Stem cell preparation cryopreservation bag convenient for cleaning and replacing auxiliary materials

Abstract

The utility model discloses a stem cell preparation cryopreservation bag convenient to clean and replace auxiliary materials, which comprises a cryopreservation bag body, cryopreservation bag labels are arranged on both sides of the cryopreservation bag body, and an injection inlet, a pipeline and a sample reserving bag are respectively arranged at the top of the cryopreservation bag body. The bottom of the cryopreservation bag body is provided with a buffer solution inlet, a buffer solution outlet, a buffer solution inlet pipeline, an injection solution outlet and an injection solution outlet pipeline. The stem cell preparation filling and cleaning device can be connected with different filling and cleaning devices, and automatic filling and exhausting, stem cell preparation cleaning and auxiliary material replacement are achieved; filling, exhausting, cryopreservation and cleaning of the stem cell preparation, replacement of auxiliary materials and reinfusion and use of the preparation can be achieved without the help of other equipment.

Description

Technical Field

[0001] The utility model belongs to the technical field of cryopreservation of stem cell preparations, and particularly relates to a cryopreservation bag for stem cell preparations which is convenient for cleaning and replacing auxiliary materials. Background Art

[0002] A stem cell preparation refers to a stem cell product extracted, cultured and processed from different sources (such as bone marrow, umbilical cord blood, adipose tissue, etc.) through biotechnology. Stem cells are a type of cells with self-renewal and multi-directional differentiation potential, and can develop into various types of cells, so they have important applications in regenerative medicine and tissue engineering.

[0003] The cryopreservation of stem cell preparations is a commonly used preservation technique, aiming to maintain the activity and function of stem cells for future use. The existing technology mainly cryopreserves stem cell preparations through cryopreservation bags, but there are some defects and deficiencies to be improved: (1) The exhaust of some existing cryopreservation bags is relatively difficult, the exhaust process is cumbersome and unsafe, and due to the structural design, the effect is not good when used in combination with automated equipment; (2) Some existing cryopreservation bags need to be cleaned and filled with the help of other equipment, which is expensive, and a large number of commercial supporting facilities need to be put into use, not only the input cost is high, but also professional personnel are required to operate. Therefore, in view of the above problems, it is of great significance to provide a cryopreservation bag for stem cell preparations which is convenient for cleaning and replacing auxiliary materials according to the utility model. Summary of the Utility Model

[0004] The utility model provides a cryopreservation bag for stem cell preparations which is convenient for cleaning and replacing auxiliary materials, which can be connected to different filling and cleaning equipment to realize automated filling and exhaust, cleaning of stem cell preparations and replacement of auxiliary materials; it can realize filling and exhaust, cryopreservation, cleaning and replacement of auxiliary materials of stem cell preparations and back-transfusion use of the preparations without the help of other equipment, thus solving the problems in the background art.

[0005] To solve the above technical problems, the utility model is realized through the following technical solutions:

[0006] A cryopreservation bag for stem cell preparations which is convenient for cleaning and replacing auxiliary materials according to the utility model includes a cryopreservation bag body. Both sides of the cryopreservation bag body are provided with cryopreservation bag label places. The top of the cryopreservation bag body is respectively provided with an injection liquid inlet and pipeline and a sample retention bag, and the bottom of the cryopreservation bag body is respectively provided with a buffer liquid inlet and outlet and pipeline and an injection liquid extraction port and pipeline.

[0007] Further, the injection liquid inlet and pipeline include a Luer connector A, an exhaust pipeline, a speed regulating valve, a drip funnel and a trapezoidal interface A.

[0008] Further, the buffer liquid inlet and outlet and pipeline include a Luer connector B and a trapezoidal interface B.

[0009] Furthermore, a cell filter screen is installed inside the trapezoidal interface B.

[0010] Furthermore, the injection liquid extraction port and pipeline include a Luer connector C and a rubber stopper.

[0011] Furthermore, the diameter of the rubber stopper is equal to the inner diameter of the injection liquid extraction port and pipeline.

[0012] The present utility model has the following beneficial effects compared with the prior art:

[0013] (1) When the stem cell preparation freezing bag facilitating cleaning and replacement of adjuvants in the present utility model is in use, it can be connected to different filling and cleaning devices to realize automatic filling and exhaust, cleaning of the stem cell preparation, and replacement of adjuvants.

[0014] (2) When the stem cell preparation freezing bag facilitating cleaning and replacement of adjuvants in the present utility model is in use, it can realize filling and exhaust, freezing, cleaning, replacement of adjuvants of the stem cell preparation, and reinfusion use of the preparation without relying on other devices.

[0015] Of course, it is not necessary for any product implementing the present utility model to simultaneously achieve all the above-mentioned advantages. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings required for describing the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present utility model, and those of ordinary skill in the art can also obtain other drawings based on these drawings without creative efforts.

[0017] Figure 1 It is a schematic structural diagram of a stem cell preparation freezing bag facilitating cleaning and replacement of adjuvants of the present utility model;

[0018] Figure 2 It is a schematic structural diagram of the injection liquid inlet and pipeline in the present utility model;

[0019] Figure 3 It is a schematic structural diagram of the buffer liquid inlet and outlet and pipeline in the present utility model;

[0020] Figure 4 It is a schematic structural diagram of the injection liquid extraction port and pipeline in the present utility model.

[0021] In the drawings, the list of components represented by each reference numeral is as follows:

[0022] 1. Cryopreservation bag body; 2. Label area of cryopreservation bag; 3. Injection inlet and pipeline; 4. Sampling bag; 5. Buffer inlet / outlet and pipeline; 6. Injection extraction port and pipeline; 7. Luer connector A; 8. Exhaust pipeline; 9. Speed regulating valve; 10. Drip funnel; 11. Trapezoidal interface A; 12. Luer connector B; 13. Trapezoidal interface B; 14. Cell filter; 15. Luer connector C; 16. Rubber stopper. Detailed implementation mode

[0023] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the protection scope of the present utility model.

[0024] In the description of the present utility model, it should be understood that the terms "relative", "one end", "inner", "transverse", "end", "both ends", "both sides", "front", "one end face", "the other end face", etc. indicating the orientation or positional relationship are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present utility model.

[0025] Please refer to Figures 1-4 As shown, a stem cell preparation cryopreservation bag for facilitating cleaning and replacing accessories of the present utility model includes a cryopreservation bag body 1. The cryopreservation bag body 1 is a fully sealed structure, mainly used for the sealed storage of stem cell preparations. The cryopreservation bag body 1 adopts a packaging material with low temperature resistance and corrosion resistance, which can achieve long-term storage under liquid nitrogen conditions and high-temperature recovery, thus ensuring tightness and safety. Both sides of the cryopreservation bag body 1 are provided with label areas 2 of the cryopreservation bag. The label areas 2 of the cryopreservation bag are double-mode structures made of the same material as the cryopreservation bag body 1, which can be used for pasting, storing, and sealing labels. The label areas 2 of the cryopreservation bag are located on both sides of the cryopreservation bag body 1, without affecting the use of stem cell preparations and the observation of appearance. The top of the cryopreservation bag body 1 is respectively provided with an injection inlet and pipeline 3 and a sampling bag 4. The sampling bag 4 is hermetically connected to the cryopreservation bag body 1. The sampling bag 4 can be used for sampling stem cell preparations. According to needs, the sampling bag 4 can be single or multiple, ensuring that the samples in the sampling bag 4 are the same as the stem cell preparations in the cryopreservation bag body 1 and are more representative. And just in case, when it is found that there are still a few tiny bubbles in the cryopreservation bag body 1 after the cryopreservation bag pipeline is heat-sealed, the bubbles in the cryopreservation bag body 1 can be discharged into the sampling bag 4, which can be used for both sampling stem cell preparations and collecting bubbles. The bottom of the cryopreservation bag body 1 is respectively provided with a buffer inlet / outlet and pipeline 5 and an injection extraction port and pipeline 6.

[0026] Among them, the injection liquid inlet and pipeline 3 includes a Luer connector A7, an exhaust pipeline 8, a speed regulating valve 9, a drip chamber 10 and a trapezoidal interface A11. The injection liquid inlet and pipeline 3 is hermetically connected to the cryopreservation bag body 1 through the trapezoidal interface A11. The structure of the trapezoidal interface A11 can facilitate the exhaust of the stem cell preparation. The injection liquid inlet and pipeline 3 can be connected to the front end through the Luer connector A7 or directly connected by heat sealing. The exhaust pipeline 8 can be used for exhaust. The exhaust pipeline 8 is connected with a Luer connector + double-layer sterile filter + stop water / gas clamp. The double-layer sterile filter can effectively ensure sterility during exhaust. The stop water / gas clamp is used to connect or prohibit exhaust. The speed regulating valve 9 can adjust the speed during exhaust. The drip chamber 10 can effectively buffer the inflow and outflow of the fluid, which is beneficial to the control of exhaust. And the drip chamber 10 can be used as a temporary storage container or an exhaust balloon. When the front-end exhaust is not thorough, it can be used for further exhaust. Through the injection liquid inlet and pipeline 3, online and offline exhaust can be realized. Even after the filling of the stem cell preparation is completed, manual exhaust can be carried out without any equipment and consumables.

[0027] Among them, the buffer liquid inlet / outlet and pipeline 5 includes a Luer connector B12 and a trapezoidal interface B13. The buffer liquid inlet / outlet and pipeline 5 is hermetically connected to the cryopreservation bag body 1 through the trapezoidal interface B13. The buffer liquid inlet / outlet and pipeline 5 can be connected to the rear end through the Luer connector B12 or directly connected by heat sealing. The buffer liquid inlet / outlet and pipeline 5 can be directly connected to sterile consumables and equipment. By controlling the inflow and outflow of the buffer liquid, the cleaning of the stem cell preparation and the replacement of the medium can be realized. The injection liquid inlet and pipeline 3 and the buffer liquid inlet / outlet and pipeline 5 are both hermetically connected to the cryopreservation bag body 1 and respectively serve as the upstream buffer liquid inlet and the downstream buffer liquid outlet, so that continuous-flow cleaning and medium replacement can be realized.

[0028] Among them, a cell filter net 14 is installed inside the trapezoidal interface B13. Through the cell filter net 14, cells can be effectively intercepted while allowing the buffer liquid to flow in and out.

[0029] Among them, the injection liquid extraction port and pipeline 6 includes a Luer connector C15 and a rubber stopper 16. The injection liquid extraction port and pipeline 6 is hermetically connected to the cryopreservation bag body 1, and can be directly used for the reinfusion of the stem cell preparation or for the extraction by a syringe. The injection liquid extraction port and pipeline 6 is a relatively long rigid pipeline, different from the butterfly buckle of other cryopreservation bags. When a syringe or a reinfusion needle is inserted, it will not exceed the cryopreservation bag body 1. On the one hand, the risk of piercing the cryopreservation bag can be reduced, and on the other hand, the residual dead volume can also be reduced.

[0030] Among them, the diameter of the rubber stopper 16 is equal to the inner diameter of the injection liquid extraction port and pipeline 6. The rubber stopper 16 is made of a sterile sealing rubber material and can be used for repeated punctures and ensure the sealing effect.

[0031] The circuits, electronic components, and chip modules involved in the present utility model are all prior arts, which can be fully realized by those skilled in the art without further elaboration. The content protected by the present utility model does not involve improvements to software and methods either.

[0032] The standard parts used in the application documents can all be purchased from the market. All the components in the application documents can be customized according to the descriptions in the specification and the drawings. The specific connection methods of each part all adopt conventional means such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts, and equipment all adopt conventional models in the prior art. The electrical components described in this article are all electrically connected to the external main controller and the 220V mains power supply, and the main controller can be a conventional known device that controls, such as the LED lamp body, etc.

[0033] The working principle of the present utility model is:

[0034] When the present utility model is in use, the stem cell preparation can be introduced into the cryopreservation bag body 1 through the injection liquid inlet and the pipeline 3, so as to realize the sealed storage of the stem cell preparation through the cryopreservation bag body 1. The injection liquid inlet and the pipeline 3 can be used for online and offline exhaust. Even after the filling of the stem cell preparation is completed, manual exhaust can still be carried out without relying on any equipment and consumables. The buffer liquid inlet and outlet and the pipeline 5 can be directly connected to sterile consumables and equipment. By controlling the inflow and outflow of the buffer liquid, the cleaning of the stem cell preparation and the replacement of the medium are realized. The injection liquid inlet and the pipeline 3 and the buffer liquid inlet and outlet and the pipeline 5 are all hermetically connected to the cryopreservation bag body 1 and are used as the upstream buffer liquid inlet and the downstream buffer liquid outlet respectively, so as to realize continuous flow cleaning and medium replacement. The injection liquid extraction port and the pipeline 6 are hermetically connected to the cryopreservation bag body 1, and can be directly used for the reinfusion of the stem cell preparation or for the extraction by a syringe.

[0035] The preferred embodiments of the present utility model disclosed above are only used to help illustrate the present utility model. The preferred embodiments do not elaborate all the details, nor do they limit the present utility model to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principle and practical application of the present utility model, so that those skilled in the relevant technical field can understand and utilize the present utility model well. The present utility model is only limited by the claims and their full scope and equivalents.

Claims

1. A cryopreservation bag for stem cell preparations that is convenient for cleaning and replacing accessories, characterized in that, It includes a cryopreservation bag body, cryopreservation bag label areas are provided on both sides of the cryopreservation bag body, an injection liquid inlet and pipeline and a sample retention bag are respectively provided at the top of the cryopreservation bag body, and a buffer liquid inlet and outlet and pipeline and an injection liquid extraction port and pipeline are respectively provided at the bottom of the cryopreservation bag body.

2. The cryopreservation bag for stem cell preparation facilitating cleaning and replacement of auxiliary materials according to claim 1, wherein, The injection liquid inlet and pipeline include a Luer connector A, an exhaust pipeline, a speed regulating valve, a drip chamber and a trapezoidal interface A.

3. The cryopreservation bag for stem cell preparations that is convenient for cleaning and replacing auxiliary materials according to claim 1, wherein The buffer liquid inlet and outlet and pipeline include a Luer connector B and a trapezoidal interface B.

4. A cryopreservation bag for stem cell preparations that facilitates cleaning and replacement of adjuvants, characterized in that, A cell filter is installed inside the trapezoidal interface B.

5. A stem cell preparation cryopreservation bag facilitating cleaning and replacement of auxiliary materials according to claim 1, characterized in that, The injection liquid extraction port and pipeline include a Luer connector C and a rubber stopper.

6. The cryopreservation bag for stem cell preparations facilitating cleaning and replacement of accessories according to claim 5, characterized in that The diameter of the rubber stopper is equal to the inner diameter of the injection liquid extraction port and pipeline.

Images