Microorganism rapid expanding culture device for aquaculture

Abstract

The utility model discloses a rapid microorganism expanding culture device for aquaculture, relates to the field of fermentation, and aims to solve the problems that a stainless steel fermentation tank system is complicated in structure, large in occupied area, higher in price, heavier in weight, inconvenient to carry and required to be operated by professional technicians, and an aeration disc of a conventional fermentation tank is easy to block. According to the fermentation tank disclosed by the utility model, the aeration disc / aeration beads are arranged, so that a stirring effect is achieved, dissolved oxygen can be increased, and microorganism culture is facilitated. Therefore, the double effects of stirring and increasing dissolved oxygen are achieved by arranging the aerators. The air compressor is communicated with the air inlet pipe to ventilate the fermentation tank. The heating pipe controls the temperature through the control box to achieve the effects of culture medium disinfection and temperature control in the expanding culture process. The S-shaped exhaust pipe can effectively exhaust air and prevent infectious microbes in external air from entering the tank to cause pollution at the same time. The weight of the fermentation tank is reduced. And the input cost is reduced.

Description

Technical Field

[0001] The utility model belongs to the field of fermentation, and in particular relates to a microorganism rapid expansion and cultivation device for aquaculture. Background Art

[0002] Microbial fermentation is a type of biological culture. The microorganisms cultured primarily include viruses, bacteria, actinomycetes, and fungi. Microbial fermentation involves preparing specific culture media based on the specific microorganisms and their living habits, and then cultivating them in incubators or fermentation tanks.

[0003] Common fermentation tanks can be divided into the following types according to their materials

[0004] 1. Ordinary fermentation tank

[0005] Conventional fermentation tanks are typically made of plastic. Their principle is to use vents in the tank to control the flow of oxygen inside, thereby promoting microbial fermentation. These fermentation tanks are primarily suitable for fermenting small quantities of food. They are relatively inexpensive and easy to use. However, their disadvantages include being heat-resistant, easily damaged, and difficult to clean.

[0006] 2. Glass Fermentation Tank

[0007] Glass fermentation tanks are made of a transparent material. Their principle is to control the flow of oxygen inside the tank through the vent holes on the tank body, promoting the fermentation of microorganisms. At the same time, because the bottom of the glass tank can project light, the fermentation process can be observed very clearly. Disadvantages: Glass material is easy to break and is relatively expensive.

[0008] 3. Ceramic Fermentation Tank

[0009] Ceramic fermentation tanks are specialized tanks fired at high temperatures. They rely on microbial fermentation to thrive on their surface, and the microorganisms produced by the tank itself contribute to improved fermentation quality. These tanks are generally heavy, requiring special attention to their stability during use. The tanks harbor microorganisms, are heavy, and require considerable effort to transport, necessitating a high investment.

[0010] 4. Stainless steel fermentation tank

[0011] Stainless steel fermentation tanks are rigid, non-deformable fermentation equipment made of stainless steel. Their principle is to utilize a specially treated steel surface and designed vents to regulate oxygen flow within the tank, promoting fermentation. This provides excellent rigidity, resistance to deformation, and ease of cleaning. However, stainless steel fermentation tank systems, consisting of a steam boiler, air compressor, air freeze dryer, and propagation tanks, are complex, require a large floor space, are expensive, heavy, and difficult to transport.

[0012] In addition to factors such as structure, volume and cost, the above-mentioned fermentation tanks also require professional fermentation technicians to operate, and are not suitable for aquaculture farmers to ferment bacteria on-site for aquaculture. Utility Model Content

[0013] The utility model aims to solve the problems that stainless steel fermentation tank systems have complex structures, large floor space, high prices, heavy weight, are inconvenient to carry, require professional technicians to operate, and the aeration plates of current fermentation tanks are easily clogged. The utility model proposes a rapid microbial expansion device for aquaculture.

[0014] The utility model discloses a rapid microbial expansion and cultivation device for aquaculture, comprising a fermentation tank, an aeration plate, a heating pipe, an air inlet pipe, an inoculation port, a feed port, a manhole and a discharge port;

[0015] The aeration plate is located at the bottom of the fermentation tank, the air inlet of the aeration plate is connected to the air outlet of the air inlet pipe, and the air inlet of the air inlet pipe is arranged outside the fermentation tank; a discharge port is provided at the bottom of the fermentation tank, a feed port and a manhole are respectively provided at the top, and a heating pipe is provided inside the fermentation tank.

[0016] The utility model has the following beneficial effects:

[0017] The present invention is different from existing fermentation tanks, especially stainless steel fermentation tanks. The fermentation tank of the present invention does not adopt a stirring mechanism, but plays a stirring role through the aeration plate / aeration beads provided, and can increase dissolved oxygen to be more conducive to microbial culture. Therefore, the present invention achieves the dual effects of stirring and increasing dissolved oxygen by providing an aerator. The air compressor is connected to the air inlet pipe to ventilate the fermentation tank. The heating pipe controls the temperature through the control box to achieve the purpose of sterilizing the culture medium and controlling the temperature during the expansion culture process. The "S"-shaped exhaust pipe can achieve effective exhaust while preventing miscellaneous bacteria in the external air from entering the tank and causing contamination.

[0018] The gas blown out from the transverse air outlets on both sides of the vertical air outlet of the aeration plate of the present invention can continuously sweep away the microbial fermentation products scattered on the vertical air outlet, such as residues, flocs, etc., thereby reducing the probability of blockage.

[0019] This utility model replaces the stirring mechanism with an aeration disc / aeration bead, reducing the weight of the fermentation tank. Heating tubes replace high-pressure steam sterilization and temperature control, reducing investment costs. The accompanying fermentation process is easy to operate, and microbial growth is rapid, making it convenient for farmers to use for on-site fermentation. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the structure of the expansion device of the utility model;

[0021] Figure 2 This is a top view of the expansion device of the utility model;

[0022] Figure 3 This is a schematic diagram of the aeration plate of the utility model;

[0023] In the figure, an aerator 1, a heat pipe 2, an air inlet pipe 3, an inoculation port 4 (the liquid level gauge is eliminated), a temperature probe 5, a manhole 6, a feed port 7, a discharge port 8, an exhaust pipe 9, a fermentation tank 10, an air compressor 11, a vertical air outlet 12, and a horizontal air outlet 13. DETAILED DESCRIPTION

[0024] In order to make the objectives, technical solutions and advantages of the embodiments of the present invention more clearly understood, the spirit of the contents disclosed in the present invention will be described in detail below. After understanding the embodiments of the contents of the present invention, any technician in the relevant technical field can change and modify the contents of the present invention based on the techniques taught by the contents of the present invention without departing from the spirit and scope of the contents of the present invention.

[0025] The exemplary embodiments of the present invention and the description thereof are used to explain the present invention but are not intended to limit the present invention.

[0026] Specific implementation method 1: Combination Figures 1 to 3 This embodiment describes a device for rapid microbial expansion in aquaculture, comprising a fermentation tank 10, an aeration plate 1, a heating pipe 2, an air inlet 3, an inoculation port 4, a feed port 7, a manhole 6, and a discharge port 8.

[0027] The aeration plate 1 is located at the bottom of the fermentation tank 10, the air inlet of the aeration plate 1 is connected to the air outlet of the air inlet pipe 3, and the air inlet of the air inlet pipe 3 is arranged outside the fermentation tank 10; the fermentation tank 10 is provided with a discharge port 8 at the bottom, and a feed port 7 and a manhole 6 at the top, respectively, and a heating pipe 2 is provided inside the fermentation tank 10.

[0028] Specific implementation method 2: Combination Figures 1 to 3 This embodiment is described. The difference between this embodiment and the first embodiment is that the air outlet of the aeration plate 1 is composed of a vertical air outlet 12 and a horizontal air outlet 13 ; and a horizontal air outlet 13 is provided between two adjacent vertical air outlets 12 .

[0029] The rest is the same as the first embodiment.

[0030] Specific implementation method three: Combination Figures 1 to 3 This embodiment is described. The difference between this embodiment and the first embodiment is that there are two transverse air outlets 13 arranged opposite to each other.

[0031] The rest is the same as the first embodiment.

[0032] Specific implementation method four: Combination Figures 1 to 3This embodiment is described. The difference between this embodiment and the first embodiment is that an air inlet pipe 3 is provided in the fermentation tank 10 .

[0033] The rest is the same as the first specific implementation method.

[0034] Specific implementation method five: Combination Figures 1 to 3 This embodiment is described. The difference between this embodiment and the first embodiment is that an inoculation port 4 is provided in the fermentation tank 10 .

[0035] The rest is the same as the first specific implementation method.

[0036] Specific implementation method six: combination Figures 1 to 3 This embodiment is described. The difference between this embodiment and the first embodiment is that a temperature probe 5 is provided in the fermentation tank 10 .

[0037] The rest is the same as the first embodiment.

[0038] Specific implementation method seven: combination Figures 1 to 3 This embodiment is described. The difference between this embodiment and the first embodiment is that an exhaust pipe 9 is provided on the top of the fermentation tank 10 .

[0039] The rest is the same as the first embodiment.

[0040] Specific implementation method eight: combination Figures 1 to 3 This embodiment is described. The difference between this embodiment and the first embodiment is that an exhaust pipe 9 is provided on the top of the fermentation tank 10. The exhaust pipe 9 is "S"-shaped and consists of two semicircles.

[0041] Other components and connection methods are the same as those in the first embodiment.

[0042] Specific implementation method nine: Combination Figures 1 to 3 This embodiment is described. The difference between this embodiment and the first embodiment is that the heating tube 2 is electrically connected to the temperature control box.

[0043] The rest is the same as the first embodiment.

[0044] Specific implementation method ten: Combination Figures 1 to 3 This embodiment is described. The difference between this embodiment and the first embodiment is that the air inlet of the air inlet pipe 3 is connected to the air compressor 11.

[0045] Other components and connection methods are the same as those in the first embodiment.

[0046] Example

[0047] 1. Expanding the culture of bacteria

[0048] Bacillus subtilis subsp. Steinernema, Company No.: B30

[0049] 2. Expansion Culture Medium

[0050] Sodium acetate 5g / L, calcium carbonate 1g / L, potassium chloride 3g / L, soybean meal 4g / L, glucose monohydrate 3g / L, corn flour 2g / L.

[0051] 3. Expansion technology (taking 400 kg expansion as an example)

[0052] 3.1 Rinse the fermentation tank 10 with clean tap water and drain the washing water.

[0053] 3.2 Add 100 kg of clean tap water (enough to cover heating tube 2).

[0054] 3.3 Prepare 400 kg of culture medium according to the "Expansion Culture Medium" and add it to the fermentation tank 10.

[0055] 3.4 Turn on the air compressor 11 and stir through the aerator 1.

[0056] 3.5 Turn on the heating switch and control the heating tube 2 to heat to 100℃ through the control box (do not stop the gas in the middle).

[0057] 3.6 Maintain 100℃ for 10 minutes and then turn off the heating switch.

[0058] 3.7 After turning off the heating switch, continue to ventilate and cool down. When the temperature drops to 70℃, add 300 kg of clean tap water.

[0059] 3.8 If the culture medium temperature is between 41-45°C, add 0.8 kg of B30 liquid seed (cell count 20 billion / ml). Preferably, inoculate 0.8 kg of B30 liquid seed at 41°C and expand the culture for about 6-8 hours until the cell count reaches a maximum, estimated at 1.5 billion / ml. Inoculate 0.8 kg of B30 liquid seed at 45°C and the cell count reaches a maximum after 12 hours. After 18 hours, the cell count reaches 750 million / ml, with about 20% of the cells forming spores.

[0060] 3.9 After inoculation, the air compressor 11 is turned on to aerate the aerator 1 continuously without maintaining the temperature and the natural pH value (the pH value after inoculation is basically in the range of 6.6-7.4) until the expansion cultivation is completed or the seed is used up.

Claims

1. A microbial rapid culture device for aquaculture, characterized in that It includes a fermentation tank (10), an aeration plate (1), Heating pipe (2), air inlet pipe (3), inoculation port (4), feed port (7), manhole (6) and discharge port (8); The aeration plate (1) is located at the bottom of the fermentation tank (10), the air inlet of the aeration plate (1) is connected to the air outlet of the air inlet pipe (3), and the air inlet of the air inlet pipe (3) is arranged outside the fermentation tank (10); the bottom of the fermentation tank (10) is provided with a discharge port (8), the top is provided with a feed port (7) and a manhole (6), and the fermentation tank (10) is provided with a heating pipe (2).

2. A microbial rapid expansion device for aquaculture according to claim 1, characterized in that The air outlet of the aeration plate (1) is composed of a vertical air outlet (12) and a horizontal air outlet (13); a horizontal air outlet (13) is provided between two adjacent vertical air outlets (12).

3. A microbial rapid expansion device for aquaculture according to claim 2, characterized in that There are two transverse air outlets (13) which are arranged opposite to each other.

4. The microbial rapid expansion device for aquaculture according to claim 1, characterized in that An air inlet pipe (3) is provided in the fermentation tank (10).

5. The microbial rapid expansion and cultivation device for aquaculture according to claim 1, characterized in that An inoculation port (4) is provided in the fermentation tank (10).

6. The microbial rapid expansion device for aquaculture according to claim 1, characterized in that A temperature probe (5) is provided in the fermentation tank (10).

7. The microbial rapid expansion device for aquaculture according to claim 1, characterized in that An exhaust pipe (9) is provided on the top of the fermentation tank (10).

8. The device for rapid microbial expansion and cultivation for aquaculture according to claim 7, characterized in that The exhaust pipe (9) is "S" shaped and consists of two semicircles.

9. The microbial rapid culture device for aquaculture according to claim 1, characterized in that The heating tube (2) is electrically connected to the temperature control box.

10. The microorganism rapid expansion and cultivation device for aquaculture according to claim 1, characterized in that The air inlet of the air inlet pipe (3) is in communication with the air compressor (11).

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