PCR (Polymerase Chain Reaction) row tube cover and combined PCR reaction consumable

Abstract

The utility model belongs to the field of biological consumables, and particularly relates to a PCR row pipe cover and a combined PCR reaction consumable, the PCR row pipe cover comprises a cover body, a plurality of plug connectors and a plurality of soft rubber caps, the number of the soft rubber caps is the same as that of the plug connectors, and the plug connectors are arranged on the same side of the cover body and correspond to openings of reaction pipes in a PCR plate in a one-to-one mode. The plurality of soft rubber caps sleeve the plurality of plug connectors in a one-to-one correspondence manner, and the outer sides of the soft rubber caps are provided with annular protrusions arranged in the circumferential direction. The plug connectors on the cover body are sleeved with the soft rubber caps made of soft materials, the outer sides of the soft rubber caps are provided with the annular protrusions arranged in the circumferential direction, the annular protrusions are equivalent to O-shaped rings, and when the cover is sealed, the plug connectors on one side of the cover body are pressed into the openings of the reaction tubes in the PCR plate in a one-to-one correspondence mode; openings of the reaction tubes in the PCR plate are sealed in the mode that the soft rubber caps are matched with the O-shaped rings, capping is convenient, sealing is reliable, the sealing performance of the capping is guaranteed, and then the risk that PCR reactions interfere with one another in the detection process is completely eradicated.

Description

Technical Field

[0001] The present application relates to the technical field of biological consumables, and in particular to a PCR strip tube cover and a combined PCR reaction consumable. Background Art

[0002] Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be seen as a special form of DNA replication outside of an organism. The most significant feature of PCR is its ability to significantly increase trace amounts of DNA. PCR reactions require the use of PCR tubes.

[0003] Existing technologies generally use single PCR reaction tubes and single-row PCR reaction tubes, or ordinary single-color flat lids. Multi-row PCR reaction tubes are inconvenient to cap, which will affect the smoothness of the test and increase the reaction time. The sealing of the cap cannot be guaranteed, and there is a risk of mutual interference during the test. Utility Model Content

[0004] The embodiments of the present application provide a PCR tube cap and a modular PCR reaction consumable, which are used to solve the technical problems of inconvenient capping of multiple PCR tubes and poor sealing of the caps.

[0005] To this end, according to one aspect of the present application, a PCR strip tube cover is provided, which is used to be installed on the top of a PCR plate and seal the openings of the reaction tubes in the PCR plate. The PCR strip tube cover includes:

[0006] Cover body;

[0007] A plurality of connectors are provided on the same side of the cover and correspond one-to-one to the openings of the reaction tubes in the PCR plate; and

[0008] A plurality of soft rubber caps are sleeved on the plurality of connectors in a one-to-one correspondence, and the outer sides of the soft rubber caps are provided with an annular protrusion arranged along the circumferential direction.

[0009] Optionally, at least two annular protrusions are provided on the outer side of the soft rubber cap at intervals in the plugging and unplugging direction of the connector.

[0010] Optionally, a gripping portion for gripping by a robot arm or a robot head is provided on a side of the cover body facing away from the connector.

[0011] Optionally, the connector is a hollow cylinder, and a through hole communicating with the interior of each hollow cylinder in a one-to-one correspondence is formed on a side of the cover body away from the connector, and the through hole forms the grasping portion.

[0012] Optionally, a side of the cover body facing away from the connectors is provided with different numbers corresponding to the connectors.

[0013] Optionally, the cover body and the plurality of plug connectors are of an integral structure.

[0014] Optionally, the plug connectors are made of hard plastic, and / or the material of the soft rubber cap is TPA.

[0015] According to another aspect of the present application, there is provided a combined PCR reaction consumable, comprising:

[0016] a PCR plate; and

[0017] the PCR multi-well tube cap as described above, which is used to be installed on the top of the PCR plate, and seal the openings of the reaction tubes in the PCR plate through the plug connectors and the soft rubber caps.

[0018] Optionally, the PCR plate includes a panel and a plurality of reaction tubes arranged below the panel and connected to the panel. Avoidance holes corresponding to the tube openings of the reaction tubes are provided on the panel. A circular convex edge is provided around the avoidance holes on the panel. A protrusion for abutting against the panel is provided on the side of the cover body where the plug connectors are provided. The height of the protrusion is equal to the height of the convex edge.

[0019] Optionally, the shape of the cover body is adapted to the shape of the panel.

[0020] The beneficial effects of the PCR multi-well tube cap and the combined PCR reaction consumable provided by the present application are as follows: Compared with the prior art, the PCR multi-well tube cap of the present application is sleeved with a soft rubber cap on the plug connectors on the cover body. The outer side of the soft rubber cap has a circumferentially arranged annular protrusion, and the annular protrusion is equivalent to an O-ring. When sealing, the plug connectors on one side of the cover body are respectively pressed into the openings of the reaction tubes in the PCR plate, and the openings of the reaction tubes in the PCR plate are sealed in the form of the soft rubber cap cooperating with the O-ring. The capping is convenient and the sealing is reliable, ensuring the sealing performance of the capping, and further eliminating the risk of mutual interference in the PCR reaction during detection. Description of the Drawings

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required to be used in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0022] Among them:

[0023] Figure 1 is a schematic perspective view of a PCR multi-well tube cap shown in an embodiment of the present application;

[0024] Figure 2 is Figure 1 A schematic perspective view of another angle of the PCR multi-well tube cap shown;

[0025] Figure 3 A schematic cross-sectional view of the PCR multi-well tube cap shown in an embodiment of the present application;

[0026] Figure 4 A schematic perspective view of a combined PCR reaction consumable shown in an embodiment of the present application;

[0027] Figure 5 A schematic cross-sectional view of a combined PCR reaction consumable shown in an embodiment of the present application;

[0028] Figure 6 A schematic perspective view of a PCR plate in a combined PCR reaction consumable shown in an embodiment of the present application.

[0029] Description of main component symbols:

[0030] 100, PCR multi-well tube cap;

[0031] 110, cap body; 111, grasping part; 112, label; 113, protrusion;

[0032] 120, plug-in part;

[0033] 130, soft rubber cap; 131, annular protrusion;

[0034] 200, PCR plate;

[0035] 210, panel; 211, avoidance hole; 212, convex edge;

[0036] 220, reaction tube. Detailed implementation manners

[0037] To facilitate the understanding of the present application, the present application will be described more comprehensively below with reference to the relevant drawings. The preferred embodiments of the present application are shown in the drawings. However, the present application can be implemented in many other different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure content of the present application more thorough and comprehensive.

[0038] It should be noted that when an element is referred to as "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0039] It should be understood that the orientation or positional relationship indicated by the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application. In the description of the present application, the meaning of "a plurality" is two or more, unless otherwise specifically defined.

[0040] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which this application belongs. The terms used in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0041] It should also be noted that in the embodiments of the present application, the same reference numerals are used to represent the same components or the same parts. For the same parts in the embodiments of the present application, only one of the parts or components may be marked with a reference numeral in the figure. It should be understood that the reference numerals are equally applicable to other identical parts or components.

[0042] According to one aspect of the present application, an embodiment of the present application provides a PCR multi-well tube cap, which is used to be installed on the top of a PCR plate and seal the openings of the reaction tubes in the PCR plate. As Figures 1 - 3 shown, the PCR multi-well tube cap 100 includes a cap body 110, a plurality of plug connectors 120, and a plurality of soft rubber caps 130 having the same number as the plug connectors 120. The plurality of plug connectors 120 are arranged on the same side of the cap body 110 and respectively correspond to the openings of the reaction tubes in the PCR plate one by one. The plurality of soft rubber caps 130 are respectively sleeved on the plurality of plug connectors 120 one by one, and the outer side of the soft rubber cap 130 has a circumferentially arranged annular protrusion 131.

[0043] In the embodiment of the present application, the PCR multi-well tube cap 100 is provided with a soft rubber cap 130 with a relatively soft material sleeved on the plug connector 120 on the cap body 110. The outer side of the soft rubber cap 130 has a circumferentially arranged annular protrusion 131. The annular protrusion 131 is equivalent to an O-ring. When sealing, as Figures 4 - 5 shown, the plug connectors 120 on one side of the cap body 110 are respectively pressed into the openings of the reaction tubes 220 in the PCR plate 200 one by one, and the openings of the reaction tubes 220 in the PCR plate 200 are sealed by using the form of the soft rubber cap 130 cooperating with the O-ring. The capping is convenient and the sealing is reliable, ensuring the sealing performance of the capping, and further eliminating the risk of mutual interference in the PCR reaction during detection.

[0044] Among them, the soft rubber cap 130 can be made of TPA (fully known as Transparent Polyamide). TPA is similar to nylon and has high strength, wear resistance, oil resistance and heat resistance, and can be used to replace silicone rubber. Of course, it can be conceived that the soft rubber cap 130 can also be made of silicone material, etc., as long as the texture is relatively soft and has good elastic deformation ability, which is not limited here.

[0045] It should be noted that the soft rubber cap 130 and the plug-in member 120 can be connected by bonding or interference fit, or can be integrally formed by two-material (the plug-in member 120 is made of hard PP material and the soft rubber cap 130 is made of soft TPA material) injection molding to form an integral structure.

[0046] In one embodiment, as Figures 1 - 3 shown, at least two annular protrusions 131 are provided at intervals on the outer side of the soft rubber cap 130 in the plugging and unplugging direction of the plug-in member 120.

[0047] The setting of multiple annular protrusions 131 is beneficial to increasing the contact area with the inner wall of the opening of the reaction tube, thereby improving the sealing ability.

[0048] Specifically, the soft rubber cap 130 and the annular protrusion 131 are of an integral structure and are integrally formed by injection molding.

[0049] In one embodiment, as Figure 1 shown, a grasping portion 111 for a manipulator or a robotic head to grasp is provided on the side of the cover body 110 facing away from the plug-in member 120.

[0050] The setting of the grasping portion 111 can be adapted to a variety of manipulators or robotic heads for use in automatic lid opening and transfer. The manipulator or robotic head cooperates with the grasping portion 111 on the cover body 110 to drive the PCR row tube lid 100 to be hermetically connected to and separated from the opening of the reaction tube in the PCR plate. When the manipulator or robotic head connects the grasping portion 111 to dock with the opening of the reaction tube on the PCR plate, it is convenient for the plug-in member 120 to be positioned and inserted.

[0051] In a specific embodiment, as Figure 3 shown, the plug-in member 120 is a hollow cylinder, and through holes corresponding to the inside of each hollow cylinder are opened on the side of the cover body 110 facing away from the plug-in member 120, and the through holes form the grasping portion 111.

[0052] In other embodiments, a block-shaped or strip-shaped structure may also be provided on the side of the cover 110 facing away from the plug-in member 120 as a grasping portion 111 for a manipulator or a robotic head to grasp. It can be understood that the function of the grasping portion 111 is to facilitate the grasping by the manipulator or the robotic head, and then to transfer and apply a force to the PCR multi-well tube cap 100, and its shape and structure can be flexibly designed.

[0053] In a specific embodiment, as Figure 1 shown, on the side of the cover 110 facing away from the plug-in member 120, different labels 112 are provided corresponding to each plug-in member 120.

[0054] Combined with Figure 4 and Figure 6 shown, the labels 112 on the cover 110 correspond one-to-one with the labels near the openings of the reaction tubes 220 on the PCR plate 200, which facilitates the corresponding insertion of the reaction tubes 220 with the same label 112 and the plug-in members 120 when sealing the cover.

[0055] Specifically, the label 112 can adopt digital labels "1, 2, 3, 4, 5, 6...", or can also adopt letter labels "A, B, C, D, E, F...".

[0056] In one embodiment, as Figures 1 - 3 shown, the cover 110 and the plurality of plug-in members 120 are of an integral structure.

[0057] With this setting, it is convenient for production and manufacturing. At the same time, it is beneficial to improve the connection strength between the plug-in member 120 and the cover 110.

[0058] Specifically, the cover 110 and the plurality of plug-in members 120 can be integrally injection-molded with hard plastic on a mold.

[0059] Among them, the hard plastic can adopt polypropylene (abbreviated as PP).

[0060] According to another aspect of the present application, an embodiment of the present application also provides a combined PCR reaction consumable, as Figures 4 - 5 shown, the combined PCR reaction consumable includes a PCR plate 200 and the PCR multi-well tube cap 100 in any of the above embodiments. The PCR multi-well tube cap 100 is used to be installed on the top of the PCR plate 200, and seals the openings of the reaction tubes 220 in the PCR plate 200 through the plug-in members 120 and the soft rubber caps 130.

[0061] It is understandable that the plug-in parts 120 in the PCR multi-well tube cap 100 and the reaction tubes 220 in the PCR plate 200 are arranged in the same array pattern. In the figure, an array pattern of 2 rows and 4 columns (2*4) is shown, and other combination patterns (such as 2*8, 4*4, 4*8, 8*8, etc.) can be extended with this as the minimum unit.

[0062] Since the PCR multi-well tube cap 100 in the above embodiment is adopted, the combined PCR reaction consumables have the advantages of being convenient for opening / covering and having reliable sealing, which is beneficial to improving the efficiency and accuracy of the PCR reaction.

[0063] In one embodiment, as Figure 6 shown, the PCR plate 200 includes a panel 210 and a plurality of reaction tubes 220 disposed below the panel 210 and connected to the panel 210. The shape of the cover body 110 is adapted to the shape of the panel 210. The panel 210 is provided with avoidance holes 211 corresponding to the tube openings of the reaction tubes 220. A circular convex edge 212 is provided around the avoidance holes 211 on the panel 210. One side of the cover body 110 provided with the plug-in part 120 is provided with a protrusion 113 for abutting against the panel 210, and the height of the protrusion 113 is equal to the height of the convex edge 212.

[0064] When the PCR multi-well tube cap 100 is covered on the PCR plate 200 in place, the convex edge 212 provided around the avoidance holes 211 on the panel 210 abuts against the cover body 110, and the protrusion 113 provided on one side of the cover body 110 provided with the plug-in part 120 abuts against the panel 210, improving the stability between the PCR multi-well tube cap 100 and the PCR plate 200.

[0065] The technical features of the above embodiments can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the above embodiments are described. However, as long as the combinations of these technical features do not conflict, they should be considered as the scope described in this specification.

[0066] The above embodiments only represent several implementation manners of the present application. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several deformations and improvements can still be made, and these all belong to the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the appended claims.

Claims

1. A PCR multi-well tube cap is used to be installed on the top of a PCR plate (200) and seal the openings of reaction tubes (220) in the PCR plate (200), and is characterized in that, The PCR strip tube cover (100) comprises: Cover (110); A plurality of connectors (120) are arranged on the same side of the cover (110) and correspond one-to-one to the openings of the reaction tubes (220) in the PCR plate (200); and A plurality of soft rubber caps (130) are sleeved on the plurality of connectors (120) in a one-to-one correspondence, and an annular protrusion (131) is provided on the outer side of the soft rubber cap (130) along the circumferential direction.

2. The PCR multi-well tube cap according to claim 1, wherein, At least two annular protrusions (131) are provided on the outer side of the soft rubber cap (130) at intervals in the plugging and unplugging direction of the connector (120).

3. The PCR multi-well tube cap according to claim 1, wherein A gripping portion (111) for gripping by a robot arm or a robot head is provided on a side of the cover body (110) facing away from the plug connector (120).

4. The PCR multi-well tube cap according to claim 3, wherein, The connector (120) is a hollow cylinder, and a through hole is provided on the side of the cover (110) facing away from the connector (120), which is connected to the interior of each hollow cylinder in a one-to-one correspondence, and the through hole forms the grasping portion (111).

5. The PCR multi-well tube cap according to claim 1, characterized in that, A side of the cover (110) facing away from the plug-in connector (120) is provided with a different label (112) corresponding to each plug-in connector (120).

6. The PCR multi-well tube cap according to claim 1, wherein The cover body (110) and the plurality of connectors (120) are an integrated structure.

7. The PCR multi-well tube cap according to claim 1, wherein The connector (120) is made of hard plastic, and / or the soft plastic cap (130) is made of TPA.

8. A combined PCR reaction consumable, characterized in that, include: PCR plate (200); as well as The PCR strip tube cover (100) according to any one of claims 1 to 7, wherein the PCR strip tube cover (100) is used to be installed on the top of the PCR plate (200), and to seal the openings of the reaction tubes (220) in the PCR plate (200) through the connector (120) and the soft rubber cap (130).

9. The combined PCR reaction consumable according to claim 8, wherein, The PCR plate (200) comprises a panel (210) and a plurality of reaction tubes (220) arranged below the panel (210) and connected to the panel (210); the panel (210) is provided with a avoidance hole (211) corresponding to the tube opening of the reaction tube (220); the panel (210) is provided with a circle of ridges (212) surrounding the avoidance hole (211); the cover (110) is provided with a protrusion (113) for supporting the panel (210) on one side where the plug-in component (120) is provided; the height of the protrusion (113) is equal to the height of the ridge (212).

10. The combined PCR reaction consumable according to claim 9, wherein, The shape of the cover (110) is adapted to the shape of the panel (210).

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