Portable PCR (Polymerase Chain Reaction) eight-connection sleeve

Abstract

The utility model discloses a portable PCR (Polymerase Chain Reaction) eight-connection sleeve, belongs to the technical field of polymerase chain reaction (PCR) eight-connection tubes, and solves the problem that the existing PCR eight-connection tube is inconvenient to use. The device specifically comprises a casing pipe box, and the casing pipe box is internally divided into eight casing pipe fixing cavities through seven partition plates; a group of sleeve fixing clamps are arranged in each sleeve fixing cavity; the sleeve fixing clamp comprises a first clamping arm and a second clamping arm, one end of the first clamping arm is hinged to one end of the second clamping arm through a hinge shaft, and the other end of the first clamping arm and the other end of the second clamping arm are connected with a first control panel and a second control panel respectively. The eight second control plates are movably connected with an integral control rod assembly penetrating through the eight sleeve fixing cavities; the eight first control panels are movably connected with the eight independent control panel assemblies respectively. According to the kit disclosed by the utility model, when PCR detection is carried out and an experimenter independently takes one reagent tube, the fixing effect on other reagent tubes cannot be relieved, so that the reagent tubes are flexible to take and convenient to operate, and the influence caused by other reagent tubes is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of polymerase chain reaction (PCR) eight-tube couplings, in particular to a portable PCR eight-tube coupling. Background Art

[0002] Polymerase chain reaction (PCR) is a method of enzymatically synthesizing specific DNA fragments in vitro. It is carried out through cycles of high-temperature denaturation, low-temperature annealing (renaturation), and thermophilic extension, so that the target DNA can be rapidly amplified. The standard PCR experimental site is divided into a reagent preparation area, a specimen preparation area, an amplification area, and a product analysis area. Based on anti-contamination requirements, PCR reaction reagents are often prepared in the reagent preparation area and packaged in PCR eight-tubes. Sample nucleic acid is extracted in the specimen preparation area. After the nucleic acid extraction is completed, the PCR eight-tube containing the reaction solution is opened and the extracted nucleic acid sample is added. This partitioning operation can separate the PCR reagent preparation from the nucleic acid product preparation, which can effectively prevent the contamination of the reagents by the nucleic acid extraction product.

[0003] When using the eight-tube strip, the PCR eight-tube strip needs to be placed on the mounting seat, and then the reagents are poured into the tube body to facilitate the reaction. However, the current PCR eight-tube strip usually includes eight reagent tubes and an eight-tube strip sleeve. The eight reagent tubes are fixed side by side in the eight-tube strip sleeve to form a whole. When used, it is placed on the mounting seat; however, when placed on the mounting seat, the two cannot be fixed, so the stability of the reagent tube is poor and it is easy to tilt and shake.

[0004] To solve the above problems, a Chinese utility model patent with authorization publication number CN215050226U discloses an anti-contamination PCR eight-tube strip. In this patent, the reagent tubes are fixed by two curved plates to prevent them from tipping over. However, the curved plates in the eight circular holes are all operated by a lever handle, that is, the eight reagent tubes are fixed by the two curved plates at the same time and are also released at the same time. When taking out or putting in one of the reagent tubes, the lever handle must be operated to release the fixation of all the reagent tubes. In PCR testing, the experimenter needs to take the reagent tubes one by one and repeatedly perform operations such as centrifugation and ice water bath. Therefore, the eight-tube strip provided by the above patent is not flexible enough to use and is not convenient to operate. Moreover, when taking out one of the reagent tubes, the lever handle must be operated to release the fixation of all the reagent tubes, which is likely to affect other reagent tubes. Utility Model Content

[0005] In view of the deficiencies in the prior art, the utility model provides a portable PCR eight-tube sleeve, which solves the problem that the existing PCR eight-tube sleeve is inconvenient to use.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0007] A portable PCR eight-tube cannula includes a cannula box, wherein the cannula box is divided into eight cannula fixing cavities by seven partitions; each cannula fixing cavity is provided with a set of cannula fixing clips, and each set of cannula fixing clips holds a reagent tube;

[0008] The casing fixing clamp includes a first clamp arm and a second clamp arm, one end of the first clamp arm and the second clamp arm are hinged by a hinge shaft, and the hinge shaft is movably connected to the casing fixing cavity; the other ends of the first clamp arm and the second clamp arm intersect, and are respectively connected to the first control plate and the second control plate after the intersection;

[0009] The eight second control panels are all movably connected to an integral control rod assembly that passes through the eight sleeve fixing cavities; the eight first control panels are respectively movably connected to the eight separate control panel assemblies.

[0010] In this solution, eight second control panels can be controlled simultaneously through the overall control rod assembly, and the eight second control panels respectively drive the eight second clamping arms to move, so as to adjust the distance between the first clamping arm and the second clamping arm, so as to facilitate the simultaneous placement or removal of eight reagent tubes; one of the first control panels can be controlled individually through the individual control panel assembly, and the first control panel drives the corresponding first clamping arm to move, so as to adjust the distance between the first clamping arm and the second clamping arm, so as to facilitate the placement or removal of a certain reagent tube; when conducting PCR testing, the experimenter needs to take the reagent tubes one by one, and repeatedly perform operations such as centrifugation and ice water bath. The design of the overall control rod assembly and the individual control panel assembly does not release the fixing effect on other reagent tubes when taking a certain reagent tube alone, so the taking is flexible and the operation is convenient, avoiding the influence of other reagent tubes.

[0011] Furthermore, circular holes are provided on both ends of the casing box and on the seven partitions;

[0012] The overall control rod assembly includes an opening and closing rod, which passes through the circular holes on the casing box and the partition; the eight second control plates are rotatably connected to the opening and closing rod through eight rotating shafts.

[0013] In this solution, when taking or placing eight reagent tubes at the same time, the opening and closing rod can be pushed. The opening and closing rod drives the second clamping arm to move toward the first clamping arm through the second control panel to clamp the reagent tubes between the two; the work efficiency is high and the operation is convenient.

[0014] Furthermore, two pressing plates are respectively connected to both ends of the opening and closing rod.

[0015] In this solution, the pressing plate increases the end area of the opening and closing rod, making operation easier; at the same time, it can prevent the opening and closing rod from sliding out along the circular hole.

[0016] Furthermore, the separate control panel assembly includes an opening and closing plate, which is rotatably connected to a strip hole opened in the side wall of the casing box through a rotating shaft; one end of the opening and closing plate abuts the first control panel, and the other end of the opening and closing plate extends outside the casing box.

[0017] In this solution, when taking or placing one of the reagent tubes separately, the opening and closing plate can be turned to rotate. The opening and closing plate drives the first clamping arm to move toward the second clamping arm through the first control plate to clamp the reagent tube between the two, which is flexible to operate.

[0018] Furthermore, a side of the first control board away from the second control board, and a side of the second control board away from the first control board are both connected to a limit block.

[0019] In this solution, the limit block limits the first clamp arm and the second clamp arm to prevent the second clamp arm from retreating under the drive of the first clamp arm when the first clamp arm moves toward the second clamp arm, thereby causing the reagent tube to be loosely clamped; and to prevent the first clamp arm from retreating under the drive of the second clamp arm when the second clamp arm moves toward the first clamp arm, thereby causing the reagent tube to be loosely clamped.

[0020] Furthermore, a compression spring is provided between the first control plate and the second control plate.

[0021] In this solution, under the elastic force of the compression spring, the first clamping arm and the second clamping arm can reset themselves to ensure that the reagent tube is clamped and fixed.

[0022] Furthermore, a circular hole is provided on the top of the sleeve fixing cavity; the upper end of the reagent tube is accommodated in the circular hole, and the lower end of the reagent tube is clamped in the sleeve fixing clamp.

[0023] Furthermore, a box cover is hinged on the top of the casing box.

[0024] In this solution, a box cover is provided to prevent the samples in the reagent tubes from being contaminated.

[0025] The beneficial effects of the utility model are:

[0026] When the portable PCR eight-tube sleeve provided by the utility model is in use, the eight second clamping arms can be driven to move simultaneously by the opening and closing rod, and one of the first clamping arms can be driven to move individually by the opening and closing plate; the two operation modes correspond to the operation when taking eight reagent tubes and the operation when taking one reagent tube, respectively, and the operation is flexible, avoiding affecting other reagent tubes when taking one reagent tube.

[0027] During PCR testing, when the experimenter takes a certain reagent tube for centrifugation, ice water bath and other operations, he only needs to operate the opening and closing plate corresponding to the reagent tube to release the fixing effect on the reagent tube without affecting the fixing effect on other reagent tubes. It is easy to operate and suitable for the use scenario of taking reagent tubes one by one during PCR testing. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the structure of a portable PCR eight-tube sleeve of the utility model;

[0029] Figure 2 This is a schematic diagram of the horizontal cross-section structure of a portable eight-tube PCR system of the present invention;

[0030] Figure 3 This is a schematic diagram of the vertical cross-section structure of a portable PCR eight-tube sleeve of the utility model;

[0031] Figure 4 This is a structural diagram of the casing fixing clamp of the utility model.

[0032] Reference numerals:

[0033] 1. Casing box; 2. Partition; 3. Casing fixing clamp; 31. First clamp arm; 32. Second clamp arm; 33. Articulated shaft; 41. First control panel; 42. Second control panel; 43. Compression spring; 5. Overall control lever assembly; 51. Opening and closing lever; 52. Pressing plate; 6. Individual control panel assembly; 61. Opening and closing plate; 7. Reagent tube; 8. Stop block; 9. Box cover; DETAILED DESCRIPTION

[0034] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. The specific embodiments of the present invention are described below to facilitate understanding of the present invention by those skilled in the art. However, it should be understood that the present invention is not limited to the specific embodiments. For those skilled in the art, as long as various variations are within the spirit and scope of the present invention as defined and determined by the appended claims, these variations are obvious, and all inventions and creations utilizing the concepts of the present invention are protected.

[0035] like Figure 1-Figure 3 As shown, this embodiment provides a portable PCR eight-tube cannula, which corresponds to two operation modes when taking eight reagent tubes or taking one reagent tube, respectively, making it convenient to take one reagent tube separately; it specifically includes:

[0036] Casing box 1, partition 2 and casing fixing clamp 3;

[0037] The casing box 1 is divided into eight casing fixing chambers by seven partitions 2; each casing fixing chamber is provided with a set of casing fixing clamps 3, and each set of casing fixing clamps 3 holds a reagent tube 7.

[0038] like Figure 4As shown, the cannula fixing clamp 3 includes a first clamp arm 31 and a second clamp arm 32. One end of the first clamp arm 31 and the second clamp arm 32 are hinged by a hinge shaft 33, and the hinge shaft 33 is movably connected to the cannula fixing cavity; the other ends of the first clamp arm 31 and the second clamp arm 32 intersect, and after crossing, they are respectively connected to the first control board 41 and the second control board 42; the eight second control boards 42 are each movably connected to an integral control rod assembly 5 that runs through the eight cannula fixing cavities; the eight first control boards 41 are respectively movably connected to eight individual control board assemblies 6. When performing PCR testing, the experimenter needs to take the reagent tubes 7 one by one and repeatedly perform operations such as centrifugation and ice water bath. The design of the integral control rod assembly 5 and the individual control board assembly 6 ensures that when a single reagent tube 7 is taken, the fixing effect on other reagent tubes 7 will not be released, making the taking flexible and easy to operate.

[0039] Circular holes are provided at both ends of the casing box 1 and on the seven partitions 2; the integral control rod assembly 5 includes an opening and closing rod 51, which passes through the circular holes on the casing box 1 and the partitions 2; the eight second control plates 42 are rotatably connected to the opening and closing rod 51 via eight rotating shafts; when eight reagent tubes 7 are taken or placed at the same time, the opening and closing rod 51 can be pushed, and the opening and closing rod 51 drives the second clamping arm 32 to move toward the first clamping arm 31 through the second control plate 42 to clamp the reagent tubes 7 between the two; the work efficiency is high and the operation is convenient.

[0040] Two pressing plates 52 are connected to both ends of the opening and closing rod 51 respectively; the pressing plates 52 increase the end area of the opening and closing rod 51 to facilitate operation; and at the same time prevent the opening and closing rod 51 from sliding out along the circular hole.

[0041] In this embodiment, the opening and closing rod 51 drives the second control plate 42 to move through the rotating shaft, and the movement of the second control plate 42 is a small arc; a strip groove can be set on the first control plate 41 at the position for connecting the rotating shaft, and when the second control plate 42 moves, the rotating shaft moves back and forth in the strip groove.

[0042] The individual control plate assembly 6 includes an opening and closing plate 61, which is pivotally connected to a slot in the side wall of the cannula box 1 via a rotating shaft. One end of the opening and closing plate 61 abuts the first control plate 41, while the other end extends outside the cannula box 1. To remove or place a single reagent tube 7, the opening and closing plate 61 is rotated. The opening and closing plate 61, through the first control plate 41, drives the first clamping arm 31 toward the second clamping arm 32, thereby clamping the reagent tube 7 between them.

[0043] A side of the first control plate 41 away from the second control plate 42 , and a side of the second control plate 42 away from the first control plate 41 are both connected to the limit block 8 .

[0044] The limit block 8 limits the first clamping arm 31 and the second clamping arm 32 to prevent the second clamping arm 32 from retreating under the drive of the first clamping arm 31 when the first clamping arm 31 moves toward the second clamping arm 32, thereby loosening the clamping of the reagent tube 7; and to prevent the first clamping arm 31 from retreating under the drive of the second clamping arm 32 when the second clamping arm 32 moves toward the first clamping arm 31, thereby loosening the clamping of the reagent tube 7.

[0045] A compression spring 43 is provided between the first control plate 41 and the second control plate 42. Under the elastic force of the compression spring 43, the first clamping arm 31 and the second clamping arm 32 can automatically reset, ensuring that the reagent tube 7 is clamped and secured. A circular hole is provided at the top of the cannula fixing cavity; the upper end of the reagent tube 7 is accommodated in the circular hole, and the lower end of the reagent tube 7 is clamped in the cannula fixing clamp 3.

[0046] A box cover 9 is hinged on the top of the sleeve box 1 to prevent the sample in the reagent tube 7 from being contaminated.

[0047] The working principle of this embodiment is:

[0048] When the portable eight-tube PCR cartridge provided in this embodiment is used, when taking out eight reagent tubes 7, the opening and closing lever 51 is manipulated, and the opening and closing lever 51 simultaneously drives the eight second control plates 42 to move, and the eight second control plates 42 respectively drive the eight second clamping arms 32 to move, so as to adjust the distance between the first clamping arm 31 and the second clamping arm 32, thereby facilitating the simultaneous placement or removal of eight reagent tubes 7. When taking out one of the reagent tubes 7, the opening and closing plate 61 corresponding to the reagent tube 7 is manipulated, and the opening and closing plate 61 drives the first control plate 41 to move, and the first control plate 41 drives the first clamping arm 31 connected thereto to move, thereby adjusting the distance between the first clamping arm 31 and the second clamping arm 32, thereby facilitating the placement or removal of the reagent tube 7 individually.

[0049] Those skilled in the art will appreciate that the embodiments herein are intended to help readers understand the principles of the present invention, and should be understood that the scope of protection of the present invention is not limited to such specific descriptions and embodiments. Those skilled in the art can, based on the technical teachings disclosed in this utility model, make various other specific variations and combinations that do not depart from the essence of the present invention, and such variations and combinations are still within the scope of protection of the utility model.

Claims

1. A portable PCR eight-tube set, characterized by: The invention comprises a cannula box (1), wherein the cannula box (1) is divided into eight cannula fixing cavities by seven partitions (2); a set of cannula fixing clamps (3) is provided in each cannula fixing cavity, and a reagent tube (7) is clamped in each set of cannula fixing clamps (3); The sleeve fixing clamp (3) comprises a first clamp arm (31) and a second clamp arm (32), one end of the first clamp arm (31) and the second clamp arm (32) are hinged via a hinge shaft (33), and the hinge shaft (33) is movably connected to the sleeve fixing cavity; the other ends of the first clamp arm (31) and the second clamp arm (32) intersect with each other, and after the intersection, they are respectively connected to a first control plate (41) and a second control plate (42); The eight second control plates (42) are all movably connected to an integral control rod assembly (5) that passes through the eight sleeve fixing cavities; and the eight first control plates (41) are respectively movably connected to eight separate control plate assemblies (6).

2. The portable eight-tube PCR system according to claim 1, characterized in that: Circular holes are provided at both ends of the casing box (1) and on the seven partitions (2); The integral control rod assembly (5) comprises an opening and closing rod (51), which is arranged to pass through the circular holes on the casing box (1) and the partition (2); and the eight second control plates (42) are rotatably connected to the opening and closing rod (51) via eight rotating shafts.

3. The portable eight-tube PCR system according to claim 2, characterized in that: Two pressing plates (52) are respectively connected to the two ends of the opening and closing rod (51).

4. The portable eight-tube PCR system according to claim 3, characterized in that: The separate control panel assembly (6) comprises an opening and closing plate (61), which is rotatably connected to a strip hole provided on a side wall of the casing box (1) via a rotating shaft; one end of the opening and closing plate (61) abuts against the first control panel (41), and the other end of the opening and closing plate (61) extends outside the casing box (1).

5. The portable eight-tube PCR system according to claim 4, characterized in that: A side of the first control plate (41) away from the second control plate (42), and a side of the second control plate (42) away from the first control plate (41) are both connected to a limiting block (8).

6. The portable eight-tube PCR system according to claim 1, characterized in that: A compression spring (43) is provided between the first control plate (41) and the second control plate (42).

7. The portable eight-tube PCR system according to claim 1, characterized in that: A circular hole is provided at the top of the sleeve fixing cavity; the upper end of the reagent tube (7) is accommodated in the circular hole, and the lower end of the reagent tube (7) is clamped in the sleeve fixing clamp (3).

8. The portable eight-tube PCR system according to claim 1, characterized in that: The top of the casing box (1) is also hinged with a box cover (9).

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