Cell counting test card

Abstract

The utility model discloses a cell counting test card which comprises a sample loading head and a handle, the sample loading head is a sheet-shaped piece made of a transparent material, the sheet-shaped piece is internally provided with a volume cavity for accommodating a sample to be detected, and one side of the volume cavity is provided with a sample adding port for adding the sample to be detected into the volume cavity; the handle is connected to the sample loading head and is used for being held and operated by a user; wherein the volume cavity comprises a measuring area and a storage area, the height size of the measuring area is smaller than that of the storage area, the storage area surrounds the periphery of the measuring area, and a to-be-detected sample in the storage area can flow into the measuring area. According to the cell counting test card disclosed by the utility model, the inner and outer surrounding structures of the storage area and the measurement area are utilized, and the height dimension difference of the storage area and the measurement area is utilized, so that a liquid sample can be fully filled in the measurement area, and the liquid sample is fully spread to achieve a more uniform and clear form, and further the accuracy and the reliability of a counting result are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to a cell counting test card. Background Art

[0002] In biomedical research and clinical laboratory applications, it is necessary to analyze cells to obtain corresponding information on basic research or physical signs. The commonly used instruments are flow cytometers and automatic hematology analyzers. In terms of function, both mainly focus on cell counting measurement (i.e., "counting") and type differentiation (i.e., "typing"). The previous methods all analyze single cells. Although accurate cell counting and typing can be achieved, there are problems such as expensive instruments, large volume, and professional operation, which are not suitable for cell analysis in on-site or non-special occasions. For example, in specific biomedical research, when it is not possible to bring the cell sample to be tested back to the laboratory and immediate on-site analysis is required, or in the case where community, family, village doctors, etc. need to perform blood cell analysis in other occasions such as non-inspection departments or central laboratories, it is not conducive to popularization and application.

[0003] Based on the above problems, in clinical laboratory applications, a disposable counting chip is used to load the sample, and a portable device is used for detection. The core structure of the counting chip is to process an open measuring cavity at the end of a transparent thin sheet. The stained liquid sample is added to the measuring cavity. In the measuring cavity, the cells spread along with the liquid, and then the light from the light source passes through the monolayer of cells in the measuring cavity and is imaged. Thus, relying on the color stain to achieve cell counting and the typing result based on cell morphology recognition can be obtained by the device for analysis. During this process, the structural design of the counting chip affects the effect of cell spreading, and thus has an important impact on the counting result. Summary of the Utility Model

[0004] The utility model aims to at least solve one of the technical problems in the related technologies to some extent. For this purpose, the purpose of the utility model is to propose a cell counting test card.

[0005] To achieve the above object, according to an embodiment of the utility model, the cell counting test card includes:

[0006] A sample loading head, which is a sheet made of a transparent material. There is a volume cavity for accommodating the sample to be detected inside the sheet, and a sample adding port for adding the sample to be detected into the volume cavity is provided on one side of the volume cavity;

[0007] A handle, which is connected to the sample loading head for the user to hold and operate;

[0008] Wherein, the volume chamber includes a measurement area and a storage area. The height dimension of the measurement area is smaller than that of the storage area. The storage area surrounds the periphery of the measurement area, and the sample to be detected in the storage area can flow into the measurement area.

[0009] According to the cell counting test card provided by the embodiment of the present invention, the volume chamber includes a measurement area and a storage area. The storage area has a large height dimension and surrounds the periphery of the measurement area. Thus, when adding a sample, the storage area can store a sufficient amount of liquid sample and flow into the measurement area. The measurement area has a small height dimension, which can fully spread the liquid sample to form a recognizable shape. That is, by using the inner and outer surrounding structure of the storage area and the measurement area, and using the height dimension difference between the two, it is ensured that the liquid sample can fully fill the measurement area and the liquid sample can be fully spread to achieve a more uniform and clear shape, thereby improving the accuracy and reliability of the counting result.

[0010] In addition, the cell counting test card according to the above embodiment of the present invention may further have the following additional technical features:

[0011] According to an embodiment of the present invention, the volume chamber has opposite top and bottom walls in the thickness direction of the sheet-like member;

[0012] The sample adding port is formed between the top and bottom walls and is located at one end of the sheet-like member away from the handle.

[0013] According to an embodiment of the present invention, the inner surface of the bottom wall has a boss protruding towards the top wall. There is a predetermined distance between the top surface of the boss and the inner surface of the top wall to define the measurement area.

[0014] According to an embodiment of the present invention, the inner surface of the bottom wall also has two drainage edges. The two drainage edges are respectively located on both sides of the boss and have the same height as the boss. A predetermined angle is formed between the two drainage edges. One end of each drainage edge is connected to the boss, and the other end of each drainage edge extends to the sample adding port.

[0015] According to an embodiment of the present invention, the sheet-like member is formed into a rectangular structure. One corner of the rectangular structure has a chamfer, and the sample adding port is formed at the position of the chamfer and extends to two adjacent sides of the chamfer.

[0016] According to an embodiment of the present invention, the side surface of the boss adjacent to the sample adding port is formed into a semi-circular shape.

[0017] According to an embodiment of the present invention, the width dimension of the handle is smaller than that of the sample loading head, so that a positioning step surface is formed at the connection between the handle and the sample loading head.

[0018] According to an embodiment of the present utility model, a positioning port for directionally positioning the cell counting test card when installing it is provided at one end of the handle away from the sample loading head.

[0019] According to an embodiment of the present utility model, a positioning protrusion and a positioning groove are provided at one end of the handle away from the sample loading head. The positioning protrusion is located on the top surface of the handle, and the positioning groove is located on the bottom surface of the handle and is opposite to the positioning protrusion.

[0020] According to an embodiment of the present utility model, the height dimension of the storage area is 0.2 to 1.0 mm, and the height dimension of the measurement area is 0.05 to 0.5 mm.

[0021] Additional aspects and advantages of the present utility model will be partially given in the following description, partially become apparent from the following description, or be understood through the practice of the present utility model. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or in the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on the structures shown in these drawings without creative efforts.

[0023] Figure 1 is a schematic structural diagram of a cell counting test card according to an embodiment of the present utility model;

[0024] Figure 2 is a schematic internal structure diagram of the sample loading head in the cell counting test card according to an embodiment of the present utility model;

[0025] Figure 3 is a side view of the cell counting test card according to an embodiment of the present utility model;

[0026] Figure 4 is Figure 3 a partial enlarged view of part A in

[0027] Reference numerals:

[0028] 10. Sample loading head;

[0029] 101. Top wall;

[0030] 102. Bottom wall;

[0031] 1021. Boss;

[0032] 1022. Drainage edge;

[0033] P10, Volume chamber;

[0034] P101, Storage area;

[0035] P102, Measurement area;

[0036] H10, Sampling port;

[0037] 20, Handle;

[0038] S201, Positioning step surface;

[0039] H201, Positioning port;

[0040] 201, Positioning protrusion.

[0041] The realization, functional features and advantages of the present utility model will be further described in conjunction with the embodiments with reference to the accompanying drawings. Detailed implementation manners

[0042] The embodiments of the present utility model will be described in detail below. The examples of the embodiments are shown in the accompanying drawings, in which the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the accompanying drawings are exemplary and are intended to explain the present utility model, and should not be construed as limiting the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the scope of protection of the present utility model.

[0043] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "circumferential", "radial", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

[0044] In addition, the terms "first" and "second" are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present utility model, "a plurality" means two or more unless otherwise specifically defined.

[0045] In the present utility model, unless otherwise clearly specified or limited, terms such as "installation", "connection", "linkage", "fixation" shall be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be a direct connection or an indirect connection through an intermediate medium, and it may be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0046] In the present utility model, unless otherwise clearly specified or limited, the first feature being "above" or "below" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through other features therebetween. Moreover, the first feature being "above", "over" and "on" the second feature includes that the first feature is directly above and obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "beneath" and "underneath" the second feature includes that the first feature is directly below and obliquely below the second feature, or merely indicates that the horizontal height of the first feature is lower than that of the second feature.

[0047] The cell counting test card according to the embodiment of the present utility model will be described in detail below with reference to the accompanying drawings.

[0048] Refer to Figures 1 to 4 As shown, the cell counting test card provided according to the embodiment of the present utility model includes a sample loading head 10 and a handle 20.

[0049] Specifically, the sample loading head 10 is a sheet-like member made of a transparent material, for example, it can be made of plexiglass, polycarbonate or other plastics with good light transmittance. A volume cavity P10 for accommodating the sample to be detected is provided inside the sheet-like member, and a sample adding port H10 for adding the sample to be detected into the volume cavity P10 is provided on one side of the volume cavity P10. The volume cavity P10 is used to accommodate the liquid sample to be detected. The sample adding port H10 on one side of the volume cavity P10 facilitates injecting the stained sample liquid drop into it. For example, the sample adding port H10 can be sampled by directly dipping through capillary action, or can be dropped into the sample adding port H10 through a dropper.

[0050] The handle 20 is connected to the sample loading head 10 for the user to hold and operate. The material of the handle 20 can be selected to be the same as that of the sample loading head 10 to form an integral structure. Of course, different materials can be used and fixed together by fixing methods such as plugging and clamping. The handle 20 is used as an operation handle, which is convenient for holding the cell counting test card.

[0051] The volume chamber P10 includes a measurement area P102 and a storage area P101. The height dimension of the measurement area P102 is smaller than that of the storage area P101. The storage area P101 surrounds the periphery of the measurement area P102, and the sample to be detected in the storage area P101 can flow into the measurement area P102.

[0052] That is to say, the volume chamber P10 is mainly composed of two parts: a measurement area P102 and a storage area P101. The measurement area P102 is located in the center of the volume chamber P10 and is a flat chamber with a small height. The storage area P101 surrounds the periphery of the measurement area P102 and has an encircling structure, and its height dimension is larger than that of the measurement area P102. The two areas are interconnected, enabling the liquid sample in the storage area P101 to slowly flow into the measurement area P102.

[0053] The key to the structural design of this volume chamber P10 lies in making use of the difference in height dimensions between the measurement area P102 and the storage area P101. During use, the operator first adds the sample to be detected into the storage area P101 through the sample addition port H10. The storage area P101 has a large volume and can hold a sufficient amount of sample liquid. Subsequently, the liquid sample in the storage area P101 slowly flows into the measurement area P102 under the action of gravity. Since the height dimension of the measurement area P102 is very small, the sample will naturally spread out to form an extremely thin liquid layer therein. When the light from the light source passes through the measurement area P102 and then enters the image sensor for imaging, the detection device can analyze and process the image to obtain the cell counting result. In this way, the design of the above-mentioned volume chamber P10 makes the distribution of the liquid sample in the measurement area P102 more uniform, the layer thickness thinner, and the arrangement of cells more dispersed. At the same time, since the measurement area P102 has a flat structure, the cells therein are arranged in a single layer, and the morphological distribution of the cells is clearer and more distinguishable, avoiding the analysis interference caused by cell overlap, thereby improving the accuracy and reliability of cell counting.

[0054] According to the cell counting test card provided by the embodiment of the present invention, the volume chamber P10 includes a measurement area P102 and a storage area P101. The storage area P101 has a large height dimension and surrounds the periphery of the measurement area P102. In this way, when adding samples, the storage area P101 can be used to store a sufficient amount of liquid sample and flow into the measurement area P102. The measurement area P102 has a small height dimension and can fully spread out the liquid sample to form a recognizable morphology. That is to say, by using the inner and outer surrounding structure of the storage area P101 and the measurement area P102 and making use of the height dimension difference between the two, it is ensured that the liquid sample can fully fill the measurement area P102 and the liquid sample can be fully spread out to achieve a more uniform and clear morphology, thereby improving the accuracy and reliability of the counting result.

[0055] In some embodiments of the present utility model, the volume chamber P10 has an opposite top wall 101 and bottom wall 102 in the thickness direction of the sheet-like member; a sample addition port H10 is formed between the top wall 101 and the bottom wall 102 and is located at one end of the sheet-like member away from the handle 20.

[0056] The volume chamber P10 defined by such a top wall 101 and bottom wall 102 is conducive to the imaging requirements of the light source from top to bottom or from bottom to top during detection. The position of the sample addition port H10 is at one end away from the handle 20 and on the side of the volume chamber P10, enabling the operator to directly drip the liquid sample to be tested onto the sample addition port H10 during sample addition, or directly insert the sample addition port H10 into the sample for dipping, facilitating the addition of the sample.

[0057] That is to say, with the volume chamber P10 having the above structure, it not only ensures that the sample liquid can smoothly flow into the volume chamber P10 and spread evenly, but also facilitates the optical imaging detection during detection.

[0058] In an embodiment of the present utility model, the inner surface of the bottom wall 102 has a boss 1021 protruding towards the top wall 101. There is a predetermined distance between the top surface of the boss 1021 and the inner surface of the top wall 101 to define the measurement area P102. The material of the boss 1021 is usually the same as that of the sample carrier head 10 body, such as transparent plastics like polycarbonate, to ensure that the light transmittance for subsequent imaging is not affected.

[0059] By providing the boss 1021, the height dimension of the measurement area P102 is smaller than the height dimension of the storage area P101, and the height dimension of the measurement area P102 is equal to the distance dimension between the top surface of the boss 1021 and the top wall 101 of the volume chamber P10. In addition, the height dimension of the storage area P101 outside the measurement area P102 is equal to the distance dimension between the top wall 101 and the bottom wall 102 of the container chamber, and there is an overlapping part between the storage area P101 and the measurement area P102 within the height range. It is precisely this overlapping part that directly connects the two. In this way, the sample liquid is more likely to flow from the storage area P101 into the measurement area P102 and spread to form a liquid layer within the measurement area P102.

[0060] In an embodiment of the present utility model, the inner surface of the bottom wall 102 also has two drainage edges 1022. The two drainage edges 1022 are respectively located on both sides of the boss 1021 and have the same height as the boss 1021. A predetermined angle is formed between the two drainage edges 1022. One end of each drainage edge 1022 is connected to the boss 1021, and the other end of each drainage edge 1022 extends to the sample addition port H10.

[0061] In this embodiment, drainage edges 1022 are provided on both sides of the boss 1021, and the purpose is to use the drainage platform to guide the flow path of the liquid sample within the volume chamber P10. When an operator drips the liquid sample onto the sampling port H10, the drainage edges 1022 can effectively guide the liquid onto the boss 1021, and spread the liquid sample within the measurement area P102 through the thin gap between the boss 1021 and the top wall 101 of the volume chamber P10.

[0062] Since the height dimension of the measurement area P102 is relatively small, when the liquid sample enters the measurement area P102 from the storage area P101, it may be affected by factors such as the surface tension of the liquid and it is difficult to smoothly enter the measurement area P102. There may be unfilled and unspread areas such as air bubbles in the measurement area P102. In this embodiment, by providing the drainage edges 1022 on both sides of the boss 1021, the liquid sample can be guided to enter the measurement area P102 more smoothly, making it easier for the measurement area P102 to be flattened and filled, further improving and ensuring the liquid layer uniformity of the measurement area P102, and thus improving the accuracy of the measurement results.

[0063] In some embodiments of the present utility model, the sheet-shaped member is formed into a rectangular structure, and one corner of the rectangular structure has a chamfer. The sampling port H10 is formed at the position of the chamfer and extends to two adjacent sides of the chamfer. This chamfer can be a right chamfer or an arc chamfer.

[0064] In this embodiment, the chamfer structure is utilized to open the sampling port H10 of the volume chamber P10 at this chamfer. Moreover, the range of the sampling port H10 not only covers the chamfer area but also extends to partial areas of two straight sides adjacent to this chamfer. Thus, the shape of the sampling port H10 presents a fan-shaped structure.

[0065] This fan-shaped sampling port H10 has a larger coverage range, and the measurement area P102 is located at the middle position of the fan-shaped structure, forming an enclosed structure, making it easier to inject the liquid sample. At the same time, the fan-shaped structure enables the entered liquid to radiate and diffuse evenly in all directions, which is beneficial to evenly covering the entire area of the measurement area P102, thereby further improving the accuracy and consistency of the test results.

[0066] Preferably, the side surface of the boss 1021 adjacent to the sampling port H10 is formed into a semi-circular shape. This semi-circular structure is conducive to the liquid sample in the storage area P101 entering the measurement area P102 more smoothly. At the same time, it can also have better adaptability with the light source, ensuring that the light of the light source can cover the entire measurement area P102, achieving a more comprehensive and accurate detection.

[0067] In an embodiment of the present utility model, the width dimension of the handle 20 is smaller than the width dimension of the sample carrier head 10, so as to form a positioning step surface S201 at the connection between the handle 20 and the sample carrier head 10. In specific applications, there is a card slot in the detection device for placing the cell counting test card. By setting the positioning step surface S201, it is convenient to position and fix the test card, ensuring the stability of the test card in the card slot.

[0068] Advantageously, a positioning port H201 for directionally positioning the cell counting test card during its installation is provided at one end of the handle 20 away from the sample carrier head 10. The positioning port H201 is adapted to the structure of the card slot. When installing the test card, the positioning port H201 can ensure that the front side of the test card is placed upward, which is beneficial to the rapid loading of the test card during the test and makes it more convenient to use.

[0069] In some embodiments of the present utility model, a positioning protrusion 201 and a positioning groove are provided at one end of the handle 20 away from the sample carrier head 10. The positioning protrusion 201 is located on the top surface of the handle 20, and the positioning groove is located on the bottom surface of the handle 20 and is opposite to the positioning protrusion 201. In specific applications, when multiple test cards are stacked, the positioning groove at the bottom of the upper test card is in positioning cooperation with the positioning protrusion 201 at the top of the lower test card. In this way, it is convenient to stack multiple test cards.

[0070] In some embodiments of the present utility model, the height dimension of the storage area P101 is 0.2 to 1.0 mm, and the height dimension of the measurement area P102 is 0.05 to 0.5 mm. This height dimension is involved to ensure that the liquid sample has a sufficient amount and can be more fully developed in the measurement area P102, forming a clearer liquid layer morphology.

[0071] In some embodiments of the present utility model, a unique information identification code is provided on the surface of the handle. The information identification code can be a one-dimensional code or a two-dimensional code. During the detection process, by scanning the information identification code with a scanning device, reagent and / or detection information, etc. can be obtained. In this way, it is beneficial to data statistics and management.

[0072] In the description of this specification, the descriptions referring to terms such as "one embodiment", "some embodiments", "examples", "specific examples", or "some examples", etc. mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0073] The above are only the preferred embodiments of the present utility model, and do not limit the patent scope of the present utility model. Any equivalent structural transformation made by using the content of the specification and drawings of the present utility model under the inventive concept of the present utility model, or direct / indirect application in other related technical fields is included in the patent protection scope of the present utility model.

Claims

1. A cell counting test card, characterized in that, Comprising: A sample loading head, which is a sheet made of a transparent material. There is a volume cavity for accommodating a sample to be detected inside the sheet. One side of the volume cavity has a sample adding port for adding the sample to be detected into the volume cavity. A handle, which is connected to the sample loading head for the user to hold and operate. Among them, the volume cavity includes a measurement area and a storage area. The height dimension of the measurement area is smaller than that of the storage area. The storage area surrounds the measurement area on the periphery, and the sample to be detected in the storage area can flow into the measurement area.

2. The cell counting test card according to claim 1, wherein The volume cavity has an opposite top wall and bottom wall in the thickness direction of the sheet. The sample adding port is formed between the top wall and the bottom wall and is located at one end of the sheet away from the handle.

3. The cell counting test card according to claim 2, wherein The inner surface of the bottom wall has a convex platform protruding towards the top wall. There is a predetermined distance between the top surface of the convex platform and the inner surface of the top wall to define the measurement area.

4. The cell counting test card according to claim 3, wherein The inner surface of the bottom wall also has two drainage edges. The two drainage edges are respectively located on both sides of the convex platform and have the same height as the convex platform. A predetermined angle is formed between the two drainage edges. One end of each drainage edge is connected to the convex platform, and the other end of each drainage edge extends to the sample adding port.

5. The cell counting test card according to claim 1, wherein The sheet is formed into a rectangular structure. One corner of the rectangular structure has a chamfer. The sample adding port is formed at the position of the chamfer and extends to two adjacent sides of the chamfer.

6. The cell counting test card according to claim 4, wherein, The side surface of the convex platform adjacent to the sample adding port is formed into a semi-circular shape.

7. The cell counting test card according to claim 1, wherein The width dimension of the handle is smaller than that of the sample loading head, so that a positioning step surface is formed at the connection between the handle and the sample loading head.

8. The cell counting test card according to claim 1, wherein The surface of the handle is provided with a unique information identification code; one end of the handle away from the sample loading head is provided with a positioning port for directionally positioning the cell counting test card when installing it.

9. The cell counting test card according to claim 1, wherein One end of the handle away from the sample loading head is provided with a positioning protrusion and a positioning groove. The positioning protrusion is located on the top surface of the handle, and the positioning groove is located on the bottom surface of the handle and is opposite to the positioning protrusion.

10. The cell counting test card according to claim 1, characterized in that, The height dimension of the storage area is 0.2 to 1.0 mm, and the height dimension of the measurement area is 0.05 to 0.5 mm.

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