Liquid drop generating device

Abstract

The utility model provides a liquid drop generating device which comprises a liquid drop generating chip and a packaging part used for sealing and fixing the chip. The oil phase storage tank, the sample storage tank and the biological reagent storage tank are directly arranged on the liquid drop generation chip, the air inlet holes are respectively connected above the storage tanks, and liquid drops can be directly generated by feeding fluid in the storage tanks into the liquid drop generator through air pressure, so that the waste of biological reagents is greatly reduced. The liquid drop generator comprises an oil phase flow channel, a sample flow channel and a biological reagent flow channel, and one or more sections of arc-shaped flow channels are arranged on the oil phase flow channel, the sample flow channel and the biological reagent flow channel respectively to reduce blockage and control the flow speed. A buffer tank is arranged at a sample and biological reagent injection port and used for pre-arrangement of samples and biological reagents. A buffer groove is formed in an oil phase sample inlet, so that the flow resistance is increased, and the controllability of the oil phase flow velocity is improved. According to the utility model, the waste of biological reagents can be reduced, the sealing is realized, the structure is simple, liquid drops can be quickly generated, and the effective liquid drop proportion is high.

Description

Technical Field

[0001] The utility model relates to the field of microfluidic control, specifically to a droplet generation chip for generating multi-particle droplets, and in particular to a multi-particle droplet generator with an oil phase flow channel, a sample flow channel and a biological reagent flow channel. Background Art

[0002] Microfluidic chips, also known as lab-on-a-chips, integrate basic operational units such as sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes onto a micron-scale chip, automating the entire analysis process. Compared to traditional laboratories, microfluidic chips offer controllable liquid flow, minimal sample and reagent consumption, and analysis speeds that are tenfold or even a hundredfold higher. They can simultaneously analyze hundreds of samples in a matter of minutes or even less, and can perform the entire sample pretreatment and analysis process online, significantly reducing experimental time and space costs. Microfluidic chips are used in numerous fields, including chemistry, biology, and medicine.

[0003] Microfluidics can be used to control microparticles. When two immiscible liquids (oil and water) flow through microfluidic channels, the liquid / liquid interfacial tension and shear forces cause one phase of the fluid to form a highly uniform, discontinuous flow, or droplets. In the life sciences, microfluidic droplets can be used for high-throughput drug screening, toxicity testing, and antibody affinity analysis; for single-cell proteomics and genomics analysis, to study heterogeneity within cell populations; and for high-throughput directed protein evolution.

[0004] Multi-particle droplets refer to droplets containing two or more particles, including cells, encoded microspheres, and so on. To address issues such as flow velocity and resistance control, pipeline blockage, and low effective flux or a low percentage of effective droplets, existing technologies often extend the length of the oil phase flow channel, sample flow channel, and bioreagent flow channel and design them into multiple continuous U-shaped flow channels with different turning radii. Furthermore, the sample injection port is not provided on the chip. This not only leads to a serious waste of certain precious and trace bioreagents, but also fails to effectively control the flow rate, and the percentage of effective droplets produced by the droplet generator remains low.

[0005] Therefore, there is an urgent need to find a droplet generator that can not only reduce the waste of biological reagents, but also effectively control the flow resistance and flow rate of each flow channel, and is not easy to clog the flow channel, thereby increasing the proportion of effective droplets. Utility Model Content

[0006] In order to solve the above problems, the present invention provides a droplet generating device, which is a sealing device that wraps a droplet generating chip with an upper cover and a base. An oil phase storage tank, a sample storage tank and a biological reagent storage tank are set on the droplet generating chip, and an air inlet is connected above each storage tank. After each fluid is sent into the droplet generator by air pressure, droplets can be directly generated, which greatly reduces the waste of biological reagents. The droplet generator includes an oil phase flow channel, a sample flow channel and a biological reagent flow channel. The present invention provides a section of arc-shaped flow channel on the sample flow channel and the biological reagent flow channel to prevent samples and biological specimens from being blocked in the flow channel; a buffer tank is provided on the sample injection port and the biological reagent injection port for pre-arrangement of samples and biological reagents. Three sections of arc-shaped flow channels are provided on the oil phase flow channel, and a buffer tank is provided at the lower end of the oil phase injection port to increase the flow resistance, increase the controllability of the oil phase flow rate, and prevent the oil phase from infiltrating into the water phase due to excessive flow rate. The utility model not only reduces the waste of biological reagents and is sealed and simple in structure, but also effectively controls the flow resistance and flow rate of samples, oil phase and biological reagents, is not easy to clog the sample flow channel, quickly generates droplets and has a higher proportion of effective droplets.

[0007] The utility model provides a droplet generation device, comprising a droplet generation chip, wherein the droplet generation chip is provided with a storage tank and a droplet generator, wherein the storage tank comprises an oil phase storage tank, a biological reagent storage tank and a sample storage tank, which are respectively used to store the oil phase, the biological reagent and the sample, wherein the biological reagent can be selected from one or more of cells, nucleic acids, proteins, antibodies or antibody fragments, and the sample can be selected from one or more of coded microspheres, reporter cells, magnetic beads and substrates; and the bottom of the storage tank is connected to the droplet generator.

[0008] Furthermore, the droplet generation device also includes a packaging component for encapsulating the droplet generation chip, the packaging component includes a packaging fixture, and the packaging fixture includes an upper cover and a base. The upper cover and the base are combined into a fixture with a cavity inside, so that the microfluidic chip placed in the cavity is fixed and sealed. The packaging component provides a sealed working environment for the droplet generation chip.

[0009] Furthermore, in the droplet generating device, the sample storage tank is covered with a pressing plate, and the pressing plate is provided with an air inlet hole for introducing gas to force the sample in the sample storage tank to enter the droplet generator.

[0010] Furthermore, the air inlet is connected to the silicone cap of the upper cover through a pipe, and the silicone cap is equipped with a hose connected to an external pipe for connecting the gas.

[0011] Furthermore, the packaging component also includes a packaging buckle plate, which closes and fixes the upper cover and the pressure plate from one side.

[0012] Further, in the droplet generation device, an oil phase flow channel, a biological reagent flow channel, and a sample flow channel are provided on the droplet generator. Sampling holes are respectively provided at the bottoms of the oil phase storage tank, the biological reagent storage tank, and the sample storage tank, and fluid communication with the oil phase flow channel, the biological reagent flow channel, and the sample flow channel is achieved through the sampling holes.

[0013] A droplet generation device, wherein the length of the oil phase storage tank is 300 - 400 times the width of the oil phase flow channel, the width is 200 - 300 times the width of the oil phase flow channel, and the depth is 150 - 400 times the depth of the oil phase spherical flow channel.

[0014] Further, in the droplet generation device, the biological reagent storage tank and flow channel and the sample storage tank and flow channel are of the same size and are axially symmetric. The length of the storage tank is 150 - 200 times the width of the flow channel, the width is 100 - 150 times the width of the flow channel, and the depth is 150 - 400 times the depth of the flow channel.

[0015] Further, in the droplet generation device, the oil phase sampling port further includes a buffer tank whose shape gradually becomes larger from top to bottom and then quickly retracts downward. The biological reagent sampling port and the sample sampling port further include buffer tanks whose shapes are conical from top to bottom.

[0016] A buffer tank whose shape gradually becomes larger from top to bottom and then quickly retracts downward is provided at the oil phase sampling port. When the oil phase enters the buffer tank, since the width of the buffer tank gradually increases and the flow resistance gradually decreases, the oil phase will first fill the buffer tank. However, the rapid retraction of the lower end of the sampling port causes the oil phase to flow out of the sampling port and needs to overcome a suddenly increased flow resistance, which is equivalent to adding an extra control for the oil phase, increasing the flow resistance, significantly increasing the controllability of the oil phase flow rate, increasing the resistance to the reverse infiltration of the oil phase into the water phase, and significantly reducing the possibility of the reverse infiltration of the oil phase into the water phase.

[0017] Taking a cell sample as an example of the biological reagent and a coded microsphere as an example of the sample. Some cell samples are very precious and rare. Coupled with the small volume of cells and the large volume of coded microspheres, which occupy a relatively large volume in the coded microsphere suspension, the cell suspension is almost equivalent to the aqueous phase. If only the flow rate of the cell suspension is controlled to increase the proportion of effective droplets, it is difficult to control. However, the coded microspheres are relatively low in price and large in quantity, making it easier to control the flow rate. In order to ensure that each cell can exactly encounter a coded microsphere when flowing out and be successfully encapsulated into a droplet, we not only need to control the flow rate of the cell suspension, but more importantly, control the spacing between each coded microsphere. This can make full use of the cell sample, avoid wasting expensive cell samples, and increase the proportion of effective droplets. The diameter of the coded microspheres in the coded microsphere suspension is relatively large, and the proportion of each coded microsphere in the coded microsphere suspension is also large. The flow situation of each coded microsphere in the flow channel will affect the flow resistance of the coded microsphere suspension. Therefore, the flow resistance can be changed by changing the flow channel structure, thereby strengthening the control of the flow situation of the coded microspheres. However, it is not easy to control the distance between each coded microsphere only by air pressure and an arc-shaped flow channel. Therefore, a buffer tank with a tapered shape that uniformly shrinks from top to bottom is added at the sample inlet and the biological reagent inlet respectively. When the coded microsphere suspension or the cell suspension enters the buffer tank, due to the width of the buffer tank being wider at the top and narrower at the bottom, the flow resistance gradually increases, and the flow resistance at the bottom of the inlet is the largest. Therefore, at first, the coded microspheres and cells are not easily discharged from the bottom of the inlet, but are pre-aligned in the buffer tank. When the pressure on the coded microspheres and cells is sufficient to overcome the increased flow resistance at the lower end of the inlet, the coded microspheres and cells will flow out from the lower end of the buffer tank with the fluid one by one.

[0018] Furthermore, in the droplet generation device described above, the biological reagent flow channel and the sample flow channel include an arc-shaped flow channel, and the flow channel includes a section of circular arc-shaped flow channel. Biological reagents such as cell suspensions and the oil phase can be filtered through a filter membrane to ensure that they do not contain impurities such as dust and microfibers, and generally will not cause blockage of the flow channel. However, for samples such as the coded microspheres in the coded microsphere suspension, due to their relatively large diameter, generally about 50 μm, it is impossible to use a filter membrane for filtration, resulting in some dust, microfibers and other impurities remaining in the coded microsphere suspension. The present invention solves the problem of blockage by impurities such as dust and microfibers by adding a section of arc-shaped flow channel to the sample flow channel, reducing the risk of blockage of the sample flow channel, thereby ensuring that the sample flow channel can operate smoothly for a long time.

[0019] Furthermore, in the droplet generation device described above, the oil phase flow channel includes a first oil phase flow channel and a second oil phase flow channel and is axisymmetric. The first oil phase flow channel and the second oil phase flow channel include arc-shaped flow channels, and the arc-shaped flow channel includes three sections of circular arc-shaped flow channels. The purpose of setting three arc-shaped bends is to increase the flow resistance of the oil phase and improve the controllability of the oil phase flow rate.

[0020] Furthermore, in the droplet generating device, the sample flow channel and the biological reagent flow channel form a first Y-shaped flow channel. The output channel of the first Y-shaped flow channel and the oil phase flow channel form a second cross-shaped flow channel. There is a certain distance between the first Y-shaped flow channel and the second cross-shaped flow channel. The width and / or depth of the outlet section flow channel of the second cross-shaped flow channel is greater than the width and / or depth of the outlet end. Devices for indicating channels are marked on both sides of the first Y-shaped flow channel to distinguish different flow channels and facilitate setting conditions. Devices for measuring the flow rate and the number of droplets are marked on one side of the second cross-shaped flow channel, which can be directly connected to external instruments to display the flow rate and the number of droplets.

[0021] Research has shown that the length of the output channel between the first Y-shaped flow channel and the second cross-shaped flow channel must be maintained within a certain range to better play the role of the oil phase shearing and wrapping to form effective droplets. This is mainly because there needs to be a certain time interval between each time the oil phase shears and wraps to form droplets. The distance between the first Y-shaped flow channel and the second cross-shaped flow channel needs to be as matched as possible with the time interval between each time the oil phase shears and wraps to form droplets, which can further improve the efficiency of generating effective droplets.

[0022] Research has shown that when the width and / or depth of the outlet section flow channel of the second cross-shaped flow channel is greater than the width and / or depth of the outlet end, the generated droplets can flow out faster. This is mainly because the increase in width and / or depth reduces the flow resistance and speeds up the flow rate. Although the increase in width and / or depth reduces the flow resistance and speeds up the flow rate, the flow rate cannot be too fast, otherwise it will cause difficulties in subsequent droplet sorting.

[0023] The present utility model provides a droplet generator, which has the following beneficial effects:

[0024] 1. An oil phase storage tank, a sample storage tank, and a biological reagent storage tank are provided on the droplet generation chip. An oil phase injection port, a sample injection port, and a biological reagent injection port are respectively connected below each storage tank. Each injection port is respectively connected to an oil phase flow channel, a sample flow channel, and a biological reagent flow channel to directly generate droplets, greatly reducing the waste of biological reagents.

[0025] 2. The present utility model is a sealed device. An upper cover, a base, and a packaging buckle plate are combined to form a fixture with a cavity inside, so that the microfluidic chip placed in the cavity is fixed and sealed. Three air holes are provided and respectively connected to the oil phase storage tank, the sample storage tank, and the biological reagent storage tank, and the fluid is sent into the injection port through air pressure.

[0026] 3. The present utility model is provided with an arc-shaped flow channel on each of the sample flow channel and the biological reagent flow channel to prevent the sample and the biological reagent from being blocked in the flow channel; buffer grooves are provided at the sample inlet and the biological reagent inlet for pre-aligning the sample and the biological reagent. Three arc-shaped flow channels are provided on the oil phase flow channel and a buffer groove is provided at the lower end of the oil phase inlet to increase the flow resistance and the controllability of the oil phase flow rate, and prevent the oil phase from infiltrating into the water phase due to too fast flow rate.

[0027] 4. The present utility model can not only reduce the waste of biological reagents, but also has a sealed and simple structure, can generate droplets quickly and has a higher proportion of effective droplets. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is a schematic diagram of the external structure of the droplet generation device in Example 1;

[0029] Figure 2 is a schematic diagram of the overall structure of the droplet generation device in Example 1;

[0030] Figure 3 is a schematic diagram of the top view structure of the droplet generation chip in Example 1;

[0031] Figure 4 is a schematic diagram of the bottom view structure of the droplet generator in Example 1;

[0032] Figure 5 is a schematic diagram of the structure of the first Y-shaped flow channel and the second cross-shaped flow channel in Example 1. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0033] The preferred embodiments of the present utility model will be further described in detail below with reference to the accompanying drawings. It should be noted that the following embodiments are intended to facilitate the understanding of the present utility model and do not impose any limitation on it. The raw materials and equipment used in the specific embodiments of the present utility model are all known products and are obtained by purchasing commercially available products.

[0034] Example 1 Droplet Generation Device Provided by the Present Utility Model

[0035] The schematic diagram of the droplet generator provided in this embodiment is as Figures 1 to 5 shown, where Figure 1 is a schematic diagram of the external structure of the droplet generation device; Figure 2 is a schematic diagram of the overall structure of the droplet generation device; Figure 3 is a schematic diagram of the top view structure of the droplet generation chip; Figure 4 is a schematic diagram of the bottom view structure of the droplet generator; Figure 5 is a schematic diagram of the structure of the first Y-shaped flow channel and the second cross-shaped flow channel.

[0036] As Figures 1 to 2 shown, Figure 1Schematic diagram of the external structure of the droplet generation device. The overall structural modules are as Figure 2 shown. The upper cover 2, the base 6, the sealing buckle plate 8 and the pressing plate 9 are combined to form a fixture with a cavity inside, so as to fix and seal the droplet generation chip 7 placed in the cavity. The storage tank 3 is a sample storage tank, the storage tank 4 is an oil phase storage tank, and the storage tank 5 is a biological reagent storage tank. These three sample storage tanks are respectively connected to three air inlets 10 on the pressing plate 9. The air inlets 10 are respectively connected to three silicone caps 1 through the conduits in the upper cover 2. A hose is provided inside the silicone cap 1 and is externally connected to a gas pipeline for introducing gas.

[0037] The structure of the droplet generation chip 7, which is the core component of the entire droplet generation device, is as Figure 3 shown. Below the sample storage tanks 3, 4, and 5, there are respectively a sample injection port 12, an oil phase injection port 11, and a biological reagent injection port 13. 14 and 15 are respectively a sample reserve storage tank and a biological reagent reserve storage tank. The length of the oil phase storage tank is 17280 μm, the width is 10170 μm, and the depth is 8000 μm; the lengths of the biological reagent storage tank and the sample storage tank are 9410 μm, the width is 8280 μm, and the depth is 8000 μm.

[0038] As Figure 4 shown, Figure 4 Schematic diagram of the bottom view of the droplet generator. The droplet generator includes a first oil phase flow channel 19, a second oil phase flow channel 20, a sample flow channel 22, and a biological reagent flow channel 21. In this Example 1, the lengths of the first oil phase flow channel 19 and the second oil phase flow channel 20 are 23920 μm, the width is 50 μm, and the depth is 50 / 20 μm; the lengths of the sample flow channel 22 and the biological reagent flow channel 21 are 9830 μm, the width is 60 μm, and the depth is 50 / 20 μm. When the depth of the three flow channels is 50 μm, droplets with a diameter of about 50 μm are generated; when the depth of the three flow channels is 20 μm, droplets with a diameter of about 30 μm are generated.

[0039] An oil-phase buffer tank 16 is arranged below the oil-phase sampling port 11. Its shape gradually enlarges from top to bottom first and then quickly retracts downward. The width at the widest part of the oil-phase buffer tank 18 is 1000 μm, the depth at the deepest part is 200 μm, and the length is 100 μm. When the oil phase enters the buffer tank 16 after passing through the oil-phase sampling port 11, since the width of the buffer tank first increases and then rapidly decreases, the flow resistance of the oil phase will slowly decrease and then rapidly increase. This is equivalent to adding an extra control to the oil phase, increasing the flow resistance, significantly increasing the controllability of the oil-phase flow rate, increasing the resistance to the reverse infiltration of the oil phase into the water phase, and significantly reducing the possibility of the reverse infiltration of the oil phase into the water phase. A sample buffer tank 18 and a biological reagent buffer tank 17 are respectively arranged below the sample sampling port 12 and the biological reagent sampling port 13. Their shapes are conical from top to bottom with a smooth interface. The width at the widest part of the sample buffer tank 18 and the biological reagent buffer tank 17 is 1000 μm, the depth at the deepest part is 50 μm, and the length is 100 μm. The sample suspension (taking the sample as the coded microspheres as an example) and the cell suspension (taking the biological reagent as the cells as an example) enter the sample buffer tank 18 and the biological reagent buffer tank 17 respectively after passing through the sample sampling port 10 and the biological reagent sampling port 11. Since the buffer tanks are conical with a larger top and a smaller bottom, the flow resistance of the coded microsphere suspension and the cell suspension in the buffer tanks gradually increases from top to bottom, and the coded microspheres and the cell suspension will be pre-arranged in the buffer tanks, increasing the controllability of the flow rates of the coded microsphere suspension and the cell suspension. Three arc-shaped flow channels 23, 25, 27 are arranged on the first oil-phase flow channel 19, and three arc-shaped flow channels 24, 26, 28 are arranged on the second oil-phase flow channel 20 to increase the oil-phase flow resistance to control the flow rate. An arc-shaped flow channel 30 and 29 are respectively added to the sample flow channel 22 and the biological reagent flow channel 21 to avoid blockage and improve the droplet generation and preparation efficiency. Biological reagents such as cell suspensions and the oil phase can both be filtered through the filter membrane to ensure that they do not contain impurities such as dust and microfibers, and generally will not cause blockage of the flow channels. However, the coded microspheres in the sample such as the coded microsphere suspension have a relatively large diameter, generally about 50 μm, so they cannot be filtered through the filter membrane, resulting in some dust, microfibers and other impurities remaining in the coded microsphere suspension. In this Example 1, an arc-shaped flow channel 30 is arranged on the sample flow channel 22 to solve the blockage problem of impurities such as dust and microfibers. Due to this arc-shaped flow channel, the dust, microfibers and other impurities will all be washed away at this flow rate and cannot form a pile at the turning point, reducing the risk of blockage of the sample flow channel, thus ensuring that the sample flow channel can operate smoothly for a long time.

[0040] Such as Figure 5As shown, the sample flow channel 22 and the biological reagent flow channel 21 converge into the first Y-shaped flow channel 31, and the Y-shaped flow channel 31, the first oil phase flow channel 19, and the second oil phase flow channel 20 converge into the second cross-shaped flow channel 32. Taking cells as an example of the biological reagent and coded microspheres as an example of the sample, cells generally have a small volume and a small proportion in the cell suspension, so the cell suspension is equivalent to the aqueous phase. When a cell passes through the Y-shaped flow channel 31, it just encounters a coded microsphere. When the two kinds of microparticles pass through the cross-shaped flow channel 32 together, they are sheared into a droplet by the first oil phase flow channel 19 and the second oil phase flow channel 20. Each droplet contains a coded microsphere and a cell. There needs to be a certain distance between the first Y-shaped flow channel 31 and the second cross-shaped flow channel 32 because there needs to be a certain time interval between the shearing and wrapping of the oil phase to form droplets to further improve the efficiency of generating effective droplets. In this Example 1, the distance between the first Y-shaped flow channel 31 and the second cross-shaped flow channel 32 is 230 μm. When the width of the outlet section flow channel 33 of the second cross-shaped flow channel is greater than the width of the outlet end, more effective droplets can be obtained more efficiently, but the flow rate cannot be too fast, otherwise it will cause difficulties in subsequent droplet sorting. In this example, the width of the outlet section flow channel 33 of the second cross-shaped flow channel is 120 μm. Devices 34 and 35 are provided on both sides of the Y-shaped flow channel 31, which are used to indicate the flow channel 22 and the biological reagent flow channel 21 respectively, facilitating users to distinguish the two flow channels and set conditions. A device 36 is provided beside the outlet section flow channel 33, which can measure the flow rate and quantity of the droplets in the outlet section. The devices 34, 35, and 36 can be externally connected to an instrument to display data.

[0041] Although the present utility model is disclosed as above, the present utility model is not limited thereto. It can be extended according to its application scope in the field of microfluidics. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the present utility model. Therefore, the protection scope of the present utility model should be subject to the scope defined by the claims.

Claims

1. A droplet generating device, characterized in that, It includes a droplet generation chip, which is provided with a storage tank and a droplet generator. The storage tank includes an oil phase storage tank, a sample storage tank and a biological reagent storage tank, which are respectively used to store the oil phase, the sample and the biological reagent; the bottom of the storage tank is connected to the droplet generator.

2. The droplet generation device according to claim 1, characterized in that, The storage tank is covered with a pressure plate, which is provided with an air inlet hole for introducing gas to force the fluid in the storage tank to enter the droplet generator.

3. The droplet generating device according to claim 2, wherein The droplet generator is provided with an oil phase flow channel, a biological reagent flow channel and a sample flow channel. The bottoms of the oil phase storage tank, the biological reagent storage tank and the sample storage tank are respectively provided with injection holes, and fluid communication is achieved with the oil phase flow channel, the biological reagent flow channel and the sample flow channel through the injection holes.

4. The droplet generation device according to claim 3, characterized in that, The length of the oil phase storage tank is 300-400 times the width of the oil phase flow channel, the width is 200-300 times the width of the oil phase flow channel, and the depth is 150-400 times the depth of the oil phase ball flow channel.

5. The droplet generation device according to claim 4, characterized in that, The biological reagent storage tank and flow channel are of the same size as the sample storage tank and flow channel and are axially symmetrical. The length of the storage tank is 150 to 200 times the width of the flow channel, the width is 100 to 150 times the width of the flow channel, and the depth is 150 to 400 times the depth of the flow channel.

6. The droplet generating device according to claim 5, characterized in that, The oil phase injection port also includes a buffer groove whose shape gradually expands from top to bottom and then shrinks downward rapidly. The biological reagent injection port and the sample injection port also include a buffer groove whose shape is tapered from top to bottom.

7. The droplet generation device according to claim 6, wherein, The sample flow channel and the biological reagent flow channel constitute a first Y-shaped flow channel, and the output channel of the first Y-shaped flow channel and the oil phase flow channel constitute a second cross-shaped flow channel; both sides of the first Y-shaped flow channel are marked with channel indication devices, and one side of the second cross-shaped flow channel is marked with flow rate and droplet number measuring devices.

8. The droplet generating device according to claim 7, wherein, It also includes a packaging component for packaging the droplet generation chip, the packaging component includes a packaging fixture, and the packaging fixture includes an upper cover and a base. The upper cover and the base are combined to form a fixture with a cavity inside, so that the microfluidic chip placed in the cavity is fixed and sealed.

9. The droplet generation device according to claim 8, wherein, The upper cover is provided with a pipeline connection air hole and a silicone cap. The silicone cap is equipped with a hose that can be connected to an external pipeline for connecting and introducing gas.

10. The droplet generation device according to claim 9, characterized in that, The packaging component also includes a packaging buckle plate, which closes and fixes the upper cover and the pressing plate from one side.

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