Cross liquid transfer pipeline

Abstract

The utility model relates to a cross-shaped liquid transfer pipeline which comprises a cross-shaped pipeline, the cross-shaped pipeline comprises a transverse pipeline, and the two ends of the transverse pipeline are a liquid inlet end used for being communicated with a culture medium and a liquid outlet end used for being communicated with a cell bag respectively; the two ends of the longitudinal pipeline are an injection end used for being communicated with positive and negative pressure blowing and sucking equipment and an air end used for being communicated with outside air respectively; the transverse pipeline and the longitudinal pipeline are intersected and communicated to form a cross intersection part; a first pipeline clamp is arranged on the pipeline between the cross-shaped intersection part and the liquid inlet end, a second pipeline clamp is arranged on the pipeline between the cross-shaped intersection part and the liquid outlet end, and a third pipeline clamp is arranged on the pipeline between the cross-shaped intersection part and the air end and used for controlling opening and closing of the pipelines. According to the structure, the problem that the liquid supplementing volume is not accurate enough can be effectively solved, the using process is totally closed and sterile, and the practicability is very high.

Description

Technical Field

[0001] The utility model relates to the field of cell culture equipment, and particularly relates to a cross liquid transfer pipeline. Background Art

[0002] Cell culture tends to be fully enclosed or semi-closed, and liquid supplement becomes a key step. At present, there are two types of fully enclosed liquid supplements: an automatic liquid transfer instrument and an electronic scale for weighing. The precise liquid transfer conditions of the automatic liquid transfer instrument are harsh, and it is greatly affected by the liquid pressure at both ends and the smoothness of the pipeline; the electronic scale is not accurate enough for a small amount of liquid supplement, and the volume and weight are converted according to the ratio of 1g = 1ml, resulting in a relatively large error.

[0003] Under fully enclosed conditions, a liquid transfer pipeline is needed to effectively solve the problem of inaccurate liquid supplement volume. Summary of the Utility Model

[0004] The purpose of the utility model is to overcome the problems existing in the prior art and provide a cross liquid transfer pipeline.

[0005] To achieve the above technical purpose and reach the above technical effect, the utility model is realized through the following technical solutions:

[0006] A cross liquid transfer pipeline includes a cross pipeline, and the cross pipeline includes:

[0007] A horizontal pipeline, with the two ends of the horizontal pipeline being a liquid inlet end for connecting a culture medium and a liquid outlet end for connecting a cell bag respectively;

[0008] A vertical pipeline, with the two ends of the vertical pipeline being an injection end for connecting a positive and negative pressure blowing and sucking device and an air end for connecting the outside air respectively;

[0009] The horizontal pipeline and the vertical pipeline intersect and communicate to form a cross intersection;

[0010] A first pipeline clamp is provided on the pipeline between the cross intersection and the liquid inlet end, a second pipeline clamp is provided on the pipeline between the cross intersection and the liquid outlet end, and a third pipeline clamp is provided on the pipeline between the cross intersection and the air end for controlling the opening and closing of the pipeline.

[0011] Further, a fully enclosed liquid transfer cavity is provided on the pipeline between the cross intersection and the injection end, a piston is slidably provided in the fully enclosed liquid transfer cavity, and the piston is pushed by the air pressure generated by the positive and negative pressure blowing and sucking device.

[0012] Further, a first air filter is provided at the blowing and sucking port of the fully enclosed liquid transfer cavity for sterilization and filtration.

[0013] Further, a second air filter is provided at the air end for sterilization filtration. An air chamber is provided on the pipeline between the second air filter and the third pipeline clamp as a buffer zone when adjusting the injection volume to prevent wetting the second air filter.

[0014] Further, female Luer ports are provided at the liquid inlet end and the injection end, and a male Luer port is provided at the liquid outlet end as a pipeline interface.

[0015] The beneficial effects of the present utility model are as follows:

[0016] The structure of the present utility model can effectively solve the problem of inaccurate fluid replacement volume, and the whole process during use is fully enclosed and sterile, with very high practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a schematic structural diagram of Embodiment 1 of the present utility model;

[0018] Figure 2 is a schematic structural diagram of Embodiment 2 of the present utility model.

[0019] Explanation of the reference numerals in the drawings: 1. First pipeline clamp, 2. Second pipeline clamp, 3. Third pipeline clamp, 4. Cross intersection, 5. Fully enclosed liquid transfer cavity, 6. Piston, 7. First air filter, 8. Second air filter, 9. Air chamber, 10. Female Luer port, 11. Male Luer port, 12. Syringe. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0020] The present utility model will be described in detail below with reference to the drawings and in conjunction with the embodiments.

[0021] Embodiment 1

[0022] As Figure 1 shown, a cross liquid transfer pipeline includes a cross pipeline, and the cross pipeline includes:

[0023] A horizontal pipeline, with the two ends of the horizontal pipeline being a liquid inlet end for connecting the culture medium and a liquid outlet end for connecting the cell bag respectively;

[0024] A vertical pipeline, with the two ends of the vertical pipeline being an injection end for connecting the positive and negative pressure blowing and sucking equipment and an air end for connecting the external air respectively;

[0025] The horizontal pipeline and the vertical pipeline intersect and communicate to form a cross intersection 4;

[0026] A first pipeline clamp 1 is provided on the pipeline between the cross intersection 4 and the liquid inlet end, a second pipeline clamp 2 is provided on the pipeline between the cross intersection 4 and the liquid outlet end, and a third pipeline clamp 3 is provided on the pipeline between the cross intersection 4 and the air end for controlling the opening and closing of the pipeline.

[0027] A second air filter 8 is provided at the air end for sterilization filtration. An air chamber 9 is provided on the pipeline between the second air filter 8 and the third pipeline clamp 3 as a buffer zone when adjusting the injection volume to prevent the second air filter 8 from being wetted.

[0028] Female Luer ports 10 are provided at the liquid inlet end and the injection end, and a male Luer port 11 is provided at the liquid outlet end as a pipeline interface.

[0029] Embodiment 2

[0030] As Figure 2 shown, compared with Embodiment 1, the difference is that a fully enclosed liquid transfer cavity 5 is provided on the pipeline between the cross intersection part 4 and the injection end. Different volume specifications such as 10 ml, 20 ml, 50 ml, 100 ml, 200 ml, etc. can be adopted. Volume scales can be set on the fully enclosed liquid transfer cavity 5. A piston 6 is slidably provided in the fully enclosed liquid transfer cavity 5. The piston 6 is pushed by the air pressure generated by a positive and negative pressure blowing and suction device to ensure full enclosure and sterility during use, greatly enhancing the practicability. A common syringe 12 can be selected as the positive and negative pressure blowing and suction device.

[0031] A first air filter 7 is provided at the blowing and suction port of the fully enclosed liquid transfer cavity 5. An air filter with a specification of 0.22 um can be selected for sterilization filtration.

[0032] The usage process of the present utility model

[0033] 1. Connect the culture medium to the liquid inlet end, connect the cell bag to the liquid outlet end, and connect the corresponding positive and negative pressure blowing and suction device to the injection end;

[0034] 2. Close the second pipeline clamp 2 and the third pipeline clamp 3, open the first pipeline clamp 1, and suck in the required volume of culture medium;

[0035] 3. Close the second pipeline clamp 2, and alternately open and close the first pipeline clamp 1 and the third pipeline clamp 3 to exhaust air, adjust the volume, and drain the liquid in the pipeline;

[0036] 4. Close the first pipeline clamp 1 and the third pipeline clamp 3, open the second pipeline clamp 2, and inject the culture medium into the cell culture bag;

[0037] 5. Close the first pipeline clamp 1, and alternately open and close the third pipeline clamp 3 and the second pipeline clamp 2 to suck in air and drain the liquid in the pipeline;

[0038] 6. Repeat the process of 1 - 5 to continue fluid infusion;

[0039] 7. Thermally combine and disconnect the liquid inlet pipeline and the liquid outlet pipeline of the horizontal pipeline to end the fluid infusion;

[0040] 8. Specialized automation equipment can also be designed for cooperative use.

[0041] In addition, it should be noted that unless otherwise specified or indicated, the terms "horizontal", "vertical", "first", "second", etc. in the specification are only used to distinguish the various components, elements, steps, etc. in the specification, rather than to represent the logical relationship or sequential relationship, etc. between the various components, elements, steps.

[0042] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A cross-transfer liquid pipeline, including a cross pipeline, characterized in that, The cross-shaped pipeline includes: A horizontal pipeline, with the two ends of the horizontal pipeline being a liquid inlet end for connecting the culture medium and a liquid outlet end for connecting the cell bag respectively; A vertical pipeline, with the two ends of the vertical pipeline being an injection end for connecting the positive and negative pressure blowing and suction device and an air end for connecting the external air respectively; The horizontal pipeline and the vertical pipeline intersect and communicate to form a cross intersection part (4); A first pipeline clamp (1) is provided on the pipeline between the cross intersection part (4) and the liquid inlet end, a second pipeline clamp (2) is provided on the pipeline between the cross intersection part (4) and the liquid outlet end, and a third pipeline clamp (3) is provided on the pipeline between the cross intersection part (4) and the air end, for controlling the opening and closing of the pipeline.

2. The cross-transfer liquid pipeline according to claim 1, characterized in that A fully enclosed liquid transfer cavity (5) is provided on the pipeline between the cross intersection part (4) and the injection end, a piston (6) is slidably provided in the fully enclosed liquid transfer cavity (5), and the piston (6) is pushed by the air pressure generated by the positive and negative pressure blowing and suction device.

3. The cross-transfer liquid pipeline according to claim 2, wherein A first air filter (7) is provided at the blowing and suction port of the fully enclosed liquid transfer cavity (5) for sterilization filtration.

4. The cross-transfer liquid pipeline according to claim 1 or 3, characterized in that, A second air filter (8) is provided at the air end for sterilization filtration, and an air chamber (9) is provided on the pipeline between the second air filter (8) and the third pipeline clamp (3) as a buffer zone when adjusting the injection volume to prevent wetting the second air filter (8).

5. The cross-flow liquid pipeline according to claim 4, wherein Female Luer ports (10) are provided at the liquid inlet end and the injection end, and male Luer ports (11) are provided at the liquid outlet end as pipeline interfaces.

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