Device for studying element transmission of arbuscular mycorrhizal fungi hyphae

Abstract

The utility model relates to the technical field of soil microorganisms, in particular to a device for studying element transfer of arbuscular mycorrhizal fungus hyphae, which comprises a planting box, a bottom plate of the planting box is of a plane structure, and an isolating device is arranged in the planting box to form two planting chambers in the planting box. The isolation device comprises two partition plates which are horizontally arranged at intervals, the partition plates are connected with the bottom plate and the two opposite side plates of the planting box, the middles of the partition plates are hollowed out, the hollowed-out portions are covered with first nylon nets in a sealed mode, the first nylon nets are used for preventing root systems of plants from penetrating through and allowing hyphae to penetrate through, and the bottom plate of the planting box is further provided with a water leakage part located between the two partition plates. When the device is used, arbuscular mycorrhizal fungi and heavy metal can be applied to any one of the two planting chambers without limitation, and meanwhile on the premise that it is ensured that the heavy metal cannot enter the other planting chamber from one planting chamber along with water, the distance between the two partition plates between the two planting chambers is prevented from being increased. And miniaturization of the whole device is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of soil microorganisms, and particularly relates to a device for studying the element transfer of arbuscular mycorrhizal fungal hyphae. Background Art

[0002] Arbuscular mycorrhizal fungi are the most widely distributed microorganisms in the soil that are obligately symbiotic with plants. In grassland, forest and farmland ecosystems, more than 80% of plants can form a mutually beneficial symbiotic relationship with arbuscular mycorrhizal fungi. Arbuscular mycorrhizal fungi are one of the most important microbial functional groups in the soil ecosystem, and play an important regulatory role in the nutrient cycling, productivity and stability of vegetation. Among them, the hyphal network formed by arbuscular mycorrhizal fungi can help plants absorb soil nutrients such as heavy metal cadmium, nitrogen, phosphorus, and carbon, improve the water status of plants, enhance the drought resistance of plants, and enhance the disease resistance of plants.

[0003] At present, a device for studying the transfer of heavy metal elements between plant individuals disclosed in the existing patent publication number CN217591615U includes two spaced-apart planting chambers and a transfer chamber connecting the two planting chambers. The planting chambers and the transfer chamber are separated by a partition with holes and a nylon net provided on one side of the partition. The bottom plate of one of the planting chambers is higher than or flush with the top plate of the other planting chamber. The device for studying the transfer of heavy metal elements between plant individuals calculates the absolute value of the transfer of heavy metal between plant individuals by studying the heavy metal content in the plants in the higher planting chamber, which facilitates the research of scientific researchers.

[0004] However, the device disclosed in the above patent has at least the following defects:

[0005] 1. There is a height difference between the two planting chambers, and the transfer chamber between the two planting chambers is inclined. In order to prevent water from entering the lower planting chamber from the higher planting chamber and bringing heavy metal elements into the lower planting chamber, which may affect the test results, when using this device, the hyphae and heavy metals can only be applied in the lower planting chamber, resulting in limited use of the device and affecting the use of the device.

[0006] 2. There is a height difference between the bottom plates of the two planting chambers, and the transfer chamber between the two planting chambers is inclined, resulting in an increased distance between the two planting chambers, which is not conducive to the miniaturization of the entire device.

[0007] 3. Support legs need to be provided at the bottom of the higher planting chamber and extended to be flush with the bottom of the lower planting chamber in order to place the device on a horizontal plane for use, resulting in an increase in the cost of the entire device, and the support legs will also increase the volume of the entire device, which is not conducive to the miniaturization of the entire device. Summary of the Utility Model

[0008] One of the purposes of the present utility model is to provide a device for studying the transfer of elements by arbuscular mycorrhizal fungal hyphae, aiming to solve the technical problems that when the existing device for studying the transfer of heavy metal elements between plant individuals by hyphae is in use, the heavy metals can only be applied in the lower planting chamber, which affects the use of the device. At the same time, there is a height difference between the bottom plates of the two planting chambers, resulting in an inclined transfer chamber between the two planting chambers, thereby increasing the distance between the two planting chambers and being unfavorable for the miniaturization of the entire device.

[0009] To achieve the above purpose, the present utility model provides a device for studying the transfer of elements by arbuscular mycorrhizal fungal hyphae, including a planting box. The bottom plate of the planting box is of a planar structure. At least one isolation device is arranged in the planting box, and one isolation device forms two planting chambers in the planting box. The isolation device includes two partitions arranged at a horizontal interval. The partitions are hermetically connected to the bottom plate of the planting box, and the partitions are also hermetically connected to the relatively arranged first side plate and second side plate of the planting box. The middle part of the partition is hollowed out, and the hollowed-out part is covered with a first nylon mesh. The first nylon mesh is used to block the penetration of plant roots and allow the penetration of hyphae. A water leakage part located between the two partitions is also opened on the bottom plate of the planting box, and the water leakage part penetrates the opposite surfaces of the bottom plate of the planting box.

[0010] Further, a drainage part is arranged at the bottom of the planting chamber, and the drainage part penetrates the opposite surfaces of the bottom plate of the planting box. A second nylon mesh is fixedly covered on the drainage part. The second nylon mesh is used to block the penetration of plant roots and allow the penetration of hyphae.

[0011] Further, the drainage part includes a plurality of drainage holes arranged in an array.

[0012] Further, the bottom between the two partitions is hollowed out to form the water leakage part.

[0013] Further, a water leakage hole penetrating the opposite surfaces of the bottom plate of the planting box is opened at the bottom between the two partitions, and the water leakage hole is configured as the water leakage part.

[0014] Further, the distance between the two partitions is set as L, and the relationship satisfied by L is: 0.5 ≤ L ≤ 1.5 cm.

[0015] Further, acrylic support seats are arranged at the four corner positions of the bottom plate of the planting box.

[0016] Further, the first side plate or the second side plate of the planting box is a transparent acrylic plate.

[0017] Compared with the prior art, the beneficial effects of the present utility model:

[0018] 1. When the device for studying the element transfer by arbuscular mycorrhizal fungal hyphae of the present utility model is in use, soil is filled in both planting chambers, and plants are planted in both planting chambers. Exemplarily, for example, corn is planted in one planting chamber, and Sedum alfredii Hance is planted in the other planting chamber. Arbuscular mycorrhizal fungi and heavy metals are applied in any one of the two planting chambers. As time goes by, the arbuscular mycorrhizal fungi grow hyphae and establish symbiosis with the roots of the plants in the planting chamber where the arbuscular mycorrhizal fungi are applied. The hyphae pass through the nylon net and enter the soil and plants in the other planting chamber, and establish symbiosis with the plants in the other planting chamber. The heavy metals are transmitted to the plants in the planting chamber without the application of heavy metals through the hyphae. In this way, the heavy metal content in the plants in the planting chamber without the application of heavy metals is all conducted by the hyphae. Thus, by calculating the heavy metal element content in the plants in the planting chamber without the application of heavy metals, the absolute value of the element transfer between the two plants by the hyphae can be studied, which facilitates the research of scientific researchers.

[0019] 2. The device for studying the element transfer by arbuscular mycorrhizal fungal hyphae of the present utility model integrates two planting chambers on the same planting box. The bottom plate of the planting box is the common bottom plate of the two planting chambers. Since the bottom plate of the planting box is a planar structure, the bottom plates of the two planting chambers are flush and there is no height difference. At the same time, since the isolation device separates the two planting chambers, and there is a water leakage part on the bottom plate of the planting box between the two partitions, the water in one planting chamber will not enter the other planting chamber, avoiding the entry of heavy metal elements in one planting chamber into the other planting chamber with water.

[0020] 3. The bottom plates of the two planting chambers are flush, so the bottom between the two partitions is a planar structure, that is, the distance between the two partitions is a straight line. Since there is a height difference in the bottom plates of the existing two planting chambers, the distance between the two partitions between the existing two planting chambers is an oblique line. It can be seen that the distance between the two partitions involved in the present utility model is shorter, which is beneficial to the miniaturization of the whole device.

[0021] 4. Since the two planting chambers are integrated on the same planting box and the bottom plate of the planting box is a planar structure, the bottom plate of the planting box is the common bottom plate of the two planting chambers, and there is no height difference between the bottom plates of the two planting chambers. In this way, the device of the present utility model can be placed on a horizontal plane for use without designing support legs. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic structural diagram of the device for studying the element transfer by arbuscular mycorrhizal fungal hyphae according to Embodiment 1;

[0023] Figure 2 It is a top view of the device for studying the element transfer by arbuscular mycorrhizal fungal hyphae according to Embodiment 1;

[0024] Figure 3 Figure 2 is a schematic structural view of another angle of the device for studying the hyphal transfer elements of arbuscular mycorrhizal fungi in Example 1;

[0025] Figure 4 Figure 3 is a schematic structural view of the partition in Example 1;

[0026] Figure 5 Figure 4 is a schematic structural view of the connection between the partition and the first nylon net in Example 1;

[0027] Figure 6 Figure 5 is a schematic structural view of the device for studying the hyphal transfer elements of arbuscular mycorrhizal fungi in Example 2.

[0028] Reference numerals in each drawing:

[0029] 1. Planting box; 10. Planting chamber; 11. Acrylic support; 2. Partition; 20. Hollowed-out part; 3. First nylon net; 4. Second nylon net; 5. Water leakage part; 6. Drainage hole; 7. Left planting chamber; 70. Middle planting chamber; 71. Right planting chamber. Detailed implementation manners

[0030] It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0031] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "width", "upper", "lower", "front", "rear", "top", "bottom", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0032] In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, the terms "installed", "connected", "connected", "set" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, a direct connection, or an indirect connection through an intermediate medium, and can be the internal communication of two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0033] In the present utility model, unless otherwise clearly defined and limited, the first feature being "above" or "below" the second feature may include direct contact between the first and second features, or may include the first and second features not being in direct contact but in contact through additional features therebetween. Moreover, the first feature being "above", "over" and "on top of" the second feature includes the first feature being directly above and obliquely above the second feature, or merely indicating that the horizontal height of the first feature is higher than that of the second feature. The first feature being "below", "beneath" and "underneath" the second feature includes the first feature being directly below and obliquely below the second feature, or merely indicating that the horizontal height of the first feature is less than that of the second feature.

[0034] Embodiment 1

[0035] Please refer to Figure 1 - Figure 5 The present utility model provides a device for studying the element transfer of arbuscular mycorrhizal fungi hyphae, including a planting box 1. The bottom plate of the planting box 1 is of a planar structure. At least one isolation device is arranged in the planting box 1. In this embodiment, there is one isolation device, and one isolation device forms two planting chambers 10 in the planting box 1; the isolation device includes two partition plates 2 arranged at a horizontal interval. The partition plates 2 are hermetically connected to the bottom plate of the planting box 1, and the partition plates 2 are also hermetically connected to the relatively arranged first side plate and second side plate of the planting box 1. A hollow portion 20 is formed in the middle of the partition plate 2, and a first nylon net 3 is covered on the hollow portion 20. The first nylon net 3 is used to block the penetration of plant roots and allow the penetration of hyphae. A water leakage portion 5 located between the two partition plates 2 is further opened on the bottom plate of the planting box 1, and the water leakage portion 5 penetrates through the opposite surfaces of the bottom plate of the planting box 1.

[0036] In summary, it can be understood that: 1. When the device for studying the element transfer of arbuscular mycorrhizal fungi hyphae of the present utility model is in use, soil is filled in both of the two planting chambers 10, and plants are planted in the two planting chambers 10. Exemplarily, for example, corn is planted in one planting chamber 10, and Sedum alfredii Hance is planted in the other planting chamber 10. Arbuscular mycorrhizal fungi and heavy metals are applied in one of the planting chambers 10. As time goes by, the arbuscular mycorrhizal fungi grow hyphae and establish a symbiosis with the roots of the plants in the planting chamber 10 where the arbuscular mycorrhizal fungi are applied. The hyphae penetrate through the nylon net and enter the soil and plants in the other planting chamber 10, and establish a symbiosis with the plants in the other planting chamber 10. The heavy metals are transmitted to the plants in the planting chamber 10 where no heavy metals are applied by means of the hyphae. In this way, the heavy metal content in the plants in the planting chamber 10 where no heavy metals are applied is all conducted by the hyphae. Thus, by calculating the heavy metal element content in the plants in the planting chamber 10 where no heavy metals are applied, the absolute value of the element transfer between the two plants by the hyphae can be studied, which facilitates the research of scientific researchers.

[0037] 2. The device for studying the hyphal transfer elements of arbuscular mycorrhizal fungi in the present utility model integrates two planting chambers 10 on the same planting box 1. The bottom plate of the planting box 1 is the common bottom plate of the two planting chambers 10. Since the bottom plate of the planting box 1 is a planar structure, the bottom plates of the two planting chambers 10 are flush and there is no height difference. At the same time, since the isolation device separates the two planting chambers 10, and there is a water leakage part 5 on the bottom plate of the planting box 1 located between the two partition plates 2, the water in one of the planting chambers 10 enters the space between the two partition plates 2 through the first nylon net 3 and then will be discharged outside the planting box 1 through the water leakage part 5 between the two partition plates 2. Therefore, the water in one of the planting chambers 10 will not enter the other planting chamber 10, avoiding the heavy metal elements in one planting chamber 10 from entering the other planting chamber 10 with water and affecting the experimental results.

[0038] 3. The bottom plates of the two planting chambers 10 are flush, so the bottom between the two partition plates 2 is a planar structure, that is, the distance between the two partition plates 2 is a straight line. Since the bottom plates of the existing two planting chambers 10 have a height difference structure, the distance between the two partition plates 2 between the existing two planting chambers 10 is an oblique line. It can be seen that the distance between the two partition plates 2 involved in the present utility model is shorter, which is beneficial to the miniaturization of the whole device.

[0039] 4. Since the two planting chambers 10 are integrated on the same planting box 1 and the bottom plate of the planting box 1 is a planar structure, the bottom plate of the planting box 1 is the common bottom plate of the two planting chambers 10, and there is no height difference between the bottom plates of the two planting chambers 10. Thus, it is not necessary to design support legs to place the device of the present utility model on a horizontal plane for use.

[0040] It should be noted that when the volume of the planting chamber 10 of the present utility model is the same as that of the planting chamber 10 in the existing patent CN217591615U, the volume of the device of the present utility model is significantly smaller.

[0041] Of course, in other embodiments, for example, in one planting chamber 10, corn is planted, and in another planting chamber 10, Sedum alfredii Hance is planted. In one of the planting chambers 10, arbuscular mycorrhizal fungi and plant nutrient elements such as nitrogen, phosphorus, potassium and other elements are applied. As time goes by, the arbuscular mycorrhizal fungi grow hyphae and establish symbiosis with the roots of the plants in the planting chamber 10 where the arbuscular mycorrhizal fungi are applied. The hyphae penetrate through the nylon net and enter the soil and plants in another planting chamber 10, and establish symbiosis with the plants in that another planting chamber 10. The nutrient elements are transmitted to the plants in the planting chamber 10 where no nutrient elements are applied through the hyphae. In this way, the nutrient element content in the plants in the planting chamber 10 where no nutrient elements are applied is all conducted by the hyphae. By calculating the nutrient element content in the plants in the planting chamber 10 where no nutrient elements are applied, the absolute value of the element transmission between the two plants by the hyphae can be studied, which facilitates the research of scientific researchers.

[0042] In this embodiment, a drainage part is arranged at the bottom in the planting chamber 10. The drainage part penetrates through the opposite surfaces of the bottom plate of the planting box 1. Specifically, the drainage part includes a plurality of drainage holes 6 arranged in an array, which is beneficial to the discharge of excessive water in the soil in the planting chamber 10. Among them, a second nylon net 4 is fixedly covered on the drainage part. The second nylon net 4 is used to block the penetration of the roots of the plants and allow the hyphae to penetrate. It can be understood from this that when there is too much water in the soil in the planting chamber 10, it will be discharged out of the planting chamber 10 through the drainage part, avoiding the roots of the plants from rotting due to excessive water; in addition, the hyphae in the planting chamber 10 can also penetrate through the second nylon net 4 and extend outside the planting chamber 10 to absorb the nutrients in the soil outside the planting chamber 10, which is beneficial to promoting the growth of the plants and will not affect the experimental results at the same time.

[0043] In this embodiment, the bottom between the two partition plates 2 is hollowed out to form the above-mentioned water leakage part 5, which can increase the water leakage area of the water leakage part 5 and is beneficial to quickly discharge the water between the two partition plates 2 out of the planting box 1. Of course, in other embodiments, a water leakage hole penetrating through the opposite surfaces of the bottom plate of the planting box 1 can also be opened at the bottom between the two partition plates 2, and the water leakage hole is configured as the water leakage part 5.

[0044] In this embodiment, the distance between the two partition plates 2 is set to L, and the relationship satisfied by the L is: 0.5≤L≤1.5 cm. The distance between the two partition plates 2 in this embodiment is 1.5 cm. Of course, in other embodiments, the distance between the two partition plates 2 can also be 0.5 cm or 0.8 cm.

[0045] In this embodiment, acrylic support seats 11 are provided at the four corners of the bottom plate of the planting box 1. During use, the acrylic support seats 11 usually come into contact with the soil. The acrylic support seats 11 can exhibit characteristics such as wear resistance, high temperature resistance, ultraviolet resistance, and corrosion resistance, which is beneficial to improving the lifespan of the entire device.

[0046] In this embodiment, the first side plate or the second side plate of the planting box 1 is a transparent acrylic plate, which is beneficial for observing the situation inside the planting box 1. It can be observed whether the roots of the plants in the planting chamber 10 will pass through the pores of the nylon net, so as to facilitate adjusting the mesh number of the nylon net. In this embodiment, the nylon net with a mesh number of 500 is selected, that is, the mesh numbers of the first nylon net 3 and the second nylon net 4 are both 500.

[0047] It should be noted that both the first nylon net 3 and the second nylon net 4 can be fixed to the planting box 1 by means of glue pasting; the partition can also be fixed inside the planting box 1 by means of gluing.

[0048] Embodiment Two

[0049] The difference between this embodiment and Embodiment One lies in: the number of partition devices is different. Referring to Figure 6 , two partition devices are provided in this embodiment, and the two partition devices form 3 planting chambers 10 inside the planting box 1. The 3 planting chambers 10 are the left planting chamber 7, the middle planting chamber 70, and the right planting chamber 71 respectively.

[0050] During use, soil is filled in the left planting chamber 7, the middle planting chamber 70, and the right planting chamber 71. Exemplarily, plants are planted in the middle planting chamber 70, and no plants are planted in the left planting chamber 7 and the right planting chamber 71. Heavy metals are applied in the left planting chamber 7 and the right planting chamber 71 without plants, and arbuscular mycorrhizal fungi are applied in the middle planting chamber 70 with plants. As time goes by, the arbuscular mycorrhizal fungi grow hyphae and establish a symbiosis with the roots of the plants in the planting chamber 10 where the arbuscular mycorrhizal fungi are applied. The hyphae penetrate through the nylon net and enter the soil in the left planting chamber 7 and / or the right planting chamber 71. The heavy metals in the left planting chamber 7 and / or the right planting chamber 71 will be transmitted to the plants in the middle planting chamber 70 through the hyphae. In this way, by calculating the heavy metal element content in the plants in the middle planting chamber 70, the absolute value of the element transmitted by the hyphae for a single plant can be studied, which facilitates the research of scientific researchers.

[0051] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. Any equivalent structure or equivalent process transformation made by using the description and drawings of the present invention, or directly or indirectly applied in other related technical fields, shall be included in the patent protection scope of the present invention by the same token.

Claims

1. An apparatus for studying the transfer of elements by arbuscular mycorrhizal fungal hyphae, characterized in that, It includes a planting box. The bottom plate of the planting box is a planar structure. At least one isolation device is arranged in the planting box. One isolation device forms two planting chambers in the planting box. The isolation device includes two partitions arranged horizontally at intervals. The partitions are hermetically connected to the bottom plate of the planting box, and the partitions are also hermetically connected to the relatively arranged first side plate and second side plate of the planting box. The middle of the partition is hollowed out, and the hollowed-out part is covered with a first nylon net. The first nylon net is used to block the penetration of plant roots and allow the penetration of hyphae. A water leakage part located between the two partitions is also opened on the bottom plate of the planting box, and the water leakage part penetrates the opposite surfaces of the bottom plate of the planting box.

2. The device for studying the transfer of elements by arbuscular mycorrhizal fungal hyphae according to claim 1, characterized in that, A drainage part is arranged at the bottom of the planting chamber. The drainage part penetrates the opposite surfaces of the bottom plate of the planting box, and a second nylon net is fixedly covered on the drainage part. The second nylon net is used to block the penetration of plant roots and allow the penetration of hyphae.

3. The device for studying the transfer of elements by arbuscular mycorrhizal fungal hyphae according to claim 2, wherein The drainage part includes a plurality of drainage holes arranged in an array.

4. The device for studying the transfer of elements by arbuscular mycorrhizal fungal hyphae according to claim 1, characterized in that, The bottom between the two partitions is hollowed out to form the water leakage part.

5. The device for studying the transfer of elements by arbuscular mycorrhizal fungal hyphae according to claim 1, characterized in that, Leakage holes penetrating the opposite surfaces of the bottom plate of the planting box are opened at the bottom between the two partitions, and the leakage holes are configured as the water leakage part.

6. The device for studying the transfer of elements by arbuscular mycorrhizal fungal hyphae according to claim 1, characterized in that, The distance between the two partitions is set as L, and the relationship satisfied by L is: 0.5 ≤ L ≤ 1.5 cm.

7. The device for studying the transfer of elements by arbuscular mycorrhizal fungal hyphae according to claim 1, characterized in that, Acrylic support seats are arranged at the four corner positions of the bottom plate of the planting box.

8. The device for studying the transfer of elements by arbuscular mycorrhizal fungal hyphae according to claim 1, characterized in that, The first side plate or the second side plate of the planting box is a transparent acrylic plate.

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