Detection device for simultaneously detecting various pollutants
By designing the structure of the detection disk and detection tank, simultaneous detection of multiple pollutants is achieved, which solves the limitations of single pollutant detection in the prior art, improves detection efficiency and simplifies the operation process.
Patent Information
- Application Number
- CN202421485602.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-26
- Publication Date
- 2025-07-11
- Estimated Expiration
- 2034-06-26
AI Technical Summary
The existing rapid detection methods can only detect a single pollutant and are difficult to meet the detection needs of complex substrates where multiple pollutants exist together.
A detection device is designed, including a detection disk, a sample groove and a plurality of detection grooves evenly arranged in the circumference. Specific test strips are arranged in the groove. The detection liquid flows into the groove through capillary action, so as to realize the simultaneous detection of multiple pollutants.
It significantly improves detection efficiency, simplifies operational steps, reduces cost and time, and can obtain detection results of multiple pollutants in one experiment.
Smart Images
Figure CN223091966U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the field of pollutant detection equipment, and in particular to a detection device for simultaneously detecting multiple pollutants. Background Art
[0002] Pollutant detection has important applications in many fields. For example, in agricultural production, in order to increase production and ensure the healthy growth of crops, pesticides are widely used to prevent and control pests and diseases. However, after the use of pesticides, it is inevitable that residues will remain in food, fruits and vegetables, which will cause potential harm to human health. In the field of food safety, it is necessary to detect heavy metals, toxins, microorganisms and other harmful substances in food to ensure food safety. In environmental monitoring, the detection of pollutants in water quality, atmosphere, and soil, including heavy metals, industrial waste, organic pollutants, etc., helps to evaluate and manage environmental quality. In medical diagnosis, the detection of biomarkers in human body fluids, such as disease markers in blood and urine, can assist in the early diagnosis and monitoring of diseases. In industrial production, it is necessary to detect impurities and pollutants in raw materials and products to ensure the quality control of the production process and the qualification of products. In the field of public health, it is necessary to detect harmful substances in drinking water, pollutants in the air, harmful microorganisms in public places, etc., to ensure public health safety.
[0003] In the prior art, pollutant detection is usually performed by instrumental detection methods and rapid detection methods. Instrumental detection methods include gas chromatography, liquid chromatography, gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, etc. Although these methods have accurate results and a wide range of applications, they have the disadvantages of complex sample pretreatment, long time consumption and high cost, and are difficult to meet the needs of on-site rapid detection. In contrast, rapid detection methods include enzyme inhibition methods, immunoassays and biosensors, etc. These methods are simple to operate, have high analysis efficiency, and can provide test results in a short time. However, existing rapid detection methods can generally only detect a single pollutant, and there are limitations in complex matrices where multiple pollutants coexist, which makes it difficult to meet the needs of practical applications. Utility Model Content
[0004] To this end, the present application provides a detection device for the simultaneous detection of multiple pollutants to solve the problem of single pollutant detection in the prior art rapid detection method.
[0005] In order to achieve the above objectives, this application provides the following technical solutions:
[0006] A detection device for simultaneous detection of multiple pollutants, comprising a detection disc, a sample circular groove opened at the center of the detection disc and used for accommodating a detection liquid, and a plurality of detection grooves opened on the detection disc and uniformly arranged along the circumferential direction of the sample circular groove. A test strip is arranged in the detection groove. One end of the detection groove communicates with the sample circular groove, and the other end penetrates to the edge of the detection disc.
[0007] Optionally, a first limiting plate is connected to the groove wall at one end of the detection groove close to the sample circular groove. A first gap for the test strip to pass through is formed between the first limiting plate and the bottom of the detection groove, and the top of the first limiting plate is flush with the surface of the detection disc.
[0008] Optionally, a plurality of limiting blocks for restricting the test strip from moving towards the center of the detection disc are connected to the bottom of the sample circular groove, and the plurality of limiting blocks correspond to the plurality of detection grooves one by one.
[0009] Optionally, a sample ring for cooperating with the sample circular groove to expand the detection liquid accommodation volume of the sample circular groove is connected to the detection disc at the notch of the sample circular groove, and the inner ring surface of the sample ring is flush with the side wall of the detection groove.
[0010] Optionally, a second limiting plate is connected to the bottom of the sample ring at the detection groove, and a second gap for the test strip to pass through is formed between the second limiting plate and the bottom of the detection groove.
[0011] Optionally, the sample ring and the detection disc are integrally formed, the sample ring and the second limiting plate are integrally formed, and the detection disc and the second limiting plate are integrally formed.
[0012] Optionally, the diameter of the sample circular groove is 1.6 cm, the sum of the ring height of the sample ring and the depth of the sample circular groove is 5 mm, and the detection groove is perpendicular to the groove wall of the sample circular groove; the width of the detection groove is 4.5 mm, the depth of the detection groove is 2 mm, and the length of the detection groove is 4.3 cm; the distance between the limiting block and the notch of the detection groove is 1 mm; the width of the test strip is 4 mm, the thickness of the test strip is 0.5 mm, a 1.7 cm water absorption pad is connected to one end of the test strip away from the sample circular groove, and the total length of the test strip and the water absorption pad is 6 cm.
[0013] Compared with the prior art, the present application has at least the following beneficial effects:
[0014] The experimenter adds the detection liquid into the sample circular groove, and the detection liquid flows from the sample circular groove into multiple detection grooves evenly arranged circumferentially simultaneously through gravity and capillary action. Each detection groove is pre-arranged with a specific test strip, and each type of test strip can specifically react with different pollutants. As the detection liquid flows in the detection groove, the detection area on the test strip will come into contact with the pollutants in the detection liquid, triggering an immune reaction or other chemical reactions, and then showing different detection results. Through this design, the device can detect multiple different pollutants simultaneously, significantly improving the detection efficiency. By using multiple detection grooves to work synchronously, the detection results of multiple pollutants can be obtained in one experiment, avoiding the cumbersome process of multiple operations, simplifying the detection steps, and reducing the operation cost and time. In addition, one end of the detection groove is connected to the sample circular groove, and the other end penetrates through to the edge of the detection disc. This design enables the test strip to be pushed into the detection groove and the sample circular groove along the detection groove from the edge of the detection disc, facilitating the installation and replacement of the test strip. Description of the Drawings
[0015] To more intuitively illustrate the prior art and this application, several exemplary drawings are given below. It should be understood that the specific shapes and structures shown in the drawings generally should not be regarded as limiting conditions when implementing this application; for example, those skilled in the art are capable of making routine adjustments or further optimizations to the addition / removal / attribution division of certain units (components), specific shapes, positional relationships, connection methods, dimensional ratio relationships, etc. based on the technical concept disclosed in this application and the exemplary drawings.
[0016] Figure 1 Schematic diagram of the overall structure of the detection disc of a detection device for simultaneous detection of multiple pollutants provided by an embodiment of this application;
[0017] Figure 2 For Figure 1 top view;
[0018] Figure 3 For Figure 2 enlarged view of part A of
[0019] Figure 4 For Figure 1 another perspective view of
[0020] Figure 5 For Figure 4 enlarged view of part B of
[0021] Figure 6 For Figure 1 side view of
[0022] Figure 7Schematic diagram of the structure of the detection disc and test strip of a detection device for simultaneous detection of multiple pollutants provided by an embodiment of the present application;
[0023] Figure 8 For Figure 7 Enlarged view of part C.
[0024] Explanation of reference numerals:
[0025] 1. Detection disc; 11. Sample circular groove; 111. Limit block; 12. Detection groove; 121. First gap; 122. Second gap; 13. First limit plate; 2. Sample ring; 21. Second limit plate; 3. Test strip; 31. Absorbent pad. Detailed implementation manners
[0026] In order to make the objectives, technical solutions and advantages of the present application clearer, the present application will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.
[0027] In the description of the present application: Unless otherwise specified, "a plurality of" means two or more. Terms such as "first", "second", "third", etc. in the present application are intended to distinguish the objects being referred to and do not have special significance in terms of technical connotations (for example, they should not be understood as emphasizing the importance or order, etc.). Expressions such as "including", "comprising", "having", etc. also mean "not limited to" (certain units, components, materials, steps, etc.).
[0028] Refer to Figure 1-8 , the present application discloses a detection device for simultaneous detection of multiple pollutants, including a detection disc 1, a sample circular groove 11 opened at the center of the detection disc 1 and used for accommodating a detection liquid, and a plurality of detection grooves 12 opened on the detection disc 1 and evenly arranged along the circumferential direction of the sample circular groove 11. A test strip 3 is arranged in the detection groove 12. One end of the detection groove 12 communicates with the sample circular groove 11, and the other end penetrates to the edge of the detection disc 1.
[0029] The experimenter adds the detection liquid into the sample circular groove 11. The detection liquid flows from the sample circular groove 11 into multiple detection grooves 12 that are evenly arranged circumferentially simultaneously through gravity and capillary action. Each detection groove 12 is pre-arranged with a specific test strip 3, and each test strip 3 can specifically react with different pollutants. As the detection liquid flows in the detection groove 12, the detection area on the test strip 3 will come into contact with the pollutants in the detection liquid, triggering an immune reaction or other chemical reactions, and then showing different detection results. Through this design, the device can detect multiple different pollutants simultaneously, significantly improving the detection efficiency. By using multiple detection grooves 12 to work synchronously, the detection results of multiple pollutants can be obtained in one experiment, avoiding the cumbersome process of multiple operations, simplifying the detection steps, and reducing the operation cost and time. In addition, one end of the detection groove 12 is connected to the sample circular groove 11, and the other end penetrates to the edge of the detection disc 1. This design enables the test strip 3 to be pushed into the detection groove 12 and the sample circular groove 11 along the detection groove 12 from the edge of the detection disc 1, facilitating the installation and replacement of the test strip 3.
[0030] It should be explained that the detection liquid can be a sample extraction liquid, and the sample extraction liquid can be a liquid extracted from foods, agricultural products, water samples, blood, etc.
[0031] A first limiting plate 13 is connected to the groove wall at one end of the detection groove 12 close to the sample circular groove 11. A first gap 121 that facilitates the test strip 3 to pass through is formed between the first limiting plate 13 and the bottom of the detection groove 12, and the top of the first limiting plate 13 is flush with the surface of the detection disc 1.
[0032] The design of the first limiting plate 13 can effectively limit the position of the test strip 3 in the detection groove 12. The experimenter pushes the test strip 3 along the detection groove 12 from the edge of the detection disc 1, and the test strip 3 enters the detection groove 12 through the first gap 121 at the first limiting plate 13 and extends to the sample circular groove 11. Since the first limiting plate 13 is flush with the surface of the detection disc 1, the test strip 3 remains flat in the groove, which helps the detection liquid to be evenly distributed on the test strip 3 and avoids detection errors caused by the sliding or movement of the test strip 3.
[0033] A plurality of limiting blocks 111 for restricting the test strip 3 from moving towards the center of the detection disc 1 are connected to the bottom of the sample circular groove 11, and the plurality of limiting blocks 111 correspond to the plurality of detection grooves 12 one by one. The limiting blocks 111 prevent the test strip 3 from continuing to move towards the center of the detection disc 1, ensuring the accurate positioning of the test strip 3 in the detection groove 12. Since the limiting blocks 111 correspond to the detection grooves 12 one by one, the test strip 3 can remain stable in each detection groove 12, avoiding the situation of position deviation of the test strip 3 during the detection process.
[0034] A sample ring 2 for cooperating with the sample circular groove 11 to expand the detection liquid accommodation volume of the sample circular groove 11 is connected to the detection disc 1 at the notch of the sample circular groove 11, and the inner ring surface of the sample ring 2 is flush with the side wall of the detection groove 12.
[0035] The design of the sample ring 2 expands the accommodation volume of the sample circular groove 11, so that more detection liquid can be accommodated. This structural design enables the experimenter to add more detection liquid into the sample circular groove 11 to ensure that the detection liquid can fully flow into each detection groove 12.
[0036] A second limiting plate 21 is connected to the bottom of the sample ring 2 at the detection groove 12, and a second gap 122 for facilitating the test strip 3 to pass through is formed between the second limiting plate 21 and the bottom of the detection groove 12.
[0037] The design of the second limiting plate 21 optimizes the flow path of the detection liquid. When the detection liquid enters the detection groove 12 from the sample circular groove 11 through the second gap 122, the flow speed is restricted. The second gap 122 narrows the flow channel from the sample circular groove 11 to the detection groove 12, preventing the detection liquid from flowing quickly above the test strip 3, making the flow speed of the detection liquid on the test strip 3 slow, thus promoting the uniform chromatography of the detection liquid on the test strip 3. This slow chromatography process helps the detection liquid to fully contact and react with the antibodies on the test strip 3, ensuring the accuracy of the detection results.
[0038] The sample ring 2 is integrally formed with the detection disc 1, the sample ring 2 is integrally formed with the second limiting plate 21, and the detection disc 1 is integrally formed with the second limiting plate 21. The whole pollutant detection device is formed by 3D printing with photosensitive resin.
[0039] The diameter of the sample circular groove 11 is 1.6 cm, the sum of the ring height of the sample ring 2 and the depth of the sample circular groove 11 is 5 mm, the detection groove 12 is perpendicular to the side wall of the sample circular groove 11, the number of the detection grooves 12 is 8, the pollutant detection device can accommodate 1 mL of detection liquid, meeting the injection volume requirement of 50 - 100 μL for each of the 8 branch channels; the width of the detection groove 12 is 4.5 mm, the depth of the detection groove 12 is 2 mm, and the length of the detection groove 12 is 4.3 cm; the distance between the limiting block 111 and the notch of the detection groove 12 is 1 mm; the width of the test strip 3 is 4 mm, the thickness of the test strip 3 is 0.5 mm, a water absorption pad 31 with a length of 1.7 cm is connected to one end of the test strip 3 far from the sample circular groove 11, the total length of the test strip 3 and the water absorption pad 31 is 6 cm, and a part of the water absorption pad 31 extends out of the detection disc 1, and the water absorption pad 31 can absorb the excess detection liquid.
[0040] Taking pesticide residues as an example, the detection performance of the detection device was investigated. First, the parallelism among 8 channels of the same device was examined. Eight carbofuran immunochromatographic test strips were placed in parallel in the same detection disc device. Detection solutions with carbofuran concentrations of 0, 0.02, and 0.04 mg / kg were added to the sample round grooves. The T / C values were obtained and the average values and relative standard deviations were calculated. As shown in Table 1, the relative standard deviations of the detections of 8 channels in each sample disc were less than 20%, indicating good parallelism of the 8 channels of the detection device.
[0041] Table 1 - Detection Results of Carbofuran at Different Concentrations
[0042]
[0043] Secondly, the reproducibility among multiple devices was investigated. Detection test strips for cyromazine, clothianidin, chlorantraniliprole, isocarbophos, thiamethoxam, profenofos, prochloraz, and methomyl were successively placed in 8 detection channels of the same detection disc device. Detection solutions with blank and 1-fold limit concentrations were added to the sample round grooves. The T / C values were obtained and the average values and relative standard deviations were calculated. As shown in Table 2 and Table 3, the relative standard deviations of the detections of each pesticide residue among the three sample discs were less than 25%, indicating good repeatability of the detection device and it can meet the actual use requirements.
[0044] Table 2 - Simultaneous Detection Results of 8 Kinds of Pesticide Residues (Blank)
[0045]
[0046] Table 3 - Simultaneous Detection Results of 8 Kinds of Pesticide Residues (1-fold Limit)
[0047]
[0048] The technical features of the above embodiments can be combined arbitrarily (as long as there is no contradiction in the combination of these technical features). For the sake of concise description, not all possible combinations of the technical features in the above embodiments are described; these embodiments not explicitly written out should also be regarded as within the scope described in this specification.
[0049] In the above text, the present application has been described in a relatively specific and detailed manner through general descriptions and specific embodiments. It should be understood that based on the technical concept of the present application, several conventional adjustments or further innovations can be made to these specific embodiments; but as long as they do not depart from the technical concept of the present application, the technical solutions obtained by these conventional adjustments or further innovations also fall within the protection scope of the claims of the present application.
Claims
1. A detection device for simultaneous detection of multiple pollutants, characterized in that, It includes a detection disc, a sample circular groove opened at the center of the detection disc and used for accommodating a detection liquid, and a plurality of detection grooves opened on the detection disc and uniformly arranged along the circumferential direction of the sample circular groove. A test strip is arranged in the detection groove. One end of the detection groove communicates with the sample circular groove, and the other end penetrates to the edge of the detection disc.
2. The detection device according to claim 1, wherein A first limiting plate is connected to the groove wall of one end of the detection groove close to the sample circular groove. A first gap for the test strip to pass through is formed between the first limiting plate and the bottom of the detection groove. The top of the first limiting plate is flush with the surface of the detection disc.
3. The detection device according to claim 2, wherein A plurality of limiting blocks for restricting the test strip from moving towards the center of the detection disc are connected to the bottom of the sample circular groove. The plurality of limiting blocks correspond to the plurality of detection grooves one by one.
4. The detection device according to claim 3, wherein A sample ring for cooperating with the sample circular groove to expand the detection liquid accommodation volume of the sample circular groove is connected to the detection disc at the notch of the sample circular groove. The inner ring surface of the sample ring is flush with the side wall of the detection groove.
5. The detection device according to claim 4, characterized in that, A second limiting plate is connected to the bottom of the sample ring at the detection groove. A second gap for the test strip to pass through is formed between the second limiting plate and the bottom of the detection groove.
6. The detection device according to claim 5, characterized in that, The sample ring and the detection disc are integrally formed. The sample ring and the second limiting plate are integrally formed. The detection disc and the second limiting plate are integrally formed.
7. The detection device according to claim 5, characterized in that, The diameter of the sample circular groove is 1.6 cm. The sum of the ring height of the sample ring and the depth of the sample circular groove is 5 mm. The detection groove is perpendicular to the groove wall of the sample circular groove. The number of the detection grooves is 8. The width of the detection groove is 4.5 mm. The depth of the detection groove is 2 mm. The length of the detection groove is 4.3 cm. The distance between the limiting block and the notch of the detection groove is 1 mm. The width of the test strip is 4 mm. The thickness of the test strip is 0.5 mm. A 1.7-cm water-absorbing pad is connected to one end of the test strip far from the sample circular groove. The total length of the test strip and the water-absorbing pad is 6 cm.