Constant-temperature nucleic acid amplification analyzer

Abstract

The utility model provides a constant-temperature nucleic acid amplification analyzer which comprises a lower shell, an upper cover and a base, the lower shell and the upper cover are connected together through a hinge, the base is fixed to the bottom of the lower shell, and the lower shell, the upper cover and the base are connected to form a containing cavity. The heating component is connected to the bearing body through a heat-conducting medium; and the detection module comprises a circular motion mechanism and a signal acquisition module. The constant-temperature nucleic acid amplification analyzer disclosed by the utility model solves the problems that the existing equipment is low in integration level, large in size, high in cost and inconvenient to carry, and has the advantages of small size, low cost, high integration, convenience in carrying and no limitation of use scenes.

Description

Technical Field

[0001] The utility model relates to the field of nucleic acid detection, in particular to a constant temperature nucleic acid amplification analyzer. Background Art

[0002] Molecular diagnosis is a relatively important branch in the IVD field. With the market's demand for testing, the proportion of molecular diagnostic tests has increased rapidly. Due to its unique high specificity and very high detection sensitivity, molecular diagnosis is the gold standard in the IVD testing field. Molecular diagnostic technology has played a very important role in infectious respiratory diseases, sexually transmitted diseases, HPV and other diseases. Nucleic acid amplification reaction technology is used in detection to detect signals from the amplified template. Nucleic acid amplification technology generally uses variable temperature amplification technology and constant temperature amplification technology. Variable temperature amplification generally uses a fluorescent PCR instrument, while constant temperature amplification only requires providing a constant temperature for the reaction sample.

[0003] PCR technology requires a PCR instrument, which cycles between high and low temperatures during the reaction. This results in long detection times, high power consumption, high cost, and bulky size, making it inconvenient to use in certain scenarios or situations. PCR amplification efficiency is far lower than that of constant-temperature amplification technology. Generally, constant-temperature amplification efficiency is 10 to 100 times higher than that of PCR. However, current constant-temperature nucleic acid amplification analyzers on the market are generally not highly integrated, are relatively large, and are expensive, making them difficult to miniaturize and portable. Utility Model Content

[0004] The main purpose of the utility model is to provide a constant temperature nucleic acid amplification analyzer, which overcomes the above technical problems.

[0005] In order to achieve the above purpose, the present invention proposes the following technical solutions:

[0006] A constant temperature nucleic acid amplification analyzer, comprising:

[0007] The lower shell, upper cover, and base are connected together by a hinge. The base is fixed to the bottom of the lower shell. The three are connected to form a receiving cavity, in which are installed:

[0008] The temperature module includes a main control board and a carrier. The main control board is provided with a heating component, and the heating component is connected to the carrier via a heat conducting medium.

[0009] The detection module includes a circular motion mechanism and a signal acquisition module.

[0010] The circular motion mechanism includes: a motor mounting base, a stepper motor, a coupling, and a slip ring connector. The stepper motor is installed at the lower end of the motor mounting base. The output shaft of the stepper motor passes through the upper end of the motor mounting base and is sleeved with the lower end shaft hole of the coupling. The slip ring is sleeved with the upper end shaft hole of the coupling. One end of the connector is sleeved on the outer diameter of the coupling. The main control board is connected to the carrier at the top.

[0011] Furthermore, the signal acquisition module is fixed to one side of the connector and is provided with a left shell and a right shell, which cooperate with each other to form an accommodating space, in which are installed: an LED light board to provide excitation light for detection; a first filter is provided on the side close to the bottom of the LED light board, a first plano-convex lens is provided on the side of the first filter away from the LED light board, a dichroic mirror is provided on the side of the first plano-convex lens away from the first filter, a second plano-convex lens is provided below the dichroic mirror, a third plano-convex lens is provided above the dichroic mirror, a second filter is provided above the third plano-convex lens, and a receiving board assembly is provided above the second filter.

[0012] Furthermore, a battery is provided on the base, and the battery is fixed to the base by an adhesive device.

[0013] Furthermore, a rubber pad is provided on the bottom surface of the base.

[0014] Furthermore, the motor mounting base is provided with side holes for routing the wiring harness of the motor wires.

[0015] Furthermore, two fixing columns are provided on the side of the motor mounting base for mounting a power board.

[0016] Furthermore, the motor mounting seat is also provided with a spring pin hole for the spring pin to pass through and connect to the battery.

[0017] Furthermore, a power drive circuit is provided on the power board.

[0018] Furthermore, a Type-C interface is provided on the power board.

[0019] Furthermore, the lower shell is provided with a key switch connected to a compression spring.

[0020] The constant temperature nucleic acid amplification analyzer of the present invention solves the problems of low integration, large size, high cost and non-portability of existing equipment. It has the advantages of small size, low cost, high integration, easy to carry and not restricted by usage scenarios. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The drawings constituting part of this application are provided to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are provided to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0022] Figure 1 This is a three-dimensional assembly diagram of a constant temperature nucleic acid amplification analyzer of the present utility model.

[0023] Figure 2 This is a three-dimensional assembly diagram of the signal acquisition module.

[0024] Figure 3 This is a structural diagram of the motor mounting base.

[0025] Figure 4 Schematic diagram of the connector structure.

[0026] Figure 5 Schematic diagram of the structure of the carrier.

[0027] Figure 6 This is a cross-sectional view of the entire constant temperature nucleic acid amplification analyzer of the present utility model.

[0028] Figure 7 This is the overall appearance of a constant temperature nucleic acid amplification analyzer of the present utility model.

[0029] The above drawings include the following reference numerals:

[0030] 01. Lower shell; 02. Upper cover; 03. Base; 04. Rubber pad; 05. Hinge; 06. Motor mount; 07. Stepper motor; 08. Coupling; 09. Slip ring; 10. Connector; 11. Signal acquisition module; 12. Main control board; 13. Carrier; 14. Power board; 15. Battery; 16. Adhesive device; 17. Switch button; 18. Compression spring; 1101. Right shell; 1102. Left shell; 1103. LED light board; 1104. First filter; 1105. First plano-convex lens; 1106. Second plano-convex lens; 1107. Dichroic mirror; 1108. Third plano-convex lens; 1109. Second filter; 1110. Receiving board assembly. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is in no way intended to limit the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0032] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0033] Unless otherwise specifically stated, the relative arrangement of the parts and steps, the numerical expressions and the numerical values set forth in these embodiments do not limit the scope of the present invention. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the drawings are not drawn according to the actual proportional relationship. The techniques, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, the techniques, methods and equipment should be considered as part of the authorization specification. In all examples shown and discussed here, any specific values should be interpreted as being merely exemplary and not as limitations. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following figures, and therefore, once an item is defined in one figure, it does not need to be further discussed in subsequent figures.

[0034] Reference below Figures 1 to 7 , the utility model is further described:

[0035] A constant temperature nucleic acid amplification analyzer, comprising:

[0036] The lower shell 01, the upper cover 02, and the base 03 are connected together by a hinge 05. The base 03 is fixed to the bottom of the lower shell 01. The three are connected to form a receiving cavity, in which are installed:

[0037] The temperature module includes a main control board 12 and a carrier 13. The main control board 12 is provided with a heating component, and the heating component is connected to the carrier 13 via a heat conducting medium;

[0038] The carrier 13 is made of metal with good thermal conductivity, generally aluminum alloy. A tapered hole for loading the sample tube is processed on the carrier, and the bottom of the tapered hole is transparent. The main control board 12 is also provided with a circular hole corresponding to the tapered transparent hole on the carrier 13. The main control board 12 is also provided with a mounting hole corresponding to the carrier 13. In addition, a heating component is provided on the main control board 12, such as a heating resistor or a heating wire, to heat the carrier 13. A heat-conducting material is filled between the heating component and the carrier 13. The heat-conducting medium can be a heat-conducting silicone pad, heat-conducting glue, heat-conducting cotton, etc.

[0039] The detection module includes a circular motion mechanism and a signal acquisition module 11.

[0040] The circular motion mechanism includes: a motor mounting base 06, a stepper motor 07, a coupling 08, a slip ring 09, and a connector 10. The stepper motor 07 is mounted on the lower end of the motor mounting base 06. The output shaft of the stepper motor 07 passes through the upper end of the motor mounting base 06 and is sleeved with the lower end shaft hole of the coupling 08. The slip ring 09 is sleeved with the upper end shaft hole of the coupling 08. One end of the connector 10 is sleeved on the outer diameter of the coupling 08. The main control board 12 is connected to the carrier 13 above.

[0041] There are two through holes on the side of coupling 08, which are used for routing the slip ring harness. The motor output shaft is sleeved into the lower end shaft hole of coupling 08, and the slip ring 09 is inserted into the upper end shaft hole of coupling 08. Then the connector 10 is sleeved on the outer diameter of coupling 08. There are fixing holes on the connector 10 corresponding to the fastening holes on the side of coupling 08, and there are also wiring harness routing holes corresponding to the side of coupling 08. Two bolts are used on the connector 10 to clamp and fix it through the fixing holes.

[0042] In this embodiment, two fixing columns are further provided on the side of the motor mounting base 06 for mounting the power board 14 .

[0043] The power board 14 is provided with a Type-C charging interface, and is also provided with a motor drive circuit for connecting to the main control board 12 for power supply.

[0044] In a preferred embodiment, a through hole is provided on the bottom surface of the motor mounting seat 06, which corresponds to the fixing hole of the main control board 12 fixing the carrier 13, so as to facilitate the passage of tools through the through hole to fasten the main control board 12 and the carrier 13.

[0045] In this embodiment, the motor mounting base 06 is provided with side holes for routing the wiring harness of the motor wires.

[0046] In this embodiment, the motor mounting seat 06 is further provided with a spring pin hole for the spring pin to pass through and contact the output contact of the battery 15 to provide power.

[0047] In this embodiment, a power drive circuit is provided on the power board 14 to supply power to various components of the nucleic acid amplification analyzer.

[0048] In this embodiment, a Type-C interface is provided on the power board 14 , and the battery 15 can be charged by connecting to an external power source via the Type-C interface.

[0049] In this embodiment, the lower shell 01 is provided with a button switch 17, which is connected to a compression spring 18. When in use, the switch button 17 is pressed to open the upper cover 02, and the test sample is placed in the conical hole of the carrier, and then the upper cover 02 is buckled to perform detection and analysis.

[0050] In this embodiment, the signal acquisition module 11 is fixed to one side of the connector 10, and is provided with a left shell 1102 and a right shell 1101, which cooperate with each other to form an accommodating space, in which are installed: an LED light board 1103 to provide excitation light for detection; a first filter 1104 is provided on the side close to the bottom of the LED light board 1103, a first plano-convex lens 1105 is provided on the side of the first filter 1104 away from the LED light board 1103, a dichroic mirror 1107 is provided on the side of the first plano-convex lens 1105 away from the first filter 1104, a second plano-convex lens 1106 is provided below the dichroic mirror 1107, a third plano-convex lens 1108 is provided above the dichroic mirror 1107, a second filter 1109 is provided above the third plano-convex lens 1108, and a receiving board assembly 1110 is provided above the second filter 1109.

[0051] The right shell 1101 and the left shell 1102 serve as fixed carriers and packaging for internal parts; the LED light board 1103 provides excitation light for detection; the first filter 1104 filters out unnecessary bands of the excitation light, making the band of the excitation light narrower and purer, and the plano-convex lens corrects the optical path; the dichroic mirror 1107 reflects the excitation light downward and transmits the fluorescence upward; the second filter 1109 filters out stray light and reduces background noise; the receiving board assembly 1124 receives the fluorescence signal.

[0052] The signal acquisition module 11 is fixed on the side hole of the connector 10. The wiring harness of the signal acquisition module 11 is connected to the cables output from both sides of the connector in the slip ring. The wiring harness at the other end of the slip ring 09 is connected to the wiring harness interface of the main control board 12. The diameter formed by the end hole of the second plano-convex lens 1106 of the signal acquisition module 11 performing circular motion in the entire assembly is equal to the diameter of the circle formed by the sample loading conical hole in the carrier 13. The stepper motor 07 can drive the excitation light spot emitted by the signal acquisition module 11 to pass through the bottom of the sample tube to excite the fluorescent substance of the sample and detect the fluorescent signal emitted by the sample during the uniform circular motion.

[0053] In this embodiment, the bonding device 16 uses double-sided tape, and the battery 15 is bonded to the base 03 through the double-sided tape.

[0054] In this embodiment, a rubber pad 04 is provided on the bottom surface of the base 03 .

[0055] In the description of the present invention, it needs to be understood that the directions or positional relationships indicated by directional words such as "front, back, up, down, left, right", "horizontal, vertical, vertical, horizontal" and "top, bottom" are usually based on the directions or positional relationships shown in the drawings. They are only for the convenience of describing the present invention and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the device or element referred to must have a specific direction or be constructed and operated in a specific direction. Therefore, they cannot be understood as limiting the scope of protection of the present invention; the directional words "inside and outside" refer to the inside and outside relative to the outline of each component itself.

[0056] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A constant temperature nucleic acid amplification analyzer, characterized in that: include: A lower shell (01), an upper cover (02), and a base (03), wherein the lower shell (01) and the upper cover (02) are connected together via a hinge (05), and the base (03) is fixed to the bottom of the lower shell (01). The three are connected to form a receiving cavity, in which the following components are installed: A temperature module comprises a main control board (12) and a carrier (13), wherein a heating element is provided on the main control board (12), and the heating element is connected to the carrier (13) via a heat-conducting medium; A detection module, comprising a circular motion mechanism and a signal acquisition module (11); The circular motion mechanism comprises: a motor mounting seat (06), a stepper motor (07), a coupling (08), a slip ring (09), and a connector (10). The stepper motor (07) is mounted on the lower end of the motor mounting seat (06). The output shaft of the stepper motor (07) passes through the upper end of the motor mounting seat (06) and is sleeved with the lower end shaft hole of the coupling (08). The slip ring (09) is sleeved with the upper end shaft hole of the coupling (08). One end of the connector (10) is sleeved on the outer diameter of the coupling (08). The upper end of the main control board (12) is connected to the carrier (13).

2. A constant temperature nucleic acid amplification analyzer according to claim 1, characterized in that: The signal acquisition module (11) is fixed to one side of the connector (10), and is provided with a left shell (1102) and a right shell (1101), which cooperate with each other to form a receiving space, wherein the receiving space is provided with: an LED light board (1103) for providing excitation light for detection; a first filter (1104) is provided on a side close to the bottom of the LED light board (1103); a first plano-convex lens (1104) is provided on a side of the first filter (1104) away from the LED light board (1103); 05), a dichroic mirror (1107) is provided on the side of the first plano-convex lens (1105) away from the first filter (1104), a second plano-convex lens (1106) is provided below the dichroic mirror (1107), a third plano-convex lens (1108) is provided above the dichroic mirror (1107), a second filter (1109) is provided above the third plano-convex lens (1108), and a receiving plate assembly (1110) is provided above the second filter (1109).

3. A constant temperature nucleic acid amplification analyzer according to claim 1, characterized in that: A battery (15) is provided on the base (03), and the battery (15) is fixed on the base (03) via an adhesive device (16).

4. A constant temperature nucleic acid amplification analyzer according to claim 1, characterized in that: The bottom surface of the base (03) is provided with a rubber pad (04).

5. The constant temperature nucleic acid amplification analyzer according to claim 1, characterized in that: The motor mounting seat (06) is provided with side holes for routing the wiring harness of the motor wires.

6. The constant temperature nucleic acid amplification analyzer according to claim 1, characterized in that: Two fixing columns are also provided on the side of the motor mounting seat (06) for mounting a power supply board (14).

7. The constant temperature nucleic acid amplification analyzer according to claim 3, characterized in that: The motor mounting seat (06) is also provided with a spring pin hole for the spring pin to pass through and connect to the battery (15).

8. The constant temperature nucleic acid amplification analyzer according to claim 6, characterized in that: The power supply board (14) is provided with a power supply driving circuit.

9. The constant temperature nucleic acid amplification analyzer according to claim 6, characterized in that: The power board (14) is provided with a Type-C interface.

10. The constant temperature nucleic acid amplification analyzer according to claim 1, characterized in that: The lower housing (01) is provided with a key switch (17), which is connected to a compression spring (18).

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