Hematopoietic stem cell storage device

Abstract

The utility model relates to the technical field of hematopoietic stem cell storage, and discloses a hematopoietic stem cell storage device which comprises a tank body, a through hole is formed in the top of the tank body, a liquid nitrogen inlet pipe is fixedly connected in the through hole, a rotating assembly is arranged in the tank body, the rotating assembly comprises a sleeve, and the sleeve is sleeved with the through hole. An inserting hole is formed in one end of the sleeve, the end of the liquid nitrogen inlet pipe is rotationally connected to the interior of the inserting hole, multiple sets of discharging holes distributed in an array mode are formed in the side wall of the sleeve, the bottom face of the tank body is fixedly connected with a motor, and the output end of the motor penetrates through and is rotationally connected to the inner wall of the tank body. According to the hematopoietic stem cell storage device, the sleeve is driven by the motor to rotate, so that liquid nitrogen is uniformly discharged from the discharge holes, and uniform distribution of gas is ensured by flowing of the liquid nitrogen and array distribution of the discharge holes, so that each reagent tube can be in contact with the liquid nitrogen, and long-term storage of hematopoietic stem cells is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of hematopoietic stem cell storage, in particular to a hematopoietic stem cell storage device. Background Technique

[0002] Hematopoietic stem cells are a type of cell with repair ability and play an important role in the field of modern medical treatment. They are used for patients who cannot be cured by conventional therapeutic drugs. The storage environment of hematopoietic stem cells requires extremely low temperatures, so hematopoietic stem cells need to be stored in test tubes and placed in a liquid nitrogen tank.

[0003] After retrieval, the Chinese patent publication number: CN211309326U, discloses a storage device for hematopoietic stem cells. The top view section of the clamping plate is set as an arc structure, and the connecting rod fixedly connected to the clamping plate and the mounting block form a telescopic structure. Under the elastic action of the support spring, the clamping plate can position and clamp the test tube, so that the test tube can be stably inserted into the placement groove.

[0004] The position of the above-mentioned disclosed test tube remains unchanged during storage, resulting in some samples being unable to uniformly contact liquid nitrogen, affecting the cooling effect and cell survival rate. Therefore, a hematopoietic stem cell storage device is proposed to solve the above problems. Content of the Utility Model

[0005] In order to make up for the above deficiencies, the utility model provides a hematopoietic stem cell storage device, aiming to improve the problem that the position of the test tube remains unchanged during storage in the prior art, resulting in some samples being unable to uniformly contact liquid nitrogen, affecting the cooling effect and cell survival rate.

[0006] In order to achieve the above purpose, the utility model adopts the following technical scheme: A hematopoietic stem cell storage device, including a tank body, a through hole is opened at the top of the tank body, and a liquid nitrogen inlet pipe is fixedly connected in the through hole. A rotating component is arranged inside the tank body. The rotating component includes a sleeve. One end of the sleeve is provided with a jack, and the end of the liquid nitrogen inlet pipe is rotatably connected inside the jack. A plurality of groups of discharge holes are arranged in an array on the side wall of the sleeve. The bottom surface of the tank body is fixedly connected with a motor, and the output end of the motor penetrates and is rotatably connected to the inner wall of the tank body. The output end of the motor is fixedly connected to the bottom end of the sleeve.

[0007] As a further description of the above technical solution:

[0008] A bearing component is arranged inside the tank body. The bearing component includes a positioning ring. The positioning ring is fixedly connected to the inner wall of the tank body. A placement plate is attached to the top of the positioning ring. A plurality of groups of reagent tube inlet holes are arranged in an array on the surface of the placement plate.

[0009] As a further description of the above technical solution:

[0010] A fixing ring is fixedly connected to the bottom surface of the placement plate, and the fixing ring is rotatably connected inside the positioning ring.

[0011] As a further description of the above technical solution:

[0012] A plurality of groups of support plates distributed in an array are fixedly connected to the inner wall of the fixing ring, and the support plates are directly above the reagent tube inlet holes.

[0013] As a further description of the above technical solution:

[0014] Support columns are fixedly connected to the surface of the placement plate, and a circular ring is fixedly connected to the top ends of the support columns.

[0015] As a further description of the above technical solution:

[0016] A convex ring is fixedly connected to the surface of the support plate.

[0017] As a further description of the above technical solution:

[0018] An inlet and outlet is provided on the side wall of the tank body, and a sealing door is hinged to the side wall of the tank body.

[0019] As a further description of the above technical solution:

[0020] Four symmetrically arranged support legs are fixedly connected to the bottom surface of the tank body.

[0021] The present utility model has the following beneficial effects:

[0022] 1. In the present utility model, liquid nitrogen enters the tank body through the sleeve of the rotating assembly, and the sleeve rotates driven by the motor, so that the liquid nitrogen is evenly discharged from the discharge holes, thereby forming a uniform low-temperature environment inside the tank body. The flow of liquid nitrogen and the array distribution of the discharge holes ensure the uniform distribution of gas, so that each reagent tube can come into contact with liquid nitrogen, ensuring the long-term preservation of hematopoietic stem cells.

[0023] 2. In the present utility model, the reagent tube is placed on the placement plate through the reagent tube inlet hole, and the convex ring ensures the fixed position of the reagent tube on the placement plate, preventing the reagent tube from shifting or toppling in liquid nitrogen. The setting of the support columns and the circular ring enables the operator to drive the placement plate to rotate by rotating the circular ring, thereby facilitating the access of the reagent tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of a hematopoietic stem cell storage device proposed by the present utility model;

[0025] Figure 2Schematic cross-sectional structure diagram of a hematopoietic stem cell storage device proposed by the present utility model;

[0026] Figure 3 Schematic structure diagram of a rotating assembly of a hematopoietic stem cell storage device proposed by the present utility model;

[0027] Figure 4 Schematic structure diagram of a bearing assembly of a hematopoietic stem cell storage device proposed by the present utility model.

[0028] Legend:

[0029] 1. Tank body; 11. Inlet and outlet; 12. Sealing door; 13. Support legs; 2. Liquid nitrogen inlet pipe; 3. Rotating assembly; 31. Sleeve; 32. Jack; 33. Discharge hole; 34. Motor; 4. Bearing assembly; 41. Positioning ring; 42. Placing plate; 43. Reagent tube inlet hole; 44. Fixed ring; 45. Support plate; 451. Convex ring; 46. Support column; 47. Ring. Specific implementation manners

[0030] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present utility model.

[0031] Refer to Figure 1 - Figure 2 As shown in FIG. - FIG., an embodiment provided by the present utility model: A hematopoietic stem cell storage device includes a tank body 1. The tank body 1 is used to store liquid nitrogen and reagent tubes containing hematopoietic stem cells. An inlet and outlet 11 is provided on the side wall of the tank body 1. A sealing door 12 is hinged on the side wall of the tank body 1. The inlet and outlet 11 is used to access the reagent tubes of hematopoietic stem cells, and the sealing door 12 is used to close the inlet and outlet 11. Four groups of symmetrically arranged support legs 13 are fixedly connected to the bottom surface of the tank body 1. The support legs 13 are used to stabilize the tank body 1 and prevent it from tilting or shaking during use. A through hole is provided at the top of the tank body 1, and a liquid nitrogen inlet pipe 2 is fixedly connected in the through hole. The liquid nitrogen inlet pipe 2 introduces liquid nitrogen into the interior of the tank body 1.

[0032] Refer to Figure 2 - Figure 3, a rotating assembly 3 is provided inside the tank body 1. The rotating assembly 3 helps to evenly distribute liquid nitrogen and promotes gas flow, preventing some samples from being unable to contact liquid nitrogen evenly and affecting the cooling effect and cell survival rate. The rotating assembly 3 includes a sleeve 31. One end of the sleeve 31 is provided with an insertion hole 32. The end of the liquid nitrogen inlet pipe 2 is rotatably connected inside the insertion hole 32. The sleeve 31 is used to connect the liquid nitrogen inlet pipe 2 to ensure the smooth inflow of liquid nitrogen and is driven to rotate by a motor 34. The side wall of the sleeve 31 is provided with multiple groups of discharge holes 33 distributed in an array. The discharge holes 33 are used for the discharge of liquid nitrogen and the even distribution of gas. The bottom surface of the tank body 1 is fixedly connected to the motor 34. The output end of the motor 34 penetrates and is rotatably connected to the inner wall of the tank body 1. The output end of the motor 34 is fixedly connected to the bottom end of the sleeve 31. The motor 34 is used to drive the rotating assembly 3 to rotate the sleeve 31 to assist in the distribution of liquid nitrogen and the flow of gas.

[0033] Refer to Figure 2 , Figure 4 , a carrying assembly 4 is provided inside the tank body 1. The carrying assembly 4 is used to carry and support reagent tubes to ensure their correct positions in liquid nitrogen. The carrying assembly 4 includes a positioning ring 41. The positioning ring 41 is fixedly connected to the inner wall of the tank body 1. A placement plate 42 is attached to the top of the positioning ring 41. The surface of the placement plate 42 is provided with multiple groups of reagent tube inlet holes 43 distributed in an array. The positioning ring 41 is used to support the placement plate 42. The reagent tube inlet holes 43 are used for the entry of reagent tubes. The bottom surface of the placement plate 42 is fixedly connected to a fixing ring 44. The fixing ring 44 is rotatably connected inside the positioning ring 41. The inner wall of the fixing ring 44 is fixedly connected with multiple groups of support plates 45 distributed in an array. The surface of the support plate 45 is fixedly connected with a convex ring 451. The support plate 45 is directly above the reagent tube inlet hole 43. The fixing ring 44 is used to position the placement plate 42 to ensure the stability of its position during rotation and support the support plate 45. The bottom end of the reagent tube is supported by the support plate 45, and the position of the reagent tube is fixed by the convex ring 451. A support column 46 is fixedly connected to the surface of the placement plate 42. The top end of the support column 46 is fixedly connected to a circular ring 47. The support column 46 is used to fixedly connect the circular ring 47 and the support column 46 together. By rotating the circular ring 47, the placement plate 42 is driven to rotate, facilitating the access of reagent tubes.

[0034] Working principle: When the liquid nitrogen inlet pipe 2 introduces liquid nitrogen into the inside of the tank body 1, the liquid nitrogen first enters the tank body through the sleeve 31 of the rotating assembly 3. The sleeve 31 rotates under the drive of the motor 34, causing the liquid nitrogen to be evenly discharged from the discharge holes 33, thereby forming a uniform low-temperature environment inside the tank body 1. The flow of liquid nitrogen and the array distribution of the discharge holes 33 ensure the even distribution of gas, enabling each reagent tube to contact liquid nitrogen and guaranteeing the long-term preservation of hematopoietic stem cells.

[0035] The bearing component 4 stably bears the reagent tube in a liquid nitrogen environment. The positioning ring 41 is fixed on the inner wall of the tank body 1 to ensure the stable position of the placement plate 42. The reagent tube is placed on the placement plate 42 through the reagent tube access hole 43. The rotation of the fixing ring 44 enables the placement plate 42 to rotate, facilitating the operator to access the reagent tube. The support plate 45 and the convex ring 451 ensure the fixed position of the reagent tube on the placement plate 42, preventing the reagent tube from shifting or toppling in liquid nitrogen. The setting of the support column 46 and the circular ring 47 enables the operator to drive the placement plate 42 to rotate by rotating the circular ring 47, thereby conveniently accessing the reagent tube.

[0036] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A hematopoietic stem cell storage device, comprising a tank body (1), characterized in that: A through hole is formed at the top of the tank body (1), and a liquid nitrogen inlet pipe (2) is fixedly connected in the through hole. A rotating assembly (3) is arranged inside the tank body (1). The rotating assembly (3) includes a sleeve (31). One end of the sleeve (31) is provided with a jack (32). The end of the liquid nitrogen inlet pipe (2) is rotatably connected inside the jack (32). A plurality of groups of discharge holes (33) distributed in an array are formed in the side wall of the sleeve (31). The bottom surface of the tank body (1) is fixedly connected to a motor (34). The output end of the motor (34) penetrates and is rotatably connected to the inner wall of the tank body (1). The output end of the motor (34) is fixedly connected to the bottom end of the sleeve (31).

2. The hematopoietic stem cell storage device according to claim 1, wherein: A bearing assembly (4) is arranged inside the tank body (1). The bearing assembly (4) includes a positioning ring (41). The positioning ring (41) is fixedly connected to the inner wall of the tank body (1). A placement plate (42) is attached to the top of the positioning ring (41). A plurality of reagent tube inlet holes (43) distributed in an array are formed on the surface of the placement plate (42).

3. The hematopoietic stem cell storage device according to claim 2, characterized in that: A fixing ring (44) is fixedly connected to the bottom surface of the placement plate (42). The fixing ring (44) is rotatably connected inside the positioning ring (41).

4. The hematopoietic stem cell storage device according to claim 3, wherein: A plurality of support plates (45) distributed in an array are fixedly connected to the inner wall of the fixing ring (44). The support plates (45) are located directly above the reagent tube inlet holes (43).

5. The hematopoietic stem cell storage device according to claim 2, characterized in that: A support column (46) is fixedly connected to the surface of the placement plate (42). The top end of the support column (46) is fixedly connected to a ring (47).

6. The hematopoietic stem cell storage device according to claim 4, wherein: A convex ring (451) is fixedly connected to the surface of the support plate (45).

7. The hematopoietic stem cell storage device according to claim 1, characterized in that: An inlet and outlet (11) is formed in the side wall of the tank body (1). A sealing door (12) is hinged to the side wall of the tank body (1).

8. A hematopoietic stem cell storage device according to claim 1, characterized in that: Four symmetrically arranged support legs (13) are fixedly connected to the bottom surface of the tank body (1).

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