Microfluidic biochemical analyzer

Abstract

The utility model relates to the field of microfluidics, and discloses a microfluidic biochemical analyzer which comprises a box body, a box door is rotationally connected to the center of the upper end face of the box body, handles are arranged at the upper positions of the centers of the two side walls of the box body, an analysis box is arranged at the position, close to one side, of the center in the box body, and a hot box is arranged at the front position of the center of one side in the box body. A cold box is arranged on the inner wall of a box body on the rear end face of the hot box, a protection fixing structure is arranged at the center in the analysis box, and an analyzer body is arranged at the center in the protection fixing structure. According to the utility model, through the combination of the compressor, the condenser, the evaporator, the circulating pump, the circulating pipe and the four fans, the temperature control of the whole system can be realized, the air convection is enhanced, the temperature uniformity is improved, and the influence of the temperature gradient on the experimental result is avoided, and meanwhile, the temperature control structure has better controllability and stability, and is convenient to operate. The requirements under different experiment conditions can be met.

Description

Technical Field

[0001] The utility model relates to the field of microfluidics, in particular to a microfluidic biochemical analyzer. Background Art

[0002] A microfluidic biochemical analyzer is an instrument that uses microfluidic technology to process and analyze biological samples. Microfluidic technology is a research field based on microchannels and microscale-related experimental techniques. It flows and mixes samples and reagents in micro-liter volumes through microchannels, achieving efficient processing and precise analysis of biological samples.

[0003] Since traditional microfluidic biochemical analyzers may not be able to effectively control the temperature of the system, resulting in large fluctuations in the temperature gradient, affecting the accuracy and repeatability of experimental results, and there may be inaccurate sample delivery due to unreasonable design or improper operation, thus affecting the accuracy of analysis results. Therefore, those skilled in the art have provided a microfluidic biochemical analyzer to solve the problems raised in the above background art. Content of the Utility Model

[0004] The purpose of the utility model is to solve the deficiencies existing in the prior art, and a microfluidic biochemical analyzer is proposed. Through the combination of a compressor, a condenser, an evaporator, a circulation pump, a circulation pipe, and four fans, the temperature control of the entire system can be achieved, enhancing air convection, improving temperature uniformity, and avoiding the influence of temperature gradient on experimental results. At the same time, this temperature control structure has better controllability and stability, and can meet the requirements under different experimental conditions. By adopting the design of a sample placement rack and placement grooves, the mutual interference and displacement between samples are avoided, improving the efficiency and accuracy of signal transmission. And through the structural design of a peristaltic pump, an output pipe, and an extraction pipe, the extraction of the sample to be analyzed is realized, so that the sample can be accurately transported to the microfluidic chip inside the analysis instrument for analysis. Through the structural design of a buffer layer, an elastic layer, a spring, and a clamping plate, external vibration and impact can be effectively absorbed, protecting the microscopic structure inside the analysis instrument, and improving the stability and reliability of the instrument.

[0005] To achieve the above object, the utility model provides the following technical solution: A microfluidic biochemical analyzer, including a box body, a box door is rotatably connected to the center of the upper end face of the box body, handles are provided at the upper center of both side walls of the box body, an analysis box is provided at the center of the box body close to one side inside, a hot box is provided at the center of the front of one side inside the box body, a cold box is provided on the inner wall of the box body at the rear end face of the hot box, a protection and fixation structure is provided at the center of the analysis box, an analyzer body is provided at the center of the protection and fixation structure, a temperature control structure is provided inside the cold box and the hot box, a sample placement structure is provided at the front center of the lower inner wall of the analysis box, and an extraction structure is provided at the rear center of the lower inner wall of the analysis box;

[0006] Through the above technical solution, through the combination of a compressor, a condenser, an evaporator, a circulation pump, a circulation pipe, and four fans, the temperature control of the entire system can be achieved, enhancing air convection, improving temperature uniformity, avoiding the influence of temperature gradient on experimental results. At the same time, this temperature control structure has better controllability and stability, and can meet the requirements under different experimental conditions. By adopting the design of the sample placement rack and placement slots, the mutual interference and displacement between samples are avoided, the efficiency and accuracy of signal transmission are improved, and through the structural design of a peristaltic pump, an output pipe, and an extraction pipe, the extraction of the sample to be analyzed is realized, so that the sample can be accurately transported to the microfluidic chip inside the analytical instrument for analysis. Through the structural design of a buffer layer, an elastic layer, springs, and a clamping plate, external vibration and impact can be effectively absorbed, protecting the microscopic structure inside the analytical instrument and improving the stability and reliability of the instrument.

[0007] Further, the protection and fixation structure includes a buffer layer, an elastic layer, four springs, and two clamping plates. The buffer layer is arranged at the center of the lower inner wall of the analysis box. The elastic layer is sleeved on the inner side wall of the buffer layer. The four springs are respectively arranged at the two sides near the center of the front inner wall and the two sides near the center of the rear inner wall of the elastic layer. The two clamping plates are respectively arranged at the front and rear of the center of the lower inner wall of the elastic layer on the end parts of the four springs. The analyzer body is arranged at the center of the lower inner wall of the elastic layer;

[0008] Through the above technical solution, when external vibration or impact occurs, this energy will first be absorbed by the buffer layer, reducing the impact force on the analyzer body. At the same time, the existence of the elastic layer can slow down the transmission of impact energy, protecting the microscopic structure inside the instrument. The setting of the four springs increases the stability and balance of the structure, can balance and hold the analyzer body, reducing the interference of vibration on the analyzer body. The function of the clamping plate is to fix the analyzer body in place, ensuring the stability of the whole structure.

[0009] Further, the sample placement structure includes a sample placement rack and a plurality of placement slots. The sample placement rack is arranged at the front of the center of the lower inner wall of the analysis box. The plurality of placement slots are arranged in a rectangular array on the upper end surface of the sample placement rack;

[0010] Through the above technical solution, during use, the user can place the sample to be analyzed in the placement slots. Through reasonable design and arrangement, the samples can be evenly supported in the placement slots, avoiding the mutual interference and displacement between samples. In addition, the position of the sample placement structure is relatively forward, which can make the sample closer to the analyzer body, reducing the distance between the sample and the instrument, and improving the efficiency and accuracy of signal transmission.

[0011] Further, the extraction structure includes a peristaltic pump, an output pipe, and an extraction pipe. The peristaltic pump is arranged at the center and near the rear of the lower inner wall of the analysis box. The extraction pipe is arranged at the output end of the peristaltic pump. The output pipe is arranged at the input end of the peristaltic pump. The input end of the output pipe sequentially penetrates through the rear inner wall of the analysis box and the rear inner wall of the box body and leads to the outside of the box body. The output end of the extraction pipe is arranged at one side near the upper end of the analyzer body;

[0012] Through the above technical solution, in the experimental operation, the user can select the sample to be extracted, place the extraction pipe above the sample, open the air valve of the output pipe of the peristaltic pump, start the peristaltic pump, and the peristaltic pump pumps the sample into the output pipe. The sample passes through the input end of the output pipe, passes through the analysis box and the box body, and then leads to the outside of the box body. Finally, the sample is extracted onto the microfluidic chip inside the analyzer body.

[0013] Further, the temperature control structure includes a compressor, a condenser, an evaporator, a circulation pump, a circulation pipe, and four fans. The compressor is arranged at the center of the rear inner wall of the hot box. The condenser is arranged at the output end of the compressor. The output end of the condenser sequentially penetrates through the front inner wall of the hot box and the rear end face of the cold box and leads to the inside of the cold box. The evaporator is arranged at the output end of the condenser. The circulation pump is arranged at the output end of the evaporator. The circulation pipe is arranged at the output end of the circulation pump. The output end of the circulation pipe sequentially penetrates through the rear inner wall of the cold box and the front end face of the hot box and leads to the inside of the hot box, and the end is fixedly connected to the input end of the compressor. The four fans are respectively arranged at the center and near the front and the center and near the rear of one inner side wall of the cold box and the hot box;

[0014] Through the above technical solution, when the system is running, the compressor sends high-pressure gas into the condenser, cools the gas into a liquid state through the cooling effect of the condenser. The output end of the condenser sequentially passes through the front inner wall of the hot box and the rear end face of the cold box and leads to the inside of the cold box. The liquid output by the condenser is cooled by the evaporator, and the cooled liquid is sent back into the compressor by the circulation pump. The hot air released by the condenser and the cold air released by the evaporator are sucked by the fans to control the temperature inside the analysis box, enhance air convection, improve temperature uniformity, and avoid the influence of temperature gradient on the experimental results.

[0015] Further, a microfluidic chip is arranged at the center and near one side inside the analyzer body;

[0016] Through the above technical solution, the microfluidic chip contains microchannels and microreactors. The reagent and the sample to be tested are mixed through the microchannels, chemical reactions are carried out in the microreactors, and then the reaction products are sent into the detection module through the microchannels for analysis.

[0017] Further, two discharge solenoid valves are arranged horizontally at the center and near one side of the front end face of the box body and at the center and near one side of the rear end face of the box body;

[0018] Through the above technical solution, by utilizing the switching operations of these two discharge solenoid valves, effective discharge control can be carried out for the temperatures inside the cold box and the hot box respectively, effectively avoiding the situation where the temperature of the cold box is too low or the temperature of the hot box is too high, thereby ensuring that the temperature inside the box always remains within an appropriate range.

[0019] The utility model has the following beneficial effects:

[0020] 1. In the utility model, through the combination of a compressor, a condenser, an evaporator, a circulation pump, a circulation pipe and four fans in the microfluidic biochemical analyzer, the temperature control of the entire system can be achieved, enhancing air convection, improving temperature uniformity, avoiding the influence of temperature gradient on experimental results, and at the same time, this temperature control structure has better controllability and stability, and can meet the requirements under different experimental conditions.

[0021] 2. In the utility model, through the design of the sample placement rack and the placement groove, the mutual interference and displacement between samples are avoided, the efficiency and accuracy of signal transmission are improved, and through the structural design of the peristaltic pump, the output pipe and the extraction pipe, the extraction of the sample to be analyzed is realized, so that the sample can be accurately transported to the microfluidic chip inside the analysis instrument for analysis.

[0022] 3. In the utility model, through the structural design of the buffer layer, the elastic layer, the spring and the clamping plate, the external vibration and impact are effectively absorbed, the microscopic structure inside the analysis instrument is protected, and the stability and reliability of the instrument are improved. Description of the Drawings

[0023] Figure 1 is a perspective view of a microfluidic biochemical analyzer proposed by the utility model;

[0024] Figure 2 is a three-dimensional side sectional view of a microfluidic biochemical analyzer proposed by the utility model;

[0025] Figure 3 is a three-dimensional front sectional view of a microfluidic biochemical analyzer proposed by the utility model;

[0026] Figure 4 is a side sectional view of a microfluidic biochemical analyzer proposed by the utility model.

[0027] Legend Explanation:

[0028] 1. Box body; 2. Box door; 3. Handle; 4. Analysis box; 5. Protective and fixing structure; 501. Buffer layer; 502. Elastic layer; 503. Spring; 504. Clamping plate; 6. Sample placement structure; 601. Sample placement rack; 602. Placement groove; 7. Extraction structure; 701. Peristaltic pump; 702. Output pipe; 703. Extraction pipe; 8. Heating box; 9. Temperature control structure; 901. Compressor; 902. Condenser; 903. Evaporator; 904. Circulation pump; 905. Circulation pipe; 906. Fan; 10. Cooling box; 11. Discharge solenoid valve; 12. Analyzer body; 13. Microfluidic chip. Detailed implementation manners

[0029] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0030] Referring to Figures 1-4 , an embodiment provided by the present invention: A microfluidic biochemical analyzer includes a box body 1. A box door 2 is rotatably connected to the center of the upper end surface of the box body 1. Handles 3 are provided at the upper center of both side walls of the box body 1. An analysis box 4 is provided at the center and near one side inside the box body 1. A heating box 8 is provided at the center and near the front of one side inside the box body 1. A cooling box 10 is provided on the inner wall of the box body at the rear end surface of the heating box 8. A protective and fixing structure 5 is provided at the center inside the analysis box 4. An analyzer body 12 is provided at the center inside the protective and fixing structure 5. A temperature control structure 9 is provided inside the cooling box 10 and the heating box 8. A sample placement structure 6 is provided at the center and near the front of the lower inner wall of the analysis box 4. An extraction structure 7 is provided at the center and near the rear of the lower inner wall of the analysis box 4.

[0031] Place the sample to be tested in the sample placement structure 6, and then extract the sample into the microfluidic chip 13 through the extraction structure 7 for analysis. During the analysis process, different reagents or solutions will be input into the sample in the microfluidic chip 13, and then the flow rate and position of the sample will be controlled by microfluidic technology to finally obtain the required analysis results. At the same time, the temperature control structure 9 in the analyzer can precisely control the temperature of the sample to ensure the accuracy of the experiment. The microfluidic chip 13 is provided at one side near the center inside the analyzer body 12. The microfluidic chip 13 contains tiny channels and microreactors. The reagent is mixed with the sample to be tested through the tiny channels, and chemical reactions occur in the microreactors. Then, the reaction products are sent to the detection module through the microchannels for analysis. Two discharge solenoid valves 11 are arranged horizontally at one side near the center of the front end face and at one side near the center of the rear end face of the box body 1. By using the switching operations of these two discharge solenoid valves 11, effective discharge control can be carried out for the temperatures inside the cold box 10 and the hot box 8 respectively, and the situation where the temperature of the cold box 10 is too low or the temperature of the hot box 8 is too high can be effectively avoided, so as to ensure that the temperature inside the box body 1 always remains within an appropriate range.

[0032] The protection and fixation structure 5 includes a buffer layer 501, an elastic layer 502, four springs 503 and two clamping plates 504. The buffer layer 501 is arranged at the center of the lower inner wall of the analysis box 4. The elastic layer 502 is sleeved on the inner side wall of the buffer layer 501. Four springs 503 are respectively arranged at two sides near the center of the front inner wall and at two sides near the center of the rear inner wall of the elastic layer 502. Two clamping plates 504 are respectively arranged at the front center and the rear center of the lower inner wall of the elastic layer 502 on the end parts of the four springs 503. The analyzer body 12 is arranged at the center of the lower inner wall of the elastic layer 502. When external vibration or impact occurs, this energy will first be absorbed by the buffer layer 501, reducing the impact force on the analyzer body 12. At the same time, the existence of the elastic layer 502 can slow down the transmission of the impact energy and protect the microscopic structure inside the instrument. The setting of the four springs 503 increases the stability and balance of the structure, can balance and hold the analyzer body 12, and reduces the interference of vibration on the analyzer body 12. The function of the clamping plate 504 is to fix the analyzer body 12 in place to ensure the stability of the whole structure.

[0033] The sample placement structure 6 includes a sample placement rack 601 and a plurality of placement grooves 602. The sample placement rack 601 is arranged at the center and front of the lower inner wall of the analysis box 4. The plurality of placement grooves 602 are arranged in a rectangular pattern on the upper end surface of the sample placement rack 601. During use, the user can place the sample to be analyzed in the placement groove 602. Through reasonable design and arrangement, the sample can be evenly supported in the placement groove 602, avoiding mutual interference and displacement between samples. In addition, the forward position of the sample placement structure 6 can make the sample closer to the analyzer body 12, reducing the distance between the sample and the instrument and improving the efficiency and accuracy of signal transmission.

[0034] The extraction structure 7 includes a peristaltic pump 701, an output pipe 702, and an extraction pipe 703. The peristaltic pump 701 is arranged at the center and rear of the lower inner wall of the analysis box 4. The extraction pipe 703 is arranged at the output end of the peristaltic pump 701, and the output pipe 702 is arranged at the input end of the peristaltic pump 701. The input end of the output pipe 702 sequentially penetrates the rear inner wall of the analysis box 4 and the rear inner wall of the box body 1 and leads to the outside of the box body 1. The output end of the extraction pipe 703 is arranged at one side near the upper end of the analyzer body 12. During the experimental operation, the user can select the sample to be extracted, place the extraction pipe 703 above the sample, open the air valve of the output pipe 702 of the peristaltic pump 701, and start the peristaltic pump 701. The peristaltic pump 701 pumps the sample into the output pipe 702, and the sample passes through the analysis box 4 and the box body 1 through the input end of the output pipe 702 and then leads to the outside of the box body 1. Finally, the sample is extracted onto the microfluidic chip 13 inside the analyzer body 12.

[0035] The temperature control structure 9 includes a compressor 901, a condenser 902, an evaporator 903, a circulation pump 904, a circulation pipe 905, and four fans 906. The compressor 901 is arranged at the center of the rear inner wall of the hot box 8. The condenser 902 is arranged on the output end of the compressor 901. The output end of the condenser 902 sequentially passes through the front inner wall of the hot box 8 and the rear end face of the cold box 10 and leads to the inside of the cold box 10. The evaporator 903 is arranged on the output end of the condenser 902. The circulation pump 904 is arranged on the output end of the evaporator 903. The circulation pipe 905 is arranged on the output end of the circulation pump 904. The output end of the circulation pipe 905 sequentially passes through the rear inner wall of the cold box 10 and the front end face of the hot box 8 and leads to the inside of the hot box 8, and the end is fixedly connected to the input end of the compressor 901. The four fans 906 are respectively arranged at the positions slightly forward and slightly backward of the center of one inner side wall of the cold box 10 and the hot box 8. When the system is running, the compressor 901 sends high-pressure gas into the condenser 902, and the gas is cooled into a liquid state through the cooling effect of the condenser 902. The output end of the condenser 902 sequentially passes through the front inner wall of the hot box 8 and the rear end face of the cold box 10 and leads to the inside of the cold box 10. The liquid output by the condenser 902 is cooled by the evaporator 903, and the cooling liquid is sent back into the compressor 901 through the circulation pump 904. The hot air released by the condenser 902 and the cold air released by the evaporator 903 are sucked by the fans 906 to control the temperature inside the analysis box 4, enhance air convection, improve temperature uniformity, and avoid the influence of temperature gradient on the experimental results.

[0036] Working principle: During use, the user can place the sample to be analyzed in the placement groove 602. Through reasonable design and arrangement, the samples can be evenly supported in the placement groove 602, avoiding mutual interference and displacement between the samples. In addition, the position of the sample placement structure 6 is relatively forward, which can make the samples closer to the analyzer body 12, reduce the distance between the samples and the instrument, and improve the efficiency and accuracy of signal transmission. During the experiment operation, the user can select the sample to be extracted, place the extraction tube 703 above the sample, open the air valve of the output pipe 702 of the peristaltic pump 701, and start the peristaltic pump 701. The peristaltic pump 701 pumps the sample into the output pipe 702, passes through the analysis box 4 and the box body 1 through the input end of the output pipe 702 and leads to the outside of the box body 1. Finally, the sample is extracted onto the microfluidic chip 13 inside the analyzer body 12. During the analysis process, different reagents or solutions will be input into the samples in the microfluidic chip 13, and then the flow rate and position of the samples are controlled through microfluidic technology to finally obtain the required analysis results. At the same time, the temperature control structure 9 in this analyzer can accurately control the temperature of the samples to ensure the accuracy of the experiment.

[0037] When vibrations or impacts occur from the outside, this energy is first absorbed by the buffer layer 501, reducing the impact force on the analyzer body 12. At the same time, the presence of the elastic layer 502 can slow down the transmission of the impact energy and protect the microscopic structure inside the instrument. The setting of the four springs 503 increases the stability and balance of the structure, can balance and hold the analyzer body 12, reducing the interference of vibrations on the analyzer body 12. The function of the clamping plate 504 is to fix the analyzer body 12 in place, ensuring the stability of the entire structure. When the system is running, the compressor 901 sends high-pressure gas into the condenser 902, and the gas is cooled into a liquid state through the cooling effect of the condenser 902. The output end of the condenser 902 passes through the front inner wall of the hot box 8 and the rear end face of the cold box 10 in sequence and leads to the inside of the cold box 10. The liquid output by the condenser 902 is cooled by the evaporator 903, and the cooling liquid is sent back into the compressor 901 through the circulation pump 904. The hot air released by the condenser 902 and the cold air released by the evaporator 903 are sucked by the fan 906 to control the temperature inside the analysis box 4, enhancing air convection, improving temperature uniformity, and avoiding the influence of temperature gradients on the experimental results.

[0038] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A microfluidic biochemical analyzer, comprising a box body (1), characterized in that: A box door (2) is rotatably connected to the center of the upper end surface of the box body (1). Handles (3) are provided at the upper centers of both side walls of the box body (1). An analysis box (4) is provided at one side center near the interior center of the box body (1). A heat box (8) is provided at the front center of one side inside the box body (1). A cold box (10) is provided on the inner wall of the rear end surface of the box body (1) of the heat box (8). A protection and fixation structure (5) is provided at the center of the analysis box (4). An analyzer body (12) is provided at the center of the protection and fixation structure (5). A temperature control structure (9) is provided inside the cold box (10) and the heat box (8). A sample placement structure (6) is provided at the front center of the lower inner wall of the analysis box (4). An extraction structure (7) is provided at the rear center of the lower inner wall of the analysis box (4).

2. The microfluidic biochemical analyzer according to claim 1, characterized in that: The protection and fixation structure (5) includes a buffer layer (501), an elastic layer (502), four springs (503), and two clamping plates (504). The buffer layer (501) is provided at the center of the lower inner wall of the analysis box (4). The elastic layer (502) is sleeved on the inner side wall of the buffer layer (501). The four springs (503) are respectively provided at the front center near both sides and the rear center near both sides of the inner wall of the elastic layer (502). The two clamping plates (504) are respectively provided at the front center and the rear center of the lower inner wall of the elastic layer (502) on the ends of the four springs (503). The analyzer body (12) is provided at the center of the lower inner wall of the elastic layer (502).

3. The microfluidic biochemical analyzer according to claim 1, characterized in that: The sample placement structure (6) includes a sample placement rack (601) and a plurality of placement grooves (602). The sample placement rack (601) is provided at the front center of the lower inner wall of the analysis box (4). The plurality of placement grooves (602) are arranged in a rectangular pattern on the upper end surface of the sample placement rack (601).

4. A microfluidic biochemical analyzer according to claim 1, characterized in that: The extraction structure (7) includes a peristaltic pump (701), an output pipe (702), and an extraction pipe (703). The peristaltic pump (701) is provided at the rear center of the lower inner wall of the analysis box (4). The extraction pipe (703) is provided at the output end of the peristaltic pump (701). The output pipe (702) is provided at the input end of the peristaltic pump (701). The input end of the output pipe (702) sequentially penetrates the rear inner wall of the analysis box (4) and the rear inner wall of the box body (1) and leads to the outside of the box body (1). The output end of the extraction pipe (703) is provided at one side near the upper end of the analyzer body (12).

5. The microfluidic biochemical analyzer according to claim 1, wherein: The temperature control structure (9) includes a compressor (901), a condenser (902), an evaporator (903), a circulation pump (904), a circulation pipe (905) and four fans (906). The compressor (901) is arranged at the center of the rear inner wall of the hot box (8). The condenser (902) is arranged at the output end of the compressor (901). The output end of the condenser (902) sequentially penetrates through the front inner wall of the hot box (8) and the rear end face of the cold box (10) and leads to the inside of the cold box (10). The evaporator (903) is arranged at the output end of the condenser (902). The circulation pump (904) is arranged at the output end of the evaporator (903). The circulation pipe (905) is arranged at the output end of the circulation pump (904). The output end of the circulation pipe (905) sequentially penetrates through the rear inner wall of the cold box (10) and the front end face of the hot box (8) and leads to the inside of the hot box (8), and the end is fixedly connected to the input end of the compressor (901). The four fans (906) are respectively arranged at the positions close to the front and the rear of the center on one inner side wall of the cold box (10) and the hot box (8).

6. The microfluidic biochemical analyzer according to claim 1, wherein: A microfluidic chip (13) is arranged at a position close to one side of the center inside the analyzer body (12).

7. The microfluidic biochemical analyzer according to claim 1, wherein: Two discharge solenoid valves (11) are arranged in a horizontal row at positions close to one side of the center on the front end face and the rear end face of the box body (1).

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