Sealed reaction device

Abstract

The utility model provides a sealed reaction device which comprises a reaction container, a sealing structure and a plug, an opening is formed in the top of the reaction container, a circulation hole is formed in the bottom of the reaction container, and the reaction container is of a soft plastic structure; an opening is formed in the reaction container, the sealing structure is plugged in the opening and detachably connected with the reaction container, the plug is plugged in the circulation hole, and the plug and the reaction container are an integrated injection molding structural part. According to the sealed reaction device provided by the invention, the probability that a sample, a reagent and the like are in contact with the external environment in the reaction process can be reduced, the risk that the sample and the reagent are polluted by aerosol is reduced, and the credibility of a reaction result is improved.

Description

Technical Field

[0001] The present disclosure relates to the technical field of biochemical reactions, and particularly to a sealed reaction device. Background Art

[0002] In the biochemical field, it is often encountered that some consecutive reactions need to be carried out at room temperature and in a closed environment. For example, nucleic acid amplification reactions are achieved through isothermal amplification technology within a short time.

[0003] In related technologies, in order to enable reactions to be carried out in a closed environment without being affected by the external environment, a sealed reaction device is usually used to achieve multiple consecutive reactions. The sealed reaction device includes a test tube with a sealed cap. Before the reaction, reaction reagents can be pre-loaded into the test tube, and then the sample is placed in the test tube for reaction. After the reaction is completed, the sealed cap is opened, and the reaction product in the test tube is transferred from the top opening to the next reaction system or directly used for detection.

[0004] However, after the reaction is completed, when transferring the reaction product in the test tube to the next reaction system or directly using it for detection, when the sealed cap is opened, the reaction product is at risk of being exposed to aerosols, affecting the reaction results and making the reaction results untrustworthy. Summary of the Utility Model

[0005] Embodiments of the present disclosure provide a sealed reaction device, which can reduce the probability of samples and reagents coming into contact with the external environment during the reaction process, reduce the risk of samples and reagents being contaminated by aerosols, and improve the credibility of reaction results. The technical solution is as follows:

[0006] Embodiments of the present disclosure provide a sealed reaction device, which includes a reaction container, a sealing structure, and a plug. The top of the reaction container has an opening, the bottom of the reaction container has a through hole, and the reaction container is of a soft plastic structure; the sealing structure plugs the opening, and the sealing structure is detachably connected to the reaction container. The plug plugs the through hole and is an integrally injection-molded structural part with the reaction container.

[0007] In another implementation manner of the present disclosure, the reaction container includes a tube body and a connecting tube. The bottom of the tube body is connected to one end of the connecting tube, and the other end of the connecting tube forms the through hole; the inner diameter of the tube body is larger than the inner diameter of the connecting tube.

[0008] In another implementation manner of the present disclosure, the inner diameter of the connecting tube is not more than 2 mm.

[0009] In another implementation manner of the present disclosure, the inner diameter of the connecting tube is not more than 1 / 5 of the inner diameter of the tube body.

[0010] In another implementation of the present disclosure, the sealed reaction device includes one of the reaction vessels, the sealing structure is plugged in the opening of the reaction vessel, the plug is plugged in the through hole, and is an integrally injection-molded structural member with the reaction vessel; or, the sealed reaction device includes a plurality of the reaction vessels, the plurality of reaction vessels are arranged in sequence along the axis of the reaction vessel, and between two adjacent reaction vessels, the tube body of one of the reaction vessels is hermetically inserted into the opening of another reaction vessel, the sealing structure is plugged in the opening of the reaction vessel at the topmost part, and the plug is plugged in the through hole of the reaction vessel at the bottommost part.

[0011] In another implementation of the present disclosure, the middle part of the tube body has an external thread, and the top of the tube body has an internal thread matching the external thread in the middle part of the tube body.

[0012] In another implementation of the present disclosure, the sealing structure has an internal thread, and the top of the tube body has an external thread matching the internal thread of the sealing structure.

[0013] In another implementation of the present disclosure, the plug is a sheet-like structure.

[0014] In another implementation of the present disclosure, the sealed reaction device further includes a sealing paper, and the sealing paper is clamped between the sealing structure and the connected reaction vessel.

[0015] In another implementation of the present disclosure, the sealing paper is one of a tin foil paper and a plastic film.

[0016] The beneficial effects brought by the technical solution provided by the embodiments of the present disclosure are as follows:

[0017] When the sealed reaction device provided by the embodiments of the present disclosure conducts a sealed reaction under normal temperature conditions, since the sealed reaction device includes a reaction vessel and the top of the reaction vessel has an opening, reaction reagents required for the reaction can be pre-added into the reaction vessel through the opening.

[0018] Also, because the sealing structure is plugged in the opening, the bottom of the reaction vessel has a through hole, and the plug is plugged in the through hole, when the reaction is carried out in the reaction vessel, the reaction vessel can be sealed simultaneously by the sealing structure and the plug, so that the reaction carried out in the reaction vessel can be in a sealed environment and is not affected by the external environment.

[0019] Also, since the reaction vessel is made of soft plastic and the plug is an integrally injection-molded structural part with the reaction vessel, after the reaction in the reaction vessel is completed and the reaction product needs to be transferred out, there is no need to open the sealing structure. Instead, the plug can be directly separated from the reaction vessel (for example, in actual operation, the plug can be cut off with scissors or directly disconnected manually), and the reaction product in the reaction vessel can flow out through the through-hole. Moreover, since the reaction vessel is made of soft plastic, the reaction vessel can be directly manually squeezed to quickly transfer the reaction product in the reaction vessel to another closed reaction system or directly used for detection without being affected by the external environment, thereby reducing the risk of sample and reagent being contaminated by aerosol and greatly improving the detection accuracy and the credibility of the reaction. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present disclosure, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present disclosure. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0021] Figure 1 Structural schematic diagram of the first sealed reaction device provided by the embodiment of the present disclosure;

[0022] Figure 2 Structural schematic diagram of the second sealed reaction device provided by the embodiment of the present disclosure;

[0023] Figure 3 Assembly diagram of a sealed reaction device provided by the embodiment of the present disclosure;

[0024] Figure 4 Assembly diagram of another sealed reaction device provided by the embodiment of the present disclosure.

[0025] The meanings of the symbols in the drawings are as follows:

[0026] 1. Reaction vessel; 101. Opening; 102. Through-hole; 11. Tube body; 12. Connecting tube;

[0027] 2. Sealing structure;

[0028] 3. Plug;

[0029] 5. Sealing paper. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] To make the objectives, technical solutions, and advantages of the present disclosure clearer, the following will further describe the embodiments of the present disclosure in detail with reference to the drawings.

[0031] The embodiment of the present disclosure provides a sealed reaction device, asFigure 1 As shown in the figure, the sealed reaction device includes a reaction vessel 1, a sealing structure 2, and a plug 3. The top of the reaction vessel 1 has an opening 101, the bottom of the reaction vessel 1 has a circulation hole 102, and the reaction vessel 1 is made of a soft plastic structure. The sealing structure 2 plugs the opening 101, and the sealing structure 2 is detachably connected to the reaction vessel 1. The plug 3 plugs the circulation hole 102 and is an integrally injection-molded structural part with the reaction vessel 1.

[0032] When the sealed reaction device provided by the embodiments of the present disclosure performs a sealed reaction under normal temperature conditions, since the sealed reaction device includes a reaction vessel 1 and the top of the reaction vessel 1 has an opening 101, reaction reagents required for the reaction can be pre-added into the reaction vessel 1 through the opening 101 in this way.

[0033] Also, because the sealing structure 2 plugs the opening 101, the bottom of the reaction vessel 1 has a circulation hole 102, and the plug 3 plugs the circulation hole 102, when the reaction is carried out in the reaction vessel 1, the reaction vessel 1 can be sealed by the sealing structure 2 and the plug 3 at the same time, so that the reaction carried out in the reaction vessel 1 can be in a closed environment without being affected by the external environment.

[0034] Also, because the reaction vessel 1 is made of a soft plastic structure and the plug 3 is an integrally injection-molded structural part with the reaction vessel 1, after the reaction in the reaction vessel 1 is completed and the reaction product needs to be transferred out, there is no need to open the sealing structure 2. Instead, the plug 3 is directly separated from the reaction vessel 1 (for example, in actual operation, the plug 3 can be cut off with scissors or directly manually disconnected), and the reaction product of the reaction vessel 1 can flow out through the circulation hole 102. Moreover, because the reaction vessel 1 is made of a soft plastic structure, the reaction vessel 1 can be directly manually squeezed, so that the reaction product in the reaction vessel 1 can be quickly transferred to another closed reaction system or directly used for detection without being affected by the external environment, thereby reducing the risk of aerosol contamination of the sample and reagent and greatly improving the detection accuracy and the credibility of the reaction.

[0035] Optionally, the reaction vessel 1 includes a tube body 11 and a connecting tube 12. The bottom of the tube body 11 is connected to one end of the connecting tube 12, and the other end of the connecting tube 12 forms the circulation hole 102. The inner diameter of the tube body 11 is larger than the inner diameter of the first connecting tube 12.

[0036] In the above implementation, the tube body 11 is used to assemble the reaction reagent and provide space for the reaction. The connecting tube 12 is used to reduce the inner diameter of the tube body 11, so that the solution in the tube body 11 will not leak out from the circulation hole 102 of the reaction vessel 1 in a static state. Instead, under the action of air pressure, a sealed water film is formed at the circulation hole 102 to plug the circulation hole 102, so that after the plug 3 leaves the tube body 11, the tube body 11 is still in a sealed state in a static state.

[0037] In other examples, the reaction vessel 1 can also have other structures. For example, it can have only a tube body without including a connecting pipe. In this case, the plug is a part of the bottom of the tube body. When it is necessary to transfer the reaction product in the tube body, one or more flow holes can be directly punched in the tube body 11.

[0038] Optionally, the inner diameter of the connecting pipe 12 is not greater than 2 mm, and the inner diameter of the connecting pipe 12 is not greater than 1 / 5 of the inner diameter of the tube body 11.

[0039] This can further limit the pipe diameter of the connecting pipe 12, enabling the connecting pipe 12 to play a throttling role and ensuring that the liquid in the connecting pipe 12 will not leak out in a static state.

[0040] In this embodiment, the inner diameter of the tube body 11 is 8 mm, and the inner diameter of the connecting pipe 12 is 1.5 mm.

[0041] Figure 2 This is a schematic structural diagram of the second sealed reaction device provided by the embodiments of the present disclosure. Refer to Figure 2 , optionally, the sealed reaction device can include one reaction vessel 1, or can also include multiple reaction vessels 1.

[0042] The sealed reaction device includes one reaction vessel 1. The sealing structure 2 is plugged in the opening 101 of the reaction vessel 1. The plug 3 is plugged in the flow hole 102 and is an integrally injection-molded structural member with the reaction vessel 1.

[0043] Or;

[0044] The sealed reaction device includes multiple reaction vessels 1. The multiple reaction vessels 1 are arranged in sequence along the axis of the reaction vessel 1. Between two adjacent reaction vessels 1, the tube body 11 of one reaction vessel 1 is hermetically plugged into the opening 101 of another reaction vessel 1. The sealing structure 2 is plugged in the opening 101 of the reaction vessel 1 at the topmost part, and the plug 3 is plugged in the flow hole 102 of the reaction vessel 1 at the bottommost part.

[0045] That is to say, when there is only one reaction vessel 1, the sealing structure 2 directly covers the top of the reaction vessel 1. When multiple reaction vessels 1 are sleeved together to achieve continuous reaction, the sealing structure 2 directly covers the opening 101 of the reaction vessel 1 at the topmost part, and between two adjacent reaction vessels 1, one reaction vessel 1 covers the opening 101 of another reaction vessel 1.

[0046] The number of reaction vessels 1 can be reasonably assembled according to the actual situation.

[0047] Optionally, the middle part of the tube body 11 has an external thread, and the top of the tube body 11 has an internal thread matching the external thread in the middle part of the tube body 11.

[0048] In the above implementation, an external thread is provided in the middle of the pipe body 11, and an internal thread is provided at the top of the pipe body 11, so that two adjacent pipe bodies 11 can be detachably connected by the thread, which is convenient for assembly.

[0049] In other examples, two adjacent pipe bodies 11 can also be connected by other means, such as, sealing tape and so on.

[0050] Optionally, the sealing structure 2 has an internal thread, and the top of the pipe body 11 has an external thread matching the internal thread of the sealing structure 2.

[0051] In the above implementation, by providing an internal thread in the sealing structure 2 and an external thread in the outer wall of the top of the pipe body 11, the sealing structure 2 can be detachably capped in the top opening 101 of the connected pipe body 11 through the thread.

[0052] In other examples, the sealing structure 2 can also be hermetically clamped to the top of the pipe body 11 through a limiting flange or the like.

[0053] In this embodiment, the sealing structure is a structural member in the form of a cap.

[0054] Optionally, the plug 3 is a sheet-like structure. The plug 3 is used to seal the connecting pipe 12 so that the bottom of the pipe body 11 can be in a sealed state.

[0055] In this embodiment, for the convenience of use, a reaction vessel 1, a sealing structure 2 and a plug 3 can be set as a product structural member.

[0056] When there is 1 reaction vessel 1, correspondingly, the product structural member is one.

[0057] When there are N reaction vessels 1 (N is greater than 1 and is a positive integer), the corresponding number of product structural members is N. If it is necessary to assemble N product structural members together to form a sealed reaction device including N reaction vessels 1, the sealing structure 2 in each product structural member can be disassembled from the reaction vessel 1 by the thread first. Then, the plug 3 in N - 1 of the product structural members is separated from the connected reaction vessel 1, and these N - 1 product structural members are sequentially sleeved in another product structural member in which the plug 3 is not separated from the reaction vessel 1. Then, one of the sealing structures 2 is hermetically capped in the opening 101 of the product structural member at the top among the assembled multiple product structural members.

[0058] Figure 3 It is an assembly drawing of a sealed reaction device provided by an embodiment of the present disclosure. Refer to Figure 3, for example, when N = 2, the sealing structure 2 in each product structural member can be disassembled and separated from the reaction vessel 1 connected by threads, and the plug 3 in one of the product structural members can be separated from the connected reaction vessel 1. Then, the reaction vessel 1 separated from the plug 3 is screwed into another reaction vessel 1, and the sealing structure 2 is screwed onto the reaction vessel 1 at the top.

[0059] Figure 4 The assembly drawing of another sealing reaction device provided by the embodiment of the present disclosure is shown in Figure 4 , when N = 3, the sealing structure 2 in each product structural member can be disassembled and separated from the reaction vessel 1 connected by threads, and the plugs 3 in two of the product structural members can be separated from the connected reaction vessels 1. Then, the two reaction vessels 1 separated from the plugs 3 are sequentially sleeved by threads and then screwed into another reaction vessel 1, and the sealing structure 2 is capped on the reaction vessel 1 at the top. That is to say, in use, reasonable assembly of different numbers of product structural members can be carried out according to actual situations. Details are not listed one by one here.

[0060] In this embodiment, the width of the plug 3 is greater than the outer diameter of the first communication pipe 12. In this way, the first communication pipe 12 can be sealed by the relatively wide plug 3, and at the same time, it is also convenient to remove the plug 3 from the first communication pipe 12.

[0061] Optionally, the sealing reaction device further includes a sealing paper 5, and the sealing paper 5 is clamped between the sealing structure 2 and the connected reaction vessel 1.

[0062] In the above implementation manner, the sealing paper 5 can further seal the sealing performance of the reaction device.

[0063] Exemplarily, one of the sealing paper 5 and the plastic film.

[0064] In this embodiment, the sealing paper 5 is a tin foil paper. The tin foil paper is convenient to arrange, has a low cost, and can also insulate heat.

[0065] The working process of the sealing reaction device provided by the embodiment of the present disclosure is briefly introduced below:

[0066] If one-step reaction operation is required for the sealing reaction device provided by the embodiment of the present disclosure, in a sterile environment, reaction reagents are pre-loaded into the reaction vessel 1 in advance, and then a sample is added, and the sealing structure 2 is tightened. After the reaction is completed, the plug 3 is cut off, and the reaction product in the reaction vessel 1 is extruded and discharged for detection.

[0067] If it is necessary to perform two-step, three-step or more consecutive reaction operations using the sealed reaction device provided by the embodiments of the present disclosure, a plurality of reaction vessels 1 are assembled together. In a sterile environment, reaction reagents are pre-loaded into each reaction vessel 1 in advance. Then, the stoppers 3 of N-1 reaction vessels are correspondingly cut off, and the reaction vessels 1 with the stoppers 3 cut off are arranged in sequence. After adjacent two reaction vessels 1 are hermetically plugged together, they are integrally screwed into the reaction vessel 1 without the stopper 3 cut off.

[0068] A sample is added to the topmost reaction vessel 1, and then the sealing structure 2 is screwed tightly to the top of the topmost reaction vessel 1. The reaction starts in the topmost reaction vessel 1. After the reaction in the topmost reaction vessel 1 ends, the reaction product in the topmost reaction vessel 1 is manually squeezed into the adjacent reaction vessel 1 for subsequent reactions. And so on until all reactions are completed.

[0069] After the reaction ends, the stopper 3 of the lowermost reaction vessel 1 is cut off, and the reagent in the lowermost reaction vessel 1 will be discharged from the communicating pipe 12. It can be seen that each of the above reaction steps and the product transfer between steps can be ensured to be carried out in a closed environment.

[0070] In the embodiments of the present disclosure, the reaction vessel 1 is provided with a double-thread system, so that the reaction vessel 1 can be self-sealed through the sealing structure 2 and can also be combined with other adjacent reaction vessels 1 into a closed structure. The bottom of the reaction vessel 1 is provided with a stopper 3. After cutting off the stopper 3, the reagent in the reaction vessel 1 can still be kept inside the reaction vessel 1 without extrusion, and it is very convenient to discharge the reaction product after extrusion.

[0071] The above are only optional embodiments of the present disclosure and are not intended to limit the present disclosure. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present disclosure shall be included within the protection scope of the present disclosure.

Claims

1. A sealed reaction device, characterized in that, The sealed reaction device includes a reaction vessel (1), a sealing structure (2), and a plug (3); The top of the reaction vessel (1) has an opening (101), the bottom of the reaction vessel (1) has a circulation hole (102), and the reaction vessel (1) is made of a soft plastic structure; The sealing structure (2) is plugged in the opening (101), and the sealing structure (2) is detachably connected to the reaction vessel (1). The plug (3) is plugged in the circulation hole (102) and is an integrally injection-molded structural part with the reaction vessel (1).

2. The sealed reaction device according to claim 1, characterized in that, The reaction vessel (1) includes a tube body (11) and a connecting tube (12). The bottom of the tube body (11) is connected to one end of the connecting tube (12), and the other end of the connecting tube (12) forms the circulation hole (102); The inner diameter of the tube body (11) is larger than the inner diameter of the connecting tube (12).

3. The sealed reaction device according to claim 2, wherein The inner diameter of the connecting tube (12) is not greater than 2 mm.

4. The sealed reaction device according to claim 3, characterized in that, The inner diameter of the connecting tube (12) is not greater than 1 / 5 of the inner diameter of the tube body (11).

5. The sealed reaction device according to claim 4, wherein The sealed reaction device includes one reaction vessel (1). The sealing structure (2) is plugged in the opening (101) of the reaction vessel (1), and the plug (3) is plugged in the circulation hole (102) and is an integrally injection-molded structural part with the reaction vessel (1); Or, The sealed reaction device includes a plurality of reaction vessels (1). The plurality of reaction vessels (1) are arranged in sequence along the axis of the reaction vessel (1). Between two adjacent reaction vessels (x), the tube body (11) of one reaction vessel (1) is hermetically inserted into the opening (101) of the other reaction vessel (1). The sealing structure (2) is plugged in the opening (101) of the topmost reaction vessel (1), and the plug (3) is plugged in the circulation hole (102) of the bottommost reaction vessel (1).

6. The sealed reaction device according to claim 5, wherein The middle part of the tube body (11) has an external thread, and the top of the tube body (11) has an internal thread matching the external thread in the middle part of the tube body (11).

7. The sealed reaction device according to claim 6, wherein, The sealing structure (2) has an internal thread, and the top of the tube body (11) has an external thread matching the internal thread of the sealing structure (2).

8. The sealed reaction device according to any one of claims 1-7, characterized in that, The plug (3) is a sheet-like structure.

9. The sealed reaction device according to any one of claims 1-7, characterized in that, The sealed reaction device further includes a sealing paper (5). The sealing paper (5) is clamped between the sealing structure (2) and the connected reaction vessel (1).

10. The sealed reaction device according to claim 9, characterized in that, The sealing paper (5) is one of a tin foil paper and a plastic film.

Images