Chromatographic separation and collection device

Abstract

The utility model discloses a chromatographic separation collecting device, particularly relates to the technical field of chromatographic separation, and comprises a containing pipe, a connecting pipe and a connecting column, the top of the containing pipe is provided with the connecting pipe, the bottom of the connecting pipe is provided with the connecting column, the inner center of the connecting pipe is vertically provided with a sleeve, the bottom of the sleeve is provided with a communicating piece, and the communicating piece is connected with the connecting pipe. Extrusion rings are mounted at the tops of the accommodating pipe and the connecting pipe; a fixing plate is arranged at the top of the connecting column used for sealing, the fixing plate is fixedly connected with the bottom end of the interior of the connecting pipe, and through holes are correspondingly formed in the connecting column and the fixing plate in a penetrating mode; the outer wall of the top of the containing pipe used for containing is sleeved with an extension pipe, and the top of the extension pipe is sleeved with the outer wall of the connecting column. The bottom of the sleeve used for adjustment is fixedly connected with the communicating piece. The device has the advantages that separation and collection are facilitated, and height adjustment is facilitated to adapt to solutions with different heights.

Description

Technical Field

[0001] The utility model relates to the technical field of chromatography separation, and particularly relates to a chromatography separation and collection device. Background Art

[0002] When deoxynucleotides are processed, chromatography separation is a relatively common processing method, which utilizes the qualitative differences in the physical and chemical properties of the components in the sample to distribute the components in two phases to different degrees. One phase is fixed, and the other phase flows through this fixed phase (referred to as the mobile phase) and makes the components move at different speeds, so as to achieve the separation method.

[0003] However, in actual use, the liquid after chromatography separation still needs to be separated and collected. The common chromatography test tubes are usually of an integral structure, so it is not convenient to separate the device. And the lengths of the few combined test tubes that can be separated are also fixed, so it is not possible to adjust the separation well for liquids of different heights. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a chromatography separation and collection device, which solves the problems that the existing chromatography device needs to be convenient for separation and collection, and the chromatography device needs to be able to conveniently adjust the height to adapt to chromatography liquids of different heights.

[0005] In order to achieve the above purpose, the utility model provides the following technical solution: A chromatography separation and collection device, including a containing tube, a connecting tube and a connecting column. A connecting tube is arranged at the top of the containing tube, a connecting column is installed at the bottom of the connecting tube. A sleeve is vertically arranged at the center of the inner part of the connecting tube, a communicating piece is installed at the bottom of the sleeve, and extrusion rings are installed at the tops of both the containing tube and the connecting tube;

[0006] A fixing plate is arranged at the top of the connecting column for closing, and the fixing plate is fixedly connected with the inner bottom end of the connecting tube. Through holes are correspondingly arranged through the connecting column and the fixing plate;

[0007] An extension tube is sleeved on the outer wall of the top of the containing tube for containing, and the top of the extension tube is sleeved on the outer wall of the connecting column;

[0008] The bottom of the sleeve for adjusting is fixedly connected with the communicating piece, a central rod is arranged through the sleeve, an adjusting piece is arranged at the bottom of the communicating piece, and a plurality of through holes are correspondingly arranged around the inner parts of the communicating piece and the adjusting piece. The bottom end of the central rod penetrates through the inner part of the communicating piece and is fixedly connected with the adjusting piece.

[0009] Preferably, threaded grooves are arranged on one sides of the inner parts of the extrusion rings close to the connecting tube and the extension tube, and the inner walls of the sides of the connecting tube and the extension tube away from each other are inclined outwards, and sealing rings are arranged on the inclined surfaces of the inner walls of the connecting tube and the extension tube.

[0010] Preferably, the extrusion ring is threadedly connected to the outer walls of the connecting pipe and the extension pipe respectively, the sealing ring fits and presses against the inner wall of the thread groove of the extrusion ring, the size of the connecting pipe is the same as that of the extension pipe, and the diameter size of the adjustment pipe is the same as that of the receiving pipe.

[0011] Preferably, the bottom of the connecting column is fixedly connected to the top of the extension pipe, the top of the connecting column is sleeved on the inner bottom end of the connecting pipe, and a knob is installed around the center of the side wall of the connecting column.

[0012] Preferably, an adjustment pipe is slidably sleeved inside the top end of the connecting pipe, and the two sealing rings are respectively attached to the outer walls of the receiving pipe and the adjustment pipe.

[0013] Preferably, a nut is threadedly connected to the outer wall of the top of the central rod, and the bottom of the nut fits and presses against the top of the sleeve.

[0014] In the above technical solution, the technical effects and advantages provided by the present utility model are as follows:

[0015] 1. The through holes inside the connecting column are offset from the through holes inside the fixing plate, thereby blocking the liquid in the top connecting pipe from flowing downward, thereby pulling the bottom extension pipe away from the connecting column to achieve the separation of the chromatography solution. And when the liquid at the top of the connecting pipe needs to be separated again, it can push the sleeve to drive the communication piece and the adjustment piece to move to reach the stratification position, and then make the central rod rotate relative to the sleeve, thereby driving the communication piece and the adjustment piece to rotate relative to each other, so as to offset the positions of the through holes inside them, and further achieve the blocking of the liquid and realize the re-separation.

[0016] 2. Rotate the extrusion ring, so that the extrusion of the sealing ring by the extrusion ring is released, thereby conveniently pulling the extension pipe, and then adjusting the height position of the connecting column to make it the same as the height of the corresponding chromatography liquid. At the same time, it can also pull the adjustment pipe to adjust the position of the adjustment pipe, which can drive the communication piece to adjust the position. At the same time, the sleeve can be moved alone to drive the communication piece to adjust the position, so as to adapt to the separation needs of chromatography liquids at different heights. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments recorded in the present utility model. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings.

[0018] Figure 1 Schematic diagram of the present utility model;

[0019] Figure 2Internal structure sectional view of the present utility model;

[0020] Figure 3 Front view of the internal structure section of the present utility model;

[0021] Figure 4 Schematic diagram of the internal sectional structure of the connecting pipe and the adjusting pipe of the present utility model;

[0022] Figure 5 External structure schematic diagram of the connecting column of the present utility model.

[0023] Explanation of reference numerals in the drawings:

[0024] 1. Receiving pipe; 101. Extension pipe; 2. Connecting pipe; 201. Adjusting pipe; 3. Connecting column; 301. Fixed plate; 302. Knob; 4. Sleeve; 401. Central rod; 402. Nut; 5. Extrusion ring; 501. Sealing ring; 6. Connecting piece; 601. Adjusting piece. Detailed implementation manners

[0025] In order to enable those skilled in the art to better understand the technical solution of the present utility model, the present utility model will be further introduced in detail below with reference to the accompanying drawings.

[0026] The present utility model provides a chromatography separation and collection device as shown in Figures 1-5 , which includes a receiving pipe 1, a connecting pipe 2 and a connecting column 3. A connecting pipe 2 is provided at the top of the receiving pipe 1, and a connecting column 3 is installed at the bottom of the connecting pipe 2. A sleeve 4 is vertically arranged at the center of the inside of the connecting pipe 2, and a connecting piece 6 is installed at the bottom of the sleeve 4. Extrusion rings 5 are installed at the tops of both the receiving pipe 1 and the connecting pipe 2; a fixed plate 301 is provided at the top of the connecting column 3 for closing, and the fixed plate 301 is fixedly connected to the inner bottom end of the connecting pipe 2. Through holes are correspondingly provided through the inside of both the connecting column 3 and the fixed plate 301; an extension pipe 101 is sleeved on the outer wall of the top of the receiving pipe 1 for receiving, and the top of the extension pipe 101 is sleeved on the outer wall of the connecting column 3; the bottom of the sleeve 4 for adjusting is fixedly connected to the connecting piece 6. A central rod 401 is arranged through the inside of the sleeve 4, an adjusting piece 601 is provided at the bottom of the connecting piece 6, a plurality of through holes are correspondingly arranged around the inside of both the connecting piece 6 and the adjusting piece 601, and the bottom end of the central rod 401 penetrates through the inside of the connecting piece 6 and is fixedly connected to the adjusting piece 601.

[0027] As shown in Figure 2 、 Figure 4As shown in the figure, threaded grooves are provided on one side inside the extrusion ring 5 near the connecting pipe 2 and the extension pipe 101. The inner walls of the sides of the connecting pipe 2 and the extension pipe 101 that are away from each other are inclined outward. Sealing rings 501 are provided on the inclined surfaces of the inner walls of the connecting pipe 2 and the extension pipe 101. The extrusion ring 5 is threadedly connected to the outer walls of the connecting pipe 2 and the extension pipe 101 respectively. The sealing ring 501 is in mutual fit and extrusion with the inner wall of the threaded groove of the extrusion ring 5. The size of the connecting pipe 2 is the same as that of the extension pipe 101. The diameter size of the adjustment pipe 201 is the same as the diameter size of the receiving pipe 1. By conveniently rotating the extrusion ring 5, the extrusion of the sealing ring 501 by the extrusion ring 5 is released, so that the sealing ring releases the extrusion of the extension pipe 101 and the adjustment pipe 201. By conveniently pulling the extension pipe 101, the height position of the connecting column 3 can be adjusted to be the same as the height of the corresponding chromatographic liquid.

[0028] As Figure 3 , Figure 5 shown in the figure, the bottom of the connecting column 3 is fixedly connected to the top of the extension pipe 101. The top of the connecting column 3 is sleeved on the inner bottom end of the connecting pipe 2. A knob 302 is installed around the center of the side wall of the connecting column 3. The adjustment pipe 201 is slidably sleeved inside the top end of the connecting pipe 2. The two sealing rings 501 are respectively in contact with the outer walls of the receiving pipe 1 and the adjustment pipe 201. By conveniently rotating the knob 302, the connecting column 3 rotates relative to the connecting pipe 2 at its top, so that the through holes inside the connecting column 3 and the through holes inside the fixing plate 301 are staggered from each other, thereby blocking the flow of the liquid in the top connecting pipe 2 to the bottom, and pulling the bottom extension pipe 101 away from the connecting column 3 to achieve the separation of the chromatographic solution.

[0029] As Figure 3 , Figure 4 shown in the figure, a nut 402 is threadedly connected to the outer wall of the top of the central rod 401, and the bottom of the nut 402 is in mutual fit and extrusion with the top of the sleeve 4, which can push the sleeve 4 to drive the communication piece 6 and the adjustment piece 601 to move to reach the stratification position. Then, the central rod 401 and the sleeve 4 are rotated relative to each other, thereby driving the communication piece 6 and the adjustment piece 601 to rotate relative to each other, realizing the staggering of the positions of the through holes inside them, and further realizing the blocking of the liquid and achieving separation again.

[0030] When in use, the corresponding solution can be conveniently poured into the interior of the device, and then chromatography can be carried out. When separation and collection are required in the chromatography state, the knob 302 can be conveniently rotated, so that the connecting column 3 rotates relative to the connecting pipe 2 at its top, and then the through hole inside the connecting column 3 and the through hole inside the fixing plate 301 are staggered from each other, thereby blocking the liquid in the top connecting pipe 2 from flowing downward, and then pulling the extension pipe 101 at the bottom to separate from the connecting column 3, realizing the separation of the chromatography solution. And when the liquid at the top of the connecting pipe 2 needs to be separated again, the sleeve 4 can be pushed to drive the communication piece 6 and the adjusting piece 601 to move, so that they reach the stratification position, and then the central rod 401 rotates relative to the sleeve 4, thereby driving the communication piece 6 to rotate relative to the adjusting piece 601, realizing the staggering of the positions of the through holes inside it, and then realizing the blocking of the liquid and realizing the separation again.

[0031] When separating solutions with different liquid levels at different heights, the extrusion ring 5 can also be conveniently rotated, so that the extrusion of the sealing ring 501 by the extrusion ring 5 is released, so that the sealing ring contacts the extrusion of the extension pipe 101 and the adjustment pipe 201, and the extension pipe 101 can be conveniently pulled, so as to adjust the height position of the connecting column 3 to be the same as the height of the corresponding chromatography liquid. At the same time, the adjustment pipe 201 can also be pulled, so that the position of the adjustment pipe 201 is adjusted, which can drive the communication piece 6 to adjust the position, and the sleeve 4 can also be moved alone to drive the communication piece 6 to adjust the position, so as to adapt to the separation needs of chromatography liquids at different heights.

[0032] Only some exemplary embodiments of the present invention have been described in the above by way of illustration. Undoubtedly, for those of ordinary skill in the art, without departing from the spirit and scope of the present invention, the described embodiments can be modified in various different ways. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A chromatographic separation and collection device, comprising a receiving tube (1), a connecting tube (2) and a connecting column (3), characterized in that: A connecting pipe (2) is provided at the top of the receiving pipe (1). A connecting column (3) is installed at the bottom of the connecting pipe (2). A sleeve (4) is vertically arranged at the center inside the connecting pipe (2). A communicating piece (6) is installed at the bottom of the sleeve (4). Pressing rings (5) are installed at the tops of both the receiving pipe (1) and the connecting pipe (2). A fixing plate (301) is provided at the top of the connecting column (3) for closing, and the fixing plate (301) is fixedly connected to the inner bottom end of the connecting pipe (2). Through holes are correspondingly provided through the connecting column (3) and the fixing plate (301). An extension pipe (101) is sleeved on the outer wall of the top of the receiving pipe (1) for receiving, and the top of the extension pipe (101) is sleeved on the outer wall of the connecting column (3). The bottom of the sleeve (4) for adjustment is fixedly connected to the communicating piece (6). A central rod (401) is arranged through the inside of the sleeve (4). An adjusting piece (601) is provided at the bottom of the communicating piece (6). A plurality of through holes are correspondingly arranged around the inside of the communicating piece (6) and the adjusting piece (601). The bottom end of the central rod (401) penetrates through the inside of the communicating piece (6) and is fixedly connected to the adjusting piece (601).

2. The chromatographic separation and collection device according to claim 1, characterized in that: Thread grooves are provided on the inner sides of the pressing rings (5) close to the connecting pipe (2) and the extension pipe (101). The inner side walls of the connecting pipe (2) and the extension pipe (101) on the side away from each other are inclined outward, and sealing rings (501) are provided on the inclined surfaces of the inner walls of the connecting pipe (2) and the extension pipe (101).

3. The chromatographic separation and collection device according to claim 2, characterized in that: The pressing rings (5) are threadedly connected to the outer walls of the connecting pipe (2) and the extension pipe (101) respectively. The sealing rings (501) are in mutual fitting and pressing with the inner walls of the thread grooves of the pressing rings (5). The size of the connecting pipe (2) is the same as that of the extension pipe (101). The diameter size of the adjusting pipe (201) is the same as that of the receiving pipe (1).

4. The chromatographic separation and collection device according to claim 1, wherein: The bottom of the connecting column (3) is fixedly connected to the top of the extension pipe (101). The top of the connecting column (3) is sleeved on the inner bottom end of the connecting pipe (2). A knob (302) is installed around the center of the side wall of the connecting column (3).

5. The chromatographic separation and collection device according to claim 2, characterized in that: An adjusting pipe (201) is slidably sleeved inside the top end of the connecting pipe (2). The two sealing rings (501) are respectively in contact with the outer walls of the receiving pipe (1) and the adjusting pipe (201).

6. The chromatographic separation and collection device according to claim 5, wherein: A nut (402) is threadedly connected to the outer wall of the top of the central rod (401), and the bottom of the nut (402) is in mutual fitting and pressing with the top of the sleeve (4).

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