Vesicle puncturing assembly for micro-fluidic chip and micro-fluidic biological analyzer

Abstract

The utility model discloses a vesicle puncturing assembly for a micro-fluidic chip and a micro-fluidic biological analyzer, and relates to the technical field of microfluidics, the vesicle puncturing assembly comprises a fixed frame 1, a movable frame 2, an air bag 3 and a puncturing head 4; the fixed frame 1 and the movable frame 2 are movably connected through a vertical sliding rail 5; the air bag 3 is fixedly mounted between the fixed frame 1 and the movable frame 2, and the air bag 3 drives the movable frame 2 to move up and down through bulging and shrinking; and the puncturing head 4 is fixedly mounted at the bottom of the movable frame 2 and is used for puncturing vesicles of the micro-fluidic chip 7 placed on the gas circuit board 6 when the movable frame 2 moves downwards. By adopting the vesicle puncturing assembly provided by the embodiment of the invention, the pressure can be output more stably, the control precision of the puncturing force is improved, and the vesicle puncturing assembly is more suitable for being used by various automatic micro-fluidic biological analyzers.

Description

Technical Field

[0001] The present application relates to the field of microfluidics technology, and in particular to a vesicle puncture component for a microfluidic chip and a microfluidic bioanalyzer. Background Art

[0002] Microfluidics integrates the fundamental operational elements of biological, chemical, and medical analysis, such as sample preparation, reaction, separation, and detection, onto a micron-scale chip, automating the entire analytical process. Microfluidics is currently a hot area in the development of micro-total analysis systems.

[0003] Microfluidic chip analysis uses chips as the operating platform, is based on analytical chemistry, relies on microelectromechanical processing technology, and features microchannel networks. With life sciences as its primary application, it is a key development focus in the field of micro-total analysis systems. Its goal is to integrate the entire laboratory function, including sampling, dilution, reagent addition, reaction, separation, and detection, onto a microchip that can be used multiple times. During the production process of microfluidic chips, a variety of reagents, such as lysis buffer, washing buffer, and eluent, must be pre-embedded in the chip.

[0004] Microfluidic chips contain vesicles. In practical applications, these vesicles need to be punctured to trigger the flow of liquid within the chip, thereby completing a series of operations. Currently, various microfluidic bioanalyzers have puncture heads that can be driven by motors or cylinders to move and puncture vesicles placed on the microfluidic chip on the gas manifold.

[0005] In the existing technology, the method of using a motor or cylinder to drive the puncture head to move cannot achieve precise control of the puncture force. If the force is too light, the vesicle cannot be punctured, and if the force is too strong, the microfluidic chip may be damaged, resulting in poor vesicle puncture effect. Utility Model Content

[0006] The embodiments of the present application provide a vesicle puncture component for a microfluidic chip and a microfluidic bioanalyzer to solve the problem of poor control accuracy of the puncture force of the vesicles in the microfluidic chip in the prior art.

[0007] The present invention provides a vesicle puncture component for a microfluidic chip, comprising:

[0008] Fixed frame 1, movable frame 2, air bag 3 and puncture head 4;

[0009] The fixed frame 1 and the movable frame 2 are movably connected via a vertical slide rail 5;

[0010] The airbag 3 is fixedly installed between the fixed frame 1 and the movable frame 2. The airbag 3 drives the movable frame 2 to move up and down by expanding and contracting.

[0011] The puncturing head 4 is fixedly mounted on the bottom of the movable frame 2 and is used to puncture the vesicles of the microfluidic chip 7 placed on the gas path plate 6 when the movable frame 2 moves downward.

[0012] Furthermore, the airbag 3 is fixedly installed between the fixed frame 1 and the movable frame 2 and is located below the fixed frame 1. It drives the movable frame 2 to move downward by inflating and drives the movable frame 2 to move upward by contracting.

[0013] Furthermore, the movable frame 2 includes an upper frame 21 and a lower frame 22, and the upper frame 21 and the lower frame 22 are connected by a column 23;

[0014] The fixed frame 1 is located between the upper frame 21 and the lower frame 22;

[0015] The airbag 3 is fixedly installed between the upper frame 21 and the fixed frame 1 and is located above the fixed frame 1. It drives the movable frame 2 to move upward by inflating and drives the movable frame 2 to move downward by contracting.

[0016] Furthermore, the airbag 3 includes an upper airbag 31 and a lower airbag 32;

[0017] The movable frame 2 includes an upper frame 21 and a lower frame 22, and the upper frame 21 and the lower frame 22 are connected by a column 23;

[0018] The fixed frame 1 is located between the upper frame 21 and the lower frame 22;

[0019] The upper airbag 31 is fixedly installed between the upper frame 21 and the fixed frame 1 and is located above the fixed frame 1. The lower airbag 32 is fixedly installed between the fixed frame 1 and the lower frame 22 and is located below the fixed frame 1.

[0020] The upper airbag 31 is expanded and the lower airbag 32 is contracted, thereby driving the movable frame 2 to move upward. The upper airbag 31 is contracted and the lower airbag 32 is expanded, thereby driving the movable frame 2 to move downward.

[0021] Furthermore, it also includes: a plurality of limit screws 8;

[0022] The plurality of limit screws 8 pass through the fixing frame 1 , are fixedly mounted on the lower frame 22 , and are located around the upper airbag 31 and the lower airbag 32 .

[0023] Furthermore, it also includes: a buffer pressure plate 9 and a compression spring 10;

[0024] The buffer pressure plate 9 is connected to the bottom of the movable frame 2 in a floating manner through the compression spring 10;

[0025] When the movable frame 2 moves downward, it drives the buffer pressing plate 9 to move downward and presses the microfluidic chip 7;

[0026] The movable frame 2 continues to move downward, driving the puncturing head 4 to puncture the vesicle, and at this time the compression spring 10 is compressed.

[0027] The present application also provides a microfluidic bioanalyzer, comprising:

[0028] Any of the above vesicle puncture components for a microfluidic chip.

[0029] The beneficial effects of this application include:

[0030] The vesicle puncturing assembly for a microfluidic chip provided in an embodiment of the present application includes a fixed frame 1, a movable frame 2, an airbag 3, and a puncturing head 4. The fixed frame 1 and the movable frame 2 are movably connected via a vertical slide rail 5. The airbag 3 is fixedly mounted between the fixed frame 1 and the movable frame 2, and drives the movable frame 2 up and down by expanding and contracting. The puncturing head 4 is fixedly mounted at the bottom of the movable frame 2 and is used to puncture the vesicles of the microfluidic chip 7 placed on the air path plate 6 when the movable frame 2 moves downward. Compared with motor-driven and cylinder-driven methods, driving by the expansion and contraction of the airbag can achieve a more stable output pressure, improve the control accuracy of the puncturing force, and is more suitable for use in various automated microfluidic bioanalyzers.

[0031] Other features and advantages of the present application will be described in the following description, and in part will become apparent from the description, or will be understood by practicing the present application. The purposes and other advantages of the present application can be realized and obtained by the structures particularly pointed out in the written description, claims, and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] The accompanying drawings are used to provide a further understanding of the present application and constitute a part of the specification. Together with the embodiments of the present application, they are used to explain the present application and do not constitute a limitation of the present application. In the accompanying drawings:

[0033] Figure 1A schematic diagram of the front structure of a vesicle puncture component for a microfluidic chip provided in an embodiment of the present application;

[0034] Figure 2 A schematic diagram of the rear structure of a vesicle puncture component for a microfluidic chip provided in an embodiment of the present application;

[0035] Figure 3 A schematic diagram of the rear structure of the puncture head of the vesicle puncture assembly for a microfluidic chip provided in an embodiment of the present application after puncturing the microfluidic chip;

[0036] Figure 4 Schematic diagram of the rear three-dimensional structure of the vesicle puncture component for the microfluidic chip provided in an embodiment of the present application. DETAILED DESCRIPTION

[0037] To provide an implementation scheme for improving the control accuracy of puncturing vesicles in a microfluidic chip, the present application provides a vesicle puncturing assembly for a microfluidic chip and a microfluidic bioanalyzer. Preferred embodiments of the present application are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are intended only to illustrate and explain the present application and are not intended to limit the present application. Furthermore, the embodiments and features of the embodiments may be combined with each other unless there is a conflict.

[0038] The present invention provides a vesicle puncture component for a microfluidic chip. Figure 1-4 Shown, including:

[0039] Fixed frame 1, movable frame 2, air bag 3 and puncture head 4;

[0040] The fixed frame 1 and the movable frame 2 are movably connected via a vertical slide rail 5;

[0041] The airbag 3 is fixedly installed between the fixed frame 1 and the movable frame 2. The airbag 3 drives the movable frame 2 to move up and down by expanding and contracting.

[0042] The puncturing head 4 is fixedly mounted on the bottom of the movable frame 2 and is used to puncture the vesicles of the microfluidic chip 7 placed on the gas path plate 6 when the movable frame 2 moves downward.

[0043] The above-mentioned vesicle puncture component provided in the embodiment of the present application is driven by the inflation and contraction of the airbag, compared with the motor-driven and cylinder-driven methods, which can output pressure more smoothly, improve the control accuracy of the puncture force, and is more suitable for use in various automated microfluidic bioanalyzers.

[0044] In one embodiment of the present application, the airbag 3 can be fixedly installed between the fixed frame 1 and the movable frame 2 and located below the fixed frame 1. The airbag 3 drives the movable frame 2 downward by inflating and drives the movable frame 2 upward by contracting.

[0045] For example, the airbag 3 is fixedly mounted on the fixed frame 1 and the movable frame 2 , and pushes the movable frame 2 downward when inflated, and pulls the movable frame 2 upward when deflated.

[0046] In one embodiment of the present application, the movable frame 2 may include an upper frame 21 and a lower frame 22 , and the upper frame 21 and the lower frame 22 are connected by a column 23 ;

[0047] The fixed frame 1 is located between the upper frame 21 and the lower frame 22, and the column 23 can pass through the fixed frame 1;

[0048] The airbag 3 can be fixedly installed between the upper frame 21 and the fixed frame 1, and is located above the fixed frame 1. It drives the movable frame 2 to move upward by inflating, and drives the movable frame 2 to move downward by contracting.

[0049] For example, the airbag 3 is fixedly mounted on the upper frame 21 and the fixed frame 1 , and pushes the upper frame 21 to move upward when inflated, and pulls the upper frame 21 to move downward when deflated.

[0050] In one embodiment of the present application, Figure 1-4 As shown, the airbag 3 may include an upper airbag 31 and a lower airbag 32;

[0051] The movable frame 2 may include an upper frame 21 and a lower frame 22 , and the upper frame 21 and the lower frame 22 are connected by a column 23 ;

[0052] The fixed frame 1 is located between the upper frame 21 and the lower frame 22, and the column 23 can pass through the fixed frame 1;

[0053] The upper airbag 31 is fixedly installed between the upper frame 21 and the fixed frame 1 and is located above the fixed frame 1. The lower airbag 32 is fixedly installed between the fixed frame 1 and the lower frame 22 and is located below the fixed frame 1.

[0054] The upper airbag 31 is expanded and the lower airbag 32 is contracted, driving the movable frame 2 to move upward, and the upper airbag 31 is contracted and the lower airbag 32 is expanded, driving the movable frame 2 to move downward;

[0055] For example, the upper airbag 31 is fixedly mounted on the fixed frame 1, and the lower airbag 32 is fixedly mounted on the lower frame 22. The upper airbag 31 inflates to push the upper frame 21 to move upward. At the same time, the lower airbag 32 contracts to reduce the space occupied between the lower frame 22 and the fixed frame 1, so that the lower frame 22 moves upward at the same time as the upper frame 21 moves upward; the lower airbag inflates to push the lower frame 22 to move downward. At the same time, the upper airbag 31 contracts to reduce the space occupied between the upper frame 21 and the fixed frame 1, so that the upper frame 21 moves downward at the same time as the lower frame 22 moves downward.

[0056] In the embodiment of this application, Figure 1-4 As shown, it may also include: a plurality of limiting screws 8;

[0057] A plurality of limit screws 8 pass through the fixing frame 1 and are fixedly mounted on the lower frame 22 , and are located around the upper airbag 31 and the lower airbag 32 .

[0058] The plurality of limit screws 8 can limit the horizontal diffusion of the upper airbag 31 and the lower airbag 32 during the expansion and contraction process, thereby concentrating the diffusion in the vertical direction.

[0059] Furthermore, the plurality of limit screws 8 may be polished screws, such as Figure 3 As shown, when the upper airbag 31 contracts and the lower airbag 32 bulges, pushing the movable frame 2 to move downward, the upper half of the limit screw 8 can pass through the fixed frame 1.

[0060] In an embodiment of the present application, the microfluidic chip can be placed on the air circuit board manually or by other automated devices. After the microfluidic chip is placed on the air circuit board, the clamping mechanism can be manually triggered to press the microfluidic chip on the air circuit board, and then the expansion and contraction of the airbag 3 can be controlled to drive the movable frame 2 to move, and further drive the puncture head 4 to puncture the vesicle of the microfluidic chip.

[0061] In one embodiment of the present application, automatic compaction of the microfluidic chip can also be achieved, such as Figure 1-4 As shown, the vesicle puncturing assembly may further include: a buffer pressure plate 9 and a compression spring 10;

[0062] The buffer pressure plate 9 is connected to the bottom of the movable frame 2 in a floating manner through a compression spring 10, that is, the distance between the buffer pressure plate 9 and the movable frame 2 can be changed by being compressed by the compression spring;

[0063] During the downward movement of the movable frame 2, the buffer pressing plate 9 is driven to move downward and press the microfluidic chip 7. After pressing the microfluidic chip 7, the buffer pressing plate 9 is limited by the microfluidic chip 7 and cannot move downward any further.

[0064] At this time, the movable frame 2 continues to move downward, which can drive the puncturing head 4 to puncture the vesicle, and the compression spring 10 is compressed.

[0065] After the inspection of the microfluidic chip 7 is completed, the airbag 3 controls the movable frame 2 to move upward, and at the same time drives the buffer pressure plate 9 to move upward, no longer pressing the microfluidic chip 7, so that the microfluidic chip 7 is reset and automatically sent out.

[0066] like Figure 1-4 As shown, the puncture head 4 can pass through the middle area of the buffer pressure plate 9, and the compression spring 10 can include multiple ones and is arranged around the buffer pressure plate.

[0067] The present application also provides a microfluidic bioanalyzer, comprising:

[0068] Any of the above vesicle puncture components for a microfluidic chip.

[0069] In the embodiments of the present application, the microfluidic bioanalyzer can be any known instrument for performing bioanalysis on a microfluidic chip, for example, a nucleic acid amplification fluorescence detection analyzer.

[0070] In summary, the vesicle puncture component for a microfluidic chip provided in the embodiment of the present application is driven by the inflation and contraction of the airbag, compared with motor-driven and cylinder-driven methods, and can output pressure more smoothly, improve the control accuracy of the puncture force, and is more suitable for use in various automated microfluidic bioanalyzers.

[0071] When an upper airbag and a lower airbag are included, the upper and lower airbags can cooperate with each other, one inflates and the other contracts, thereby achieving more flexible and effective lifting control of the movable frame, thereby driving the up and down movement of the puncture head.

[0072] The puncture head can be fixed on the bottom plate of the movable frame by screws, and the buffer pressure plate can be floatingly connected to the bottom plate of the movable frame through a compression spring, so that when pressing down, the microfluidic chip is first pressed tightly against the gas path plate and then punctured, so that the liquid in the vesicle will not leak, avoiding contamination of the sample and improving the reliability of sample detection.

[0073] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply the existence of any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or device comprising the element.

[0074] Obviously, those skilled in the art may make various changes and modifications to this application without departing from the spirit and scope of this application. Thus, if these modifications and variations of this application fall within the scope of the claims of this application and their equivalents, this application is intended to include these modifications and variations.

Claims

1. A vesicle puncture component for a microfluidic chip, characterized in that: include: A fixed frame (1), a movable frame (2), an air bag (3) and a puncture head (4); The fixed frame (1) and the movable frame (2) are movably connected via a vertical slide rail (5); The airbag (3) is fixedly installed between the fixed frame (1) and the movable frame (2), and the airbag (3) drives the movable frame (2) to move up and down by expanding and contracting; The puncturing head (4) is fixedly mounted on the bottom of the movable frame (2) and is used to puncture the vesicles of the microfluidic chip (7) placed on the air path plate (6) when the movable frame (2) moves downward.

2. The vesicle puncturing assembly according to claim 1, wherein: The airbag (3) is fixedly installed between the fixed frame (1) and the movable frame (2) and is located below the fixed frame (1). It drives the movable frame (2) to move downward by swelling and drives the movable frame (2) to move upward by shrinking.

3. The vesicle puncturing assembly according to claim 1, wherein: The movable frame (2) comprises an upper frame (21) and a lower frame (22), wherein the upper frame (21) and the lower frame (22) are connected via a column (23); The fixed frame (1) is located between the upper frame (21) and the lower frame (22); The airbag (3) is fixedly installed between the upper frame (21) and the fixed frame (1), and is located above the fixed frame (1). It drives the movable frame (2) to move upward by swelling, and drives the movable frame (2) to move downward by shrinking.

4. The vesicle puncturing assembly according to claim 1, wherein: The airbag (3) includes an upper airbag (31) and a lower airbag (32); The movable frame (2) comprises an upper frame (21) and a lower frame (22), wherein the upper frame (21) and the lower frame (22) are connected via a column (23); The fixed frame (1) is located between the upper frame (21) and the lower frame (22); The upper airbag (31) is fixedly installed between the upper frame (21) and the fixed frame (1), and is located above the fixed frame (1); the lower airbag (32) is fixedly installed between the fixed frame (1) and the lower frame (22), and is located below the fixed frame (1); The upper airbag (31) is expanded and the lower airbag (32) is contracted, thereby driving the movable frame (2) to move upward, and the upper airbag (31) is contracted and the lower airbag (32) is expanded, thereby driving the movable frame (2) to move downward.

5. The vesicle puncturing assembly according to claim 4, characterized in that Also includes: a plurality of limit screws (8); The plurality of limit screws (8) pass through the fixed frame (1), are fixedly mounted on the lower frame (22), and are located around the upper airbag (31) and the lower airbag (32).

6. The vesicle puncturing assembly according to any one of claims 1 to 5, characterized in that: Also includes: Buffering pressure plate (9) and compression spring (10); The buffer pressure plate (9) is floatingly connected to the bottom of the movable frame (2) via the compression spring (10); When the movable frame (2) moves downward, it drives the buffer pressure plate (9) to move downward and presses the microfluidic chip (7); The movable frame (2) continues to move downward, driving the puncturing head (4) to puncture the vesicle, and at this time the compression spring (10) is compressed.

7. A microfluidic bioanalyzer, characterized in that: include: The vesicle puncture component for a microfluidic chip according to any one of claims 1 to 6.

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