Automatic filling device and biological tissue transferring and filling device

Abstract

The utility model provides an automatic filling device and a biological tissue transferring and filling device, and the automatic filling device comprises one or more negative pressure ports which are arranged on one wall of the automatic filling device, the one or more negative pressure ports are connected with liquid or air to be sucked through one or more sleeves; and the negative pressure device is used for filling liquid or air into the one or more sleeves through the one or more negative pressure ports. According to the casing pipe filling device, the casing pipe filling efficiency is effectively improved, and the casing pipe filling complexity is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of bioengineering, in particular to an automatic filling device and a biological tissue transfer and filling device. Background Art

[0002] Despite the remarkable success of cryopreservation technology, particularly vitrification, in human medicine and its widespread application in reproductive medicine, its application in the livestock industry has been significantly limited. This limitation is primarily due to factors such as cost, expertise, and farmland environment. Vitrification requires expensive equipment and expertise, which can be impractical for the livestock industry. Specifically, embryologists need to manually fill straws with one or more sections of culture medium, a process that requires meticulous training and a specialized laboratory environment. In farm settings, personnel with these specialized skills are often lacking, making the application of this technology difficult.

[0003] Prior art requires skilled embryologists to transfer embryos. After thawing, embryos need to be transferred to recipient cows as quickly as possible. However, due to poor pasture conditions, manually filling straws with liquid is not only slow, but also difficult to achieve uniform filling volume and location. It also fails to ensure sterility, resulting in a low success rate. Furthermore, manual embryo transfer and straw filling are cumbersome, inefficient, and prone to errors. Utility Model Content

[0004] The main purpose of the utility model is to provide an automated filling device and a biological tissue transfer and filling device to solve the problems of the prior art in that manual filling of liquid into straws is complicated and inefficient.

[0005] According to an embodiment of the present invention, an automated filling device is proposed, which includes: one or more negative pressure ports, which are arranged on a wall of the automated filling device, and the one or more negative pressure ports are connected to the liquid or air to be sucked through one or more sleeves; a negative pressure device, which fills the liquid or air into the one or more sleeves through the one or more negative pressure ports.

[0006] Each sleeve includes multiple sections of liquid, and air is spaced between the multiple sections of liquid.

[0007] Wherein, at least one section of the multiple sections of liquid contains biological tissue.

[0008] Wherein, the biological tissue includes an embryo or an egg.

[0009] Each sleeve includes multiple sections of liquid, and the number of the multiple sections of liquid in each sleeve is equal.

[0010] Wherein, the number of the one or more negative pressure ports is 1-3.

[0011] Wherein, the negative pressure device is a syringe pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, an impeller pump or a piston pump.

[0012] Wherein, the liquid is culture fluid, transplantation fluid or operating fluid.

[0013] The automatic filling device further comprises: a heating platform, which is arranged on the upper surface of the automatic filling device, and comprises a surface for placing a dish container and a container for holding liquid.

[0014] Wherein, the automatic filling device further includes: a stirring device, which is arranged below the container containing the liquid and is a magnetic stirrer.

[0015] According to an embodiment of the present invention, a biological tissue transfer and loading device is also proposed, which includes: an automated loading device, the automated loading device including one or more negative pressure ports and a negative pressure device, the one or more negative pressure ports being connected to one or more cannulas; a clamp, the clamp clamping one or more carrier rods carrying biological tissue; wherein the automated loading device, the clamp and the carrier rod are used in combination to complete the transfer and loading of biological tissue.

[0016] In which, the biological tissue transfer and loading device includes a loading state and a transfer state. When the biological tissue transfer device is in the loading state, the one or more negative pressure ports are connected to the liquid or air to be absorbed through one or more cannulas, and the negative pressure device fills the liquid or air into the one or more cannulas through the one or more negative pressure ports; when the biological tissue transfer and loading device is in the transfer state, the ends of the one or more carrier rods are opposite to the ends of the one or more cannulas, and the clamp drives the one or more carrier rods to move axially, so that the biological tissue contained in the one or more carrier rods is transferred to the corresponding one or more cannulas.

[0017] Wherein, the biological tissue includes an embryo or an egg.

[0018] According to the technical solution of the present invention, by providing one or more negative pressure ports, the liquid or air to be drawn is connected to the negative pressure ports through multiple cannulas, and the negative pressure device automatically fills the liquid or air to be drawn into the cannula through the negative pressure ports, without the need for complex professional knowledge and delicate operation of the embryologist, thereby effectively improving the filling efficiency of the cannula and reducing the complexity of filling the cannula. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0020] Figure 1 is a schematic diagram of an automated filling device according to an embodiment of the present invention;

[0021] Figure 2 is a schematic diagram of the internal structure of an automated filling device according to an embodiment of the present utility model;

[0022] Figure 3A is a schematic diagram of a biological tissue transfer device according to an embodiment of the present invention;

[0023] Figure 3B yes Figure 3A A magnified view of a part in the middle. DETAILED DESCRIPTION

[0024] To make the purpose, technical solutions, and advantages of the present invention more clear, the following will provide a clear and complete description of the technical solutions of the present invention in conjunction with specific embodiments of the present invention and the corresponding drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are also within the scope of protection of the present invention.

[0025] The technical solutions provided by various embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0026] According to an embodiment of the present invention, an automatic filling device is provided, which has a closed shell. Figure 1 and Figure 2 The automated filling device 10 includes: one or more negative pressure ports 11 provided on a wall of the housing, a negative pressure device 12 provided within the housing, and one or more cannulas (not shown). The one or more negative pressure ports 11 are provided on any wall of the housing, and the number of negative pressure ports 11 may be 1-3. One end of the one or more cannulas is connected to the negative pressure port 11, and the other end of the one or more cannulas is connected to an external test tube or air, wherein the external test tube contains the liquid to be drawn. The negative pressure device 12 is connected to the negative pressure port 11, and the negative pressure device 12 draws the liquid or air from the external test tube through negative pressure and fills the liquid or air into the cannula to form one or more sections of liquid in the cannula. Specifically, the negative pressure device 12 may include at least one pump structure, which may be one or more of a syringe pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, an impeller pump, or a piston pump.

[0027] In some embodiments of the present application, the number of negative pressure ports 11 is 3, so the negative pressure device 12 may include 1 or 3 pump structures. When the negative pressure device 12 includes 1 pump structure, the pump structure can be connected to the 3 negative pressure ports 11 through a multi-way connector; when the negative pressure device 12 includes 3 pump structures, the 3 pump structures are respectively connected to the 3 negative pressure ports 11. A support frame for supporting the multi-way connector can also be provided below the multi-way connector. In other embodiments of the present application, the number of negative pressure ports 11 can also be 1. In this case, the negative pressure device 12 includes 1 pump structure, and the negative pressure port 11 can be connected to multiple sleeves through a multi-way connector.

[0028] refer to Figure 1 The automated filling device 10 also includes a heating platform 13, which is located on the upper surface of the housing of the automated filling device 10 and includes a horizontal surface 131 and a container 132 for holding liquid (e.g., a beaker). A heating device or heating plate (not shown) is located below the heating platform 13. The heating device can heat liquid materials such as the solution and / or thawing oil required for embryo transfer. These liquid materials can be placed on the surface 131 and / or in the container 132 via a dish container. A temperature sensor (not shown) can also be located on the heating platform to maintain a constant temperature over a long period of time. A stirring device 14 can also be located below the container 132. The stirring device 14 can be a magnetic stirrer that stirs the liquid material in the container while the heating device heats it, thereby preventing uneven heating of the liquid material.

[0029] Furthermore, the automated filling device 10 may be equipped with an electronic control device, which may be a circuit board, to adjust the aspiration speed and volume of each liquid segment, the heating temperature of the heating table, and the speed of the stirring device according to actual needs. A display screen for displaying these parameters and control buttons for adjusting these parameters may also be provided on the top surface of the automated filling device 10.

[0030] In actual use, the automated filling device 10 is first connected to an external test tube via a cannula. The automated filling device 10 draws liquid from the external test tube into the cannula, thereby forming a liquid segment in each cannula. The automated filling device 10 is then connected to a gas via the cannula. The automated filling device 10 draws gas and fills it into the cannula, thereby forming a gas segment in each cannula. This process is repeated to fill the cannula with multiple liquid segments, each separated by a gas, which can be air. Furthermore, the number of liquid segments in each cannula is equal, specifically three or five, and at least one liquid segment in each cannula contains biological tissue.

[0031] According to the embodiment of the present invention, a biological tissue transfer and filling device is also provided, which can automatically realize the filling of the cannula and the transfer of biological tissue. Figure 3A and Figure 3B The biological tissue transfer and loading device includes: an automated loading device 10 and a clamp 20. Since the structure of the automated loading device 10 has been described in detail above, it will not be repeated here. The clamp 20 includes: a first clamping part 21 and a second clamping part 22. The first clamping part 21 can move axially relative to the second clamping part 22. One or more carrier rods 30 are clamped in the first clamping part 21, and one or more sleeves 40 are clamped in the second clamping part 22. The front end of the carrier rod 30 carries the biological tissue to be transferred, which can be an embryo or an egg. The negative pressure port 11 of the automated loading device 10 is connected to one end of the sleeve 40 through a connecting tube, and the automated loading device 10 is also electrically connected to the clamp 20.

[0032] The biological tissue transfer device includes a loading state and a transfer state. When the biological tissue transfer device is in the loading state, the other end of the sleeve 40 is connected to an external test tube or air, and the negative pressure device 12 fills liquid or air into the sleeve 40 through the negative pressure port 11; when the biological tissue transfer device is in the transfer state, the other end of the sleeve 40 is arranged opposite to the front end of the carrier rod 30, and the automated loading device 10 controls the axial movement of the first clamping part 21, and the first clamping part 21 drives the carrier rod 30 to move axially, so that the front end of the carrier rod 30 docks or contacts with the sleeve 40, and the biological tissue in the carrier rod 30 falls off and is transferred to a section of liquid in the sleeve 40.

[0033] In actual use, three sections of liquid are first filled into the cannula through a negative pressure device, and then the biological tissue is transferred from the front end of the carrier rod to the appropriate position of the cannula. Specifically, the biological tissue can be located in any section of liquid, so that at least one section of liquid in the cannula contains biological tissue. Finally, two sections of liquid are filled into the cannula through the negative pressure device, thereby forming five sections of liquid in the cannula, so as to facilitate the subsequent transfer of the biological tissue into the target animal.

[0034] According to an embodiment of the present application, a negative pressure device and a negative pressure port connected to the negative pressure device are provided. The negative pressure port is connected to the liquid or air to be drawn through a cannula. The negative pressure device automatically fills or loads the liquid to be drawn into the cannula, thereby forming at least one section of liquid in the cannula. Using this device does not require complex professional knowledge and delicate operation, effectively improving the efficiency of cannula filling and reducing the complexity of the filling operation. Furthermore, by providing a heating device and a stirring device in the automated filling device, there is no need to prepare other operating devices required for biological tissue transfer, further improving work efficiency.

[0035] Although the present disclosure has been described in detail with reference to specific embodiments thereof, those skilled in the art will appreciate that various changes and modifications may be made therein without departing from the spirit and scope of the embodiments. Therefore, the present disclosure is intended to cover modifications and variations of the present disclosure, and any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present disclosure shall be included within the scope of the claims of the present disclosure and their equivalents.

[0036] In addition, the features disclosed in the above description or claims or drawings, in their specific forms or according to the manner for performing the disclosed functions or the method or process for obtaining the disclosed results, can be used alone or in any combination of these features to implement the present invention in their different forms. Specifically, one or more features of any embodiment described herein can be combined with one or more features of any other embodiment described herein.

[0037] Protection may also be sought for any features disclosed in any one or more of the publications cited in conjunction with the present disclosure and / or incorporated by reference.

Claims

1. An automatic filling device, characterized in that: include: One or more negative pressure ports, the one or more negative pressure ports being arranged on a wall of the automated filling device, the one or more negative pressure ports being connected to the liquid or air to be sucked through one or more cannulas; A negative pressure device is provided for filling liquid or air into the one or more sleeves through the one or more negative pressure ports.

2. The automatic filling device according to claim 1, characterized in that: Each casing includes multiple sections of liquid, and air is spaced between the multiple sections of liquid.

3. The automatic filling device according to claim 2, characterized in that: At least one section of the multiple sections of liquid contains biological tissue.

4. The automatic filling device according to claim 3, characterized in that: The biological tissue includes an embryo or an egg.

5. The automatic filling device according to claim 1, characterized in that: Each casing includes multiple sections of liquid, and the number of the multiple sections of liquid in each casing is equal.

6. The automatic filling device according to claim 1, characterized in that: The number of the one or more negative pressure ports is 1-3.

7. The automatic filling device according to claim 1, characterized in that: The negative pressure device is a syringe pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, an impeller pump or a piston pump.

8. The automatic filling device according to claim 1, characterized in that: The liquid is a culture fluid, a transplantation fluid or an operating fluid.

9. The automatic filling device according to claim 1, characterized in that: Also includes: A heating platform is provided on the upper surface of the automatic filling device, and the heating platform includes a surface for placing a dish container and a container for placing liquid.

10. The automatic filling device according to claim 9, characterized in that: Also includes: The stirring device is arranged below the container for holding the liquid, and the stirring device is a magnetic stirrer.

11. A biological tissue transfer and loading device, characterized in that: include: An automated filling device, the automated filling device comprising: one or more negative pressure ports and a negative pressure device, the one or more negative pressure ports being connected to one or more cannulas; A clamp, which clamps one or more carrier rods carrying biological tissue; The automated loading device, the clamp and the carrier rod are used in combination to complete the transfer and loading of biological tissues.

12. The biological tissue transfer and loading device according to claim 11, characterized in that: The biological tissue transfer and loading device includes a loading state and a transfer state. When the biological tissue transfer and loading device is in the loading state, the one or more negative pressure ports are connected to the liquid or air to be absorbed through one or more cannulas, and the negative pressure device fills the liquid or air into the one or more cannulas through the one or more negative pressure ports; when the biological tissue transfer and loading device is in the transfer state, the ends of the one or more carrier rods are opposite to the ends of the one or more cannulas, and the clamp drives the one or more carrier rods to move axially, so that the biological tissue contained in the one or more carrier rods is transferred to the corresponding one or more cannulas.

13. The biological tissue transfer and loading device according to claim 11, characterized in that: The biological tissue includes an embryo or an egg.

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