Stirring mechanism and cell culture bag
Through the stirring mechanism with simple and single snap-on connection, the loosening and complex installation problems of the stirring paddle of the cell culture bag are solved, and stable installation and simple disassembly are achieved, reducing costs.
Patent Information
- Application Number
- CN202422301730.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-20
- Publication Date
- 2025-08-01
- Estimated Expiration
- 2034-09-20
AI Technical Summary
The existing cell culture bag stir paddle fixation method has the risk of loosening or complex installation and high cost.
Using a stirring mechanism with simple single snap-on connection, the design of a stirring rod, agitator paddle and keys is used to achieve stable installation using elastic cantilever and beveled structure, simplifying operation and reducing costs.
The stirring paddle is stable and easy to disassemble, reducing operational complexity and cost.
Smart Images
Figure CN223170736U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of cell culture, and specifically relates to a stirring mechanism and a cell culture bag using the stirring mechanism. Background Art
[0002] A bioreactor refers to an appropriate environment or engineering equipment that provides a biochemical reaction. Generally, it refers to a device that uses enzymes or organisms (microorganisms) to enable the device to have a function similar to that of a living being, and can carry out biochemical reactions outside cells. Aerobic reactions and anaerobic reactions can be carried out during the simulation process. In terms of source, it can be divided into natural bioreactors and engineering bioreactors, etc. Bioreactors are usually cylindrical, with volumes ranging from several liters to several cubic meters, and are often made of stainless steel, borosilicate glass or plastic bags.
[0003] A cell culture bag generally includes a bag body. A stirring paddle is arranged inside the bag body, and an air vent plate and a liquid discharge port are arranged at the bottom of the bag body. Existing stirring paddles are usually fixed on a rotating shaft by screws or fixed on the rotating shaft by using a composite buckle structure. When a cell culture bag is used for cell culture, if the culture period is long, there is a risk that the stirring paddle fixed by screws will become loose, and there will be a relatively large dead volume in the gap between the screw and the thread. The composite buckle has a complex structure, difficult connection and installation, and cannot be disassembled after installation, which has high operation requirements and high costs. Summary of the Utility Model
[0004] In order to solve the above problems, the utility model provides a stirring mechanism, which includes a stirring rod, a stirring paddle and a key. The stirring paddle includes a shaft sleeve and a paddle blade. The paddle blade is located on the outer periphery of the shaft sleeve. A first strip-shaped groove extending along the axial direction is arranged on the inner periphery of the shaft sleeve. A second strip-shaped groove is arranged on the stirring rod. The key is strip-shaped, and the length of the key is the same as the length of the second strip-shaped groove.
[0005] The key has a first surface and a second surface arranged oppositely. Protrusions are respectively arranged at the upper and lower ends of the first surface, so as to form a concave surface in the middle of the first surface. The length of the concave surface is the same as the length of the shaft sleeve. After the key is placed in the second strip-shaped groove, the concave surface protrudes from the outer periphery of the stirring rod, so as to be combined with the first strip-shaped groove.
[0006] Further, the second surface is stepped, so that the key forms an elastic cantilever with a smaller thickness above relative to below, or the key forms an elastic cantilever with a smaller thickness below relative to above.
[0007] Further, on the first surface, the protrusions located on the elastic cantilever have inclined surfaces that are inclined from the end of the key towards the middle and outside of the key.
[0008] The present utility model also provides a cell culture bag, which includes a bag body, and the above-mentioned stirring mechanism is arranged inside the bag body.
[0009] Furthermore, a driving device interface is also arranged on the cell culture bag. The driving device interface is used to externally connect a driving device, and the external driving device drives the stirring mechanism to rotate.
[0010] In the present utility model, the paddle of the stirring mechanism is installed by a simple single snap-fastener connection method, which is simple and reliable, and the installation operation is simple. Moreover, the single snap-fastener is a low-value-added consumable part. When disassembling, damaging the single snap-fastener can ensure the integrity and detachability of the stirring paddle. Description of the Drawings
[0011] Figure 1 is the structural diagram of the cell culture bag;
[0012] Figure 2 is the disassembled diagram of the main components of the stirring mechanism;
[0013] Figure 3 is the installation schematic diagram of the stirring mechanism. Detailed Embodiment
[0014] See Figure 1 , which is the structural schematic diagram of the cell culture bag applying the stirring mechanism. The cell culture bag includes a bag body 1, an air vent disk 2, a stirring mechanism 3, a plurality of pipeline interfaces 4, and a driving device interface 5. The stirring mechanism 3 is located inside the bag body 1, and the plurality of pipeline interfaces 4 are used for the liquid communication between the inside and the outside of the culture bag. The driving device interface 5 is used to externally connect a driving device to drive the stirring mechanism 3 to rotate.
[0015] See Figure 2 , the stirring mechanism 3 includes a stirring rod 31, a stirring paddle 32, and a key 33. The stirring paddle 32 includes a bushing 321 and a blade 322. The blade 322 is located on the outer periphery of the bushing 321, and a first elongated groove 3211 extending along the axial direction is arranged on the inner periphery of the bushing 321.
[0016] A second elongated groove 311 is arranged on the stirring rod 31. The key 33 is elongated, and the length of the key 33 is the same as the length of the second elongated groove 311. The width of the key 33, the width of the first elongated groove 3211, and the width of the second elongated groove 311 are the same;
[0017] The key 33 has a first surface 331 and a second surface 332 which are oppositely arranged. Protrusions 333 are respectively arranged at the upper and lower ends of the first surface 331, so as to form a concave surface 334 in the middle of the first surface 331. The length of the concave surface 334 is the same as the length of the bushing 321. After the key 33 is placed in the second elongated groove 311, the concave surface 334 protrudes from the outer periphery of the stirring rod 31 to facilitate the combination with the first elongated groove 3211.
[0018] The second surface 332 is stepped, so that the key 33 forms a resilient cantilever with a smaller thickness above or below (in Figure 2 , the cantilever is located above). The step can be a step with a vertical surface transition, or it can be Figure 2 the step with an inclined surface transition as shown. Different transition forms result in different compressive effects of the resilient cantilever. On the first surface 331, the protrusion 333 located on the cantilever has an inclined surface that slopes from the end of the key 33 towards the middle of the key and towards the outside (the first surface 331 is the outside and the second surface 332 is the inside).
[0019] See Figure 3 , which is a schematic diagram after the installation of the stirring mechanism. The process of installing the stirring paddle 32 is as follows: Place the key 33 in the second strip-shaped groove 311, and then slip the bushing 321 onto one side of the stirring rod 31. In the figure, since the resilient cantilever is located in the upper part, the bushing 321 is slipped onto the stirring rod from the upper part downwards, and the first strip-shaped groove 3211 is also aligned with the key 33. When the bushing 321 contacts the key 33, due to the guidance of the inclined surface of the upper protrusion 333, the resilient cantilever is squeezed and elastically deformed into the second strip-shaped groove 311, and the bushing 321 can continue to move downwards. When the lower end of the bushing 321 abuts against the protrusion at the lower end of the key 33, the resilient cantilever rebounds, thus firmly fixing the bushing 321 in the concave surface 334 and preventing it from moving up and down. At the same time, since a part of the key 33 is received in the first strip-shaped groove 3211, the bushing 321 cannot rotate relative to the stirring rod 31 either.
[0020] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A stirring mechanism, characterized in that, It includes a stirring rod (31), a stirring paddle (32) and a key (33). The stirring paddle (32) includes a bushing (321) and a blade (322). The blade (322) is located on the outer periphery of the bushing (321). A first rectangular groove (3211) extending along the axial direction is provided on the inner periphery of the bushing (321). A second rectangular groove (311) is provided on the stirring rod (31). The key (33) is strip-shaped, and the length of the key (33) is the same as that of the second rectangular groove (311). The key (33) has a first surface (331) and a second surface (332) which are oppositely arranged. Protrusions (333) are respectively provided at the upper and lower ends of the first surface (331), so as to form a concave surface (334) in the middle of the first surface (331). The length of the concave surface (334) is the same as that of the bushing (321). After the key (33) is placed in the second rectangular groove (311), the concave surface (334) protrudes from the outer periphery of the stirring rod (31) to facilitate combination with the first rectangular groove (3211).
2. The stirring mechanism according to claim 1, characterized in that, The second surface (332) is stepped, so that the key (33) forms an elastic cantilever with a smaller thickness above relative to below, or the key (33) forms an elastic cantilever with a smaller thickness below relative to above.
3. The stirring mechanism according to claim 2, wherein On the first surface (331), the protrusions (333) located on the elastic cantilever have inclined surfaces that incline from the end of the key (33) towards the middle and the outside of the key.
4. A cell culture bag, comprising a bag body, characterized in that, The bag body is provided with the stirring mechanism according to any one of claims 1-3.
5. The cell culture bag according to claim 4, characterized in that, The cell culture bag is further provided with a driving device interface (5). The driving device interface (5) is used to externally connect a driving device, and the externally connected driving device drives the stirring mechanism to rotate.