Mouse gavage device with anaerobic bacteria fermentation concentration monitoring function

Abstract

The utility model relates to the technical field of biological experiment equipment, in particular to a mouse gavage device with an anaerobic bacteria fermentation concentration monitoring function, which comprises a biological fermentation tank and further comprises a first draw-off pump, a second draw-off pump, a third draw-off pump, a fourth draw-off pump and a fourth draw-off pump, the analysis device is communicated with the first draw-off pump and is used for analyzing the concentration of the fermentation product; the second draw-off pump is communicated with the biological fermentation tank and is used for extracting a fermentation product in the biological fermentation tank for intragastric administration; one end of the stomach tube is communicated with the second draw-off pump, and a gavage needle is arranged at the other end of the stomach tube and used for injecting the fermentation product into the stomach of the mouse; the first draw-off pump, the analysis device, the second draw-off pump and the stomach tube are hermetically connected through the silica gel hose, and the gas supply device is arranged in the glass cabinet to provide controlled gas, so that the contact between anaerobic bacteria and air is reduced to the greatest extent in the extraction, analysis and gavage processes, and the activity and inhibition effect of the anaerobic bacteria are effectively maintained.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experimental equipment, in particular to a mouse gavage device capable of monitoring the concentration of anaerobic bacteria fermentation. Background Art

[0002] Mouse gavage testing is a commonly used experimental method in biological and medical research. It is primarily used to orally introduce drugs, nutrients, or other test substances into mice to observe their physiological, pathological, or metabolic effects. In some studies, anaerobic bacteria (which are sensitive to oxygen and die upon exposure) are administered via gavage to artificially establish bacterial colonization or infection models in target mice, or to evaluate the effects of a substance on mice.

[0003] In the prior art, these anaerobic bacteria usually need to be cultured in a sealed fermentation tube or bio-fermentation tank by adding a suitable liquid culture medium. After the culture is completed, it needs to be extracted and then injected into the stomach of the mouse through a gastric tube. However, in the process of removing the anaerobic bacteria from the sealed container and transferring them to the gastric tube, they are often inevitably exposed to the air. Since anaerobic bacteria are highly sensitive to oxygen, this exposure will cause a large number of bacteria to die or their activity will be significantly reduced, which will seriously affect the subsequent establishment of bacterial colonization in the mouse body or the effect of exerting the expected effect, thereby affecting the accuracy and reliability of the experimental results.

[0004] In view of the above problems, the existing technology is in urgent need of improvement. Utility Model Content

[0005] The purpose of the utility model is to solve the shortcomings of the prior art and to propose a mouse gavage device with the function of monitoring anaerobic bacteria fermentation concentration.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is: a mouse gavage device with anaerobic fermentation concentration monitoring function, comprising a biological fermentation tank for anaerobic fermentation, and further comprising:

[0007] a first extraction pump, connected to the bio-fermentation tank, for extracting the fermentation product in the bio-fermentation tank for analysis;

[0008] an analysis device, connected to the first extraction pump, for analyzing the concentration of the fermentation product;

[0009] a second extraction pump, connected to the bio-fermentation tank, for extracting the fermentation product in the bio-fermentation tank for gavage;

[0010] a gastric tube, one end of which is connected to the second extraction pump and the other end of which is provided with a gavage needle for injecting the fermentation product into the stomach of the mouse;

[0011] Two silicone hoses, respectively connecting the first extraction pump and the analysis device and the second extraction pump and the gastric tube, to form sealed connection channels;

[0012] A glass cabinet with a one-way valve is used to accommodate the biological fermentation tank and the analysis device, and the silicone hose connected to the gastric tube is sealed and fixed to the glass cabinet;

[0013] The gas supply device is communicated with the glass cabinet and is used for supplying mixed gas into the glass cabinet.

[0014] Furthermore, the first extraction pump and the second extraction pump are both peristaltic pumps.

[0015] More specifically, the silicone hose is connected to the first extraction pump, the analysis device, the second extraction pump and the gastric tube through a sterile quick connector.

[0016] Preferably, a flow regulating valve is installed on the gastric tube.

[0017] Furthermore, a cabinet door is slidably installed on the top of the glass cabinet through a first sliding groove and a second sliding groove respectively.

[0018] The two cabinet doors are slidably arranged in the corresponding first sliding groove and the second sliding groove through a sliding block.

[0019] The two cabinet doors are arranged in parallel and staggered.

[0020] A sealing gasket is provided between the two cabinet doors.

[0021] Furthermore, the first extraction pump and the second extraction pump inject gas into the biological fermentation tank when extracting the fermentation product.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The first extraction pump, analysis device, second extraction pump and gastric tube are sealed and connected by a silicone hose, and a gas supply device is set in the glass cabinet to provide controlled gas, thereby minimizing the contact between anaerobic bacteria and air during the extraction, analysis and gavage processes, effectively maintaining the activity and inhibitory effect of anaerobic bacteria, and solving the technical problem in the prior art that the activity of anaerobic bacteria is reduced due to exposure to air during gavage. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the overall structure of a mouse gavage device that can also monitor anaerobic fermentation concentration.

[0025] Figure 2 This is a schematic diagram of the structure of a biological fermentation tank in a mouse gavage device that also monitors anaerobic fermentation concentration.

[0026] Figure 3This is a schematic diagram of the structure of the first chute and the second chute in a mouse gavage device that also monitors anaerobic fermentation concentration.

[0027] Figure 4 This is a schematic diagram of the gastric tube structure in a mouse gavage device that also monitors anaerobic fermentation concentration.

[0028] Figure 5 This is a schematic diagram of the cabinet door structure of a mouse gavage device that also monitors anaerobic fermentation concentration.

[0029] In the figure: 1. Biological fermentation tank; 2. First extraction pump; 3. Analysis device; 4. Second extraction pump; 5. Gastric tube; 6. Gavage needle; 7. Silicone hose; 8. Flow regulating valve; 9. Glass cabinet; 10. Gas supply device; 11. First slide; 12. Second slide; 13. Slider; 14. Cabinet door; 15. Sealing gasket. DETAILED DESCRIPTION

[0030] The following description is intended to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments described below are merely examples, and those skilled in the art may conceive of other obvious variations.

[0031] In the field of biological and medical research, mouse gavage experiments are an important research method, especially widely used in the study of intestinal flora, drug metabolism, etc. For some anaerobic bacteria that are sensitive to oxygen, they need to be cultured and operated in a strict anaerobic environment. However, in the process of transferring anaerobic bacteria from the culture container to the mouse stomach, the existing gavage device often cannot avoid the anaerobic bacteria from being exposed to the air, resulting in reduced activity or even death, which seriously affects the accuracy of the experimental results. In order to solve this technical problem, the utility model discloses a mouse gavage device that can monitor the fermentation concentration of anaerobic bacteria. The device minimizes the contact between anaerobic bacteria and air by constructing a sealed transmission and operation environment, thereby maintaining their activity and improving the success rate and reliability of the experiment.

[0032] like Figures 1 to 5 The mouse gavage device shown here, which is also capable of monitoring the concentration of anaerobic fermentation, comprises a biological fermentation tank 1 for performing anaerobic fermentation, and further comprises:

[0033] a first extraction pump 2, connected to the bio-fermentation tank 1, for extracting the fermentation product in the bio-fermentation tank 1 for analysis;

[0034] an analysis device 3, connected to the first extraction pump 2, for analyzing the concentration of the fermentation product;

[0035] The second extraction pump 4 is connected to the biological fermentation tank 1 and is used to extract the fermentation product in the biological fermentation tank 1 for gavage;

[0036] A stomach tube 5, one end of which is connected to the second extraction pump 4, and the other end of which is provided with a gavage needle 6 for injecting the fermentation product into the stomach of the mouse;

[0037] Two silicone hoses 7 are respectively connected to the first extraction pump 2 and the analysis device 3 and to the second extraction pump 4 and the gastric tube 5 to form sealed connection channels;

[0038] A glass cabinet 9 with a one-way valve is used to accommodate the biological fermentation tank 1 and the analysis device 3. The silicone hose 7 connected to the gastric tube 5 is sealed and fixed to the glass cabinet 9;

[0039] The gas supply device 10 is in communication with the glass cabinet 9 and is used to supply mixed gas into the glass cabinet 9 .

[0040] Compared with the prior art, the key innovation of the present invention is to construct a sealed transmission system for the entire process from fermentation, monitoring to gavage, and to use a glass cabinet with a controlled atmosphere. In the prior art, when taking out anaerobic bacteria from a fermentation tank for analysis or gavage, it is often necessary to open the container or connecting pipe, which causes the anaerobic bacteria to be exposed to the air and the activity to drop rapidly. However, the present invention uses a silicone hose 7 to seal and connect each key component, and provides an anaerobic or hypoxic mixed gas environment in the glass cabinet 9, so that the fermentation product is always isolated from the outside air during the process of extraction, analysis and delivery to the gastric tube 5. In particular, the sealing and fixation between the silicone hose 7 connecting the gastric tube 5 and the glass cabinet 9 ensures that when the gastric tube 5 is extended out of the cabinet for gavage, the controlled atmosphere inside the glass cabinet 9 will not leak in large quantities and outside air will not enter. This design greatly reduces the contact between anaerobic bacteria and oxygen, effectively maintains the activity of anaerobic bacteria, and thus improves the success rate of mouse gavage experiments and the reliability of research results.

[0041] When using the device of the present invention to conduct a mouse gavage experiment, first, liquid culture medium and anaerobic bacteria are added to the biological fermentation tank 1 for anaerobic fermentation. During the fermentation process, if it is necessary to monitor the concentration of the fermentation product, a small amount of fermentation product in the biological fermentation tank 1 can be extracted by the first extraction pump 2. The product is transported to the analysis device 3 through the silicone hose 7 (by injecting the fermentation product into a test tube, centrifuging it through a centrifuge, filtering out residual bacteria and spores through a filter membrane, and then detecting it through a gas chromatography-mass spectrometry instrument) for analysis. During the analysis, the fermentation product is placed in the glass cabinet 9. A suitable mixed gas is introduced through a gas supply device 10, facilitating the expulsion of air from the glass cabinet 9 through its one-way valve, maintaining an anaerobic environment during the testing process. (The mixed gas can be 85% N2 + 10% H2 + 5% CO2. This 85% N2 + 10% H2 + 5% CO2 mixture is a commonly used anaerobic or microaerobic culture gas, particularly suitable for cultivating oxygen-sensitive anaerobic bacteria.) The entire extraction and delivery process takes place within a sealed conduit, with the bio-fermentation tank 1 and analysis device 3 located in a controlled atmosphere within the glass cabinet 9, preventing air contact. When the fermentation product concentration reaches the required level, the fermentation product within the bio-fermentation tank 1 can be extracted using a second extraction pump 4. This fermentation product is then delivered to a gastric tube 5 via a silicone hose 7 and injected into the mouse's stomach via a gavage needle 6 at the end of the tube 5.

[0042] The passage connecting the second extraction pump 4, the silicone hose 7 and the gastric tube 5 is sealed, and the silicone hose 7 extends to the outside through the sealed fixing point of the glass cabinet 9, ensuring that the entire transmission process from the biological fermentation tank 1 to the mouse stomach is carried out under the condition of maximum air isolation. The biological fermentation tank 1 is used to provide a culture environment for anaerobic bacteria. The first extraction pump 2 and the analysis device 3 work together to monitor the fermentation process without destroying the anaerobic environment. The second extraction pump 4 and the gastric tube 5 constitute the path for gavage to the mouse. The silicone hose 7 is the key to achieving a sealed connection of the entire system. The glass cabinet 9 and the gas supply device 10 jointly provide and maintain the necessary controlled gas environment for the biological fermentation tank 1 and the analysis device 3. Through the synergistic effect of these components, the utility model effectively solves the technical problem of reduced activity of anaerobic bacteria during gavage, and provides a powerful tool for related research.

[0043] As an embodiment of the present invention, the first extraction pump 2 and the second extraction pump 4 are both peristaltic pumps.

[0044] As an embodiment of the present invention, the silicone hose 7 is connected to the first extraction pump 2, the analysis device 3, the second extraction pump and the gastric tube 5 through a sterile quick connector.

[0045] During implementation, the present invention further enhances the sealing and sterility of the entire fluid transfer system by using sterile quick connectors to connect the silicone hoses. This is crucial for ensuring that the entire process of extraction from the bio-fermentation tank 1, analysis, and gavage is carried out under a strictly controlled environment. This effectively prevents anaerobic bacteria from being contaminated or exposed to oxygen during operation, thereby better maintaining their activity and function, and improving the reliability and success rate of the experiment.

[0046] As an embodiment of the present invention, a flow regulating valve 8 is installed on the gastric tube 5; installing the flow regulating valve 8 on the gastric tube 5 significantly improves the accuracy and safety of the gastric gavage operation.

[0047] In one embodiment of the present invention, cabinet doors 14 are slidably mounted above the glass cabinet 9 via first and second slide grooves 11, 12, respectively. The two cabinet doors 14 are slidably mounted within their respective first and second slide grooves 11, 12 via sliders 13. The two cabinet doors 14 are arranged in a parallel, staggered arrangement. A sealing gasket 15 is disposed between the two cabinet doors 14.

[0048] During implementation, the sliding installation of the cabinet door 14 is achieved through the first and second sliding grooves 11, 12, allowing operators to easily slide the cabinet door 14 to enter the glass cabinet 9 to perform operations, such as placing or removing the biological fermentation tank 1 and the analysis device 3, or performing maintenance work such as connecting pipes. At the same time, when the sliding installation structure is properly designed, when the cabinet door 14 is closed, it can be tightly attached to the cabinet edge of the glass cabinet 9, helping to maintain the sealing of the internal environment of the glass cabinet 9 and reducing the leakage of the internal mixed gas and the ingress of external air.

[0049] A sealing gasket 15 is provided between the contacting or overlapping areas of the two cabinet doors 14 and is arranged along the edges or overlapping portions of the cabinet doors 14. This further enhances the sealing performance of the glass cabinet 9, ensuring that the controlled atmosphere inside the glass cabinet 9 is stably maintained, minimizing the risk of anaerobic bacteria being exposed to oxygen during operation within the glass cabinet 9. This contributes significantly to maintaining the activity of anaerobic bacteria and improving the reliability of experimental results, representing an effective improvement to the glass cabinet sealing structure.

[0050] As an embodiment of the present invention, the first extraction pump 2 and the second extraction pump 4 inject gas into the biological fermentation tank 1 to maintain air pressure balance when extracting fermentation products.

[0051] In practice, gas injection can be achieved in a variety of ways. For example, a gas port can be provided near the liquid inlet of the first extraction pump 2 and the second extraction pump 4. Gas can be introduced through this port and delivered to the bio-fermentation tank 1 when the pumps are operating. Alternatively, an independent gas inlet can be provided on the bio-fermentation tank 1, and a control system can be used to replenish gas into the tank through this gas inlet when the pumps are extracting liquid. The injected gas can be other inert gases.

[0052] Thus, by injecting gas into the bio-fermentation tank 1 when extracting the fermentation product, the utility model effectively solves the negative pressure problem that may be caused by extracting liquid in a closed container. When the liquid is extracted, if the gas is not replenished, the air pressure inside the bio-fermentation tank 1 will drop, which may make extraction difficult, affect the working efficiency of the pump, and even have an adverse effect on the growth environment of anaerobic bacteria. By synchronously injecting gas, the air pressure balance inside the bio-fermentation tank 1 can be maintained, ensuring the smoothness and stability of the liquid extraction process, preventing interference with the fermentation process or subsequent operations due to pressure changes, and improving the stability and reliability of the entire system.

[0053] Working principle of this utility model:

[0054] By adding liquid culture medium and Akkermansia into a bio-fermentation tank 1 for fermentation, an AB colonization or infection model can be artificially established in target mice during fermentation (especially by oral gavage of AB bacterial liquid). After a certain fermentation period, a certain amount of product in the bio-fermentation tank 1 can be extracted by a first extraction pump 2 and tested by an analysis device 3 (by injecting the fermentation product into a test tube, centrifuging it, filtering out residual bacteria and spores through a filter membrane, and then detecting it through a gas chromatography-mass spectrometry) to detect the concentration of short-chain fatty acids (SCFAS). When the concentration is sufficient, the fermented product in the bio-fermentation tank 1 can be extracted by a second extraction pump 4, injected into a gastric tube 5, and then injected into the stomach of the mouse through a gavage needle 6 installed on one side of the gastric tube 5. 48 hours after gavage, the mouse feces / colon contents are collected for quantitative culture and detection of AB-specific genes to detect whether Akkermansia can effectively inhibit AB.

[0055] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions only describe the principles of the present invention. Various changes and improvements are possible without departing from the spirit and scope of the present invention. Such changes and improvements fall within the scope of the present invention as claimed, and the scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A mouse gavage device with the function of monitoring anaerobic fermentation concentration, comprising a biological fermentation tank (1) for performing anaerobic fermentation, characterized in that: Also includes: a first extraction pump (2), connected to the biological fermentation tank (1), for extracting the fermentation product in the biological fermentation tank (1) for analysis; an analysis device (3), connected to the first extraction pump (2), for analyzing the concentration of the fermentation product; a second extraction pump (4), connected to the biological fermentation tank (1), for extracting the fermentation product in the biological fermentation tank (1) for gavage; A gastric tube (5), one end of which is connected to the second extraction pump (4), and the other end of which is provided with a gavage needle (6) for injecting the fermentation product into the stomach of the mouse; Two silicone hoses (7) are respectively connected to the first extraction pump (2) and the analysis device (3) and the second extraction pump (4) and the gastric tube (5), forming a sealed connection channel; A glass cabinet (9) with a one-way valve is used to accommodate the biological fermentation tank (1) and the analysis device (3), and a silicone hose (7) connected to the gastric tube (5) is sealed and fixed to the glass cabinet (9); A gas supply device (10) is in communication with the glass cabinet (9) and is used to supply mixed gas into the glass cabinet (9).

2. The mouse gavage device for monitoring anaerobic fermentation concentration according to claim 1, characterized in that: The first extraction pump (2) and the second extraction pump (4) are both peristaltic pumps.

3. The mouse gavage device with anaerobic fermentation concentration monitoring function according to claim 1, characterized in that: The silicone hose (7) is connected to the first extraction pump (2), the analysis device (3), the second extraction pump, and the gastric tube (5) via a sterile quick connector.

4. The mouse gavage device for monitoring anaerobic fermentation concentration according to claim 1, characterized in that: A flow regulating valve (8) is installed on the gastric tube (5).

5. The mouse gavage device with anaerobic bacteria fermentation concentration monitoring function according to claim 1, characterized in that: A cabinet door (14) is slidably mounted above the glass cabinet (9) via a first sliding groove (11) and a second sliding groove (12).

6. The mouse gavage device for monitoring anaerobic fermentation concentration according to claim 5, characterized in that: The two cabinet doors (14) are slidably arranged in the corresponding first sliding groove (11) and the second sliding groove (12) via a slider (13).

7. The mouse gavage device for monitoring anaerobic fermentation concentration according to claim 6, characterized in that: The two cabinet doors (14) are arranged in parallel and staggered.

8. The mouse gavage device for monitoring anaerobic fermentation concentration according to claim 7, characterized in that: A sealing gasket (15) is provided between the two cabinet doors (14).

9. The mouse gavage device with anaerobic bacteria fermentation concentration monitoring function according to claim 1, characterized in that: The first extraction pump (2) and the second extraction pump (4) inject gas into the biological fermentation tank (1) when extracting fermentation products.

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