Culture system for butyric acid bacteria in white wine pit mud

Abstract

The utility model relates to microorganism culture, and provides a culture system for butyric acid bacteria in white wine pit mud, which comprises a feeding device, a plurality of culture devices connected in sequence, and a connecting device for connecting adjacent culture devices, the feeding device is connected with the uppermost culture device; the feeding device comprises a material mixing tank, a first stirring device arranged in the material mixing tank, a transfer tank, a negative pressure device connected with the transfer tank, a first guide pipe for connecting the transfer tank and the material mixing tank, a first valve arranged on the first guide pipe, a second guide pipe for connecting the transfer tank and the uppermost culture device, and a second valve arranged on the second guide pipe. According to the culture system for the butyric acid bacteria in the white wine pit mud, the butyric acid bacteria in the pit mud can be efficiently and automatically cultured.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial cultivation, and in particular to a cultivation system for butyric acid bacteria in liquor cellar mud. Background Art

[0002] Cellar mud is also known as a living national treasure. Every gram of ancient cellar mud contains hundreds of species and about one billion microorganisms involved in liquor brewing. It is called "microbial gold" by the scientific community.

[0003] In many winemaking processes, cellar mud is used to cultivate microorganisms. Since cellar mud contains many types of microorganisms, the required microorganisms, such as butyric acid bacteria, can be selectively screened out by adjusting the type of culture medium and various microenvironmental growth environments.

[0004] Most existing systems for cultivating microorganisms using cellar mud first use water to dissolve and dilute the mud into a fluid state, and then add it to the culture medium for cultivation. This entire process is largely manual, and the amount of mud and mud liquid added is not very precise, and there is also the risk of contamination of the mud. Therefore, a more efficient, high-precision, and automated cultivation system is needed. Utility Model Content

[0005] The purpose of the utility model is to provide a culture system for butyric acid bacteria in liquor cellar mud, which can efficiently and automatically culture butyric acid bacteria in the cellar mud.

[0006] The embodiments of the present invention are realized through the following technical solutions: the cultivation system of butyric acid bacteria in white wine cellar mud of the present invention includes a feeding device, a plurality of cultivation devices connected in sequence, and a connecting device for connecting adjacent cultivation devices; the feeding device is connected to the cultivation device at the most upstream; the feeding device includes a mixing tank, a first stirring device arranged in the mixing tank, a transfer tank, a negative pressure device connected to the transfer tank, a first conduit for connecting the transfer tank and the mixing tank, a first valve arranged on the first conduit, a second conduit for connecting the transfer tank and the cultivation device at the most upstream, and a second valve arranged on the second conduit.

[0007] Furthermore, the culture device includes an incubator; the feeding device also includes a first pump body arranged above the upstream incubator, a liquid inlet pipe arranged at the liquid inlet end of the first pump body, and a liquid outlet pipe arranged at the liquid outlet end of the first pump body; the second conduit is connected to the side wall of the liquid inlet pipe; the lower ends of the liquid inlet pipe and the liquid outlet pipe are both arranged in the incubator.

[0008] Furthermore, the height of the lower end of the liquid inlet pipe is higher than the height of the lower end of the liquid outlet pipe.

[0009] Furthermore, the feeding device also includes a water storage tank, a third conduit for connecting the water storage tank and the transfer tank, and a third valve provided on the third conduit.

[0010] Furthermore, an air intake pipe is provided on the side wall of the second conduit, and a fourth valve is provided on the air intake pipe; and one end of the air intake pipe away from the second conduit is arranged upward.

[0011] Furthermore, a liquid level detection device is provided in the transfer tank.

[0012] Furthermore, the lower end of the transfer tank is tapered, and the second conduit is connected to the lower end of the transfer tank.

[0013] Furthermore, the first stirring device includes a stirring shaft arranged in the mixing tank, a stirring blade arranged on the stirring shaft, and a motor for driving the stirring shaft to rotate.

[0014] Furthermore, the culture device also includes a second stirring device arranged in the culture box; and a feeding pipe arranged at the upper end of the culture box.

[0015] Furthermore, the connecting device includes a fourth conduit for connecting the adjacent incubator, and a second pump body provided on the fourth conduit; the negative pressure device includes a negative pressure machine, and a negative pressure pipe connected to the negative pressure machine; the negative pressure pipe is connected to the upper end of the transfer tank.

[0016] The technical solution of the embodiment of the utility model has at least the following advantages and beneficial effects: the cultivation system of butyric acid bacteria in white wine cellar mud of the utility model, when in use, the cellar mud and water are sent to the mixing tank in proportion, the cellar mud is diluted into a flowable state in the mixing tank, and then a negative pressure device is used to generate negative pressure in the transfer tank, and the cellar mud in the mixing tank is sucked into the transfer tank through the negative pressure and the first conduit (at this time, the first valve is opened and the second valve is closed), and then the first valve is closed, the second valve is opened, the negative pressure machine reduces the power or stops working, and the cellar mud in the transfer tank flows into the cultivation device through the second conduit for cultivation (the cultivation device is pre-loaded with liquid culture medium), and after cultivation in one cultivation device for a certain period of time, the culture solution is transferred to the downstream cultivation device, and the operation is carried out in sequence. After all the cultivation is completed, the culture solution is discharged and stored, so that the cellar mud can be efficiently and quantitatively put into the cultivation device for precise and quantitative cultivation, and multiple cultivation devices can be cultured continuously, and the corresponding culture medium can be put in advance according to the growth status of the bacteria, thereby improving production efficiency and the degree of automation. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0018] Figure 1 A schematic diagram of the structure of a butyric acid bacteria cultivation system in liquor cellar mud provided by an embodiment of the present invention;

[0019] Figure 2 This is a schematic structural diagram of the feeding device part provided in an embodiment of the present utility model.

[0020] Icons: 11-transfer tank, 12-mixing tank, 13-stirring blade, 14-first conduit, 15-first valve, 16-third conduit, 17-third valve, 18-water tank, 19-second conduit, 110-second valve, 111-air inlet pipe, 112-fourth valve, 113-negative pressure machine, 114-negative pressure pipe, 115-liquid inlet pipe, 116-liquid outlet pipe, 117-first pump body, 21-incubator, 22-feed pipe, 23-second stirring device, 24-fourth conduit, 25-second pump body. DETAILED DESCRIPTION

[0021] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings herein can be arranged and designed in various different configurations.

[0022] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but rather merely represents selected embodiments of the present invention. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort are also within the scope of protection of the present invention.

[0023] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0024] In the description of the present invention, it should be noted that if the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside" and the like appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the accompanying drawings, or is the orientation or position relationship in which the product of the application is usually placed when in use. It is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention.

[0025] It should also be noted that, in the description of this utility model, unless otherwise expressly specified or limited, the terms "disposed," "installed," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal connections between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0026] Example

[0027] The following is further described in conjunction with specific embodiments. Figure 1 -Attached Figure 2As shown, the culture system of butyric acid bacteria in white wine cellar mud of this embodiment includes a feeding device, a plurality of culture devices connected in sequence, and a connecting device for connecting adjacent culture devices; the feeding device is connected to the upstream culture device; the feeding device includes a mixing tank 12, a first stirring device arranged in the mixing tank 12, a transfer tank 11, a negative pressure device connected to the transfer tank 11, a first conduit 14 for connecting the transfer tank 11 and the mixing tank 12, a first valve 15 provided on the first conduit 14, a second conduit 19 for connecting the transfer tank 11 and the upstream culture device, and a second valve 110 provided on the second conduit 19. Specifically, during use, the cellar mud and water are fed into the mixing tank 12 in proportion, the cellar mud is diluted into a flowable state in the mixing tank 12, and then a negative pressure device is used to generate negative pressure in the transfer tank 11. The cellar mud in the mixing tank 12 is sucked into the transfer tank 11 through the negative pressure and the first conduit 14 (at this time, the first valve 15 is opened and the second valve 110 is closed). Then, the first valve 15 is closed, the second valve 110 is opened, and the negative pressure machine 113 reduces power or stops working. The cellar mud in the transfer tank 11 flows into the culture device through the second conduit 19 for culture (the culture device is pre-loaded with liquid culture medium). After culture in one culture device for a certain period of time, all the culture liquid is transferred to the downstream culture device. The operation is carried out in sequence. After all the culture is completed, the culture liquid is discharged and stored. In this way, the cellar mud can be efficiently and quantitatively added to the culture device for precise and quantitative culture, and multiple culture devices can be cultured continuously. The corresponding culture medium can be added in advance according to the growth status of the strain, thereby improving production efficiency and the degree of automation.

[0028] The culture device in this embodiment includes an incubator 21; the feeding device also includes a first pump body 117 located above the upstreammost incubator 21, a liquid inlet pipe 115 located at the liquid inlet end of the first pump body 117, and a liquid outlet pipe 116 located at the liquid outlet end of the first pump body 117; a second conduit 19 is connected to the side wall of the liquid inlet pipe 115; and the lower ends of the liquid inlet pipe 115 and the liquid outlet pipe 116 are both located within the incubator 21. Specifically, after the cellar mud is discharged from the second conduit 19, it enters the liquid inlet pipe 115. While the first pump body 117 draws the culture fluid from the incubator 21 through the liquid inlet pipe 115, it also draws the cellar mud through the second conduit 19 and returns it to the incubator 21 through the liquid outlet pipe 116. This ensures that the cellar mud is better dispersed in the culture fluid in the incubator 21.

[0029] In this embodiment, the height of the lower end of the liquid inlet pipe 115 is higher than the height of the lower end of the liquid outlet pipe 116. Specifically, when the first pump body 117 is in operation, the culture solution and the pit mud can be mixed in the horizontal direction, that is, laminar flow is generated.

[0030] The feeding device in this embodiment also includes a water storage tank, a third conduit 16 for connecting the water storage tank to the transfer tank 11, and a third valve 17 disposed on the third conduit 16. Specifically, since a single fermentation cycle takes a long time, to prevent microorganisms in the pit mud from fermenting in the transfer tank 11, after draining the pit mud from the transfer tank 11, a negative pressure device can be used to draw water from the water storage tank (in this case, the third valve 17 is opened, and the first valve 15 and the second valve 110 are closed). This water is then flushed against the inner wall of the transfer tank 11 and discharged through the second conduit 19 into the incubator 21. It is important to note that the culture solution concentration must be pre-adjusted so that, after the water from the water storage tank is fed into the incubator 21, the culture solution concentration reaches the specified value.

[0031] In this embodiment, an air intake pipe 111 is provided on the sidewall of the second conduit 19, and a fourth valve 112 is provided on the air intake pipe 111. The end of the air intake pipe 111, which is away from the second conduit 19, faces upward. Specifically, after water is drawn from the water storage tank through the third conduit 16, air is drawn in through the air intake pipe 111 to agitate the liquid in the transfer tank 11, thereby removing any mud adsorbed on the walls of the transfer tank 11. The fourth valve 112 can also be a one-way valve, air pressure valve, or other structure.

[0032] In this embodiment, a liquid level detection device is provided in the transfer tank 11. Specifically, the use of the liquid level detection device can better control the amount of sucked pit mud and water, wherein the liquid level detection device can use common structures such as infrared type and float rod.

[0033] The lower end of the transfer tank 11 in this embodiment is tapered, and the second conduit 19 is connected to the lower end of the transfer tank 11. Specifically, this can more fully and quickly drain the liquid in the transfer tank 11.

[0034] The first stirring device in this embodiment includes a stirring shaft disposed in the mixing tank 12, a stirring blade 13 disposed on the stirring shaft, and a motor for driving the stirring shaft to rotate.

[0035] The culture device in this embodiment further includes a second stirring device 23 disposed in the culture box 21 ; and a feeding pipe 22 disposed at the upper end of the culture box 21 .

[0036] In this embodiment, the connection device includes a fourth conduit 24 for connecting adjacent incubators 21, and a second pump 25 mounted on the fourth conduit 24. The negative pressure device includes a negative pressure machine 113 and a negative pressure pipe 114 connected to the negative pressure machine 113. The negative pressure pipe 114 communicates with the upper end of the transfer tank 11. Specifically, the fourth conduit 24 and the second pump 25 are primarily used to transfer the fermented material from the upstream incubator 21 to the downstream incubator 21 for further cultivation. The culture medium formula can be finely adjusted based on the status of the bacterial colonies and is then delivered to the incubator 21 via the feed pipe 22.

[0037] In summary, the cultivation system of butyric acid bacteria in white wine cellar mud of this embodiment is as follows: when in use, the cellar mud and water are fed into the mixing tank 12 in proportion, the cellar mud is diluted into a flowable state in the mixing tank 12, and then a negative pressure device is used to generate negative pressure in the transfer tank 11. The cellar mud in the mixing tank 12 is sucked into the transfer tank 11 through the negative pressure and the first conduit 14 (at this time, the first valve 15 is opened and the second valve 110 is closed). Then, the first valve 15 is closed, the second valve 110 is opened, the negative pressure machine 113 reduces the power or stops working, and the cellar mud in the transfer tank 11 flows into the cultivation device through the second conduit 19 for cultivation (the cultivation device is pre-loaded with liquid culture medium). After cultivation in one cultivation device for a certain period of time, the culture solution is transferred to the downstream cultivation device. The operation is carried out in sequence. After all the cultivation is completed, the culture solution is discharged and stored. In this way, the cellar mud can be efficiently and quantitatively added to the cultivation device for precise and quantitative cultivation, and multiple cultivation devices can be cultured continuously. The corresponding culture medium is added in advance according to the growth status of the bacteria, thereby improving production efficiency and the degree of automation.

[0038] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A culture system for butyric acid bacteria in liquor cellar mud, characterized by: It comprises a feeding device, a plurality of culture devices connected in sequence, and a connecting device for connecting adjacent culture devices; the feeding device is connected to the culture device at the most upstream; The feeding device includes a mixing tank (12), a first stirring device provided in the mixing tank (12), a transfer tank (11), a negative pressure device connected to the transfer tank (11), a first conduit (14) for connecting the transfer tank (11) and the mixing tank (12), a first valve (15) provided on the first conduit (14), a second conduit (19) for connecting the transfer tank (11) and the culture device at the most upstream, and a second valve (110) provided on the second conduit (19); The culture device includes a culture box (21); the feeding device also includes a first pump body (117) arranged above the culture box (21) at the most upstream end, a liquid inlet pipe (115) arranged at the liquid inlet end of the first pump body (117), and a liquid outlet pipe (116) arranged at the liquid outlet end of the first pump body (117); The second conduit (19) is in communication with the side wall of the liquid inlet pipe (115); the lower ends of the liquid inlet pipe (115) and the liquid outlet pipe (116) are both arranged in the incubator (21).

2. The culture system for butyric acid bacteria in liquor pit mud according to claim 1, characterized in that: The height of the lower end of the liquid inlet pipe (115) is higher than the height of the lower end of the liquid outlet pipe (116).

3. The culture system for butyric acid bacteria in liquor pit mud according to claim 1, characterized in that: The feeding device further comprises a water storage tank, a third conduit (16) for connecting the water storage tank and the transfer tank (11), and a third valve (17) provided on the third conduit (16).

4. The culture system for butyric acid bacteria in liquor pit mud according to claim 3, characterized in that: An air intake pipe (111) is provided on the side wall of the second conduit (19), and a fourth valve (112) is provided on the air intake pipe (111); an end of the air intake pipe (111) away from the second conduit (19) is arranged upward.

5. The culture system for butyric acid bacteria in liquor pit mud according to claim 1, characterized in that: A liquid level detection device is provided in the transfer tank (11).

6. The culture system for butyric acid bacteria in liquor pit mud according to claim 1, characterized in that: The lower end of the transfer tank (11) is tapered, and the second conduit (19) is communicated with the lower end of the transfer tank (11).

7. The culture system for butyric acid bacteria in liquor pit mud according to claim 1, characterized in that: The first stirring device comprises a stirring shaft arranged in the mixing tank (12), a stirring blade (13) arranged on the stirring shaft, and a motor for driving the stirring shaft to rotate.

8. The culture system for butyric acid bacteria in liquor pit mud according to claim 1, characterized in that: The culture device further comprises a second stirring device (23) arranged in the culture box (21); and a feeding pipe (22) arranged at the upper end of the culture box (21).

9. The culture system for butyric acid bacteria in liquor pit mud according to claim 1, characterized in that: The connecting device comprises a fourth conduit (24) for connecting the adjacent incubator (21), and a second pump body (25) provided on the fourth conduit (24); The negative pressure device comprises a negative pressure machine (113) and a negative pressure pipe (114) connected to the negative pressure machine (113); the negative pressure pipe (114) is in communication with the upper end of the transfer tank (11).

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