Microinjection needle head fixing device for gene editing
By designing a needle fixing device including a fixing cylinder, a connecting cylinder, a steel wire plunger, an elastic T-shaped plastic cylinder and a conical sleeve, the problem of cumbersome fixing of microinjection needles is solved, and fast and stable needle fixing is achieved.
Patent Information
- Application Number
- CN202422022454.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-20
- Publication Date
- 2025-07-25
- Estimated Expiration
- 2034-08-20
AI Technical Summary
The fixing process of microinjection needles for gene editing is cumbersome, troublesome, and low fixation efficiency.
A needle fixing device including a fixing cylinder, a connecting cylinder, a steel wire plunger, an elastic T-shaped plastic cylinder, a conical sleeve, etc. is designed to achieve rapid fixation of the needle through the movement of the conical sleeve and the elastic force of the spring.
The needle fixing process is simplified, the operating efficiency is improved, and the fixing stability and convenience are ensured.
Smart Images
Figure CN223150537U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of fixing the needle head of a microinjection needle, in particular to a fixing device for the needle head of a microinjection needle for gene editing. Background Technique
[0002] Gene editing, also known as genome editing or genome engineering, is a newly emerging and relatively precise genetic engineering technology or process that can modify specific target genes in the genome of an organism. Early genetic engineering techniques could only randomly insert exogenous or endogenous genetic material into the host genome, while gene editing can precisely edit the desired gene at a specific site. Gene editing relies on genetically engineered nucleases, also known as "molecular scissors", to generate site-specific double-strand breaks (DSBs) at specific positions in the genome, inducing the organism to repair the DSBs through non-homologous end joining (NHEJ) or homologous recombination (HR). Since this repair process is error-prone, it leads to targeted mutations. This targeted mutation is gene editing. Gene microinjection is a method of directly using a microinjector to inject recombinant DNA into the cytoplasm or nucleus of mammalian cells, and it is one of the main methods for introducing genes into mammalian cells in transgenic animal technology.
[0003] When the needle head of a microinjection needle for gene editing is in use, it often needs to be fixed. However, when the common needle head of a microinjection needle for gene editing is fixed, the fixing is generally rather cumbersome, the operation is troublesome, and the fixing efficiency is relatively low. Therefore, new technical solutions need to be designed to solve this problem. Content of the Utility Model
[0004] The purpose of the utility model is to overcome the deficiencies of the prior art, adapt to the actual needs, and provide a fixing device for the needle head of a microinjection needle for gene editing, so as to solve the technical problems that when the needle head of a microinjection needle for gene editing is fixed currently, the fixing is generally rather cumbersome, the operation is troublesome, and the fixing efficiency is relatively low.
[0005] To achieve the purpose of the utility model, the technical solution adopted by the utility model is: design a fixing device for the needle head of a microinjection needle for gene editing, including a fixing cylinder, a connecting cylinder is installed in the middle of one end of the fixing cylinder, a steel wire plunger is installed in the middle of the end of the fixing cylinder close to the connecting cylinder, an installation cylinder is fixedly connected to the outside of the steel wire plunger, an elastic T-shaped plastic cylinder is fixedly connected to one end of the installation cylinder, there are four notches at equal intervals on the outside of the elastic T-shaped plastic cylinder, and a limiting cylinder is slidably sleeved on the outside of the elastic T-shaped plastic cylinder. A spring is sleeved on the outside of the steel wire plunger, and the two ends of the spring are respectively connected to the fixing cylinder and the limiting cylinder. A conical sleeve is slidably sleeved on the outside of the connecting cylinder, a needle head is arranged in the opening in the middle of the conical sleeve, and connecting rods are fixedly connected to both sides of the limiting cylinder. The other ends of the two connecting rods are respectively connected to both sides of the inner cavity of the conical sleeve.
[0006] Preferably, limiting grooves are formed on both sides of the fixed cylinder, limiting blocks are fixedly connected to both sides of the inner cavity of the conical sleeve, and the two limiting blocks are respectively slidably connected in the two limiting grooves.
[0007] Preferably, a plurality of anti-slip rings are installed on the outer side of the conical sleeve, and the plurality of anti-slip rings are equidistantly distributed on the outer side of the conical sleeve.
[0008] Preferably, the spring is always in a compressed state, and the elastic force of the spring is greater than the frictional force between the limiting cylinder and the elastic T-shaped plastic cylinder.
[0009] Preferably, a sealing gasket is fixedly connected to the outer side of the wire plunger, and the sealing gasket has elasticity.
[0010] Compared with the prior art, the beneficial effects of the present utility model are as follows:
[0011] 1. Through the combination of structures such as the conical sleeve, the connecting rod, the limiting cylinder, the spring, the wire plunger, the elastic T-shaped plastic cylinder, the notch and the needle head, the present utility model moves the conical sleeve in the direction of the fixed cylinder, so as to drive the limiting cylinder to approach the fixed cylinder in cooperation with the connecting rod, and at the same time compress the spring, so that the opening of the elastic T-shaped plastic cylinder unfolds. Then, the needle head is inserted into the inner side of the elastic T-shaped plastic cylinder through the opening of the conical sleeve and sleeved on the outer side of the wire plunger. When the needle head moves to contact the installation cylinder and cannot move, after the hand releases the conical sleeve, the elastic force of the spring pushes the conical sleeve to reset, and at the same time drives the limiting cylinder to reset and sleeve on the outer side of the elastic T-shaped plastic cylinder to squeeze and tighten it to limit the needle head. Thus, the staff only needs to push the conical sleeve and insert the needle head to complete the operation, which is convenient for the staff to operate quickly and improves the efficiency of fixing the needle head.
[0012] 2. Through the arrangement of the limiting groove and the limiting block, the present utility model guides and moves the limiting block in the limiting groove, thereby improving the stability of the movement of the conical sleeve outside the fixed cylinder, and avoiding the conical sleeve detaching from the fixed cylinder and affecting the stability of the needle head fixing. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 is a schematic diagram of the overall structure of the present utility model;
[0014] Figure 2 is a sectional view of the overall structure of the present utility model;
[0015] Figure 3 is a schematic diagram of the connection structure between the wire plunger and the elastic T-shaped plastic cylinder of the present utility model;
[0016] Figure 4 is an enlarged view of part A of the present utility model.
[0017] In the figure: 1. Fixed cylinder; 11. Connecting cylinder; 12. Steel wire plunger; 13. Spring; 14. Sealing gasket; 2. Conical sleeve; 21. Anti-slip ring; 3. Needle; 4. Limiting cylinder; 41. Installation cylinder; 42. Elastic T-shaped plastic cylinder; 43. Notch; 44. Connecting rod; 5. Limiting groove; 51. Limiting block. Detailed implementation mode
[0018] The present utility model will be further described below in conjunction with the accompanying drawings and embodiments:
[0019] Embodiment 1: A fixing device for the needle head of a microinjection needle for gene editing, see Figures 1 to 4 , including a fixed cylinder 1, a connecting cylinder 11 is installed in the middle of one end of the fixed cylinder 1, a steel wire plunger 12 is installed in the middle of one end of the fixed cylinder 1 close to the connecting cylinder 11, an installation cylinder 41 is fixedly connected to the outside of the steel wire plunger 12, an elastic T-shaped plastic cylinder 42 is fixedly connected to one end of the installation cylinder 41, there are four notches 43 equidistantly on the outside of the elastic T-shaped plastic cylinder 42, and a limiting cylinder 4 is slidably sleeved on the outside of the elastic T-shaped plastic cylinder 42. A spring 13 is sleeved on the outside of the steel wire plunger 12, and both ends of the spring 13 are respectively connected to the fixed cylinder 1 and the limiting cylinder 4. A conical sleeve 2 is slidably sleeved on the outside of the connecting cylinder 11, a needle 3 is arranged in the opening in the middle of the conical sleeve 2, connecting rods 44 are fixedly connected to both sides of the limiting cylinder 4, and the other ends of the two connecting rods 44 are respectively connected to both sides of the inner cavity of the conical sleeve 2. During work, by moving the conical sleeve 2 in the direction of the fixed cylinder 1, the limiting cylinder 4 is driven to approach the fixed cylinder 1 in cooperation with the connecting rod 44, and at the same time the spring 13 is compressed, so that the opening of the elastic T-shaped plastic cylinder 42 expands. Then, the needle 3 is inserted into the elastic T-shaped plastic cylinder 42 from the opening of the conical sleeve 2 and sleeved on the outside of the steel wire plunger 12. When the needle 3 moves to contact the installation cylinder 41 and cannot move, after the hand releases the conical sleeve 2, the elastic force of the spring 13 pushes the conical sleeve 2 to reset, and at the same time drives the limiting cylinder 4 to reset and sleeved on the outside of the elastic T-shaped plastic cylinder 42 to squeeze and tighten it to limit the needle 3. Therefore, the staff only needs to push the conical sleeve 2 and insert the needle 3 to complete the operation, which is convenient for the staff to operate quickly and improves the efficiency of fixing the needle 3.
[0020] Specifically, see Figure 2 , limiting grooves 5 are opened on both sides of the fixed cylinder 1, limiting blocks 51 are fixedly connected to both sides of the inner cavity of the conical sleeve 2, and the two limiting blocks 51 are respectively slidably connected in the two limiting grooves 5. Through the arrangement of the limiting grooves 5 and the limiting blocks 51, the limiting blocks 51 move in a guiding manner in the limiting grooves 5, so as to improve the stability of the movement of the conical sleeve 2 outside the fixed cylinder 1, and avoid the conical sleeve 2 separating from the fixed cylinder 1 and affecting the stability of fixing the needle 3.
[0021] Further, referring to Figure 1 , a plurality of anti-slip rings 21 are installed on the outer side of the conical sleeve 2, and the plurality of anti-slip rings 21 are equidistantly distributed on the outer side of the conical sleeve 2. The anti-slip rings 21 are used to increase the friction force when a person holds the conical sleeve 2 by hand, so as to facilitate the person to manually push the conical sleeve 2 to move for installing the needle 3.
[0022] It should be noted that, referring to Figure 2 , the spring 13 is always in a compressed state, and the elastic force of the spring 13 is greater than the frictional force between the limiting cylinder 4 and the elastic T-shaped plastic cylinder 42, so as to ensure that the limiting cylinder 4 can reset to squeeze and limit the elastic T-shaped plastic cylinder 42.
[0023] It should be noted that, referring to Figure 3 , a sealing gasket 14 is fixedly connected to the outer side of the wire plunger 12, and the sealing gasket 14 has elasticity, so as to improve the sealing performance of the connection between the needle 3 and the wire plunger 12.
[0024] In addition, the components designed in the present utility model are all common standard components or components known to those skilled in the art. Their structures and principles can all be known by those skilled in the art through technical manuals or through conventional experimental methods. Those skilled in the art can fully implement them without further elaboration. The content protected by the present utility model does not involve improvements to the internal structure and method either.
[0025] The embodiments disclosed in the present utility model are preferred embodiments, but not limited thereto. Those of ordinary skill in the art can easily understand the spirit of the present utility model according to the above embodiments and make different extensions and changes. However, as long as they do not depart from the spirit of the present utility model, they are all within the protection scope of the present utility model.
Claims
1. A fixing device for a microinjection needle tip for gene editing, comprising a fixing cylinder (1), characterized in that, One end of the fixed cylinder (1) is provided with a connecting cylinder (11) in the middle. One end of the fixed cylinder (1) near the connecting cylinder (11) is provided with a wire plunger (12) in the middle. An installation cylinder (41) is fixedly connected to the outside of the wire plunger (12). One end of the installation cylinder (41) is fixedly connected to an elastic T-shaped plastic cylinder (42). There are four notches (43) equidistantly arranged on the outside of the elastic T-shaped plastic cylinder (42). And a limiting cylinder (4) is slidably sleeved on the outside of the elastic T-shaped plastic cylinder (42). A spring (13) is sleeved on the outside of the wire plunger (12). The two ends of the spring (13) are respectively connected to the fixed cylinder (1) and the limiting cylinder (4). A conical sleeve (2) is slidably sleeved on the outside of the connecting cylinder (11). A needle (3) is arranged in the opening in the middle of the conical sleeve (2). Connecting rods (44) are fixedly connected to both sides of the limiting cylinder (4). The other ends of the two connecting rods (44) are respectively connected to both sides of the inner cavity of the conical sleeve (2).
2. The needle fixing device for gene editing by microinjection according to claim 1, characterized in that, Limiting grooves (5) are provided on both sides of the fixed cylinder (1). Limiting blocks (51) are fixedly connected to both sides of the inner cavity of the conical sleeve (2). The two limiting blocks (51) are respectively slidably connected in the two limiting grooves (5).
3. The needle fixing device for gene editing by microinjection according to claim 1, characterized in that, A plurality of anti-slip rings (21) are installed on the outside of the conical sleeve (2). And the plurality of anti-slip rings (21) are equidistantly distributed on the outside of the conical sleeve (2).
4. The needle fixing device for gene editing by microinjection according to claim 1, characterized in that, The spring (13) is always in a compressed state, and the elastic force of the spring (13) is greater than the friction force between the limiting cylinder (4) and the elastic T-shaped plastic cylinder (42).
5. The needle fixing device for gene editing by microinjection according to claim 1, characterized in that, A sealing gasket (14) is fixedly connected to the outside of the wire plunger (12), and the sealing gasket (14) has elasticity.