Nucleic acid production ammonolysis device convenient to operate

Abstract

The utility model discloses a nucleic acid production ammonolysis device convenient to operate, which belongs to the technical field of ammonolysis devices and comprises a mounting shell component, a mounting limiting component used for fixing a synthetic column is mounted in the mounting shell component, and a rotating motor used for rotating the mounting limiting component is mounted on the mounting shell component. And a quantitative injection assembly for quantitatively injecting ammonolysis liquid and acetonitrile is mounted on the mounting shell assembly. By adopting the mode, the quantitative injection of ammonolysis liquid and acetonitrile is realized, the most efficient and accurate reagent amount in the reaction is ensured, the reaction is always under the optimal reaction condition, meanwhile, the accurate quantitative injection can reduce the waste of the reagent and lower the production cost, and the synthetic columns can be placed and taken in batches, so that the production efficiency is improved. The operation is more convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of ammonolysis devices, and particularly relates to an ammonolysis device for nucleic acid production that is convenient to operate. Background Art

[0002] The solid-phase synthesis method is an efficient method for synthesizing oligonucleic acids. First, a functional group (such as a phosphate group) of a nucleotide is bound to a solid-phase support to form a stable solid-phase matrix. After the synthesis is completed, the product needs to be released from the solid-phase support. The ammonolysis reaction is a key step to achieve this process. Usually, ammonia water or other ammonia sources are added to hydrolyze the linking groups of the nucleic acid chain, so as to cleave the oligonucleic acid product from the solid-phase support. In the purification step of oligonucleic acids, acetonitrile is often used as a component of the mobile phase for reverse-phase chromatography. By controlling the concentration and addition method of acetonitrile, the target product can be selectively precipitated.

[0003] Among many existing technologies, Chinese Patent Application CN214654576U discloses a device for large-scale nucleic acid synthesis gas-phase ammonolysis, including an ammonolysis tank; the ammonolysis tank is a cylindrical tank body with an inner cavity, a driving mechanism is arranged at the bottom of the inner cavity of the ammonolysis tank, a clamping mechanism is arranged at the middle position of the inner cavity of the ammonolysis tank, the top of the ammonolysis tank is hinged with a sealed top cover, a spraying mechanism, a pressure gauge and an air inlet mechanism are embedded on the sealed top cover, the pressure gauge is located between the spraying mechanism and the air inlet mechanism, a water pool is arranged at the bottom of the inner cavity of the ammonolysis tank, a plurality of heating plates are arranged on the side wall of the inner cavity of the ammonolysis tank, the heating plates are located in the water pool, and a water outlet pipe with a switch is arranged at the bottom of the water pool.

[0004] However, in this patent application, acetonitrile is sprayed through a spraying disc, which will cause some reagents to be sprayed on the clamping plate, resulting in material waste, increasing costs and also reducing the service life of the equipment. At the same time, the synthesis column is clamped on the clamping holes of the clamping plate and the clamping plate is driven by a rotating motor to rotate in the ammonolysis tank and is not detachable. The taking and placing of the synthesis column can only be carried out individually, which is not convenient and has low efficiency.

[0005] Based on this, the utility model designs an ammonolysis device for nucleic acid production that is convenient to operate to solve the above problems. Summary of the Utility Model

[0006] In view of the above-mentioned drawbacks of the prior art, the utility model provides an ammonolysis device for nucleic acid production that is convenient to operate.

[0007] To achieve the above purposes, the utility model is realized through the following technical solutions:

[0008] A nucleic acid production ammonolysis device convenient for operation, comprising an installation shell assembly. An installation and limiting assembly for fixing a synthesis column is installed inside the installation shell assembly. A rotating motor for rotating the installation and limiting assembly is installed on the installation shell assembly. A quantitative injection assembly for quantitatively injecting ammonolysis liquid and acetonitrile is installed on the installation shell assembly;

[0009] The quantitative injection assemblies are symmetrically arranged in two groups on the left and right. One group of quantitative injection assemblies includes a group of driving electric cylinders, a group of connecting plates, and multiple groups of quantitative assemblies. The driving electric cylinder is connected to the installation shell assembly, the output end of the driving electric cylinder is fixedly connected to the upper end face of the connecting plate, and the connecting plate is connected to the quantitative assemblies.

[0010] Furthermore, the installation shell assembly includes an installation shell, an installation frame, and a closing plate. The lower end of the installation frame is fixedly connected to the upper end face of the installation shell, the inner top end of the installation frame is fixedly connected to the driving electric cylinder. The lower side of the closing plate is hinged to the lower front side of the installation shell, the upper side of the closing plate is locked to the installation shell through a locking mechanism, and a rubber ring is arranged around the closing plate. When the closing plate is closed, it is sealed with the installation shell.

[0011] Furthermore, the bottom end of the rotating motor is fixedly connected to the inner bottom of the installation shell.

[0012] Furthermore, the installation and limiting assembly includes an installation assembly and a limiting assembly. The installation assembly is connected to the rotating motor, the limiting assembly is connected to the installation assembly, and there are two groups of limiting assemblies.

[0013] Furthermore, the installation assembly includes an installation seat and an installation plate. The bottom surface of the installation seat is fixedly connected to the output end of the rotating motor. A card slot for fitting and limiting the sliding connection of the installation plate is opened at the upper end of the installation seat. The installation seat and the installation plate are connected to the limiting assembly, and limiting holes are respectively opened on both sides of the front end of the installation plate.

[0014] Furthermore, the limiting assemblies are symmetrically arranged on the left and right sides of the front end of the installation seat. The limiting assembly includes a limiting ball, a spring, and a protective shell. The upper end of the limiting ball is in contact connection with the installation seat. The circumference of the upper hemisphere of the limiting ball is clamped with the limiting holes opened on the installation plate. The upper end of the limiting ball is slidably connected to the bottom surface of the installation plate. The lower end of the limiting ball is fixedly connected to the upper end of the spring. The lower end of the spring is fixedly connected to the inner bottom of the protective shell. The upper end of the protective shell is fixedly connected to the inner top end of the installation seat.

[0015] Further, the multiple sets of metering components included in the set of metering injection components are arranged in a four-row and two-column array. A set of metering components includes a set of collection bins, a set of pistons, a set of connecting rods, and two sets of one-way valves. The upper end of the collection bin is fixedly connected to the inner top end of the installation shell. The lower end of the collection bin is fixedly connected to the input end of one set of one-way valves. The side of the collection bin is fixedly connected to the output end of the other set of one-way valves. The side one-way valve is connected to the input pipe. The piston is slidably connected to the inner wall of the collection bin. The upper end of the piston is fixedly connected to the lower end of the connecting rod. The connecting rod is slidably connected to the upper end of the collection bin and the upper end of the installation shell. The upper end of the connecting rod is fixedly connected to the lower end surface of the connecting plate.

[0016] Further, the metering component further includes a set of spray heads. The upper end of the spray head is fixedly connected to the output end of the one-way valve at the lower end of the collection bin. The lower end of the spray head is higher than the upper end of the synthesis column.

[0017] Compared with the prior art, the beneficial effects of the present utility model are as follows: 1. It realizes the quantitative injection of ammonolysis liquid and acetonitrile, ensuring that the dosage of reagents used in the reaction is the most efficient and accurate, keeping the reaction in the best reaction conditions all the time. At the same time, the accurate quantitative injection can reduce the waste of reagents and lower the production cost;

[0018] 2. It can place and take the synthesis column in batches, making the operation more convenient. Description of the Drawings

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0020] Figure 1 Isometric view of a nucleic acid production ammonolysis device that is easy to operate according to the present utility model Figure 1 ;

[0021] Figure 2 Is the front view of a nucleic acid production ammonolysis device that is easy to operate according to the present utility model;

[0022] Figure 3 Is the left view of a nucleic acid production ammonolysis device that is easy to operate according to the present utility model;

[0023] Figure 4 Is the sectional view along the Figure 3 A-A direction;

[0024] Figure 5 Is the sectional view along the Figure 3 B-B direction;

[0025] Figure 6 is Figure 4 an enlarged view of part C in

[0026] Figure 7 is Figure 5 an enlarged view of part D in

[0027] The reference numerals in the figure respectively represent:

[0028] 1. Installation shell assembly; 11. Installation shell; 12. Installation bracket; 13. Closing plate; 2. Rotating motor; 3. Installation limit assembly; 31. Installation assembly; 311. Installation seat; 312. Installation plate; 32. Limit assembly; 321. Limit ball; 322. Spring; 323. Protective shell; 4. Quantitative injection assembly; 41. Driving electric cylinder; 42. Connecting plate; 43. Quantitative assembly; 431. Collection bin; 432. Piston; 433. Connecting rod; 434. Check valve; 435. Sprayer; 5. Synthesis column. Specific embodiments

[0029] To make the objectives, technical solutions, and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are some, but not all, of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without making creative efforts fall within the scope of protection of the present utility model.

[0030] The "left", "right", "front", "rear", "upper", and "lower" mentioned in the following description are oriented in the perspective direction of the front view.

[0031] In some embodiments, referring to Figures 1 - 7 of the accompanying drawings of the specification, a nucleic acid production ammonolysis device that is convenient to operate includes an installation shell assembly 1. An installation limit assembly 3 for fixing a synthesis column 5 is installed inside the installation shell assembly 1. A rotating motor 2 for rotating the installation limit assembly 3 is installed on the installation shell assembly 1. A quantitative injection assembly 4 for quantitatively injecting ammonolysis solution and acetonitrile is installed on the installation shell assembly 1;

[0032] Two groups of quantitative injection assemblies 4 are symmetrically arranged on the left and right. One group of quantitative injection assemblies 4 includes one group of driving electric cylinders 41, one group of connecting plates 42, and multiple groups of quantitative assemblies 43. The driving electric cylinders 41 are connected to the installation shell assembly 1. The output end of the driving electric cylinder 41 is fixedly connected to the upper end surface of the connecting plate 42. The connecting plate 42 is connected to the quantitative assembly 43;

[0033] When the utility model is in use, the installation shell assembly 1 is opened, and then the synthesis column 5 is installed on the installation limiting assembly 3 and limited by the installation limiting assembly 3. After completion, the installation shell assembly 1 is closed and sealed. Then, the quantitative injection assembly 4 on the left injects the ammonolysis liquid into the synthesis column 5 located under the quantitative injection assembly 4 on the left in a quantitative manner. After the injection is completed, the rotating motor 2 drives the installation limiting assembly 3 to drive the synthesis column 5 to rotate, so that the synthesis column 5 on the other side without the ammonolysis liquid injection is located under the quantitative injection assembly 4 on the left, and the quantitative injection assembly 4 on the left injects the ammonolysis liquid in a quantitative manner. After the injection is completed, the reaction is carried out. After the reaction is completed, the quantitative injection assembly 4 on the right injects acetonitrile into the synthesis column 5 currently located below for purification. After the injection is completed, the rotating motor 2 drives the installation limiting assembly 3 to drive the synthesis column 5 to rotate, so that the synthesis column 5 on the other side without the acetonitrile injection is located under the quantitative injection assembly 4 on the right and then the acetonitrile is injected in a quantitative manner. After the reaction is completed, the organic gas is evacuated through the air outlet pipe on the installation shell assembly 1, and then the installation shell assembly 1 is opened to take out the synthesis column 5. The quantitative injection of the ammonolysis liquid and acetonitrile is realized, ensuring that the dosage of the reagents used in the reaction is the most efficient and accurate, making the reaction always in the best reaction conditions. At the same time, the accurate quantitative injection can reduce the waste of reagents, lower the production cost, and the synthesis column 5 can be placed and taken in batches, making the operation more convenient.

[0034] The installation shell assembly 1 includes an installation shell 11, an installation frame 12 and a closing plate 13. The lower end of the installation frame 12 is fixedly connected to the upper end surface of the installation shell 11. The inner top end of the installation frame 12 is fixedly connected to the driving electric cylinder 41. The lower side of the closing plate 13 is hinged to the lower front side of the installation shell 11. The upper side of the closing plate 13 is locked with the installation shell 11 through a locking mechanism, and a rubber ring is arranged around the closing plate 13. When the closing plate 13 is closed, it is sealed with the installation shell 11. The locking mechanism is a handle-type tongue lock, and the handle-type tongue lock adopts the existing mature technology;

[0035] When the utility model is in use, the closing plate 13 cooperates with the installation shell 11 to seal the internal reaction space.

[0036] The bottom end of the rotating motor 2 is fixedly connected to the inner bottom of the installation shell 11;

[0037] The installation limiting assembly 3 includes an installation assembly 31 and a limiting assembly 32. The installation assembly 31 is connected to the rotating motor 2, and the limiting assembly 32 is connected to the installation assembly 31. There are two groups of the limiting assemblies 32;

[0038] The installation assembly 31 includes an installation base 311 and an installation plate 312. The bottom surface of the installation base 311 is fixedly connected to the output end of the rotating motor 2. A clamping groove for the installation plate 312 to be limited and slidably connected is opened at the upper end of the installation base 311. The installation base 311 and the installation plate 312 are connected to the limiting assembly 32. Limiting holes are respectively opened on both sides of the front end of the installation plate 312;

[0039] The limiting components 32 are symmetrically arranged on the left and right sides of the front end of the mounting seat 311. The limiting components 32 include limiting balls 321, springs 322 and protective cases 323. The upper ends of the limiting balls 321 are in contact connection with the mounting seat 311. The circumferences of the upper hemispheres of the limiting balls 321 are clamped with the limiting holes formed in the mounting plate 312. The upper ends of the limiting balls 321 are in sliding connection with the bottom surface of the mounting plate 312. The lower ends of the limiting balls 321 are fixedly connected with the upper ends of the springs 322. The lower ends of the springs 322 are fixedly connected with the inner bottom of the protective cases 323. The upper ends of the protective cases 323 are fixedly connected with the inner top ends of the mounting seats 311;

[0040] When the utility model is in use, the synthesis column 5 is clamped into the mounting plate 312. There is a rubber ring on the mounting plate 312 to make the clamping of the synthesis column 5 more stable. Then, the mounting plate 312 with the synthesis column 5 placed is clamped into the mounting seat 311. Limiting holes are formed in the left and right sides of the front end of the mounting plate 312. When the mounting seat 311 is pushed to the end, the spring 322 makes the limiting ball 321 snap upward into the limiting hole of the mounting plate 312 to limit the mounting plate 312. When taking it out, pulling the mounting plate 312 outwards will drive the limiting ball 321 to compress the spring 322, so that the limiting ball 321 is located below the mounting plate 312, and then the mounting plate 312 can be taken out.

[0041] A set of quantitative injection components 4 includes multiple sets of quantitative components 43 arranged in a four-row and two-column array. A set of quantitative components 43 includes a set of collection bins 431, a set of pistons 432, a set of connecting rods 433 and two sets of one-way valves 434. The upper ends of the collection bins 431 are fixedly connected with the inner top ends of the mounting shells 11. The lower ends of the collection bins 431 are fixedly connected with the input ends of a set of one-way valves 434. The sides of the collection bins 431 are fixedly connected with the output ends of the other set of one-way valves 434. The side one-way valves 434 are connected to the input pipes. The pistons 432 are in sliding connection with the inner walls of the collection bins 431. The upper ends of the pistons 432 are fixedly connected with the lower ends of the connecting rods 433. The connecting rods 433 are in sliding connection with the upper ends of the collection bins 431 and the upper ends of the mounting shells 11. The upper ends of the connecting rods 433 are fixedly connected with the lower end surfaces of the connecting plates 42;

[0042] The quantitative component 43 further includes a set of nozzles 435. The upper ends of the nozzles 435 are fixedly connected with the output ends of the one-way valves 434 at the lower ends of the collection bins 431. The lower ends of the nozzles 435 are higher than the upper ends of the synthesis columns 5;

[0043] When the utility model is in use, the driving electric cylinders 41 on both sides operate respectively. The connecting plate 42 is lifted by the driving electric cylinder 41. The connecting plate 42 drives the connecting rod 433, and the connecting rod 433 drives the piston 432 to move upward in the collection bin 431, so that a negative pressure is formed inside the collection bin 431, and the reaction reagent ammonolysis solution or acetonitrile is inhaled through the side one-way valve 434. When the required volume is inhaled, the driving electric cylinder 41 drives the connecting plate 42 to descend. The connecting plate 42 drives the connecting rod 433, and the connecting rod 433 drives the piston 432 to move downward in the collection bin 431, and the reaction reagent flows into the spray head 435 through the lower one-way valve 434. The spray head 435 injects the reaction reagent into the synthesis column 5, realizing the quantitative injection of the ammonolysis solution and acetonitrile, ensuring that the dosage of the reagent used in the reaction is the most efficient and accurate, and keeping the reaction in the best reaction conditions all the time.

[0044] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements will not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A nucleic acid production ammonolysis device convenient for operation, comprising an installation shell assembly (1), characterized in that: An installation limit component (3) for fixing a synthesis column (5) is installed inside the installation shell component (1), a rotation motor (2) for rotating the installation limit component (3) is installed on the installation shell component (1), and a quantitative injection component (4) for quantitatively injecting ammonolysis liquid and acetonitrile is installed on the installation shell component (1); Two groups of the quantitative injection components (4) are symmetrically arranged left and right. One group of the quantitative injection components (4) includes a group of driving electric cylinders (41), a group of connecting plates (42) and multiple groups of quantitative components (43). The driving electric cylinder (41) is connected to the installation shell component (1), the output end of the driving electric cylinder (41) is fixedly connected to the upper end surface of the connecting plate (42), and the connecting plate (42) is connected to the quantitative component (43).

2. The nucleic acid production ammonolysis device facilitating operation according to claim 1, wherein The installation shell component (1) includes an installation shell (11), an installation frame (12) and a closing plate (13). The lower end of the installation frame (12) is fixedly connected to the upper end surface of the installation shell (11), the inner top end of the installation frame (12) is fixedly connected to the driving electric cylinder (41). The lower side of the closing plate (13) is hinged to the lower front side of the installation shell (11), the upper side of the closing plate (13) is locked to the installation shell (11) through a locking mechanism, and a rubber ring is arranged around the closing plate (13). When the closing plate (13) is closed, it is sealed with the installation shell (11).

3. The ammoniaolysis device for nucleic acid production that is convenient to operate according to claim 2, wherein The bottom end of the rotation motor (2) is fixedly connected to the inner bottom of the installation shell (11).

4. The nucleic acid production ammonolysis device convenient for operation according to claim 3, characterized in that, The installation limit component (3) includes an installation component (31) and a limit component (32). The installation component (31) is connected to the rotation motor (2), the limit component (32) is connected to the installation component (31), and there are two groups of the limit components (32).

5. The nucleic acid production ammonolysis device convenient for operation according to claim 4, wherein, The installation component (31) includes an installation seat (311) and an installation plate (312). The bottom surface of the installation seat (311) is fixedly connected to the output end of the rotation motor (2). A clamping groove for limiting and sliding connection with the installation plate (312) is formed at the upper end of the installation seat (311). The installation seat (311) and the installation plate (312) are connected to the limit component (32), and limiting holes are respectively formed on both sides of the front end of the installation plate (312).

6. The ammoniaolysis device for nucleic acid production that is convenient to operate according to claim 5, wherein, The limit components (32) are symmetrically arranged on the left and right sides of the front end of the installation seat (311). The limit component (32) includes a limit ball (321), a spring (322) and a protective shell (323). The upper end of the limit ball (321) is in contact connection with the installation seat (311). The circumference of the upper hemisphere of the limit ball (321) is clamped with the limiting holes formed on the installation plate (312). The upper end of the limit ball (321) is slidably connected to the bottom surface of the installation plate (312). The lower end of the limit ball (321) is fixedly connected to the upper end of the spring (322). The lower end of the spring (322) is fixedly connected to the inner bottom of the protective shell (323). The upper end of the protective shell (323) is fixedly connected to the inner top end of the installation seat (311).

7. The nucleic acid production ammonolysis device convenient for operation according to claim 6, characterized in that, The multiple groups of metering components (43) included in the set of metering injection components (4) are arranged in a four-row and two-column array. A group of metering components (43) includes a group of collection bins (431), a group of pistons (432), a group of connecting rods (433), and two groups of one-way valves (434). The upper end of the collection bin (431) is fixedly connected to the inner top end of the mounting shell (11). The lower end of the collection bin (431) is fixedly connected to the input end of a group of one-way valves (434). The side surface of the collection bin (431) is fixedly connected to the output end of the other group of one-way valves (434). The side one-way valve (434) is connected to the input pipe. The piston (432) is slidably connected to the inner wall of the collection bin (431). The upper end of the piston (432) is fixedly connected to the lower end of the connecting rod (433). The connecting rod (433) is slidably connected to the upper end of the collection bin (431) and the upper end of the mounting shell (11). The upper end of the connecting rod (433) is fixedly connected to the lower end surface of the connecting plate (42).

8. The nucleic acid production ammonolysis device facilitating operation according to claim 7, characterized in that, The metering component (43) further includes a group of spray heads (435). The upper end of the spray head (435) is fixedly connected to the output end of the one-way valve at the lower end of the collection bin (431). The lower end of the spray head (435) is higher than the upper end of the synthesis column (5).

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