Multifunctional cell culture bag and cell culture equipment

Abstract

The utility model discloses a multifunctional cell culture bag and cell culture equipment, and belongs to the field of cell culture devices. The multifunctional cell culture bag comprises a bag body, a liquid adding pipe and a first liquid stopping piece. The bag body is provided with a containing cavity, and one end of the containing cavity is provided with a first connecting pipe communicating with the containing cavity. The liquid adding pipe comprises a first pipe body, a first connecting part and a second connecting part, the first pipe body is connected with the first connecting part and the second connecting part, the first connecting part is in threaded connection with the first connecting pipe, and the second connecting part is used for being in threaded connection with a bottle opening of the liquid storage bottle; the first liquid stopping piece is arranged on the first pipe body in a sleeving mode and located at the end, close to the first connecting part, of the first pipe body. The multifunctional cell culture bag provided by the utility model is simple and convenient to operate, time-saving and labor-saving, and the risk that cell sap in the accommodating cavity is polluted is effectively reduced.

Description

Technical Field

[0001] The present application relates to the technical field of cell culture devices, and particularly to a multifunctional cell culture bag and a cell culture device. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce, and maintain their main structures and functions. Currently, cell culture bags are generally used for culturing a large number of cells.

[0003] In the related art, a cell culture bag usually includes a bag body and a tube cap. One end of the bag body is provided with a connecting tube communicating with the accommodating cavity of the bag body, and the tube cap is covered on the connecting tube and is threadedly connected to the connecting tube. When adding liquid to the accommodating cavity during the culture process, the operator needs to unscrew the tube cap, and then inject the liquid into the accommodating cavity through the connecting tube with a syringe barrel without a needle. Since the volume of the liquid to be added is much larger than the volume of the syringe barrel, multiple extractions and multiple injections are required, resulting in cumbersome operations and time-consuming and laborious work. Summary of the Utility Model

[0004] In view of this, the purpose of the present application is to overcome the deficiencies in the prior art and provide a multifunctional cell culture bag to solve the technical problem that in the prior art, due to the volume of the liquid to be added being much larger than the volume of the syringe barrel, multiple extractions and multiple injections are required, resulting in cumbersome operations and time-consuming and laborious work.

[0005] To solve the above technical problem, the present application provides:

[0006] A multifunctional cell culture bag, comprising:

[0007] A bag body having an accommodating cavity, and one end of the accommodating cavity has a first connecting tube communicating with the accommodating cavity;

[0008] A liquid adding tube, the liquid adding tube includes a first tube body, a first connecting portion, and a second connecting portion. The first tube body is respectively connected to the first connecting portion and the second connecting portion. The first connecting portion is threadedly connected to the first connecting tube, and the second connecting portion is used for threadedly connecting to the mouth of a liquid storage bottle;

[0009] A first liquid stopping member, sleeved on the first tube body and located at one end of the first tube body close to the first connecting portion.

[0010] In addition, the multifunctional cell culture bag according to the present application may further have the following additional technical features:

[0011] In some embodiments of the present application, the multi-functional cell culture bag further includes a second liquid stop member and a controller, the controller is electrically connected to the second liquid stop member, the second liquid stop member is sleeved on the first tube body, and is located at one end of the first tube body close to the second connection portion.

[0012] In some embodiments of the present application, the controller includes a time adjustment interface for adjusting the relaxation time of the second liquid stop member, and the time adjustment interface is configured with a plurality of time gears for adjusting time.

[0013] In some embodiments of the present application, the multi-functional cell culture bag further includes a buffer container, the buffer container is connected to the first tube body, and is located between the first liquid stop member and the second liquid stop member.

[0014] In some embodiments of the present application, the multi-functional cell culture bag further includes a sampling member. One end of the accommodating cavity close to the first connecting tube has a second connecting tube communicating with the accommodating cavity. The sampling member includes an elastic bladder and a third connecting portion. One end of the third connecting portion is connected to the elastic bladder, and the other end is threadedly connected to the second connecting tube.

[0015] In some embodiments of the present application, a volume scale mark is provided on the outer surface of the elastic bladder.

[0016] In some embodiments of the present application, the sampling member further includes a fourth connecting portion. One end of the fourth connecting portion is connected to the elastic bladder, and the other end is used for connecting to the head of a sampling syringe barrel.

[0017] In some embodiments of the present application, the multi-functional cell culture bag further includes a liquid extraction tube. One end of the accommodating cavity close to the first connecting tube has a third connecting tube communicating with the accommodating cavity. The liquid extraction tube includes a second tube body, a fifth connecting portion and a sixth connecting portion. The second tube body is respectively connected to the fifth connecting portion and the sixth connecting portion. The fifth connecting portion is threadedly connected to the third connecting tube, and the sixth connecting portion is used for threadedly connecting to the mouth of a centrifuge bottle.

[0018] In some embodiments of the present application, the multi-functional cell culture bag further includes a fixing member. The fixing member is provided on one side of the bag body. The fixing member is provided with a first card slot adapted to the first tube body and a second card slot adapted to the second tube body.

[0019] In a second aspect, an embodiment of the present application further provides a cell culture device, including the multi-functional cell culture bag according to any one of the above embodiments.

[0020] Compared with the prior art, the beneficial effects of the present application are:

[0021] The present application provides a multi-functional cell culture bag. By threadedly connecting the first connecting part with the first connecting tube and using the second connecting part to threadedly connect with the bottle mouth of the liquid storage bottle, the liquid storage bottle is communicated with the accommodating cavity of the bag body through the liquid adding tube. When liquid needs to be added to the accommodating cavity during the culture process, the operator only needs to lift and invert the liquid storage bottle so that the liquid in the liquid storage bottle can be automatically added to the accommodating cavity of the bag body successively through the bottle mouth of the liquid storage bottle, the liquid adding tube and the first connecting tube under the action of gravity. The operation is simple and convenient, saving time and effort. It avoids the technical problems in the prior art that since the volume of the liquid to be added is much larger than the volume of the syringe barrel, multiple extractions and multiple injections are required, resulting in cumbersome operation and time-consuming and laborious work.

[0022] By sleeving a first liquid stop member on the first tube body at one end of the first tube body close to the first connecting part, when liquid needs to be added to the accommodating cavity, the operator first manually opens the first liquid stop member to open the first tube body, and then lifts and inverts the liquid storage bottle so that the liquid in the liquid storage bottle can be automatically added to the accommodating cavity of the bag body successively through the bottle mouth of the liquid storage bottle, the liquid adding tube and the first connecting tube under the action of gravity. After the liquid adding operation is completed, the operator manually closes the first liquid stop member to close the first tube body, playing a role of sealing and isolation, effectively reducing the risk of contamination of the cell liquid in the accommodating cavity. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present application and should not be regarded as limiting the scope. For those of ordinary skill in the art, other related drawings can be obtained based on these drawings without creative efforts.

[0024] Figure 1 Shows a cross-sectional schematic view of the multi-functional cell culture bag in some embodiments of the present application Figure 1 ;

[0025] Figure 2 Shows a cross-sectional schematic view of the multi-functional cell culture bag in some embodiments of the present application Figure 2 ;

[0026] Figure 3 Shows a front view schematic diagram of the multi-functional cell culture bag in some embodiments of the present application.

[0027] MAIN ELEMENT SYMBOL DESCRIPTION:

[0028] 100 - Multifunctional cell culture bag; 110 - Bag body; 111 - Accommodating cavity; 112 - First connecting pipe; 113 - Second connecting pipe; 114 - Third connecting pipe; 120 - Liquid adding pipe; 121 - First pipe body; 122 - First connecting part; 123 - Second connecting part; 130 - First liquid stop member; 140 - Second liquid stop member; 150 - Buffer container; 160 - Sampling member; 161 - Elastic bladder; 162 - Third connecting part; 163 - Fourth connecting part; 170 - Liquid extraction pipe; 171 - Second pipe body; 172 - Fifth connecting part; 173 - Sixth connecting part; 180 - Fixing member; 181 - First card slot; 182 - Second card slot. Detailed implementation manners

[0029] The embodiments of the present application will be described in detail below. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals denote the same or similar elements or elements with the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present application and should not be construed as a limitation to the present application.

[0030] In the description of the present application, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application.

[0031] In addition, the terms "first" and "second" are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present application, "a plurality of" means two or more unless otherwise specifically defined.

[0032] In the present application, unless otherwise clearly specified and defined, the terms "installed", "connected", "connected", "fixed", etc. should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0033] In this application, unless otherwise clearly specified and defined, the first feature being "on" or "under" the second feature may be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may be that the first feature is directly above or obliquely above the second feature, or merely indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "underneath" the second feature may be that the first feature is directly below or obliquely below the second feature, or merely indicates that the first feature has a lower horizontal height than the second feature.

[0034] Example 1

[0035] As Figure 1 shown, an embodiment of the present application provides a multifunctional cell culture bag 100. The multifunctional cell culture bag 100 includes a bag body 110, a liquid adding tube 120 and a first liquid stopping member 130.

[0036] Wherein, the bag body 110 has a containing cavity 111, and one end of the containing cavity 111 has a first connecting tube 112 communicating with the containing cavity 111. The liquid adding tube 120 includes a first tube body 121, a first connecting portion 122 and a second connecting portion 123. The first tube body 121 is respectively connected to the first connecting portion 122 and the second connecting portion 123. The first connecting portion 122 is threadedly connected to the first connecting tube 112, and the second connecting portion 123 is used for threadedly connecting with the bottle mouth of a liquid storage bottle. The first liquid stopping member 130 is sleeved on the first tube body 121 and is located at one end of the first tube body 121 close to the first connecting portion 122.

[0037] For the multifunctional cell culture bag 100 provided by the embodiment of the present application, the bag body 110 has a containing cavity 111, one end of the containing cavity 111 has a first connecting tube 112 communicating with the containing cavity 111, and the liquid adding tube 120 includes a first tube body 121, a first connecting portion 122 and a second connecting portion 123. The first tube body 121 is respectively connected to the first connecting portion 122 and the second connecting portion 123. By threadedly connecting the first connecting portion 122 to the first connecting tube 112 and using the second connecting portion 123 to threadedly connect with the bottle mouth of the liquid storage bottle, the liquid storage bottle is communicated with the containing cavity 111 of the bag body 110 through the liquid adding tube 120. When it is necessary to add liquid into the containing cavity 111 during the culture process, only need to lift the liquid storage bottle and invert it, so that the liquid in the liquid storage bottle can be automatically added into the containing cavity 111 of the bag body 110 in sequence through the bottle mouth of the liquid storage bottle, the liquid adding tube 120 and the first connecting tube 112 under the action of gravity. The operation is simple and convenient, saving time and effort. It avoids the technical problems in the prior art that because the volume of the liquid to be added is much larger than the volume of the syringe barrel, it is necessary to draw and inject multiple times, resulting in cumbersome operation and time-consuming and laborious.

[0038] By sleeving a first liquid stop member 130 on the first tube body 121 at one end of the first tube body 121 close to the first connection portion 122, when it is necessary to add liquid into the accommodation cavity 111, the operator first manually opens the first liquid stop member 130 to open the first tube body 121, and then raises and inverts the liquid storage bottle so that the liquid in the liquid storage bottle can be automatically added into the accommodation cavity 111 of the bag body 110 in sequence through the bottle mouth of the liquid storage bottle, the liquid adding tube 120 and the first connecting tube 112 under the action of gravity. After the liquid adding operation is completed, the operator manually closes the first liquid stop member 130 to close the first tube body 121, playing a role of sealing and isolation, effectively reducing the risk of the cell liquid in the accommodation cavity 111 being contaminated.

[0039] Such as Figure 1 As shown, in an embodiment of the present application, the multifunctional cell culture bag 100 further includes a second liquid stop member 140 and a controller, the controller is electrically connected to the second liquid stop member 140, the second liquid stop member 140 is sleeved on the first tube body 121, and is located at one end of the first tube body 121 close to the second connection portion 123.

[0040] In this embodiment, by sleeving a second liquid stop member 140 on the first tube body 121 at one end of the first tube body 121 close to the second connection portion 123 and electrically connecting the second liquid stop member 140 to the controller. In this way, when it is necessary to add liquid into the accommodation cavity 111, the operator first manually opens the first liquid stop member 130 to open one end of the first tube body 121 close to the first liquid stop member 130, and then raises and inverts the liquid storage bottle, and the controller controls the second liquid stop member 140 to open for a preset time so that one end of the first tube body 121 close to the second liquid stop member 140 is open for a preset time, so that the liquid in the liquid storage bottle is added into the accommodation cavity 111 of the bag body 110 with a preset volume under the action of gravity. After exceeding the preset time, the controller controls the second liquid stop member 140 to close to close one end of the first tube body 121 close to the second liquid stop member 140, realizing the functions of accurate liquid addition and automatic liquid stop, and ensuring that the added liquid volume is accurate.

[0041] In the above embodiment of the present application, the controller includes a time adjustment interface for adjusting the relaxation time of the second liquid stop member 140, and the time adjustment interface is configured with a plurality of time gears for adjusting time.

[0042] Thus, when it is necessary to add liquid into the accommodating cavity 111, an operator can operate the time adjustment interface to adjust the relaxation time of the second liquid stop member 140, thereby adjusting the opening time of one end of the first tube body 121 close to the second liquid stop member 140, and further adjusting the volume of the liquid added from the liquid storage bottle into the accommodating cavity 111. Meanwhile, the time adjustment interface is configured with multiple time gears for adjusting time, so as to facilitate the operator to select the corresponding time gear according to the volume of the liquid to be added into the accommodating cavity 111, realizing the functions of accurate liquid addition and automatic liquid stop, and ensuring the accuracy of the added liquid volume.

[0043] As Figure 2 shown, in the above-mentioned embodiment of the present application, the multifunctional cell culture bag 100 further includes a buffer container 150, the buffer container 150 is connected to the first tube body 121, and is located between the first liquid stop member 130 and the second liquid stop member 140.

[0044] In this embodiment, by arranging a buffer container 150 connected to the first tube body 121 between the first liquid stop member 130 and the second liquid stop member 140, a buffering and temporary storage effect is achieved, which is used to temporarily collect the liquid to be added into the accommodating cavity 111.

[0045] As Figure 1 shown, in an embodiment of the present application, the multifunctional cell culture bag 100 further includes a sampling member 160. One end of the accommodating cavity 111 close to the first connecting tube 112 has a second connecting tube 113 communicating with the accommodating cavity 111. The sampling member 160 includes an elastic bladder 161 and a third connecting portion 162. One end of the third connecting portion 162 is connected to the elastic bladder 161, and the other end is threadedly connected to the second connecting tube 113.

[0046] In this embodiment, one end of the accommodating cavity 111 close to the first connecting tube 112 has a second connecting tube 113 communicating with the accommodating cavity 111. By connecting one end of the third connecting portion 162 to the elastic bladder 161 and threadedly connecting the other end of the third connecting portion 162 to the second connecting tube 113, the elastic bladder 161 is communicated with the accommodating cavity 111 of the bag body 110 through the third connecting portion 162. Thus, when it is necessary to sample and detect the cell liquid in the accommodating cavity 111, an operator can repeatedly squeeze the elastic bladder 161 to suck the cell liquid in the accommodating cavity 111 into the elastic bladder 161 in sequence through the second connecting tube 113 and the third connecting portion 162 by using vacuum negative pressure. The sampling operation is simple, convenient and highly safe. It avoids the technical problems in the prior art that when sampling by inserting the needle of a syringe into the second connecting tube, it is easy to stab the operator or puncture the bag body.

[0047] In the above-mentioned embodiments of the present application, a volume scale mark is provided on the outer surface of the elastic bladder 161.

[0048] In this embodiment, by providing a volume scale mark on the outer surface of the elastic bladder 161, it is convenient for the operator to control the number of times of squeezing the elastic bladder 161 according to the volume scale mark to obtain the required volume of the sampled liquid, thereby realizing the function of accurate sampling.

[0049] As Figure 1 shown, in the above-mentioned embodiments of the present application, the sampling member 160 further includes a fourth connecting portion 163. One end of the fourth connecting portion 163 is connected to the elastic bladder 161, and the other end is used to be connected to the head of the sampling syringe barrel.

[0050] In this embodiment, by connecting one end of the fourth connecting portion 163 to the elastic bladder 161 and connecting the other end of the fourth connecting portion 163 to the head of the sampling syringe barrel, when it is necessary to sample and detect the cell liquid in the accommodating cavity 111, the operator can squeeze the elastic bladder 161 multiple times to use the vacuum negative pressure to sequentially suck the cell liquid in the accommodating cavity 111 into the elastic bladder 161 through the second connecting pipe 113 and the third connecting portion 162. Then, connect the head of the sampling syringe barrel to the fourth connecting portion 163 to draw the cell liquid in the elastic bladder 161 into the syringe barrel, so as to facilitate subsequent detection of the cell liquid.

[0051] As Figure 1 shown, in any one of the above-mentioned embodiments of the present application, the multifunctional cell culture bag 100 further includes a liquid extraction pipe 170. One end of the accommodating cavity 111 close to the first connecting pipe 112 has a third connecting pipe 114 communicating with the accommodating cavity 111. The liquid extraction pipe 170 includes a second pipe body 171, a fifth connecting portion 172 and a sixth connecting portion 173. The second pipe body 171 is respectively connected to the fifth connecting portion 172 and the sixth connecting portion 173. The fifth connecting portion 172 is threadedly connected to the third connecting pipe 114, and the sixth connecting portion 173 is used to be threadedly connected to the mouth of the centrifuge bottle.

[0052] In this embodiment, one end of the accommodation cavity 111 close to the first connecting pipe 112 has a third connecting pipe 114 communicating with the accommodation cavity 111. The second pipe body 171 is respectively connected to the fifth connecting part 172 and the sixth connecting part 173. By threadedly connecting the fifth connecting part 172 to the third connecting pipe 114 and using the sixth connecting part 173 to be threadedly connected to the bottle mouth of the centrifuge bottle, the centrifuge bottle is communicated with the accommodation cavity 111 of the bag body 110 through the liquid extraction pipe 170. When the liquid in the accommodation cavity 111 needs to be taken out after the culture is completed, only need to lift and invert the bag body 110 so that the liquid in the accommodation cavity 111 can be automatically added into the centrifuge bottle successively through the third connecting pipe 114 and the liquid extraction pipe 170 under the action of gravity. The operation is simple and convenient, saving time and effort.

[0053] As Figure 3 shown, in the above-mentioned embodiment of the present application, the multifunctional cell culture bag 100 further includes a fixing member 180. The fixing member 180 is arranged on one side of the bag body 110. The fixing member 180 is provided with a first clamping groove 181 adapted to the first pipe body 121 and a second clamping groove 182 adapted to the second pipe body 171.

[0054] In this embodiment, by arranging the fixing member 180 on one side of the bag body 110 and respectively providing the first clamping groove 181 and the second clamping groove 182 adapted to the first pipe body 121 and the second pipe body 171 on the fixing member 180, when not in use, the first pipe body 121 and the second pipe body 171 are respectively clamped in the first clamping groove 181 and the second clamping groove 182, so that the liquid adding pipe 120 and the liquid extraction pipe 170 are firmly attached to the bag body 110, realizing the storage and fixing functions of the liquid adding pipe 120 and the liquid extraction pipe 170, facilitating the taking and placing of the multifunctional cell culture bag 100, and reducing the risk of contamination of the liquid adding pipe 120 and the liquid extraction pipe 170.

[0055] Embodiment Two

[0056] The embodiment of the present application further provides a cell culture device, including the multifunctional cell culture bag 100 described in the above embodiment.

[0057] This cell culture device has the multifunctional cell culture bag 100 in any of the above embodiments, so it has all the beneficial effects of the multifunctional cell culture bag 100, which will not be elaborated one by one here.

[0058] In the description of this specification, the description referring to terms such as "one embodiment", "some embodiments", "examples", "specific examples", or "some examples", etc. means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of this application. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0059] Although the embodiments of this application have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting this application. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of this application.

Claims

1. A multifunctional cell culture bag, characterized in that Comprising: A bag body having a receiving cavity, one end of the receiving cavity having a first connecting pipe communicating with the receiving cavity; A liquid adding pipe, the liquid adding pipe comprising a first pipe body, a first connecting portion and a second connecting portion, the first pipe body being respectively connected to the first connecting portion and the second connecting portion, the first connecting portion being threadedly connected to the first connecting pipe, and the second connecting portion being used for threadedly connecting to the bottle mouth of a liquid storage bottle; A first liquid stopping member sleeved on the first pipe body and located at one end of the first pipe body close to the first connecting portion.

2. The multifunctional cell culture bag according to claim 1, characterized in that, The multifunctional cell culture bag further comprises a second liquid stopping member and a controller, the controller being electrically connected to the second liquid stopping member, the second liquid stopping member being sleeved on the first pipe body and located at one end of the first pipe body close to the second connecting portion.

3. The multifunctional cell culture bag according to claim 2, characterized in that, The controller comprises a time adjustment interface for adjusting the relaxation time of the second liquid stopping member, and the time adjustment interface is configured with a plurality of time gears for adjusting time.

4. The multifunctional cell culture bag according to claim 2, characterized in that, The multifunctional cell culture bag further comprises a buffer container connected to the first pipe body and located between the first liquid stopping member and the second liquid stopping member.

5. The multifunctional cell culture bag according to claim 1, characterized in that, The multifunctional cell culture bag further comprises a sampling member, one end of the receiving cavity close to the first connecting pipe having a second connecting pipe communicating with the receiving cavity, the sampling member comprising an elastic bladder and a third connecting portion, one end of the third connecting portion being connected to the elastic bladder and the other end being threadedly connected to the second connecting pipe.

6. The multi-functional cell culture bag according to claim 5, characterized in that, The outer surface of the elastic bladder is provided with volume scale marks.

7. The multifunctional cell culture bag according to claim 5, characterized in that, The sampling member further comprises a fourth connecting portion, one end of the fourth connecting portion being connected to the elastic bladder and the other end being used for connecting to the head of a sampling syringe barrel.

8. The multifunctional cell culture bag according to any one of claims 1 to 7, characterized in that, The multifunctional cell culture bag further comprises a liquid taking pipe, one end of the receiving cavity close to the first connecting pipe having a third connecting pipe communicating with the receiving cavity, the liquid taking pipe comprising a second pipe body, a fifth connecting portion and a sixth connecting portion, the second pipe body being respectively connected to the fifth connecting portion and the sixth connecting portion, the fifth connecting portion being threadedly connected to the third connecting pipe, and the sixth connecting portion being used for threadedly connecting to the bottle mouth of a centrifuge bottle.

9. The multifunctional cell culture bag according to claim 8, wherein, The multifunctional cell culture bag further comprises a fixing member provided on one side of the bag body, the fixing member being provided with a first card slot adapted to the first pipe body and a second card slot adapted to the second pipe body.

10. A cell culture device, characterized in that, Comprising the multifunctional cell culture bag according to any one of claims 1 to 9.

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