Glass slide for detection, glass slide assembly and microscope

Abstract

The utility model relates to a glass slide for detection, a glass slide assembly and a microscope, and the glass slide comprises a bottom plate which is provided with a groove; the bearing plate is located in the groove, a gap exists between the edge of the bearing plate and the side wall of the groove, a plurality of bearing holes are formed in the bearing plate, and the bearing holes are used for containing objects to be detected. According to the glass slide for detection, the glass slide assembly and the microscope provided by the utility model, the volatilization speed of moisture on the glass slide can be slowed down.

Description

Technical Field

[0001] The utility model relates to the technical field of glass slide structures, in particular to a glass slide for detection, a glass slide assembly and a microscope. Background Art

[0002] Microscopes are commonly used biomedical instruments that magnify tiny objects visible to the naked eye. Currently, microscopes typically use a glass slide assembly consisting of a flat glass slide and a cover glass. When the temperature of the glass slide assembly is high, moisture between the slide and cover glass evaporates rapidly, affecting the observation of biological samples and making it difficult to perform long-term, high-throughput imaging observations of sample matrices. Utility Model Content

[0003] Therefore, the technical problem to be solved by the present invention is to provide a glass slide, a glass slide assembly and a microscope for detection, which can slow down the volatilization rate of water on the glass slide.

[0004] In order to solve the above technical problems, the utility model provides a glass slide for detection, including: a base plate, which is provided with a groove; a supporting plate, which is located in the groove, and there is a gap between the edge of the supporting plate and the side wall of the groove, and the supporting plate is provided with multiple supporting holes, which are used to accommodate the object to be detected.

[0005] In one embodiment of the present invention, the center line of the bearing hole is perpendicular to the plane where the bottom plate is located.

[0006] In one embodiment of the present invention, a hydrophobic coating is provided in the gap between the carrying plate and the side wall of the groove.

[0007] In one embodiment of the present invention, the plurality of bearing holes are arranged in an equidistant array, the center distance between adjacent bearing holes is 1.5-2.5 mm, the diameter of the bearing hole is 1-1.5 mm, and the depth of the bearing hole is 0.09-0.11 mm.

[0008] In one embodiment of the present invention, a plurality of markings are provided on the bottom plate along the edge of the carrying plate, wherein the plurality of markings facing the first direction are digital markings, and the plurality of markings facing the second direction are letter markings.

[0009] In one embodiment of the present invention, the thickness of the carrying plate is smaller than the depth of the groove.

[0010] In one embodiment of the present invention, the plurality of bearing holes are arranged along at least one direction, and the diameters of the plurality of bearing holes gradually increase.

[0011] The present invention also provides a glass slide assembly, comprising the above-mentioned detection slide and a cover glass.

[0012] In one embodiment of the present invention, the cover glass is located on the carrying plate.

[0013] The present invention also provides a microscope, comprising the above-mentioned slide assembly.

[0014] The above technical solution of the utility model has the following advantages compared with the prior art:

[0015] The utility model describes a glass slide, a glass slide assembly and a microscope for detection, in which the cover glass fits into the liquid in the gap, so that the liquid can seal the gap between the cover glass and the carrier plate, thereby causing the moisture in the carrier hole to evaporate more slowly through the gap between the cover glass and the carrier plate. At the same time, through the setting of the carrier hole, more detection liquid can be injected into a single detection position, the object to be detected in the detection hole can also be kept moist for a long time, and the volatilization rate of the moisture in the carrier hole can be slowed down. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to make the content of the utility model easier to understand, the utility model is further described in detail below based on the specific embodiments of the utility model and in conjunction with the accompanying drawings, wherein

[0017] Figure 1 This is a structural schematic diagram of a glass slide for detection in the utility model;

[0018] Figure 2 yes Figure 1 Schematic diagram of the structure after adding marks;

[0019] Figure 3 It is a structural schematic diagram of the cover glass in Example 2.

[0020] Explanation of the reference numerals in the specification: 1. Base plate; 2. Cover glass; 11. Carrying plate; 12. Groove; 13. Carrying hole; 14. Number label; 15. Letter label. DETAILED DESCRIPTION

[0021] The present invention will be further described below with reference to the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and implement it. However, the embodiments are not intended to limit the present invention.

[0022] Example 1

[0023] Reference Figure 1As shown, the utility model is a glass slide for detection, comprising: a base plate 1, the base plate 1 is provided with a groove 12; a carrying plate 11, the carrying plate 11 is located in the groove 12, there is a gap between the edge of the carrying plate 11 and the side wall of the groove 12, and the carrying plate 11 is provided with a plurality of carrying holes 13, the carrying holes 13 are used to accommodate the object to be detected.

[0024] In this embodiment of a testing glass slide, objects to be tested are placed in respective bearing holes 13, and testing liquid is injected into the bearing holes 13. Simultaneously, liquid is injected into the gap between the carrier plate 11 and the sidewall of the groove 12. Finally, a cover glass 2 is placed on the carrier plate 11. The cover glass 2 conforms to the liquid in the gap, allowing the liquid to block the gap between the cover glass 2 and the carrier plate 11. This slows the rate at which moisture in the bearing holes 13 evaporates through the gap between the cover glass 2 and the carrier plate 11. Furthermore, the provision of the bearing holes 13 allows more testing liquid to be injected into a single testing position, allowing the objects to be tested in the testing holes to remain moist for a longer period of time, and also slows the rate at which moisture in the bearing holes 13 evaporates.

[0025] The base plate 1 is generally flat. Specifically, the thickness of the base plate 1 is 0.15-0.2 mm and the base plate 1 is composed of transparent borosilicate glass. When the detection slide of this embodiment is used in a laser scanning microscope, the borosilicate glass material facilitates the passage of optical signals without affecting the biological sample being detected or interfering with the detection, thereby improving detection accuracy and data processing standards. The base plate 1 is rectangular, with a rectangular groove 12 defined in the center.

[0026] The carrier plate 11 is rectangular, and the carrier plate 11 is located in the groove 12, and the carrier plate 11 is located in the middle of the groove 12. There is a gap between the edge of the carrier plate 11 and the side wall of the groove 12, and the width of the gap is equal at all positions. Liquid can be accommodated in the gap, so that the liquid can block the gap between the cover glass 2 and the carrier plate 11, thereby making the water in the bearing hole 13 evaporate slowly through the gap between the cover glass 2 and the carrier plate 11. Preferably, a hydrophobic coating is provided in the gap. After the cover glass 2 is placed on the carrier plate 11, the edge of the cover glass 2 is against the hydrophobic coating. The hydrophobic coating has extremely high surface tension and can repel water and other liquids. The hydrophobic coating can block the gap between the cover glass 2 and the carrier plate 11, thereby slowing down the volatilization speed of the water in the bearing hole 13 through the gap between the cover glass 2 and the carrier plate 11, and at the same time can prevent the sample in the bearing hole 13 from being contaminated by the outside, thereby ensuring the accuracy of the detection and the reusability of the sample.

[0027] The carrier plate 11 is provided with a plurality of bearing holes 13, and the bearing holes 13 are used to accommodate the objects to be detected. The radial cross-section of the bearing hole 13 is circular. Depending on the different objects to be detected, the radial cross-section of the bearing hole 13 can also be set to be rectangular, elliptical, triangular, etc. The central axis of the bearing hole 13 is perpendicular to the plane where the base plate 1 is located, so that the bearing hole 13 is set vertically. The plurality of bearing holes 13 are arranged in an equidistant array. Specifically, the diameters of the plurality of bearing holes 13 are equal and the overall array is rectangular. The bearing holes 13 are arranged along the edge of the carrier plate 11. The center distance between adjacent bearing holes 13 is 1.5-2.5mm, the diameter of the bearing hole 13 is 1-1.5mm, and the depth of the bearing hole 13 is 0.09-0.11mm. Each bearing hole 13 is used to carry an independent object to be detected, thereby maximizing the use of the area of the carrier plate 11 while ensuring the independence of each bearing hole 13, preventing cross-infection of multiple bearing holes 13, and realizing the simultaneous detection of multiple objects to be detected. The bearing hole 13 can be set to different diameters and depths according to the size of different objects to be detected.

[0028] In another embodiment, multiple bearing holes 13 are arranged along at least one direction, and the diameters of the multiple bearing holes 13 gradually increase. Specifically, the diameters of the bearing holes 13 can gradually increase in the horizontal or vertical direction, so that objects to be detected of different sizes can be detected on a single detection slide.

[0029] In another embodiment, the thickness of the carrier plate 11 is smaller than the depth of the groove 12, so that there is a height difference between the vertical distance between the top of the carrier plate 11 and the top of the base plate 1. After the cover glass 2 is placed on the carrier plate 11, the edge of the cover glass 2 can be against the side wall of the groove 12, thereby preventing the cover glass 2 from falling out of the groove 12.

[0030] Reference Figure 2 As shown, multiple markings are provided along the edge of the carrier plate 11 on the base plate 1. The markings oriented in the first direction are numerical markings 14, while the markings oriented in the second direction are alphabetical markings 15. Specifically, in this embodiment, the base plate 1 is provided with numerical markings 14 in the horizontal direction and alphabetical markings 15 in the vertical direction, thereby preventing confusion between horizontal and vertical recordings. When using the test slides in a laser scanning microscope, experimental condition names corresponding to row and column numbers are set in the automated sample loading machine and the microscope's automated stage software based on the experimental conditions. This allows for automated matching of experimental results with the conditions and avoids human error.

[0031] During use, the objects to be detected are placed in the carrying holes 13 respectively, and the detection liquid is injected into the carrying holes 13. At the same time, liquid is injected into the gap between the carrying plate 11 and the side wall of the groove 12 or a hydrophobic coating is applied. Finally, a cover glass 2 is placed on the carrying plate 11, and the edge of the cover glass 2 fits with the liquid or hydrophobic coating. After completion, the objects to be detected can be observed and detected through a microscope.

[0032] Example 2

[0033] Reference Figure 3 As shown, the utility model also provides a glass slide assembly, including the detection slide of embodiment 1, and also including a cover glass 2, the cover glass 2 is made of transparent borosilicate glass, the cover glass 2 is located on the supporting plate 11, and the edge of the cover glass 2 corresponds to the gap between the supporting plate 11 and the side wall of the groove 12.

[0034] Example 3

[0035] The present utility model also provides a microscope, comprising the glass slide assembly in the second embodiment.

[0036] The utility model provides a glass slide, a glass slide assembly and a microscope for detection, wherein the cover glass 2 is fitted with the liquid in the gap, so that the liquid can block the gap between the cover glass 2 and the carrier plate 11, thereby causing the moisture in the carrier hole 13 to evaporate slowly through the gap between the cover glass 2 and the carrier plate 11. At the same time, through the setting of the carrier hole 13, more detection liquid can be injected into a single detection position, the object to be detected in the detection hole can also be kept moist for a long time, and the volatilization rate of the moisture in the carrier hole 13 can be slowed down, thereby realizing high-throughput, automated and high-resolution detection of biological tissue samples.

[0037] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A slide glass for detection, characterized in that: include: a bottom plate, wherein the bottom plate is provided with a groove; A carrying plate is located in the groove, a gap exists between the edge of the carrying plate and the side wall of the groove, and a plurality of carrying holes are provided on the carrying plate, and the carrying holes are used to accommodate the object to be detected.

2. The detection slide according to claim 1, wherein: The center line of the bearing hole is perpendicular to the plane where the bottom plate is located.

3. The detection slide according to claim 1, wherein: A hydrophobic coating is provided in the gap between the carrying plate and the side wall of the groove.

4. The detection slide according to claim 1, wherein: The plurality of bearing holes are arranged in an equidistant array, the center distance between adjacent bearing holes is 1.5-2.5 mm, the diameter of the bearing hole is 1-1.5 mm, and the depth of the bearing hole is 0.09-0.11 mm.

5. The detection slide according to claim 4, characterized in that: A plurality of markings are provided on the bottom plate along the edge of the carrying plate, wherein the plurality of markings facing the first direction are digital markings, and the plurality of markings facing the second direction are letter markings.

6. The detection slide according to claim 1, wherein: The thickness of the carrying plate is smaller than the depth of the groove.

7. The detection slide according to claim 1, wherein: The plurality of bearing holes are arranged along at least one direction, and the diameters of the plurality of bearing holes gradually increase.

8. A slide assembly comprising the detection slide according to any one of claims 1 to 7, and further comprising a cover glass.

9. The slide assembly according to claim 8, wherein: The cover glass is located on the carrying plate.

10. A microscope comprising the slide assembly according to claim 8.

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