Detection kit for protein detection

Abstract

The utility model discloses a detection kit for protein detection, which comprises a main body structure and a transfer membrane structure, the transfer membrane structure is detachably inserted in the main body structure, and the detection kit for protein detection relates to the technical field of microbiological detection equipment. The method can be realized only by adopting a trace amount of detection liquid and reagent, the overall detection cost is low, harmful liquid is prevented from harming personnel through sealing of the detection box, meanwhile, the detection box is small in size and portable, field analysis can be carried out, other parts of the kit except for sample adding do not need to be manually operated and are automatically completed (internal setting is preset), and the detection accuracy is high. The method has the advantage of small sample consumption in use.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial detection equipment, in particular to a detection kit for protein detection. Background Art

[0002] Immunoblotting, also known as protein blotting, is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody.

[0003] Protein determination refers to the determination of protein content by physical or chemical methods. Proteins are important components of human cells and tissues, and protein determination is one of the most commonly used and basic analytical methods in biochemistry and molecular biology research.

[0004] In the prior art, the microfluidic chip technology is mostly adopted, which is a science and technology mainly characterized by manipulating fluids in a micron-scale space. At present, the mainstream form of microfluidic chip refers to integrating or basically integrating basic operation units such as sample preparation, reaction, separation, detection, cell culture, sorting, and lysis involved in the fields of chemistry and biology onto a chip of a few square centimeters or even smaller. A network is formed by microchannels, and a controllable fluid runs through the whole system to realize various functions of different laboratories such as conventional chemistry, biology, materials, and optics. Therefore, if the protein blotting detection is concentrated on the microfluidic chip, it will simplify the manual operation steps, improve the accuracy of detection data, and there are some reagents with strong toxicity in the experiments, which pose a threat to the health of researchers. Therefore, a detection kit for protein detection is designed now. Content of the Utility Model

[0005] The purpose of the utility model is to provide a detection kit for protein detection to solve the problems of preventing personal injury in experiments and simplifying the experimental steps and improving the accuracy of experimental data.

[0006] To achieve the above object, the utility model provides the following technical solution: A detection kit for protein detection, comprising a main body structure and a membrane transfer structure, and the membrane transfer structure is detachably inserted into the main body structure.

[0007] Preferably, the main body structure includes an inspection box, a pair of electrophoresis electrode plates, a liquid injection unit, a liquid pushing unit, and a microfluidic chip.

[0008] The test kit is a rectangular box body, and a socket is provided in the middle of the right side wall thereof. A pair of electrophoresis electrode plates are symmetrically arranged on the front and rear side walls of the test kit respectively and are located at the right end. A pair of electrophoresis electrode plates are respectively connected to the positive electrode and the negative electrode of a power supply. The liquid injection unit is fixedly arranged on the right side wall inside the test kit and is located above one of the electrophoresis electrode plates. The liquid pushing unit is fixedly arranged on the lower wall inside the test kit, is located on the left side of the liquid injection unit and is connected to the liquid injection unit. The microfluidic chip is fixedly arranged on the lower wall inside the test kit and is located in front of the liquid pushing unit. The microfluidic chip faces the socket.

[0009] Preferably, the liquid injection unit includes a liquid injection cylinder, a piston, a piston rod, a pair of solenoid valves, a first electric push rod and a connecting rod;

[0010] One end of the liquid injection cylinder is fixedly penetrated through the right side wall of the test kit. The piston is movably installed in the liquid injection cylinder. One end of the piston rod is fixedly connected to the middle of the piston, and the other end of the piston rod is located on the right side of the test kit. One ends of a pair of solenoid valves are respectively fixedly arranged on the left side wall and the upper wall at the other end of the liquid injection cylinder. The other end of one of the solenoid valves is fixedly penetrated through the upper wall of the test kit, and the other end of the other solenoid valve is arranged downward. The first electric push rod is fixedly arranged on the upper wall of the test kit and is close to the rear end. Both ends of the connecting rod are connected to the telescopic end of the first electric push rod and the other end of the piston rod respectively.

[0011] Preferably, the liquid pushing unit includes a reaction box, a feeding tube, a tube cap, a second electric push rod, a pushing plate and a lead-out tube;

[0012] The reaction box is a rectangular box body, and first legs are arranged at the four corners of the lower wall. The reaction box is fixedly arranged on the lower wall inside the test kit through the first legs and is located on the left side of the liquid injection cylinder. The upper wall of the reaction box is connected to the other end of the other solenoid valve, and a lead-out port is arranged at a position near the top in the middle of the right side wall of the reaction box. One end of the feeding tube is fixedly connected to the upper wall of the reaction box, and the other end of the feeding tube is fixedly penetrated through the upper wall of the test kit. The tube cap is detachably sleeved on the other end of the feeding tube. One end of the second electric push rod is fixedly arranged on the lower wall inside the test kit and is opposite to the middle of the lower wall of the reaction box. The telescopic end of the second electric push rod can penetrate through the middle of the lower wall of the reaction box. The pushing plate is movably installed in the reaction box, and the pushing plate is fixedly connected to the telescopic end of the second electric push rod. The pushing plate fits with the inner wall of the reaction box. One end of the lead-out tube is fixedly arranged on the right side wall of the reaction box and is located at the position of the lead-out port. The other end of the lead-out tube is located in front of the reaction box and is located above the microfluidic chip.

[0013] Preferably, the membrane transfer structure includes an adjusting frame, a pair of squeezing frames, two pairs of springs, a rubber plate and a pair of membrane transfer electrode plates;

[0014] The adjusting frame is a rectangular cavity structure, and a pair of sliding grooves are symmetrically arranged on the left side wall of the adjusting frame near the upper and lower ends, and the upper and lower side walls of the adjusting frame are provided with telescopic openings corresponding to the sliding grooves. A pair of the extrusion frames are both concave structures, and the width of one of the extrusion frames is smaller than the width of the other extrusion frame. Both ends of the pair of extrusion frames are movably passed through the telescopic openings and are located in the adjusting frame. A pair of the extrusion frames are provided with a protrusion in the middle of one end, and the protrusions are movably embedded in the sliding grooves respectively. The width of one end of the pair of extrusion frames is greater than the width of the slide groove, and one end of the extrusion frames respectively blocks and seals the slide groove. One end of the two pairs of springs is respectively fixed on the two ends of the extrusion frames, and the springs are respectively located between the two ends of the extrusion frames and the inner side walls of the adjusting frame and can be compressed by force. The rubber plate is detachably arranged in the middle of the left side wall of the adjusting frame and is located between the two pairs of slide grooves. A pair of transfer electrode plates are respectively detachably arranged on the protrusions at both ends of the extrusion frame, and the transfer electrode plates are respectively located symmetrically on the upper and lower sides of the rubber plate.

[0015] Preferably, the gel plate and the transfer electrode plate are detachably inserted into the test box, respectively, and the gel plate is relatively connected and fit with the microfluidic chip.

[0016] Preferably, the adjustment frame is penetrated through four corners by two pairs of mounting bolts and is screwed and sealed to the inspection box.

[0017] Compared with the prior art, the utility model has the following beneficial effects: the detection kit for protein detection can be realized by only using a trace amount of detection liquid and reagents, and the overall detection cost is low. The sealing of the detection box prevents harmful liquids from causing harm to personnel. At the same time, the detection box is small and portable and can be analyzed on-site. Except for adding samples, the rest of the test kit does not require manual operation and is automatically completed (its internal settings are all pre-set), and it has the advantage of small sample consumption when used. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the appearance structure of the utility model;

[0019] Figure 2 It is a partial enlarged structural schematic diagram of A of the utility model;

[0020] Figure 3 This is a schematic diagram of the perspective split structure of the main structure of the utility model;

[0021] Figure 4 This is a perspective assembly structure diagram of the main structure of the utility model;

[0022] Figure 5 This is a schematic diagram of the split structure of the transfer membrane structure of the utility model;

[0023] Figure 6 It is a schematic diagram of the assembly structure of the transfer membrane structure of the present utility model.

[0024] In the figure: 1. Main body structure; 11. Inspection box; 12. Electrophoresis electrode plate; 13. Liquid injection unit, including 131. Liquid injection cylinder, 132. Piston, 133. Piston rod, 134. Solenoid valve, 135. First electric push rod, 136. Connecting rod; 14. Liquid pushing unit, including 141. Reaction box, 142. Feeding tube, 143. Tube cap, 144. Second electric push rod, 145. Pushing plate, 146. Discharge tube; 15. Microfluidic chip; 2. Membrane transfer structure, including 21. Adjusting frame, 22. Extrusion frame, 23. Spring, 24. Rubber plate, 25. Membrane transfer electrode plate; 3. Chute; 4. Convex block; 6. Installation bolt. Specific embodiments

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0026] Please refer to Figures 1-6 , the present invention provides a technical solution: a detection kit for protein detection, including a main body structure 1 and a membrane transfer structure 2, and the membrane transfer structure 2 is detachably inserted into the main body structure 1.

[0027] The following are the model numbers and functions of the electrical components in this case:

[0028] Microfluidic chip: It is a prior art, and any microfluidic chip applicable to this solution can be used.

[0029] First electric push rod: It is a prior art, and any electric push rod applicable to this solution can be used

[0030] Second electric push rod: It is a prior art, and any electric push rod applicable to this solution can be used.

[0031] Microfluidic chip: It adopts the prior art, and any microfluidic chip applicable to this solution and having an integrated unit for protein blotting detection steps can be used.

[0032] As a preferred solution, further, the main body structure 1 includes an inspection box 11, a pair of electrophoresis electrode plates 12, a liquid injection unit 13, a liquid pushing unit 14, and a microfluidic chip 15;

[0033] The test cartridge 11 is a rectangular box body, and a socket is provided in the middle of its right side wall. A pair of electrophoresis electrode plates 12 are symmetrically arranged on the front and rear side walls of the test cartridge 11 respectively, and are located at the right end. A pair of electrophoresis electrode plates 12 are respectively connected to the positive electrode and the negative electrode of a power supply. The liquid injection unit 13 is fixedly arranged on the right side wall inside the test cartridge 11 and is located above one of the electrophoresis electrode plates 12. The liquid pushing unit 14 is fixedly arranged on the lower wall inside the test cartridge 11, is located on the left side of the liquid injection unit 13 and is connected to the liquid injection unit 13. The microfluidic chip 15 is fixedly arranged on the lower wall inside the test cartridge 11 and is located in front of the liquid pushing unit 14. The microfluidic chip 15 faces the socket. The liquid injection unit 14 facilitates the storage and micro supply of the lysis solution, and the liquid pushing unit 15 facilitates the transportation of the decomposed protein.

[0034] As a preferred solution, furthermore, the liquid injection unit 13 includes a liquid injection cylinder 131, a piston 132, a piston rod 132, a pair of electromagnetic valves 134, a first electric push rod 135 and a connecting rod 136;

[0035] One end of the liquid injection cylinder 131 is fixedly penetrated through the right side wall of the test cartridge 11. The piston 132 is movably installed in the liquid injection cylinder 131. One end of the piston rod 132 is fixedly connected to the middle of the piston 132, and the other end of the piston rod 132 is located on the right side of the test cartridge 11. One ends of a pair of electromagnetic valves 134 are respectively fixedly arranged on the left side wall and the upper wall at the other end of the liquid injection cylinder 131. The other end of one of the electromagnetic valves 134 is fixedly penetrated through the upper wall of the test cartridge 11, and the other end of the other electromagnetic valve 134 is arranged downward. The first electric push rod 135 is fixedly arranged on the upper wall of the test cartridge 11 and is close to the rear end. The two ends of the connecting rod 136 are respectively connected to the telescopic end of the first electric push rod 135 and the other end of the piston rod 132.

[0036] As a preferred solution, furthermore, the liquid pushing unit 14 includes a reaction box 141, a feeding tube 142, a tube cap 143, a second electric push rod 144, a pushing plate and a guiding tube 146;

[0037] The reaction box 141 is a rectangular box, and the four corners of the lower wall are all provided with first legs. The reaction box 141 is fixedly arranged on the lower wall of the test box 11 through the first legs, and is located on the left side of the injection cylinder 131. The upper wall of the reaction box 141 is connected to the other end of another electromagnetic valve 134, and a guide outlet is arranged near the top of the middle of the right side wall of the reaction box 141. One end of the delivery tube 142 is fixedly connected to the upper wall of the reaction box 141, and the other end of the delivery tube 142 is fixedly passed through the upper wall of the test box 11. The tube cap 143 is detachably mounted on the other end of the delivery tube 142. The second electric push rod One end of 144 is fixedly set on the lower wall of the test box 11 and is opposite to the middle part of the lower wall of the reaction box 141. The telescopic end of the second electric push rod 144 can pass through the middle part of the lower wall of the reaction box 141. The pushing plate 145 is movably embedded in the reaction box 141, and the pushing plate 145 is fixedly connected to the telescopic end of the second electric push rod 144. The pushing plate 145 fits with the inner wall of the reaction box 141. One end of the export tube 146 is fixedly set on the right side wall of the reaction box 141 and is located at the export port. The other end of the export tube 146 is located on the front side of the reaction box 141 and above the microfluidic chip 15.

[0038] As a preferred solution, further, the transfer film structure 2 includes an adjustment frame 21, a pair of extrusion frames 22, two pairs of springs 23, a rubber plate 24 and a pair of transfer film electrode plates 25;

[0039] The adjusting frame 21 is a rectangular cavity structure, and a pair of sliding grooves are symmetrically arranged on the left side wall of the adjusting frame 21 near the upper and lower ends, and the upper and lower side walls of the adjusting frame 21 are provided with telescopic openings corresponding to the sliding grooves. The pair of extrusion frames 22 are both concave structures, and the width of one extrusion frame 22 is smaller than the width of the other extrusion frame 22. The two ends of the pair of extrusion frames 22 are respectively movably passed through the telescopic openings and are located in the adjusting frame 21. The middle part of one end of the pair of extrusion frames 22 is provided with a protrusion 4, and the protrusion 4 is respectively movably embedded in the sliding groove. The width of one end of the pressing frame 22 is greater than the width of the slide groove 3, and one end of the pressing frame 22 blocks and seals the slide groove 3 respectively. One ends of the two pairs of springs 23 are fixedly arranged on the two ends of the pressing frame 22 respectively, and the springs 23 are respectively located between the two ends of the pressing frame 22 and the inner walls of the adjusting frame 21 and can be compressed by force. The rubber plate 24 is detachably arranged in the middle of the left side wall of the adjusting frame 21 and is located between the two pairs of slide grooves 3. A pair of transfer electrode plates 25 are detachably arranged on the protrusions 4 at the two ends of the pressing frame 22 respectively, and the transfer electrode plates 25 are respectively located symmetrically on the upper and lower sides of the rubber plate 24.

[0040] As a preferred solution, further, the gel plate 24 and the transfer electrode plate 25 are detachably inserted into the test box 11, and the gel plate 24 is connected and matched with the microfluidic chip 15 to achieve electrophoresis and produce an electrophoresis pattern according to design requirements.

[0041] As a preferred solution, further, the adjustment frame 21 is hermetically fixed by two pairs of mounting bolts 6 passing through the four corner parts and screwed to the inspection box 11, which is convenient for disassembly and replacement according to the design requirements.

[0042] The detailed connection means are well-known techniques in the art. The following mainly introduces the working principle and process, and the specific work is as follows.

[0043] Example: Through the attached Figures 1-6 It can be seen that:

[0044] First, before use, the first electric push rod 135 in the liquid injection unit 13 is contracted, the piston rod 132 is driven by the connecting rod 136 to insert into the liquid injection cylinder 131, and the piston 132 is driven to move hermetically close to the solenoid valve 134; then one of the solenoid valves 134 is opened to connect and extract the lysis solution, and the other solenoid valve 134 is closed during the extraction process;

[0045] After extracting the lysis solution, the two solenoid valves 134 can be interchanged and controlled to close, and the lysis solution can be pushed by the piston 132 into the reaction box 141 in the liquid pushing unit 14 and located on the pushing plate; then the tube cap 143 on the upper wall of the inspection box 11 in the main body structure 1 is opened, and the sample enters the reaction box 141 through the feeding tube 142 to react with the lysis solution to release proteins from the cells (note: the liquid level should be lower than the export at this time); Lysis is to add detergents such as SDS to destroy the cell structure, and after standing still, DNA forms a precipitate, and proteins are stored in the supernatant;

[0046] Secondly, control the second electric push rod 144 to drive the pushing plate 145 to rise, so that the overall liquid level in the reaction box 141 rises, and the floating proteins pass through the export and flow to the microfluidic chip 15 through the export tube 146 for detection. In the chip, the proteins in the supernatant enter the gel plate 24 through the channel, and the electrophoresis electrode plates 12 on the front and back sides are energized for electrophoresis to produce an electrophoresis pattern;

[0047] Finally, by manually pressing the extrusion frames 22 on the upper and lower sides of the adjustment frame 21 in the membrane transfer structure 2 to apply a relative movement force, the extrusion frames 22 will forcefully compress the springs 23, and move relatively in the chute 3 by means of the convex blocks 4, driving the pre-installed membrane transfer electrode plates 25 to move relatively and clamp on both sides of the gel plate 24. After generating the membrane transfer product by the semi-dry method, the mounting bolts 6 can be loosened, the adjustment frame 21 can be detached from the inspection box 11, and the gel plate 24 and the membrane transfer electrode plates 25 can be taken out.

[0048] Although embodiments of the present utility model have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present utility model. The scope of the present utility model is defined by the appended claims and their equivalents.

Claims

1. A detection kit for protein detection, characterized in that: It includes a main body structure (1) and a membrane transfer structure (2), and the membrane transfer structure (2) is detachably inserted into the main body structure (1); The main body structure (1) includes an inspection box (11), a pair of electrophoresis electrode plates (12), a liquid injection unit (13), a liquid pushing unit (14), and a microfluidic chip (15); The inspection box (11) is a rectangular box body, and a socket is provided in the middle of the right side wall thereof. A pair of the electrophoresis electrode plates (12) are symmetrically arranged on the front and rear side walls of the inspection box (11) and are located at the right end. A pair of the electrophoresis electrode plates (12) are respectively connected to the positive electrode and the negative electrode of a power supply. The liquid injection unit (13) is fixedly arranged on the right side wall inside the inspection box (11) and is located above one of the electrophoresis electrode plates (12). The liquid pushing unit (14) is fixedly arranged on the lower wall inside the inspection box (11), is located on the left side of the liquid injection unit (13) and is connected to the liquid injection unit (13). The microfluidic chip (15) is fixedly arranged on the lower wall inside the inspection box (11) and is located on the front side of the liquid pushing unit (14). The microfluidic chip (15) faces the socket.

2. The detection kit for protein detection according to claim 1, wherein: The liquid injection unit (13) includes a liquid injection cylinder (131), a piston (132), a piston (132) rod, a pair of solenoid valves (134), a first electric push rod (135), and a connecting rod (136); One end of the liquid injection cylinder (131) is fixedly penetrated through the right side wall of the inspection box (11). The piston (132) is movably embedded in the liquid injection cylinder (131). One end of the piston (132) rod is fixedly connected to the middle of the piston (132), and the other end of the piston (132) rod is located on the right side of the inspection box (11). One end of a pair of the solenoid valves (134) is respectively fixedly arranged on the left side wall and the upper wall at the other end of the liquid injection cylinder (131). The other end of one of the solenoid valves (134) is fixedly penetrated through the upper wall of the inspection box (11), and the other end of the other solenoid valve (134) is arranged downward. The first electric push rod (135) is fixedly arranged on the upper wall of the inspection box (11) and is close to the rear end. The two ends of the connecting rod (136) are respectively connected to the telescopic end of the first electric push rod (135) and the other end of the piston (132) rod.

3. The detection kit for protein detection according to claim 2, wherein: The liquid pushing unit (14) includes a reaction box (141), a delivery pipe (142), a pipe cap (143), a second electric push rod (144), a pushing plate (145), and a lead-out pipe (146); The reaction box (141) is a rectangular box body, and first legs are provided at the four corners of the lower wall. The reaction box (141) is fixedly arranged on the inner lower wall of the test box (11) through the first legs and is located on the left side of the liquid injection cylinder (131). The upper wall of the reaction box (141) is connected to the other end of another electromagnetic valve (134), and a lead-out port is arranged at a position near the top in the middle of the right side wall of the reaction box (141). One end of the feeding tube (142) is fixedly connected to the upper wall of the reaction box (141), and the other end of the feeding tube (142) fixedly penetrates through the upper wall of the test box (11). The tube cap (143) is detachably sleeved on the other end of the feeding tube (142). One end of the second electric push rod (144) is fixedly arranged on the inner lower wall of the test box (11) and is opposite to the middle of the lower wall of the reaction box (141). The telescopic end of the second electric push rod (144) can penetrate through the middle of the lower wall of the reaction box (141). The pushing plate (145) is movably embedded in the reaction box (141), and the pushing plate (145) is fixedly connected to the telescopic end of the second electric push rod (144). The pushing plate (145) fits with the inner wall of the reaction box (141). One end of the lead-out tube (146) is fixedly arranged on the right side wall of the reaction box (141) and is located at the lead-out port position. The other end of the lead-out tube (146) is located in front of the reaction box (141) and above the microfluidic chip (15).

4. The detection kit for protein detection according to claim 3, characterized in that: The membrane transfer structure (2) includes an adjusting frame (21), a pair of pressing frames (22), two pairs of springs (23), a rubber plate (24), and a pair of membrane transfer electrode plates (25); The adjusting frame (21) is a rectangular cavity structure, and a pair of sliding grooves are symmetrically arranged near the upper and lower ends of the left side wall of the adjusting frame (21). The upper and lower side walls of the adjusting frame (21) are provided with telescopic openings corresponding to the sliding grooves. Both of the pair of pressing frames (22) are concave structures, and the width of one of the pressing frames (22) is smaller than that of the other pressing frame (22). The two ends of the pair of pressing frames (22) respectively penetrate through the telescopic openings and are located inside the adjusting frame (21). A convex block (4) is arranged in the middle of one end of each of the pair of pressing frames (22), and the convex blocks (4) are respectively movably embedded in the sliding grooves. The width of one end of each of the pair of pressing frames (22) is larger than the width of the sliding groove (3), and one end of the pressing frame (22) respectively blocks and seals the sliding groove (3). One end of each of the two pairs of springs (23) is respectively fixedly arranged on the two ends of the pressing frame (22), and the springs (23) can be compressed under force between the two ends of the pressing frame (22) and the inner side wall of the adjusting frame (21). The rubber plate (24) is detachably arranged in the middle of the left side wall of the adjusting frame (21) and is located between the two pairs of sliding grooves (3). A pair of membrane transfer electrode plates (25) are respectively detachably arranged on the convex blocks (4) at the two ends of the pressing frame (22), and the membrane transfer electrode plates (25) are symmetrically arranged on the upper and lower sides of the rubber plate (24).

5. The detection kit for protein detection according to claim 4, wherein: The glue plate (24) and the transfer electrode plate (25) are respectively detachably inserted into the test box (11), and the glue plate (24) is relatively connected and matched with the microfluidic chip (15).

6. The detection kit for protein detection according to claim 5, characterized in that: The adjustment frame (21) is penetrated through four corners by two pairs of mounting bolts (6) and is screwed and sealed to the inspection box (11).

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