Swing mixing device

Abstract

The utility model relates to uniform mixing equipment, in particular to a swinging uniform mixing device. A swing uniform mixing device comprises a base for installation, a swing mechanism for uniform shaking, a magnetic bead adsorption mechanism and a liquid beating and discharging mechanism are installed on the base, and the magnetic bead adsorption mechanism can move up and down; the swinging mechanism comprises two support frames fixed with the base, the top ends of the support frames are movably provided with swinging shafts for mounting test tubes through bearings, the outer side of one of the support frames is fixedly provided with a motor frame, the motor frame is provided with a first motor, and the first motor is fixedly connected with the swinging shafts. According to the swing mixing device, the test tube filled with the magnetic beads is placed in the swing mechanism, the swing mechanism simulates a manual swing mode of a person, a reagent and the magnetic beads are fully reacted, and then the magnetic beads are rapidly condensed by the magnetic bead adsorption mechanism, so that the reagent is convenient to extract.

Description

Technical Field

[0001] The utility model relates to a mixing device, in particular to a swing mixing device. Background Art

[0002] Magnetic beads are commonly used raw materials in the fields of medical diagnosis and biological detection. Magnetic beads, also known as biological magnetic beads, refer to superparamagnetic microspheres with small particle sizes. They can quickly aggregate in a magnetic field and disperse evenly after leaving the magnetic field. They generally have appropriate and relatively small particle size differences, ensuring strong enough magnetic responsiveness without sedimentation. Biological magnetic beads usually have rich surface active groups so that they can be coupled with biochemical substances and separated from the samples to be tested under the action of an external magnetic field. Compared with traditional separation methods, using magnetic beads for the separation of complex components in biochemical samples can achieve separation and enrichment simultaneously, effectively improving the separation speed and enrichment efficiency, and also greatly enhancing the sensitivity of analysis and detection. By coating specific antibodies, receptors, etc. on the surface of magnetic beads, they can be used to separate and purify the targets in the samples. Magnetic beads have been widely used in many fields such as immunoassay, nucleic acid separation and extraction, cell sorting, enzyme immobilization, separation of bioactive substances, and food safety detection. Especially in in vitro diagnostic immunoassays, it is usually necessary to mix the reaction system to make the reaction more complete. In the prior art, the mixing techniques mainly include mixing rod mixing and ultrasonic mixing. However, mixing rod mixing is prone to the risk of cross-infection, and the structure of ultrasonic mixing is complex and expensive, thus restricting the popularization and application of reagent mixing techniques.

[0003] A Chinese patent document (application number: 201811094501.5) discloses a magnetic bead chemiluminescence detection kit, which includes a box body, a box cover, reagent bottles, a magnetic bead bottle, and a bracket; the lower box body includes an upper box body and a lower box body; the upper box body and the lower box body are not connected; the box cover is hinged to the upper box body; the lower box body is a push-pull drawer; a gasket made of rigid plastic foam is provided in the upper box body; bottle grooves are provided on the gasket; the bottle grooves include a magnetic bead bottle groove and a plurality of reagent bottle grooves; the size of the magnetic bead bottle groove is adapted to the magnetic bead bottle, and the size of the reagent bottle groove is adapted to the reagent bottle; snap structures adapted to each other are provided between the reagent bottles and the gasket, and between the magnetic bead bottle and the gasket; a limiting mechanism for preventing the magnetic bead bottle from axially moving in the magnetic bead bottle chamber is provided between the magnetic bead bottle and the bracket; tooth patterns are provided at the lower part of the magnetic bead bottle; through holes are provided at the bottom of the magnetic bead bottle chamber; after the magnetic bead bottle is placed in the magnetic bead bottle chamber, the part with tooth patterns extends out of the magnetic bead bottle chamber through the through holes; the magnetic bead bottle can rotate freely in the magnetic bead bottle chamber; the lower box body is separated by a partition into a bracket storage area and an operation tool storage area; a sponge gasket is provided in the bracket storage area, the size of the sponge gasket is adapted to the bracket storage area, and bracket grooves are provided on the sponge gasket. However, the structure and method for detecting using magnetic beads in this invention are complex.

[0004] Chinese patent document (application number: 201820280791.1) discloses a magnetic bead mixing component and a magnetic bead mixing mechanism. The magnetic bead mixing component mechanism includes a driving mechanism and a supporting mechanism. A plurality of limiting rings are arranged on the supporting mechanism, and a plurality of limiting rings are arranged on the supporting mechanism at intervals; each limiting ring is connected to the driving mechanism by transmission. The magnetic bead bottle can be sleeved in the limiting ring. The limiting ring is driven to rotate by the driving mechanism, so that the magnetic bead bottle placed inside the limiting ring can be driven to rotate, and then the magnetic beads in the magnetic bead bottle are mixed. When the magnetic bead mixing component is mixed, the limiting ring rotates stably, without noise, and the mixing effect is good. By setting the limiting ring, the magnetic bead bottle can be directly placed in each limiting ring, which is very convenient to put in and take out, and greatly facilitates the operation compared with the prior art. This technical solution does not disclose a method for stirring and mixing magnetic beads, and discloses a magnetic bead stirring and mixing component with a more complex structure.

[0005] In view of the above-mentioned defects, the designers have actively carried out research and innovation in order to create a swing mixing device to make it more valuable for industrial use. Utility Model Content

[0006] In order to solve the above technical problems, the purpose of the utility model is to provide a rocking mixing device.

[0007] The utility model discloses a swing mixing device, comprising a base for installation, on which a swing mechanism for shaking, a magnetic bead adsorption mechanism and a liquid discharging mechanism are installed, and the magnetic bead adsorption mechanism can move up and down;

[0008] The swing mechanism includes two support frames fixed to the base, the top of the support frame is movably mounted with a swing shaft for mounting the test tube through a bearing, a motor frame is fixed on the outside of one of the support frames, a first motor is mounted on the motor frame, and the first motor is fixedly connected to the swing shaft.

[0009] This rocking and mixing device places the test tube containing magnetic beads in a rocking mechanism, which simulates manual rocking to allow the reagent and magnetic beads to react fully, and then the magnetic bead adsorption mechanism quickly condenses the magnetic beads to facilitate the extraction of the reagent.

[0010] Furthermore, a test tube slot for placing test tubes is provided in the middle of the swing shaft, and an electric heating block is fixed below the swing shaft, and the electric heating block wraps the test tube slot.

[0011] The electric heating block installed under the swing shaft can assist in heating, facilitating constant temperature reaction of the reagents.

[0012] Furthermore, the magnetic bead adsorption mechanism includes two electromagnetic rings that can wrap around the protrusion at the lower end of the electric heating block.

[0013] The magnetic bead adsorption mechanism provides magnetic force to the magnetic beads in the test tube through the electromagnetic ring, which facilitates the aggregation of the magnetic beads.

[0014] Furthermore, the connecting plate at the lower end of the magnetic bead adsorption mechanism is fixedly connected to the lifting plate, a first cross arm is movably installed on one side of the lower end of the lifting plate through an axis, a first slide groove is provided at the other end of the lifting plate, a first slider is installed in the first slide groove, a second cross arm is movably installed on the first slider through an axis, the other end of the second cross arm is movably connected to the support plate through the axis, a second slide groove is provided on the support plate, a second slider is installed in the second slide groove, the second slider is movably connected to the other end of the first cross arm through the axis, a screw is movably installed in the second slide groove through a bearing, a screw hole matching the screw is provided on the second slider, the tail end of the screw is fixedly connected to the second motor, the support plate and the second motor are fixedly installed on the base, and the middle parts of the first cross arm and the second cross arm are movably connected through the axis.

[0015] The magnetic bead adsorption mechanism moves up and down with the lifting plate, and moves along the test tube slot on the swing shaft when the swing shaft shakes. After the shaking is completed, it docks with the test tube slot to facilitate the aggregation of magnetic beads in the test tube and the separation of reagents.

[0016] Furthermore, the liquid injection and discharge mechanism includes a mounting plate that can move forward and backward, a slide cylinder is fixed on the mounting plate, and a liquid injection needle rack with the same number of liquid injection needles as the test tube slots is installed on the slider of the slide cylinder.

[0017] The liquid injection and discharge mechanism is a movable structure, which is far away when the swing shaft is running, so as to facilitate swinging, and the reagent can be injected and discharged when it is stationary.

[0018] Furthermore, a sliding support plate is fixed on the base, and two groups of parallel guide rods are installed on the sliding support plate through a bracket, the sliding block is sleeved on the guide rod, an adapter plate is fixed above the sliding block, the front end of the adapter plate is fixedly connected to the mounting plate, an intermediate plate is fixed between the two adapter plates by bolts, a screw sleeve is fixed under the intermediate plate, and a third motor and a screw support seat are fixed to the tail end of the sliding support plate through the bracket, the screw is installed on the screw support seat through a bearing, the screw cooperates with the screw sleeve, and the tail end is fixedly connected to the third motor.

[0019] The movement of the mounting plate is driven by the third motor, which can drive the corresponding injection needle to approach or move away from the swing axis, so as to facilitate the mixing, injection and discharge operations of the reagents.

[0020] Through the above scheme, the present invention has at least the following advantages: this rocking mixing device fully mixes the magnetic beads and reagents by simulating manual shaking. After the mixing is completed, the magnetic bead adsorption mechanism is magnetized to aggregate the magnetic beads in the test tube, which facilitates the removal of the reagents and improves the success rate and efficiency of the reaction.

[0021] The above description is only an overview of the technical solution of the present utility model. In order to better understand the technical means of the present utility model and be able to implement it according to the content of the description, the following will be a detailed description of the preferred embodiments of the present invention in conjunction with the accompanying drawings as follows. Description of the Drawings

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the accompanying drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present utility model and should not be regarded as limiting the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings.

[0023] Figure 1 is a schematic structural diagram of the present utility model;

[0024] Figure 2 is a schematic structural diagram of the swing mechanism of the present utility model;

[0025] Figure 3 is the present utility model Figure 2 schematic diagram of another perspective;

[0026] Figure 4 is a schematic structural diagram of the present utility model for driving the magnetic bead adsorption mechanism to move up and down;

[0027] Figure 5 is the present utility model Figure 4 schematic diagram of another perspective;

[0028] Figure 6 is the present utility model Figure 1 schematic diagram of an oblique rear perspective.

[0029] In the figure, 1 is the base, 2 is the swing mechanism, 3 is the magnetic bead adsorption mechanism, 4 is the liquid injection and drainage mechanism, 5 is the support frame, 6 is the swing shaft, 7 is the motor frame, 8 is the first motor, 9 is the electric heating block, 10 is the test tube slot, 11 is the electromagnetic ring, 12 is the lifting plate, 13 is the first cross arm, 14 is the first chute, 15 is the first slider, 16 is the second cross arm, 17 is the support plate, 18 is the second chute, 19 is the second slider, 20 is the second motor, 21 is the mounting plate, 22 is the slide cylinder, 23 is the liquid injection needle holder, 24 is the liquid injection needle, 25 is the sliding support plate, 26 is the guide rod, 27 is the sliding block, 28 is the adapter plate, 29 is the intermediate plate, 30 is the third motor, and 31 is the screw support seat. Detailed Embodiments

[0030] The following will further describe in detail the specific embodiments of the present utility model in conjunction with the drawings and embodiments. The following embodiments are used to illustrate the present utility model but are not used to limit the scope of the present utility model.

[0031] See Figures 1-3 In this kind of swing mixing device, the reagent tube is placed on the swing shaft 6. At this time, the magnetic bead adsorption mechanism 3 and the liquid injection and drainage mechanism 4 are far away from the swing shaft 6. The swing shaft 6 starts to swing left and right under the drive of the first motor 9, simulating the manual swing method, and reacting the magnetic beads evenly with the reagent.

[0032] The swing shaft 6 is installed on two support frames 5 through bearings. The first motor 8 is a reciprocating motor, which drives the swing shaft 6 to swing left and right.

[0033] See Figure 2 and Figure 3 In, there is a test tube slot 10 in the middle of the swing shaft 6. The test tube with magnetic beads and reagent is inserted into the test tube slot 10 to complete the placement. There is a detachable cover plate above the test tube slot 10, and the cover plate can limit the reagent tube. The electric heating block 9 at the lower end of the swing shaft 6 serves the purpose of auxiliary heating, so that the reagent can be kept warm during the preparation process, improving the efficiency of reagent preparation.

[0034] Refer to Figure 1 and Figure 4 After being electrified, the electromagnetic ring 11 forms a magnetic field, gathering the magnetic beads located inside the heating block 9, facilitating the removal of the reagent.

[0035] Refer to Figure 4 and Figure 5 In, there is a movable connection between the lifting plate 12 of the magnetic bead adsorption mechanism 3 and the support plate 17 at the bottom through the first cross arm 13 and the second cross arm 16 installed crosswise. When the second motor 20 drives the screw to rotate, the second slider 19 at the end of the first cross arm 13 moves back and forth. Since one end of the first cross arm 13 and the second cross arm 16 are respectively movably connected to the ends of the lifting plate 12 and the support plate 17, and the other end is movably connected to the first slider 15 and the second slider 19, after the second slider 19 is driven, the included angle between the first cross arm 13 and the second cross arm 16 can be changed, thereby completing the operation of driving the lifting plate 12 to lift and lower.

[0036] See Figure 1 and Figure 6 In, the liquid injection and drainage mechanism 4 is fixed on the mounting plate 21. This mounting plate 21 can move back and forth. The slide table cylinder 22 on the mounting plate 21 is used to drive the liquid injection needle holder 23 equipped with the liquid injection needle 24 to move up and down, so that the liquid injection needle 24 enters the test tube slot 10, facilitating the liquid injection and liquid extraction operations.

[0037] Refer to Figure 6, two sets of parallel guide rods 26 are fixed on the sliding support plate 25 on the base 1. Two sliding blocks 27 are sleeved on the corresponding guide rods 26 respectively. When the third motor 30 rotates, the screw rod rotates. The nut sleeve fixed on the middle plate 29 cooperates with the screw rod, which can drive the sliding block 27 to move back and forth. The movement of the sliding block 27 drives the mounting plate 21 fixed on the adapter plate 28 to move, so as to drive the liquid injection and drainage mechanism 4 to move.

[0038] The working principle of the present utility model is as follows:

[0039] When using this swing mixing device, first place the test tube into the test tube slot 10 of the swing shaft 6, put magnetic beads into the test tube slot 10, and then start the third motor 30. Driven by the third motor 30, the liquid injection and drainage mechanism 4 installed on the mounting plate 21 moves to directly above the swing shaft 6. Start the slide cylinder 22 to drive the liquid injection needle holder 23 to move downward, so that the liquid injection needle 24 extends into the test tube. First, inject the reagent, and then the slide cylinder 22 resets. The third motor 30 drives the mounting plate 21 to reset. Start the first motor 8 to drive the swing shaft 6 to swing left and right, simulating the manual shaking method. At the same time, the electric heating block 9 also keeps the test tube slot 10 warm to improve the reaction efficiency.

[0040] After the reagent reaction is completed, start the second motor 20 to move the magnetic bead adsorption mechanism 3 upward. The electromagnetic ring 11 at its upper end is sleeved on the outer wall of the test tube slot 10. Start the electromagnetic ring 11 to make the magnetic beads obtain magnetism and gather together. At this time, start the third motor 30 and the slide cylinder 22 to extend the liquid injection needle 24 into the test tube to suck the reagent after the reaction. After the reagent is sucked, inject the reagent again and repeat the operation.

[0041] Finally, several points should be noted: First, in the description of the present application, it should be noted that unless otherwise specified and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense, which can be mechanical connection or electrical connection, or the communication inside two components, and can be directly connected. "Up", "down", "left", "right", etc. are only used to represent the relative position relationship. When the absolute position of the described object changes, the relative position relationship may change;

[0042] Secondly: In the attached drawings of the disclosed embodiments of the present utility model, only the structures related to the disclosed embodiments are involved. Other structures can refer to the general design. Without conflict, the same embodiment and different embodiments of the present utility model can be combined with each other;

[0043] Finally, the above description is only the preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that for those of ordinary skill in the art, without departing from the technical principle of the present invention, several improvements and modifications can still be made, and these improvements and modifications should also be regarded as the protection scope of the present utility model.

Claims

1. A swinging and mixing device, comprising a base (1) for installation, characterized in that: A swinging mechanism (2) for shaking, a magnetic bead adsorption mechanism (3), and a liquid injection and drainage mechanism (4) are installed on the base (1). The magnetic bead adsorption mechanism (3) can move up and down. The swinging mechanism (2) includes two support frames (5) fixed to the base (1). The top of the support frame (5) is movably installed with a swinging shaft (6) for installing a test tube through a bearing. A motor frame (7) is fixed outside one of the support frames (5). A first motor (8) is installed on the motor frame (7), and the first motor (8) is fixedly connected to the swinging shaft (6).

2. The swing mixing device according to claim 1, characterized in that: There is a test tube slot (10) for placing a test tube in the middle of the swinging shaft (6). An electric heating block (9) is fixed below the swinging shaft (6), and the electric heating block (9) wraps the test tube slot (10).

3. The swing mixing device according to claim 2, characterized in that: The magnetic bead adsorption mechanism (3) includes two electromagnetic rings (11) that can wrap the protrusions at the lower end of the electric heating block (9).

4. The swing mixing device according to claim 3, characterized in that: The connecting plate at the lower end of the magnetic bead adsorption mechanism (3) is fixedly connected to the lifting plate (12). One side of the lower end of the lifting plate (12) is movably installed with a first cross arm (13) through a shaft. There is a first sliding slot (14) at the other end of the lifting plate (12). A first sliding block (15) is installed in the first sliding slot (14). A second cross arm (16) is movably installed on the first sliding block (15) through a shaft. The other end of the second cross arm (16) is movably connected to a support plate (17) through a shaft. There is a second sliding slot (18) on the support plate (17). A second sliding block (19) is installed in the second sliding slot (18). The other end of the second sliding block (19) is movably connected to the other end of the first cross arm (13) through a shaft. A screw rod is movably installed in the second sliding slot (18) through a bearing. There is a threaded hole on the second sliding block (19) that cooperates with the screw rod. The tail end of the screw rod is fixedly connected to a second motor (20). The support plate (17) and the second motor (20) are fixedly installed on the base (1). The middle parts of the first cross arm (13) and the second cross arm (16) are movably connected through a shaft.

5. The swing mixing device according to claim 4, wherein: The liquid injection and drainage mechanism (4) includes a mounting plate (21) that can move back and forth. A slide cylinder (22) is fixed on the mounting plate (21). A liquid injection needle holder (23) and liquid injection needles (24) with the same number as the test tube slots (10) are installed on the slider of the slide cylinder (22).

6. The swing mixing device according to claim 5, characterized in that: A sliding support plate (25) is fixed on the base (1). Two groups of parallel guide rods (26) are installed on the sliding support plate (25) through brackets. A sliding block (27) is sleeved on the guide rods (26). A transfer plate (28) is fixed above the sliding block (27). The front end of the transfer plate (28) is fixedly connected to the mounting plate (21). An intermediate plate (29) is fixed between the two transfer plates (28) through bolts. A screw sleeve is fixed below the intermediate plate (29). The tail end of the sliding support plate (25) is fixed with a third motor (30) and a screw rod support seat (31) through brackets. The screw rod is installed on the screw rod support seat (31) through a bearing. The screw rod cooperates with the screw sleeve and is fixedly connected to the third motor (30) at the tail end.

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