably a frustum structure or a trumpet - shaped structure, etc. The structure of the suction head 404 with a smaller upper part and a larger lower part can increase the contact area between the suction head 404 and the liquid in the working dish, and drain the liquid in the culture dish more quickly.

[0028] In this embodiment, a fixing structure 5 is provided between the turntable 2 and the working dish. The fixing structure 5 includes a bidirectional lead screw 504. There are two threaded tubes 503 threadedly connected to the surface of the bidirectional lead screw 504. By rotating the bidirectional lead screw 504, the total length of the threaded tubes 503 and the bidirectional lead screw 504 can be quickly adjusted. One end of each of the two threaded tubes 503 away from the bidirectional lead screw 504 is rotatably installed with a first clamping plate 501 and a second clamping plate 506 respectively. There are four first clamping plates 501, and the first clamping plates 501 clamp the working dish, and the second clamping plate 506 clamps the turntable 2, thus playing a role in stabilizing the working dish, and cooperating with the bidirectional lead screw 504, working dishes of different shapes and sizes can be fixed, enhancing the stability during the dyeing and decoloring operations.

[0029] In this embodiment, one end of the first clamping plate 501 away from the threaded tube 503 is connected to the feeding tube 303 or the extraction tube 403 through a buckle 502. The first clamping plate 501 and the buckle 502 play a role in fixing the position of the feeding tube 303 or the extraction tube 403, and the fixing position of the feeding tube 303 or the extraction tube 403 can be adjusted by opening and closing the buckle 502, so that the feeding and extraction of the solution are faster and more stable.

[0030] In this embodiment, an adjusting ring 505 is fixedly arranged at the central position of the bidirectional lead screw 504. The adjusting ring 505 facilitates manually rotating the bidirectional lead screw 504, so as to facilitate adjusting the distance between the first clamping plate 501 and the second clamping plate 506.

[0031] It should be noted that the present utility model is a decoloring and dyeing device for Coomassie brilliant blue rapid dye solution. When in use, first add Coomassie brilliant blue rapid dye solution and double-distilled water into the two raw material tanks 301 respectively, then place the electrophoresed gel into the working dish. The feeding tube 303 and the extraction tube 403 are fixed inside the working dish through the first clamping plate 501. The positions of the nozzle 304 and the suction head 404 can be adjusted through the buckle 502. By rotating the adjusting ring 505 to drive the bidirectional lead screw 504 to rotate, the positions of the two threaded tubes 503 are adjusted, so that the second clamping plate 506 can clamp the turntable 2, and thus the working dish can be fixed. Start the first pump 302 to add double-distilled water into the working dish through the feeding tube 303 and the nozzle 304 to wash the gel. Start the shaker 1 to enhance the washing speed and effect. After the washing is completed, turn off the first pump 302. Start the second pump 402 to extract the liquid into the waste tank 401 through the suction head 404 and the extraction tube 403. After the extraction is completed, start the first pump 302 to add Coomassie brilliant blue rapid dye solution for dyeing. The solution after dyeing can be discharged into the waste tank 401, and then double-distilled water is pumped in for decoloring. After decoloring, the solution is discharged into the waste tank 401. The whole steps of dyeing and decoloring are concentrated together, simplifying the steps of manual operation, and the gel does not need to be taken multiple times during the dyeing and decoloring process.

[0032] The above has shown and described the basic principles, main features and advantages of the present utility model. Those skilled in the art should understand that the present utility model is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principles of the present utility model. Without departing from the spirit and scope of the present utility model, the present utility model will have various changes and improvements, and these changes and improvements all fall within the scope of the present utility model claimed. The scope of protection claimed by the present utility model is defined by the appended claims and their equivalents.

Claims

1. A decolorization and staining device for Coomassie brilliant blue rapid staining solution, comprising a shaker (1) and a shaking table (2), characterized in that: A dyeing and decolorizing assembly (3) and a suction assembly (4) are arranged around the shaker (1); The dyeing and decolorizing assembly (3) includes two raw material tanks (301). Both of the two raw material tanks (301) are connected to a first pump (302) through pipelines. The discharge end of the first pump (302) is fixedly connected to a feeding pipe (303); The suction assembly (4) includes a waste tank (401). The waste tank (401) is connected to a second pump (402) through a pipeline. The suction end of the second pump (402) is fixedly connected to a extraction pipe (403).

2. A decolorizing and staining device for Coomassie Brilliant Blue rapid staining solution according to claim 1, characterized in that: The bottom end of the feeding pipe (303) is fixedly connected to a spray head (304). A certain angle is provided between the spray head (304) and the feeding pipe (303).

3. A decolorizing and staining device for Coomassie Brilliant Blue rapid staining solution according to claim 1, characterized in that: The extraction pipe (403) is in a "person" - shaped structure, and both bottom ends of the extraction pipe (403) are fixedly connected to suction heads (404). The suction heads (404) are in a structure with a smaller upper part and a larger lower part.

4. A decolorization and staining device for Coomassie brilliant blue rapid staining solution according to claim 1, characterized in that: A fixing structure (5) for fixing the working dish is arranged on the shaking table (2); The fixing structure (5) includes a bidirectional lead screw (504). Two threaded pipes (503) are respectively thread - connected to the surface of the bidirectional lead screw (504). One end of each of the two threaded pipes (503) facing away from each other is respectively rotatably installed with a first clamping plate (501) and a second clamping plate (506).

5. A decolorizing and staining device for Coomassie Brilliant Blue rapid staining solution according to claim 4, characterized in that: One end of the first clamping plate (501) away from the threaded pipe (503) is connected to the feeding pipe (303) or the extraction pipe (403) through a buckle (502).

6. A decolorization and staining device for Coomassie Brilliant Blue rapid staining solution according to claim 4, characterized in that: An adjusting ring (505) is fixedly arranged at the central position of the bidirectional lead screw (504).

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