Automatic optical information acquisition device for microbial culture
Through a non-interventional optical information acquisition device combining a camera and an ultraviolet visible light source, the error and pollution problems of optical information acquisition during microbial culture are solved, and efficient and accurate automatic collection of optical information is achieved.
Patent Information
- Application Number
- CN202422218772.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-10
- Publication Date
- 2025-07-18
- Estimated Expiration
- 2034-09-10
AI Technical Summary
In the existing microbial culture process, optical information collection methods are prone to introduce artificial errors and increase costs, and there is a risk of pollution.
A non-interventional optical information acquisition device combining a camera and an ultraviolet visible light source is used to automatically collect RGB color information, resolution, grayscale and sharpness of the culture system by referring to the design of the plate and culture carrier.
It reduces artificial operation errors, reduces the risk of experimental pollution, and improves the efficiency and accuracy of optical information collection.
Smart Images
Figure CN223118468U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of microorganism culture detection, and particularly relates to an optical information automatic acquisition device for microorganism culture. Background Art
[0002] Microorganism culture refers to the process of enabling certain (species) microorganisms to grow and reproduce rapidly by means of artificially prepared culture media and artificially created culture conditions (such as temperature, humidity, light, oxygen concentration, etc.), and it is an important part of the field of life sciences. During the microorganism culture process, it is often necessary to collect the optical information of the culture system during the culture process, and then analyze and calculate it through a computer to finally obtain various physiological and biochemical information during the microorganism culture process.
[0003] The currently commonly used method for collecting optical information in microorganism culture is to use a UV-visible spectrophotometer to sample the culture system at regular intervals under certain conditions, and then use the UV-visible spectrophotometer to detect the taken solution sample, and then analyze and calculate its optical information. This method relies on personnel for sampling and detection, and it is easy to cause experimental errors due to improper operation of personnel, and sometimes it will contaminate the culture system.
[0004] In addition, another detection method is to directly connect an optical fiber to a UV-visible light source and a UV-visible light spectrometer, and the probe is connected to the culture system to obtain optical information, which is convenient for subsequent analysis and calculation. This device greatly increases the cost due to the need for a relatively expensive UV-visible light spectrometer; at the same time, since it needs to be inserted into the culture system, there is a risk of contamination. Content of the Utility Model
[0005] Aiming at the deficiencies existing in the prior art, the utility model provides an optical information automatic acquisition device for microorganism culture, which realizes non-invasive and rapid acquisition of the optical information of the microorganism culture system.
[0006] The utility model is realized through the following technical solutions:
[0007] An optical information automatic acquisition device for microorganism culture includes a first UV-visible light source, a carrier plate and a camera;
[0008] A reference plate is fixed above the carrier plate. The carrier plate is made of a transparent material. A culture carrier is placed on the upper surface of the carrier plate. The culture carrier carries a culture system for which optical information is to be obtained. The optical information includes RGB color information, resolution, grayscale and sharpness. There is a gap between the lower surface of the reference plate and the opening of the culture carrier. Patterns and lines for extracting RGB color information, resolution, grayscale and sharpness are printed on the lower surface of the reference plate;
[0009] The first ultraviolet and visible light source is arranged on the upper surface of the reference plate, and the light-emitting surface of the first ultraviolet and visible light source is located on its lower surface. The camera is arranged on the lower surface of the carrier plate, and a plurality of second ultraviolet and visible light sources are arranged around the lens of the camera. All the second ultraviolet and visible light sources are located directly below the corresponding positions on the lower surface of the culture carrier.
[0010] Preferably, the culture carrier is an Erlenmeyer flask, a beaker, a flat-bottomed flask or a petri dish.
[0011] Preferably, the first ultraviolet and visible light source is placed on the upper surface of the reference plate, and the edge of the first ultraviolet and visible light source is close to the corresponding edge of the reference plate.
[0012] Furthermore, the opening of the culture carrier is located directly below the center of the lower surface of the reference plate.
[0013] Preferably, the vertical distance between the opening of the culture carrier and the lower surface of the reference plate is 2-4 cm.
[0014] Preferably, the edge of the carrier plate is flush with the edge of the lower surface of the culture carrier, and the center of the lower surface of the carrier plate is placed on the lens of the camera.
[0015] Preferably, the camera includes a fixed seat with a square cross-section and a lens arranged at the center of the fixed seat. The lens is a ring structure, and the edge of the lens is flush with the edge of the fixed seat. There are four second ultraviolet and visible light sources, which are respectively distributed at the four corners of the fixed seat, and there is a gap between each second ultraviolet and visible light source and the outer edge of the lens.
[0016] Furthermore, the lens is located at the center of the lower surface of the culture carrier.
[0017] Preferably, it further includes two vertically distributed columns arranged on both sides of the culture carrier. The reference plate and the carrier plate are both square and have the same side length. The center of the reference plate is directly above the center of the carrier plate. The lower end of each column is fixed at a corner of the carrier plate, and the upper end of each column is fixed at the corresponding corner of the reference plate. The lower ends of the two columns are distributed along the diagonal direction of the carrier plate.
[0018] Further, it further includes four reinforcing plates that are horizontally distributed and have the same length. The length of the reinforcing plates is the side length of the reference plate. The four reinforcing plates form a square protective frame body. The upper end of the protective frame body is perpendicular to the lower surface of the reference plate. The edge of each reinforcing plate is flush with the edge of the corresponding side of the lower surface of the reference plate. The cross-section of the upright column is circular, and the diameters of the two upright columns are the same. A first notch with an inner wall fitting the outer wall of one upright column is provided at the joint of two adjacent reinforcing plates, and a second notch with an inner wall fitting the outer wall of the other upright column is provided at the joint of the other two adjacent reinforcing plates. One upright column is in contact with the first notch, and the other upright column is in contact with the second notch.
[0019] Compared with the prior art, the utility model has the following beneficial technical effects:
[0020] An optical information automatic acquisition device for microorganism culture of the utility model. The first ultraviolet-visible light source can illuminate the reference plate through its own light-emitting surface. When it is necessary to acquire the optical information of the culture system, the camera receives the light from the reference plate through the transparent carrier plate and the culture carrier. Through the specific lines and standard color block patterns printed on the top reference plate, the camera can take pictures of the image on the reference plate to obtain the RGB color information, resolution, grayscale, and sharpness of the culture system. The RGB color information is convenient for further spectral information analysis as needed. When it is necessary to obtain the absorbance curve, the second ultraviolet-visible light source can illuminate the culture system in the culture carrier. The camera takes pictures of the culture system through the transparent carrier plate and the culture carrier to obtain the RGB color information of the culture system, which can be further converted into the absorbance value at a certain wavelength, so as to obtain the absorbance curve. It can also measure the turbidity of the culture system through the grayscale and sharpness, and judge whether there are flocculent or other forms of solids in the system through the resolution and the uniformity of the culture system. The ultraviolet-visible light source can take pictures at different times. The turbidity of the culture system can be judged by the clarity of the specific lines on the photo, and the specific absorbance parameters of the liquid can be obtained by converting the RGB color information obtained from the standard color block pattern. The ultraviolet-visible light source has the ability to emit multiple different wavelength lights in the corresponding wavelength band. The camera can collect the specific photos of the culture system under different frequency lights by taking pictures under different wavelength lights, and obtain the absorbance of the internal part of the culture system at different wavelength lights at a certain time period through the color and brightness information inside the photo. The utility model adopts a camera combined with a reference plate and an ultraviolet-visible light source to collect the optical information in the microorganism culture system in a non-invasive manner, which not only reduces the workload of the operator, but also avoids experimental errors caused by human factors; at the same time, both the camera and the reference plate are arranged outside the culture system and do not intervene in the internal part of the culture system, so there is no risk of contamination to its internal part. Description of the Drawings
[0021] Figure 1 This is the overall schematic diagram of the optical information automatic acquisition device described in the present utility model.
[0022] Figure 2 is Figure 1 The schematic diagram after removing the support frame.
[0023] Figure 3 is Figure 2 The front view of
[0024] Figure 4 is Figure 3 The schematic diagram of the camera and the second ultraviolet-visible light source in
[0025] Figure 5 is Figure 4 The top view of
[0026] Figure 6 is Figure 2 The schematic diagram of the light rays detected by the second ultraviolet-visible light source.
[0027] Figure 7 is Figure 2 The schematic diagram of the light rays detected through the reference plate.
[0028] Figure 8 is Figure 1 The patterns and lines printed on the reference plate in
[0029] In the figure: 1 - the first ultraviolet-visible light source, 2 - the reference plate, 3 - the culture carrier, 4 - the carrier plate, 51 - the camera, 52 - the second ultraviolet-visible light source, 6 - the column, 7 - the reinforcing plate. Specific embodiments
[0030] The present utility model will be further described in detail below in conjunction with the accompanying drawings and specific embodiments, which is an explanation rather than a limitation of the present utility model.
[0031] An optical information automatic acquisition device for microorganism culture according to the present utility model includes a first ultraviolet-visible light source 1, a reference plate 2, a carrier plate 4, a camera 51, a second ultraviolet-visible light source 52, and a support frame. The culture carrier 3 needs to meet three conditions: being transparent, having an opening, and having a flat bottom, which is convenient for light source illumination, camera 51 photography, light entry, and placement on the carrier plate 4. Therefore, conical flasks, beakers, flat-bottomed flasks, and culture dishes can be selected. In the accompanying drawings of the present utility model, conical flasks that are relatively common in experiments are selected as the culture carrier 3 for illustration.
[0032] As Figure 1 shown, a reference plate 2 is fixed above the carrier plate 4 through a support frame. As Figure 8As shown, on the lower surface of the reference plate 2, patterns and lines for extracting RGB color information, resolution, grayscale, and sharpness are printed according to international standards. By collecting the changes in RGB values of the positions of the eight-color discs composed of the upper left and lower right corners during the cultivation process, the change in RGB color information is collected. By collecting the image information of the grayscale area (six rectangular blocks of different depths of black set at intervals in the middle on the left side of the figure, and six rectangular blocks of different depths of black set at intervals in the middle on the right side of the figure), the change in grayscale during the cultivation process is determined. By collecting the line information within the circular area at the center position (composed of black blocks distributed at intervals and gradually widening outward from the center along the radius direction), the change in resolution and sharpness within this area is determined. The carrier plate 4 is made of a transparent material, and a culture carrier 3 is placed on its upper surface. The culture carrier 3 carries a prepared culture system. The optical information specifically includes RGB color information, resolution, grayscale, and sharpness. There is a gap between the lower surface of the reference plate 2 and the opening of the culture carrier 3. The opening of the culture carrier 3 is directly below the center of the lower surface of the reference plate 2, and the vertical distance between the opening of the culture carrier 3 and the lower surface of the reference plate 2 is 2 - 4 cm. As Figure 3 and Figure 2 shown, the first ultraviolet-visible light source 1 is placed on the upper surface of the reference plate 2, and they have the same shape. The edge of the first ultraviolet-visible light source 1 is close to the corresponding edge of the reference plate 2. It is necessary to make the light-emitting surface of the first ultraviolet-visible light source 1 located on its lower surface, so that the first ultraviolet-visible light source can illuminate the reference plate 2. The gap reserved between the reference plate 2 and the culture carrier 3 facilitates the entry of ultraviolet or visible light into the culture carrier 3.
[0033] The edge of the carrier plate 4 is flush with the edge of the lower surface of the culture carrier 3. The center of the lower surface of the carrier plate 4 is placed on the lens of the camera 51. A number of second ultraviolet-visible light sources 52 are provided around the lens of the camera 51. All the second ultraviolet-visible light sources 52 are directly below the corresponding positions on the lower surface of the culture carrier 3, facilitating the acquisition of the RGB color information of the culture system in the culture carrier 3. The camera 51 is in a fixed state during use.
[0034] The support frame is composed of two vertically distributed columns 6 and four horizontally distributed and identically long reinforcing plates 7. The two columns 6 are distributed on both sides of the culture carrier 3. The reference plate 2 and the bearing plate 4 are both square and have the same side length. In this way, when the center of the reference plate 2 is directly above the center of the bearing plate 4, their edges are flush. The lower end of each column 6 is fixed at a corner of the bearing plate 4, and the upper end is fixed at the corresponding corner of the reference plate 2. At the same time, the lower ends of the two columns 6 are distributed along the diagonal direction of the bearing plate 4, which facilitates directly and stably placing the culture carrier 3 with the prepared culture system on the bearing plate 4. The reinforcing plates 7 are designed to enhance the stability of the support frame. Their length is the side length of the reference plate 2. It is required that the columns 6 are cylindrical and the diameters of the two columns 6 are the same. The four reinforcing plates 7 form a square protective frame. The upper end of the protective frame is perpendicular to the lower surface of the reference plate 2, and the edges are flush with the corresponding edges of the lower surface of the reference plate 2. In addition, a first notch with an inner wall fitting the outer wall of one column 6 needs to be opened at the junction of two adjacent reinforcing plates 7, and a second notch with an inner wall fitting the outer wall of the other column 6 needs to be opened at the junction of the other two adjacent reinforcing plates 7. In this way, one column 6 can be in contact with the first notch and the other column 6 can be in contact with the second notch, which can further strengthen the fixing effect between the upper end of the column 6 and the reference plate 2.
[0035] As Figure 4 and Figure 5 shown, the camera 51 is specifically composed of a fixing base with a square cross-section and a lens arranged at the center of the fixing base. The lens is a ring structure, and its edge is flush with the edge of the fixing base. The second ultraviolet-visible light source 52 is specifically four, which are respectively arranged at the four corners of the fixing base. At the same time, a certain gap needs to be left between each second ultraviolet-visible light source 52 and the outer edge of the lens. The lens is located at the center of the lower surface of the culture carrier 3, which can ensure that the four second ultraviolet-visible light sources 52 uniformly irradiate the culture system in the culture carrier 3.
[0036] Embodiment
[0037] For an optical information automatic acquisition device for microorganism culture of the present utility model, taking the optical information collection of Escherichia coli in water as an example, two different test functions of the first ultraviolet-visible light source 1 and the second ultraviolet-visible light source 52 in the use of this device are described. The specific operation process of obtaining optical information is as follows:
[0038] Take 200 ml of LB medium, add 10 μl of Escherichia coli for culture, set the culture temperature to 30 °C, and the culture time to 24 hours.
[0039] Step 1, place the culture carrier 3 carrying the prepared culture system on the bearing plate 4, and set the culture temperature to 30 °C.
[0040] Step 2: Turn on the first ultraviolet-visible light source 1, which can emit light at 400 nm, 520 nm, 650 nm, or white light according to experimental requirements. The first ultraviolet-visible light source 1 illuminates the reference plate 2. As shown in Figure 7 , the camera 51 receives the light from the reference plate 2 through the transparent carrier plate 4 and the culture carrier 3, and takes a picture of the reference plate 2 to obtain the optical reference value of the culture system, including RGB color information, resolution, grayscale, and sharpness. Then, turn off the first ultraviolet-visible light source 1 and the camera 51. By performing spectral-RGB correspondence analysis on the RGB color information alone, the spectral information in the culture system can be further analyzed, thereby preliminarily analyzing the relevant contents in the culture system.
[0041] Step 3: Turn on the second ultraviolet-visible light source 52, which can emit light at 400 nm, 520 nm, 650 nm, or white light according to experimental requirements. The second ultraviolet-visible light source 52 illuminates the culture system in the culture carrier 3. As shown in Figure 6 , the camera 51 takes a picture of the culture system through the transparent carrier plate 4 and the culture carrier 3 to obtain the RGB color information of the culture system. Then, turn off the second ultraviolet-visible light source 52 and the camera 51.
[0042] Repeat the processes of Step 2 and Step 3 every 4 hours until the 24-hour culture ends, and the optical information curve of the culture system can be obtained. The optical information therein includes RGB color information, grayscale, sharpness, and resolution.
[0043] Taking the RGB color information in Step 3 as an example, the six measured RGB values are (91, 86, 67), (94, 88, 73), (97, 95, 79), (102, 101, 83), (106, 105, 96), and (110, 113, 109). Further convert them into absorbance values at 650 nm, specifically 0.2 OD, 0.4 OD, 0.7 OD, 0.9 OD, 1.1 OD, and 1.5 OD, to obtain the corresponding absorbance curve.
[0044] In addition, parallel experiments are carried out, and the data finally obtained by the ultraviolet spectrophotometer are 0.25 OD, 0.44 OD, 0.73 OD, 0.96 OD, 1.17 OD, and 1.56 OD. It can be seen that these values are in good agreement with the above data.
[0045] The turbidity of the culture system can be measured through the grayscale and sharpness in Step 2. For example, if the test results for grayscale within 24 hours are 23%, 28%, 32%, 38%, 43%, 47% respectively, and the test results for sharpness are 33, 42, 50, 58, 68, 76 respectively, then the corresponding turbidities after calculation are 17 degrees, 48 degrees, 76 degrees, 109 degrees, 140 degrees, 181 degrees. The content of the culture system can be measured through the resolution in Step 2 and the uniformity in Step 3. Among them, by combining the resolution information in Step 2 with the uniformity collected subsequently, the photos directly obtained through Step 3 are analyzed to judge the reaction degree of the system, that is, whether flocculent or other forms of solids are produced in the reaction system.
Claims
1. An automatic optical information acquisition device for microbial culture, characterized in that, It includes a first ultraviolet and visible light source (1), a carrier plate (4) and a camera (51). Above the carrier plate (4), a reference plate (2) is fixed. The carrier plate (4) is made of a transparent material. On the upper surface of the carrier plate (4), a culture carrier (3) is placed. The culture carrier (3) carries a culture system from which optical information is to be obtained. The optical information includes RGB color information, resolution, grayscale and sharpness. There is a gap between the lower surface of the reference plate (2) and the opening of the culture carrier (3). On the lower surface of the reference plate (2), patterns and lines for extracting RGB color information, resolution, grayscale and sharpness are printed respectively. The first ultraviolet and visible light source (1) is arranged on the upper surface of the reference plate (2). The light-emitting surface of the first ultraviolet and visible light source (1) is located on its lower surface. The camera (51) is arranged on the lower surface of the carrier plate (4). Around the lens of the camera (51), a number of second ultraviolet and visible light sources (52) are arranged. All the second ultraviolet and visible light sources (52) are directly below the corresponding positions on the lower surface of the culture carrier (3).
2. The optical information automatic acquisition device for microorganism culture according to claim 1, characterized in that The culture carrier (3) is a conical flask, a beaker, a flat-bottomed flask or a petri dish.
3. The optical information automatic acquisition device for microorganism culture according to claim 1, wherein The first ultraviolet and visible light source (1) is placed on the upper surface of the reference plate (2), and the edge of the first ultraviolet and visible light source (1) is close to the corresponding edge of the reference plate (2).
4. The optical information automatic acquisition device for microorganism culture according to claim 3, characterized in that The opening of the culture carrier (3) is directly below the center of the lower surface of the reference plate (2).
5. The optical information automatic acquisition device for microorganism culture according to claim 4, characterized in that The vertical distance between the opening of the culture carrier (3) and the lower surface of the reference plate (2) is 2 - 4 cm.
6. The optical information automatic acquisition device for microorganism culture according to claim 1, characterized in that, The edge of the carrier plate (4) is flush with the edge of the lower surface of the culture carrier (3), and the center of the lower surface of the carrier plate (4) is placed on the lens of the camera (51).
7. The optical information automatic acquisition device for microorganism culture according to claim 1, characterized in that The camera (51) includes a fixing base with a square cross-section and a lens arranged at the center of the fixing base. The lens is a ring structure, and the edge of the lens is flush with the edge of the fixing base. There are four second ultraviolet and visible light sources (52), which are respectively distributed at the four corners of the fixing base. There is a gap between each second ultraviolet and visible light source (52) and the outer edge of the lens.
8. The optical information automatic acquisition device for microorganism culture according to claim 7, characterized in that, The lens is located at the center of the lower surface of the culture carrier (3).
9. The optical information automatic acquisition device for microorganism culture according to claim 1, wherein It also includes two vertically distributed columns (6) distributed on both sides of the culture carrier (3). Both the reference plate (2) and the carrier plate (4) are square and have the same side length. The center of the reference plate (2) is directly above the center of the carrier plate (4). The lower end of each column (6) is fixed at a corner of the carrier plate (4), and the upper end of each column (6) is fixed at the corresponding corner of the reference plate (2). The lower ends of the two columns (6) are distributed along the diagonal direction of the carrier plate (4).
10. The optical information automatic acquisition device for microorganism culture according to claim 9, characterized in that, It further includes four reinforcing plates (7) that are horizontally distributed and have the same length. The length of the reinforcing plate (7) is the side length of the reference plate (2). The four reinforcing plates (7) form a square protective frame. The upper end of the protective frame is perpendicular to the lower surface of the reference plate (2). The edge of each reinforcing plate (7) is flush with the edge of the corresponding side of the lower surface of the reference plate (2). The cross-section of the column (6) is circular, and the diameters of the two columns (6) are the same. A first notch with an inner wall that fits the outer wall of one column (6) is provided at the joint of two adjacent reinforcing plates (7). Additionally, a second notch with an inner wall that fits the outer wall of the other column (6) is provided at the joint of two adjacent reinforcing plates (7). One column (6) is in contact with the first notch, and the other column (6) is in contact with the second notch.