Multifunctional hyperspectral imaging analysis system suitable for multiple application fields

Abstract

The utility model discloses a multifunctional hyperspectral imaging analysis system suitable for multiple application fields, which relates to the technical field of hyperspectral imaging analysis and comprises a mainframe box, and a master control system, an imaging light source, a light source support, an automatic sample conveying platform, a lifting shaft and a multifunctional imaging unit are integrated on the upper portion of the mainframe box. An on-line analysis module and a computer host are integrated on the lower portion of the mainframe box, the mainframe box is a totally-closed box body, and a completely dark condition is achieved after all box doors are closed. Reflected light hyperspectral imaging analysis, multi-excitation light excitation chlorophyll fluorescence hyperspectral imaging analysis and ultraviolet excitation biological fluorescence hyperspectral imaging analysis of an experimental sample are achieved through the hyperspectral multifunctional imaging unit, and reflection and biological fluorescence hyperspectrums with specific spectral characteristics can be obtained through the imaging unit by converting different imaging light sources. The spectral characteristics not only reflect phenotypic characteristics such as plant physiology and ecology, but also widen the research range of plant bioluminescence.

Description

Technical Field

[0001] The utility model relates to the technical field of hyperspectral imaging analysis, and particularly relates to a multifunctional hyperspectral imaging analysis system applicable to multiple application fields. Background Art

[0002] With the rapid development of modern agriculture, biotechnology and environmental science, the demand for efficient, non-destructive, accurate and comprehensive plant sample detection technologies is increasing day by day. Traditional detection methods have deficiencies in quantitative analysis and non-contact detection, unable to provide real-time, non-destructive and accurate data, and often difficult to comprehensively and accurately reflect the physiological state of plants.

[0003] Hyperspectral imaging products on the market are usually limited to the imaging analysis of the reflected light on the surface of plants; chlorophyll fluorescence imaging technology focuses on the temporal dynamic changes of chlorophyll fluorescence, lacking in-depth analysis of the fluorescence spectral characteristics; UV-MCF imaging is mainly multi-spectral imaging analysis, and only two-dimensional imaging analysis can be performed on the fluorescence (multi-spectral fluorescence) at four wavelengths of F440, F520, F690, and F740, but the characteristic peaks of different samples are also different. In addition, although the chlorophyll fluorescence spectrum and UV-MCF bioluminescence spectrum contain rich information, there is currently no commercial instrument that can provide hyperspectral imaging and analysis of chlorophyll fluorescence and bioluminescence, and there is a lack of products that can comprehensively analyze the plant phenotype from hyperspectral imaging, multi-excitation light-excited chlorophyll fluorescence hyperspectral imaging and bioluminescence hyperspectral imaging.

[0004] Based on this, a multifunctional hyperspectral imaging analysis system that can comprehensively analyze the physical and chemical properties of plants, photosynthetic phenotypes, biochemical components, physiological states, food, and the quality of traditional Chinese medicines, integrating reflected hyperspectral imaging, multi-excitation light-excited chlorophyll fluorescence hyperspectral imaging and ultraviolet light-excited bioluminescence hyperspectral imaging, is crucial for the comprehensive research of plant phenotypes, detection and identification of germplasm resources, crop genetic breeding, food, and detection and identification of the quality of traditional Chinese medicines. Summary of the Utility Model

[0005] (1) Technical Problems to be Solved

[0006] Aiming at the deficiencies of the prior art, the utility model provides a multifunctional hyperspectral imaging analysis system applicable to multiple application fields, which solves the technical problems that chlorophyll fluorescence cannot be analyzed in the spectral dimension, bioluminescence cannot be decoded comprehensively from multiple angles, the multifunctional hyperspectral imaging analysis applicable to multiple application fields that cannot simultaneously take into account reflected light hyperspectral, multi-excitation light chlorophyll fluorescence hyperspectral and UV-MCF bioluminescence hyperspectral imaging, and the results cannot be further analyzed and displayed in real time.

[0007] (2) Technical Solutions

[0008] To achieve the above objectives, the utility model is realized through the following technical solutions:

[0009] A multi-functional hyperspectral imaging analysis system applicable to multiple application fields, including a main chassis. The upper part of the main chassis is integrated with a main control system, an imaging light source, a light source support, an automatic sample transfer platform, a lifting shaft, and a multi-functional imaging unit. The lower part of the main chassis is integrated with an on-line analysis module and a computer mainframe. The main chassis is a fully enclosed box, which can provide a completely dark condition after closing all the box doors and can be used as a dark adaptation chamber. An installation flange is slidably installed on the lifting shaft, and the multi-functional imaging unit is fixedly installed on the installation flange. The imaging light source includes a full-band light source, a multi-excitation light source, and an ultraviolet light source, and the imaging light source is fixedly installed on the light source support.

[0010] Preferably: The light source support is fixedly installed on the top of the main chassis, the on-line analysis module is placed in the lower part of the main chassis, and the lifting shaft is used for 90° vertical lifting.

[0011] Preferably: An external support is installed outside the main chassis, a computer display screen is placed on the external support, universal wheels are installed at the bottom of the main chassis, and a partition is fixedly installed inside the main chassis.

[0012] Preferably: A network cable is connected to the computer mainframe, and the on-line analysis module is connected to the computer mainframe through the network cable.

[0013] Preferably: The computer mainframe is connected to the multi-functional imaging unit to realize data acquisition work, and a platform control antenna is connected to the computer mainframe to realize remote control of the platform.

[0014] (III) Beneficial effects

[0015] First, the imaging light source of the system is equipped with a variety of excitation light sources, including red light, blue light, green light, etc. Through the hyperspectral multi-functional imaging unit, it realizes the comprehensive analysis function of the multi-excitation light excited chlorophyll fluorescence of the experimental sample in the hyperspectral dimension. Light sources of different wavelengths will excite chlorophyll fluorescence with specific spectral characteristics, and these spectral characteristics not only reflect the chemical state of chlorophyll itself, but also reveal the response mechanism of plants to different light qualities.

[0016] II. The system is equipped with an ultraviolet light source (UV), and through the hyperspectral multi-functional imaging unit, it realizes the comprehensive analysis of the UV-MCF bioluminescence of experimental samples in the hyperspectral dimension. It can simultaneously obtain fluorescence imaging in the blue, green, red, and far-red bands. It can not only perform imaging analysis of bioluminescence such as secondary metabolites (such as polyphenols, flavonoids, ferulic acid, etc.) fluorescence and chlorophyll fluorescence on a two-dimensional scale, but also obtain fluorescence spectral characteristics (spectral fingerprints) and perform fluorescence spectral analysis in the hyperspectral dimension (up to hundreds of them). The combination of the spectrum and the image decodes bioluminescence, providing a more comprehensive perspective and means for the analysis of bioluminescence signals.

[0017] III. The imaging light source of the system is equipped with a multi-channel light source. Through a set of hyperspectral multi-functional imaging units, it can realize the functions of hyperspectral imaging of reflected light of experimental samples, hyperspectral imaging of multi-excitation chlorophyll fluorescence, and hyperspectral imaging analysis of UV-MCF bioluminescence. In this way, it can not only measure traditional hyperspectral imaging of reflected light, but also deeply analyze the physiological and biochemical characteristics such as photosynthesis efficiency, chlorophyll content and its distribution, and bioluminescence signals of plants. Moreover, bioluminescence is autofluorescence and is not affected by external ambient light. The collected data only represents the information specific to the sample. This not only reduces the experimental complexity and cost, but also improves the experimental efficiency and accuracy, enriches the dimension and information volume of experimental data, enhances the flexibility and scalability of the experiment, and thus realizes more refined observation of experimental samples. This multi-modal and multi-scale imaging ability enables this system to be widely applied in multiple fields such as plant physiological ecology, genetics, breeding, pathology, environmental science, postharvest biology, etc., providing strong technical support for scientific research. At the same time, due to the high integration and flexibility of the system, users can freely select and combine different imaging modes according to specific research needs to achieve personalized experimental design and data analysis.

[0018] IV. The system is equipped with an online analysis module. Through the image processing unit and model classification software, based on the powerful spectral recognition ability and flexible classification model, it can output accurate recognition results in real time, simplify the complex coding and spectral image analysis work, and significantly reduce the R & D cost and application threshold for the market application of hyperspectral imaging technology. Description of the Drawings

[0019] The above description is only an overview of the technical solution of the present utility model. In order to be able to more clearly understand the technical means of the present utility model and implement it in accordance with the content of the specification, the following will be described in detail with reference to the preferred embodiments of the present utility model and the accompanying drawings.

[0020] Figure 1 It is the overall structure diagram of the multi-functional hyperspectral imaging analysis system applicable to multiple application fields of the present utility model;

[0021] Figure 2 Structural diagram of the computer mainframe of the present utility model;

[0022] Figure 3 Structural diagram of the imaging light source of the present utility model;

[0023] Figure 4 Structural diagram of the light source bracket of the present utility model.

[0024] Legend: 1. Main chassis; 2. Main control system; 3. Universal wheels; 4. Automatic sample transfer platform; 5. Lifting shaft; 6. Imaging light source; 7. Light source bracket; 8. Multifunctional imaging unit; 9. Mounting flange; 10. Partition; 11. External bracket; 12. Network cable; 13. Antenna; 14. Computer mainframe; 15. Computer display screen; 16. Online analysis module. Detailed implementation manners

[0025] By providing a multifunctional hyperspectral imaging analysis system applicable to multiple application fields in the embodiments of the present application, the problems that chlorophyll fluorescence cannot be analyzed in the spectral dimension, bioluminescence cannot be comprehensively decoded from multiple angles, the multifunctional hyperspectral imaging analysis applicable to multiple application fields that cannot simultaneously take into account reflected light hyperspectrum, multi-excitation light chlorophyll fluorescence hyperspectrum and UV-MCF bioluminescence hyperspectrum imaging, and the results cannot be further analyzed and displayed in real time are effectively solved.

[0026] Embodiment: As Figure 1 , Figure 2 , Figure 3 and Figure 4 shown, the technical solutions in the embodiments of the present application effectively solve the technical problems of the multifunctional hyperspectral imaging analysis applicable to multiple application fields that chlorophyll fluorescence cannot be analyzed in the spectral dimension, bioluminescence cannot be comprehensively decoded from multiple angles, the reflected light hyperspectrum, multi-excitation light chlorophyll fluorescence hyperspectrum and UV-MCF bioluminescence hyperspectrum imaging cannot be simultaneously taken into account, and the results cannot be further analyzed and displayed in real time. The general idea is as follows:

[0027] In view of the problems existing in the prior art, the utility model provides a multifunctional hyperspectral imaging analysis system applicable to multiple application fields, which includes a main chassis 1. The upper part of the main chassis 1 is integrated with a main control system 2, an imaging light source 6, a light source bracket 7, an automatic sample transfer platform 4, a lifting shaft 5, and a multifunctional imaging unit 8. The lower part of the main chassis 1 is integrated with an on-line analysis module 16 and a computer mainframe 14. The main chassis 1 is a fully enclosed box body, and it has completely dark conditions after all the box doors are closed, which can be used as a dark adaptation chamber. An installation flange 9 is slidably installed on the lifting shaft 5, and the multifunctional imaging unit 8 is fixedly installed on the installation flange 9. The imaging light source 6 includes a full-band light source, a multi-excitation light source, and an ultraviolet light source. The imaging light source 6 is fixedly installed on the light source bracket 7, and the light source bracket 7 is fixedly installed on the top of the main chassis 1. The on-line analysis module 16 is placed in the lower part of the main chassis 1. The lifting shaft 5 is used for 90° vertical lifting. An external bracket 11 is installed outside the main chassis 1, and a computer display screen 15 is placed on the external bracket 11. Universal wheels 3 are installed at the bottom of the main chassis 1. A partition 10 is fixedly installed inside the main chassis 1. A network cable 12 is connected to the computer mainframe 14, and the on-line analysis module 16 is connected to the computer mainframe 14 through the network cable 12. The computer mainframe 14 is connected to the multifunctional imaging unit 8 to realize data acquisition work. A platform control antenna 13 is connected to the computer mainframe 14 to realize remote control of the platform. The imaging light source 6 of this system is equipped with a variety of excitation light sources, including red light, blue light, green light, etc. Through the hyperspectral multifunctional imaging unit 8, the comprehensive analysis function of the multi-excitation light excited chlorophyll fluorescence of the experimental sample in the hyperspectral dimension is realized. Light sources of different wavelengths will excite chlorophyll fluorescence with specific spectral characteristics. These spectral characteristics not only reflect the chemical state of chlorophyll itself, but also reveal the response mechanism of plants to different light qualities. The imaging light source 6 of this system is equipped with an ultraviolet light source (UV). Through the hyperspectral multifunctional imaging unit 8, the comprehensive analysis of the UV-MCF bioluminescence of the experimental sample in the hyperspectral dimension is realized. Fluorescence imaging in the blue, green, red, and far-red bands can be obtained simultaneously. It can not only perform imaging analysis on bioluminescence such as secondary metabolites (such as polyphenols, flavonoids, ferulic acid, etc.) fluorescence and chlorophyll fluorescence in two-dimensional scale, but also obtain fluorescence spectral characteristics (spectral fingerprints) and perform fluorescence spectral analysis in the hyperspectral dimension (up to several hundred). The combination of the spectrum and the image decodes bioluminescence, providing a more comprehensive perspective and means for the analysis of bioluminescence signals. The imaging light source 6 of the system is equipped with a multi-channel light source. Through a set of hyperspectral multifunctional imaging unit 8, the functions of reflectance hyperspectral imaging, multi-excitation light chlorophyll fluorescence hyperspectral imaging, and UV-MCF bioluminescence hyperspectral imaging analysis of the experimental sample can be realized. In this way, traditional reflectance hyperspectral imaging can be measured, and the physiological and biochemical characteristics such as photosynthesis efficiency, chlorophyll content and its distribution, and bioluminescence signals of plants can be deeply analyzed. Moreover, bioluminescence is autofluorescence and is not affected by external ambient light. The collected data only represents the information specific to the sample.This not only reduces the experimental complexity and cost, but also improves the experimental efficiency and accuracy. It also enriches the dimensions and information content of experimental data, enhances the flexibility and scalability of the experiment, thus enabling more refined observation of experimental samples. This multi-modal and multi-scale imaging ability allows this system to be widely applied in multiple fields such as plant physiological ecology, genetics, breeding, pathology, environmental science, postharvest biology, etc., providing strong technical support for scientific research. At the same time, due to the high integration and flexibility of the system, users can freely select and combine different imaging modes according to specific research needs to achieve personalized experimental design and data analysis. This system is equipped with an online analysis module 16. The online analysis module 16 is based on computer technology and image processing algorithms and can acquire and process image information, extract valuable data and features from it, and use artificial intelligence algorithms and technologies to realize the automatic transmission of data, information, or substances. Through the image processing unit and model classification software, and based on its powerful spectral recognition ability and flexible classification model, it can output accurate recognition results in real time, simplify the complex coding and spectral image analysis work, and significantly reduce the R & D cost and application threshold for hyperspectral imaging technology to enter the market application.

[0028] Working principle:

[0029] Step 1: Prepare a sample with appropriate height;

[0030] Step 2: Power on the system to complete the automatic initialization of the sample automatic transfer platform 4 and the lifting shaft 5, confirm that all states are normal, and the multi-functional hyperspectral imaging analysis system is working properly;

[0031] Step 3: Connect the computer host 14 to the multi-functional imaging unit 8 to perform data acquisition work. The computer host 14 is connected to the platform control antenna 13 to enable remote control of the platform;

[0032] Step 4: Place the sample on the sample automatic transfer platform 4 and adjust the sample to determine the best imaging position;

[0033] Step 5: By controlling the uniform movement of the sample automatic transfer platform 4, collect the hyperspectral data of the sample, thus realizing the hyperspectral imaging analysis of the plant;

[0034] Step 6: Through the adjustment of the light source and parameters, realize the switching of the acquisition of reflected light hyperspectral, multi-excitation light excited chlorophyll fluorescence hyperspectral, and UV-MCF bioluminescence hyperspectral data, so as to comprehensively analyze the physical and chemical properties, photosynthetic phenotype, biochemical components, and physiological state of the sample from two angles of reflected light and fluorescence;

[0035] Step 7: Through the spectral recognition ability and classification model of the online analysis module 16, the sample is subjected to model domestication and visual recognition, so as to realize the high-throughput flow operation of automatic imaging, online analysis and sorting.

[0036] Finally, it should be noted that: Obviously, the above embodiments are merely examples for clearly illustrating the present invention, rather than limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all the implementation manners here. And the obvious changes or modifications derived therefrom still fall within the protection scope of the present invention.

Claims

1. A multi-functional hyperspectral imaging analysis system applicable to multiple application fields, including a main chassis (1), characterized in that: The upper part of the main chassis (1) is integrated with a main control system (2), an imaging light source (6), a light source bracket (7), an automatic sample transfer platform (4), a lifting shaft (5), and a multi-functional imaging unit (8). The lower part of the main chassis (1) is integrated with an on-line analysis module (16) and a computer mainframe (14). Among them, the main chassis (1) is a fully enclosed box, which can provide completely dark conditions after all the doors are closed and can be used as a dark adaptation chamber. Among them, a mounting flange (9) is slidably installed on the lifting shaft (5), and the multi-functional imaging unit (8) is fixedly installed on the mounting flange (9). The lifting shaft (5) is used for 90° vertical lifting. Among them, the imaging light source (6) includes a full-band light source, a multi-excitation light source, and an ultraviolet light source, and the imaging light source (6) is fixedly installed on the light source bracket (7).

2. The multi-functional hyperspectral imaging analysis system applicable to multiple application fields according to claim 1, wherein: The light source bracket (7) is fixedly installed on the top of the main chassis (1).

3. A multi-functional hyperspectral imaging analysis system applicable to multiple application fields according to claim 2, characterized in that: The on-line analysis module (16) is placed in the lower part of the main chassis (1).

4. The multi-functional hyperspectral imaging analysis system applicable to multiple application fields according to claim 3, wherein: An external bracket (11) is installed outside the main chassis (1), and a computer display screen (15) is placed on the external bracket (11).

5. A multi-functional hyperspectral imaging analysis system applicable to multiple application fields according to claim 4, characterized in that: Universal wheels (3) are installed at the bottom of the main chassis (1), and a partition (10) is fixedly installed inside the main chassis (1).

6. A multi-functional hyperspectral imaging analysis system applicable to multiple application fields according to claim 1, characterized in that: A network cable (12) is connected to the computer mainframe (14), and the on-line analysis module (16) is connected to the computer mainframe (14) through the network cable (12).

7. A multi-functional hyperspectral imaging analysis system applicable to multiple application fields according to claim 6, characterized in that: The computer mainframe (14) is connected to the multi-functional imaging unit (8) to realize data acquisition work. A platform control antenna (13) is connected to the computer mainframe (14) to realize remote control of the platform.

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