Gas replacement device for cell culture

Abstract

The utility model discloses a gas replacement device for cell culture, which comprises a box body, a sealing assembly arranged on the top of the box body, a gas outlet assembly arranged on the left side of the box body, a gas inlet pipe fixedly mounted on the right side of the box body, a gas inlet control valve arranged on the gas inlet pipe, and a mounting rack fixedly mounted on the surface of the gas inlet pipe, a sealing groove is formed in the right end of the air inlet pipe, a sealing ring is inserted into the sealing groove, a connecting pipe is fixedly mounted on the right side of the sealing ring, a mounting ring is fixedly mounted on the surface of the connecting pipe, a limiting groove is formed in the right side of the mounting ring, and a gas pressure reducing valve is arranged on the connecting pipe; and a gas tank is arranged at the bottom of the connecting pipe, and a limiting assembly is arranged on the mounting frame, so that mounting of the connecting pipe can be completed more quickly, a bolt mode for fixing is avoided, and the gas tank can be replaced more conveniently in the follow-up process.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a gas replacement device for cell culture. Background Art

[0002] Cell culture, also known as cell cloning technology, is formally referred to as cell culture technology in biology. Whether it's bioengineering technology as a whole or biological cloning technology as a specific area, cell culture is an essential process. Cell culture itself is the large-scale cloning of cells. Cell culture technology can transform a single cell into a simple single cell or minimally differentiated multicellular organisms through large-scale cultivation. This is an essential step in cloning technology, and cell culture itself is a form of cell cloning. Producing large numbers of cells or their metabolites through cell culture requires ventilation of the culture space during cell culture.

[0003] In the existing technology, the connecting pipe on the gas tank filled with gas is often connected to the air inlet pipe on the culture device so that the culture device can be ventilated. However, the connecting pipe on the existing gas tank and the air inlet pipe are generally connected by bolts, which makes the gas tank installation more troublesome and makes it difficult to replace the gas tank later. Therefore, the utility model provides a gas replacement device for cell culture. Utility Model Content

[0004] In view of the deficiencies in the prior art, the present invention provides a gas replacement device for cell culture, which is used to solve the problems raised in the above background technology.

[0005] The above technical objectives of the present utility model are achieved through the following technical solutions: A gas replacement device for cell culture, comprising a box body, a base fixedly installed on the inner wall of the box body, a culture plate overlapped on the base, a sealing assembly provided on the top of the box body, an air outlet assembly provided on the left side of the box body, an air inlet pipe fixedly installed on the right side of the box body, an air inlet control valve provided on the air inlet pipe, a mounting bracket fixedly installed on the surface of the air inlet pipe, and the mounting bracket is fixedly connected to the box body, a sealing groove is provided at the right end of the air inlet pipe, a sealing ring is inserted in the sealing groove, a connecting pipe is fixedly installed on the right side of the sealing ring, a mounting ring is fixedly installed on the surface of the connecting pipe, a limiting groove is provided on the right side of the mounting ring, a gas pressure reducing valve is provided on the connecting pipe, a gas tank is provided at the bottom of the connecting pipe, and a limiting assembly is provided on the mounting bracket.

[0006] Preferably, the sealing assembly includes a box cover hingedly arranged on the top of the box body, and a handle is fixedly installed on the top of the box cover.

[0007] Preferably, the air outlet assembly includes an air outlet pipe fixedly arranged on the left side of the box body, and an air outlet control valve is provided on the air outlet pipe.

[0008] Preferably, the limiting assembly includes a fixing frame fixedly arranged on the right side of the mounting frame, a driving motor is fixedly installed on the right side of the fixing frame, an output end of the driving motor passes through the fixing frame and extends to the inside of the fixing frame and is fixedly installed with a screw, a moving block is threadedly connected to the surface of the screw, the moving block is slidably connected to the fixing frame, and a first limiting block is fixedly installed on the left side of the moving block.

[0009] Preferably, the number of the fixing frames is two, and the two fixing frames are symmetrically distributed.

[0010] Preferably, the sealing ring is in a circular ring structure and is made of rubber.

[0011] Compared with the prior art, the beneficial effects of the present invention are: inserting the sealing ring on the left side of the connecting pipe into the sealing groove until the connecting pipe fits with the air inlet pipe, starting the drive motor, rotating the screw, causing the moving block to move to the left along the fixed frame, so that the first limit block is inserted into the limit groove until the moving block tightly squeezes the mounting ring, so that the installation of the connecting pipe can be completed more quickly, avoiding the use of bolts for fixing, and facilitating more convenient replacement of the gas tank later. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0013] Figure 2 It is a front cross-sectional view of the utility model;

[0014] Figure 3 It is an exploded view of the local structure of the utility model;

[0015] Figure 4 This is a schematic structural diagram of the position limiting assembly in Example 1 of the present utility model;

[0016] Figure 5 This is a schematic diagram of the structure of the limiting component in Example 2 of the present utility model.

[0017] In the figure, 1. box body; 2. base; 3. culture plate; 4. box cover; 5. handle; 6. air outlet pipe; 7. air outlet control valve; 8. air inlet pipe; 9. air inlet control valve; 10. mounting bracket; 11. sealing groove; 12. sealing ring; 13. connecting pipe; 14. mounting ring; 15. limit groove; 16. gas pressure reducing valve; 17. gas tank; 18. fixing bracket; 19. driving motor; 20. screw; 21. moving block; 22. first limit block; 23. supporting bracket; 24. fixing plate; 25. electric push rod; 26. moving plate; 27. second limit block; 28. slider; 29. slide rail. DETAILED DESCRIPTION

[0018] The present invention will be described in further detail below with reference to the accompanying drawings.

[0019] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by patent law.

[0020] Example 1:

[0021] Reference Figure 1-4A gas replacement device for cell culture is characterized in that it includes a box body 1, a base 2 is fixedly installed on the inner wall of the box body 1, a culture plate 3 is overlapped on the base 2, a sealing component is provided on the top of the box body 1, the sealing component includes a box cover 4 hingedly arranged on the top of the box body 1, and a handle 5 is fixedly installed on the top of the box cover 4 to facilitate sealing the top of the box body 1, an air outlet component is provided on the left side of the box body 1, the air outlet component includes an air outlet pipe 6 fixedly arranged on the left side of the box body 1, and an air outlet control valve 7 is provided on the air outlet pipe 6 to facilitate exhaust treatment during the ventilation process, and an air inlet pipe 8 is fixedly installed on the right side of the box body 1. An intake control valve 9 is provided on the intake pipe 8, a mounting bracket 10 is fixedly installed on the surface of the intake pipe 8, and the mounting bracket 10 is fixedly connected to the box body 1, a sealing groove 11 is provided at the right end of the intake pipe 8, a sealing ring 12 is inserted into the sealing groove 11, a connecting pipe 13 is fixedly installed on the right side of the sealing ring 12, the sealing ring 12 is annular in structure, and the sealing ring 12 is made of rubber material, which is convenient for connecting the connecting pipe 13 and the intake pipe 8 to have better sealing performance, a mounting ring 14 is fixedly installed on the surface of the connecting pipe 13, a limiting groove 15 is provided on the right side of the mounting ring 14, and a gas A pressure reducing valve 16 is provided, a gas tank 17 is provided at the bottom of the connecting pipe 13, a limit assembly is provided on the mounting frame 10, and the limit assembly includes a fixing frame 18 fixedly provided on the right side of the mounting frame 10, and the number of fixing frames 18 is two, and the two fixing frames 18 are symmetrically distributed. A drive motor 19 is fixedly installed on the right side of the fixing frame 18, and the output end of the drive motor 19 extends through the fixing frame 18 to the inside of the fixing frame 18 and is fixedly installed with a screw 20. The surface of the screw 20 is threadedly connected with a moving block 21, and the moving block 21 is slidably connected to the fixing frame 18. A first limit block 22 is fixedly installed on the left side of the moving block 21 for easy adjustment. The mounting ring 14 is limited, thereby facilitating the fixation of the connecting pipe 13. The sealing ring 12 on the left side of the connecting pipe 13 is inserted into the sealing groove 11 until the connecting pipe 13 fits with the intake pipe 8. The drive motor 19 is started to rotate the screw 20, so that the moving block 21 moves to the left along the fixing frame 18, so that the first limiting block 22 is inserted into the limiting groove 15, until the moving block 21 tightly squeezes the mounting ring 14, so that the installation of the connecting pipe 13 can be completed more quickly, avoiding the use of bolts for fixation, and making it easier to replace the gas tank 17 later.

[0022] Working principle: Open the box cover 4 through the handle 5, place the culture plate 3 containing cells on the base 2, close the box cover 4 through the handle 5, insert the sealing ring 12 on the left side of the connecting pipe 13 into the sealing groove 11, until the connecting pipe 13 fits with the air inlet pipe 8, start the drive motor 19, and rotate the screw 20 to make the moving block 21 move to the left along the fixed frame 18, so that the first limit block 22 is inserted into the limit groove 15 until the moving block 21 tightly squeezes the mounting ring 14. When ventilating, open the gas pressure reducing valve 16, the air inlet control valve 9 and the air outlet control valve 7, and the gas in the gas tank 17 will enter the air inlet pipe 8 from the connecting pipe 13, and then enter the box body 1, and the original gas in the box body 1 will be discharged from the air outlet pipe 6, thereby ventilating.

[0023] Example 2:

[0024] like Figure 5 As shown, a gas replacement device for cell culture, the difference between Example 2 and Example 1 lies in the difference in the limiting assembly, the limiting assembly in Example 2 is, the limiting assembly includes a support frame 23 fixedly arranged on the top of the fixed frame 18, the inner wall of the support frame 23 is fixedly installed with a fixed plate 24, the left side of the fixed plate 24 is fixedly installed with an electric push rod 25, the output end of the electric push rod 25 is fixedly installed with a movable plate 26, the left side of the movable plate 26 is fixedly installed with a second limiting block 27, the side of the movable plate 26 is fixedly installed with a slider 28, the surface of the slider 28 is slidably connected to a slide rail 29, the slider 28 is a convex structure, the slider 28 is made of metal, and the slide rail 29 is fixedly connected to the support frame 23.

[0025] The principle of this embodiment is similar to that of embodiment 1, with the main difference being that after the connecting pipe 13 is fitted with the air intake pipe 8, the electric push rod 25 is started, so that the movable plate 26 moves to the left along the slide rail 29 through the slider 28, so that the second limit block 27 moves to the left, so that the second limit block 27 is inserted into the limit groove 15 at the same time, until the movable plate 26 tightly squeezes the mounting ring 14, so that the mounting ring 14 can be fixed more stably. The principles of other parts are the same as those of embodiment 1 and will not be repeated here.

[0026] The electrical components mentioned in this article are all connected to an external main controller and 220V mains electricity, and the main controller can be a conventional known device that performs control such as a computer.

Claims

1. A gas replacement device for cell culture, characterized in that: The invention comprises a box body (1), wherein a base (2) is fixedly mounted on the inner wall of the box body (1), a culture plate (3) is overlapped on the base (2), a sealing component is arranged on the top of the box body (1), an air outlet component is arranged on the left side of the box body (1), an air inlet pipe (8) is fixedly mounted on the right side of the box body (1), an air inlet control valve (9) is arranged on the air inlet pipe (8), a mounting frame (10) is fixedly mounted on the surface of the air inlet pipe (8), and the mounting frame (10) is fixedly connected to the box body (1), and the air inlet pipe ( 8) A sealing groove (11) is provided at the right end, a sealing ring (12) is inserted into the sealing groove (11), a connecting pipe (13) is fixedly installed on the right side of the sealing ring (12), a mounting ring (14) is fixedly installed on the surface of the connecting pipe (13), a limiting groove (15) is provided on the right side of the mounting ring (14), a gas pressure reducing valve (16) is provided on the connecting pipe (13), a gas tank (17) is provided at the bottom of the connecting pipe (13), and a limiting component is provided on the mounting frame (10).

2. A gas replacement device for cell culture according to claim 1, characterized in that: The sealing assembly comprises a box cover (4) hingedly arranged on the top of the box body (1), and a handle (5) is fixedly installed on the top of the box cover (4).

3. A gas replacement device for cell culture according to claim 1, characterized in that: The air outlet assembly comprises an air outlet pipe (6) fixedly arranged on the left side of the box body (1), and an air outlet control valve (7) is arranged on the air outlet pipe (6).

4. The gas replacement device for cell culture according to claim 1, characterized in that: The limiting assembly comprises a fixing frame (18) fixedly arranged on the right side of the mounting frame (10); a driving motor (19) is fixedly installed on the right side of the fixing frame (18); an output end of the driving motor (19) passes through the fixing frame (18) and extends into the interior of the fixing frame (18) and is fixedly installed with a screw rod (20); a surface of the screw rod (20) is threadedly connected to a moving block (21); the moving block (21) is slidably connected to the fixing frame (18); and a first limiting block (22) is fixedly installed on the left side of the moving block (21).

5. A gas replacement device for cell culture according to claim 4, characterized in that: There are two fixing frames (18), and the two fixing frames (18) are symmetrically distributed.

6. The gas replacement device for cell culture according to claim 1, characterized in that: The sealing ring (12) is in a circular ring structure and is made of rubber material.

7. The gas replacement device for cell culture according to claim 1, characterized in that: The limiting assembly comprises a support frame (23) fixedly arranged on the top of the fixing frame (18), a fixing plate (24) fixedly installed on the inner wall of the support frame (23), an electric push rod (25) fixedly installed on the left side of the fixing plate (24), a movable plate (26) fixedly installed on the output end of the electric push rod (25), a second limiting block (27) fixedly installed on the left side of the movable plate (26), a slider (28) fixedly installed on the side of the movable plate (26), a slide rail (29) slidably connected to the surface of the slider (28), and the slide rail (29) is fixedly connected to the support frame (23).

8. The gas replacement device for cell culture according to claim 7, characterized in that: The slider (28) has a convex structure and is made of metal.

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