Flat plate agglutination detection device

Abstract

The utility model provides a flat plate agglutination detection device which comprises a movable platform, a detection platform is arranged on the movable platform, the detection platform comprises a base, the base is in a hollow cone shape, a light source is arranged in the base, a light reflecting layer is arranged on the inner surface of the base, and a detachable glass plate is arranged on the top of the base. The base is provided with a cover plate capable of covering the glass plate, the cover plate is movably connected with the base, and a plurality of detection holes are distributed in the cover plate. The plate agglutination detection device disclosed by the utility model can reduce water evaporation, can collect a plurality of samples in one detection glass plate at one time, realizes batch detection through integral shaking, reduces the operation time, can be used for plate agglutination detection of pullorum disease and the like, and has a good application prospect.

Description

Technical Field

[0001] The utility model relates to the field of poultry epidemic prevention, and particularly relates to a plate agglutination detection device. Background Art

[0002] Pullorum disease is an infectious disease caused by Salmonella pullorum, which is one of the important diseases endangering the chicken farming industry. In China, the infection of pullorum disease is relatively common, seriously restricting the development of the breeding industry of broilers and laying hens in China. Since pullorum disease can be transmitted vertically and horizontally, it is very difficult to completely eliminate it only through treatment or prevention in the infected chicken farms. Taking regular detection and eliminating positive individuals in the breeding chicken farms is the most effective measure to control and purify this disease at present.

[0003] With the emphasis on the purification state of pullorum disease in the medium- and long-term animal epidemic prevention and control plan by the state, and the establishment of a disease-free enterprise certification system, market access and information release system to guide the development of the purification work of breeding livestock and poultry diseases, the purification of pullorum disease has become a key task for breeding enterprises at present. Generally, the whole flock of breeding chickens is detected three times at the ages of 100 - 120, 200 - 230, and 300 - 310 days.

[0004] At present, the detection method for pullorum disease is the whole blood plate agglutination test. The commonly used equipment is a plate agglutination detection box, which consists of a table, a glass plate, an open box, and a light bulb. The light-emitting light bulb is placed in the box. The vein on the inner side of the wing of the chicken to be tested is punctured with a syringe needle, 30 μL of the flowing blood is aspirated, and after being fully mixed with the antigen, the glass plate needs to be shaken well, and the agglutination reaction is observed. The detection efficiency is relatively slow. Content of the Utility Model

[0005] The purpose of the utility model is to provide a plate agglutination detection device, which can improve the detection efficiency of plate agglutination and is used for plate agglutination detection such as pullorum disease.

[0006] To achieve the above purpose, the technical solution of the utility model is as follows:

[0007] A plate agglutination detection device includes a movable platform, and a detection platform is arranged on the movable platform. The detection platform includes a base, the base is in a hollow conical shape, a light source is arranged in the base, a reflective layer is arranged on the inner surface of the base, a detachable glass plate is arranged on the top of the base, the base is provided with a cover plate that can cover the glass plate, the cover plate is movably connected with the base, and a plurality of detection holes are distributed on the cover plate.

[0008] Further, a vibration motor is arranged on the base.

[0009] Further, the base is movably connected with the movable platform through an adjustable locking device.

[0010] Further, the adjustable locking device includes a ball head, which is connected to the base through a ball head connecting rod. The ball head is embedded in a ball seat, and the ball seat is connected to the movable platform through a ball seat connecting rod. The ball head can rotate within the ball seat. The bottom of the ball seat has a screw and a fastening nut connected to the ball seat connecting rod, and the fastening nut is used to adjust the movement of the ball head.

[0011] Further, there is at least one crank on the base. The function of the crank is that when the detection platform is movably connected to the movable platform in a manner of adjustable locking connection, the detection platform can be shaken through the crank.

[0012] Further, the light source is a cold light source. The advantage of using a cold light source is to prevent thermal radiation and avoid evaporation of the sample due to thermal radiation. The cold light source can be an LED light source.

[0013] Further, the movable platform includes a support frame. There are universal wheels at the bottom of the support frame, and there is at least one storage rack within the support frame.

[0014] Further, the bottom of the support frame has a bottom plate. There are two layers of storage racks above the bottom plate. The outer edge of the storage rack has a fence. There is an enclosed fence between the bottom plate and its upper storage rack. A power supply is provided on the bottom plate, and the power supply is electrically connected to the light source. There is a side door on the enclosed fence.

[0015] Further, the bottom of at least one storage rack has a movable flat plate that can be unfolded to one side of the movable platform.

[0016] Further, the cover plate is a transparent cover plate. The purpose of using a transparent cover plate is to provide a better view, facilitating observation and operation.

[0017] The beneficial effects of the present utility model are as follows:

[0018] 1. By using a movable platform, the individual to be detected can be detected nearby, reducing the collection distance, decreasing the probability of cross-infection during transportation, and also saving time costs;

[0019] 2. By using a conical base, the light source can be concentrated through the reflective layer. At the same time, the light source goes from bottom to top, penetrating the glass plate and the test sample, increasing the clarity of the detection and facilitating observation;

[0020] 3. Through the design of the detection platform, by shielding the detection glass plate, the loss of sample moisture is reduced. Therefore, multiple standard antigens can be added dropwise at one time, multiple samples can be collected on one detection glass plate, and the whole plate can be shaken to observe the coagulation reaction, thus realizing batch detection and reducing the operation time. Description of the Drawings

[0021] Figure 1 . It is a schematic structural diagram of the flat agglutination detection device of the present utility model;

[0022] Figure 2 . It is Figure 1 a schematic structural diagram of the adjustable locking device component in

[0023] Among them, 1. Movable platform, 2. Detection platform, 3. Adjustable locking device, 301. Ball head, 302. Ball seat, 303. Fastening nut, 304. Ball head connecting rod, 305. Ball seat connecting rod, 4. Base, 5. Cover plate, 6. Glass plate, 7. Light source, 8. Reflective layer, 9. Crank, 10. Hinge, 11. Lock, 12. Support frame, 13. Shelf, 14. Bottom plate, 15. Enclosure, 16. Side door, 17. Universal wheel, 18. Movable flat plate, 19. Power supply, 20. Vibration motor. Specific embodiments

[0024] Now, example embodiments will be described more fully with reference to the accompanying drawings. However, the example embodiments can be implemented in various forms and should not be construed as limited to the examples set forth herein; rather, these embodiments are provided so that this utility model will be more complete and comprehensive, and will fully convey the concept of the example embodiments to those skilled in the art. The described features, structures, or characteristics can be combined in any suitable manner in one or more embodiments. In the following description, numerous specific details are provided to give a thorough understanding of the embodiments of this utility model. However, those skilled in the art will realize that one or more of the specific details can be omitted when practicing the technical solutions of this utility model, or other methods, components, devices, steps, etc. can be adopted. In other cases, well-known technical solutions are not shown or described in detail to avoid obscuring the various aspects of this utility model.

[0025] As Figure 1 shown, in this example, it includes a movable platform 1, and a detection platform 2 is provided on the movable platform 1. The detection platform 2 includes a base 4. The base 4 is in a hollow conical shape. A light source 7 is provided inside the base 4. A reflective layer 8 is provided on the inner surface of the base 4. A detachable glass plate 6 is provided on the top of the base 4. The base 4 has a cover plate 5 that can cover the glass plate 6. The cover plate 5 is movably connected to the base 4, and a plurality of detection holes are distributed on the cover plate 5.

[0026] In this example, the detachable glass plate 6 is placed on the upper end of the base 4. The inner edge of the upper end of the base 4 is recessed, so that the glass plate 6 can be laid on the upper end of the base 4. Of course, a plurality of inwardly protruding fulcrums or support rods can also be provided inside the upper end of the base 4 to support the glass plate 6. Compared with the conventional detection glass plate, the glass plate 6 in this example can adopt a glass plate 6 with a larger area for detecting multiple samples at one time.

[0027] The cover plate 5 is used to cover the glass plate 6 to prevent the evaporation of the moisture of the sample dropped on the glass plate 6 and form a physical isolation between samples to prevent cross - contamination. The cover plate 5 can be connected to the base 4 through a hinge 10. On the side of the cover plate 5 opposite to the hinge 10, there is a lock 11 that can be buckled with the base 4. The opening and closing of the cover plate 5 can be realized through the hinge 10 and the lock 11. The detection holes on the cover plate 5 are used for spotting. For easy observation, the cover plate 5 can be transparent or at least partially transparent. The diameter of the detection holes on the cover plate 5 can be 1 - 5 cm, such as 2, 3, 4 cm, and the distance from the spotting hole to the glass plate 6 is 1 - 3 cm. In this example, the diameter of the detection holes is set to 4 cm, and about 100 detection holes can be set on the entire cover plate 5, so that 100 samples can be batch - tested before replacing the glass plate 6.

[0028] The base 4 is conical and has a reflective layer 8, which is beneficial to gather light on the glass plate 6. The light source 7 is preferably a cold light source, such as an LED lamp, a fluorescent lamp, etc. When using an LED lamp, a lens can also be set on the lamp beads.

[0029] In this example, a vibration motor 20 is provided on the base 4. Through the vibration motor 20, the mixing operation of the antigen - antibody of the test sample can be carried out instead of the traditional method of shaking the glass plate 6.

[0030] The detection platform 2 is fixed on the movable platform 1 through its base 4. In this example, the base 4 and the movable platform 1 are movably connected through an adjustable locking device 3. Specifically, the ball head 301 is connected to the base 4 through a ball - head connecting rod 303. The ball head 301 is embedded in the ball socket 302. The ball socket 302 is connected to the movable platform 1 through a ball - socket connecting rod 305. The ball head 301 can rotate in the ball socket 302. The bottom of the ball socket 302 has a fastening nut 303 screwed on the ball - socket connecting rod 305, and the fastening nut 303 is used to adjust the movement of the ball head 301. The ball - socket connecting rod 305 is hollow at least at one end of the ball socket 4, and a connecting rod connected to the fastening nut 303 is provided inside it. The other end of the connecting rod is connected to a sheet - shaped fastening hemisphere (such as a hemispherical steel sheet), and the fastening hemisphere is arranged between the ball socket 302 and the ball head 301. The place where the ball - socket connecting rod 305 is connected to the fastening nut 303 has a thread. When the fastening nut 303 is tightened, the fastening nut 303 pushes the fastening hemisphere to lock the ball head 301, and vice versa to loosen the ball head 301.

[0031] A level is also provided on the base 4, and the detection platform 2 can be leveled through the above - mentioned adjustable locking device 3.

[0032] One embodiment of the present utility model is to install at least one crank 9 on the base 4. The function of the crank 9 is to shake the detection platform 2 when the detection platform 2 is movably connected to the movable platform 1 in a manner of adjustable locking and movable connection through the crank 9.

[0033] The movable platform 1 includes a support frame 12, and universal wheels 17 are provided at the bottom of the support frame 12 for moving the device. The universal wheels 17 are provided with locking devices for locking the rotation of the universal wheels 17. In this way, the detection device can be conveniently moved and maintained stable during detection.

[0034] The movable platform 1 can be rectangular or triangular, and the corresponding support frame 12 is also rectangular or triangular. In this example, it is rectangular. A storage rack 13 is further provided inside the support frame 12. According to needs, the storage rack 13 can be set to one layer, two layers or three layers. The support frame 12 is also provided with hooks, and hooks can also be provided at the edge or bottom of the storage rack 13. The outer edge of the storage rack 13 has a retaining wall 15. Blood collection-related tools and detection reagents, such as alcohol lamps, sterilization meshes, spreading iron rings, pipettes, syringes, disinfection bottles, antibodies, etc. can be placed on the storage rack. The sterilization mesh is erected on the alcohol lamp through a bracket, and the outer flame of the alcohol lamp just lies on the sterilization mesh. The outer circle of the sterilization mesh has a groove for assuming the spreading iron ring, so that when sterilizing the spreading iron ring, it can be directly placed on the sterilization mesh for sterilization. A groove matching the bottom of the alcohol lamp is also provided on the storage rack, so that the bottom position of the alcohol lamp can be embedded in the groove to avoid the sliding of the alcohol lamp.

[0035] A bottom plate 14 is provided at the bottom of the support frame 12, and there is a closed retaining wall between the bottom plate 14 and the upper storage rack 13. A power supply 19 is provided on the bottom plate 14, and the power supply 19 is electrically connected to the light source 7. A side door 16 is provided on the closed retaining wall. Since the space on this layer of the bottom plate 14 is mainly for placing the power supply 19, a closed method is adopted.

[0036] In addition, a movable flat plate 18 that can be unfolded to one side of the movable platform 1 is further provided at the bottom of the storage rack 13. Specifically, for example, slide rails are provided at the bottom of the storage rack 13, and sliding blocks are provided on both sides of the movable flat plate 18 to be embedded in the slide rails, and a limiting device is provided on the slide rails so that the movable flat plate 18 stops unfolding after unfolding to a certain width. In this way, the movable flat plate 18 can be unfolded and will not slide off. Other methods can also be used to movably connect the movable flat plate 18 to the bottom of the storage rack 13. In addition, a flexible gasket / bushing or other vibration isolation methods can be added between the detection platform 2 and the movable platform 1 to reduce the vibration transmission between the detection platform 2 and the movable platform 1. Of course, appropriately loosening the adjustable locking device 3 can also play a certain role in vibration isolation.

[0037] The flat agglutination detection device of the utility model is mainly used for the flat agglutination detection of pullorum disease in chicken coops. Of course, it can also be used in other places where flat agglutination detection is required. When in use, first disinfect the flat agglutination detection device, open the cover plate 5, load the clean glass plate 6 into the detection platform 2, cover the cover plate 5 and fasten it. Load other items, such as blood collection related tools, detection reagents, etc., into the storage rack 13. Open the locking device of the universal wheel 17 and push the detection device to the place where it is needed, such as beside a specific cage position. The detection device can move and follow the arrangement direction of chickens for detection. Lock the locking device of the universal wheel 17 and level the detection platform 2. Open the movable plate 18 to serve as an operating table. Before the worker catches the chicken, drop the pullorum antigen on the glass plate 6 in the detection hole. In order to improve the detection efficiency, 10 antigen holes are dropped at one time. Then the worker starts to catch the chicken and place it on the movable plate 18. The blood collection personnel collect the blood, and the blood of each chicken is evenly smeared in the detection hole and fully mixed with the antigen. The detection hole has a certain depth to prevent the smear on the front from drying out when the plate is shaken together after detecting 10 chickens at a time, which is not conducive to observing the results. Each used smear iron ring is placed on the sterilization net and sterilized by the flame of an alcohol lamp, saving the workload of one person. By adjusting the adjustable locking device 3 of the base 4, appropriately adjust the locking force to achieve the balance between the rotation force and the detection rack. Shake the detection platform 2 with the crank 9 to achieve the purpose of mixing antigen and antibody, without having to pick up the glass plate 6 for shaking. After detecting 100 samples, open the cover plate 5 to replace the glass plate 6, clean and disinfect the used one, and repeat for large-scale continuous detection. After use, clean all the utensils, perform sterilization treatment, and charge for the next use.

Claims

1. A plate agglutination detection device, characterized in that, It includes a movable platform, on which there is a detection platform. The detection platform includes a base, the base is in a hollow conical shape, there is a light source inside the base, there is a reflective layer on the inner surface of the base, there is a detachable glass plate on the top of the base, the base has a cover plate that can cover the glass plate, the cover plate is movably connected to the base, and there are several detection holes distributed on the cover plate.

2. The detection device according to claim 1, wherein There is a vibration motor on the base.

3. The detection device according to claim 1, characterized in that, The base is movably connected to the movable platform through an adjustable locking device.

4. The detection device according to claim 3, wherein The adjustable locking device includes a ball head, the ball head is connected to the base through a ball head connecting rod, the ball head is embedded in a ball seat, the ball seat is connected to the movable platform through a ball seat connecting rod, the ball head can rotate in the ball seat, there are screws at the bottom of the ball seat and fastening nuts connected to the ball seat connecting rod, and the fastening nuts are used to adjust the movement of the ball head.

5. The detection device according to claim 4, characterized in that, There is at least one crank on the base.

6. The detection device according to claim 1, characterized in that, The light source is a cold light source.

7. The detection device according to claim 1, wherein, The movable platform includes a support frame, there are universal wheels at the bottom of the support frame, and there is at least one storage rack inside the support frame.

8. The detection device according to claim 7, characterized in that, There is a bottom plate at the bottom of the support frame, there are two layers of storage racks above the bottom plate, there are enclosures at the outer edges of the storage racks, there is a closed enclosure between the bottom plate and its upper storage rack, there is a power supply arranged on the bottom plate, the power supply is electrically connected to the light source, and there is a side door on the closed enclosure.

9. The detection device according to claim 8, characterized in that, The bottom of at least one layer of storage rack has a movable flat plate that can be unfolded to one side of the movable platform.

10. The detection device according to claim 1, characterized in that, The cover plate is a transparent cover plate.

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