Cell counting plate

Abstract

The utility model provides a cell counting plate and belongs to the technical field of cell counting. Comprising a counting plate body and cover glass, a sample adding area and supporting areas located on the two sides of the sample adding area are arranged on the counting plate body, a counting groove is formed in the sample adding area, a grid area is arranged in the counting groove, limiting grooves are formed in the positions, located in the two supporting areas, of the counting plate body, and the sides, close to the sample adding area, of the limiting grooves are through; the counting groove is covered with the cover glass, the two ends of the cover glass are in lap joint in the limiting groove, and the limiting groove is provided with a movable gap for the two ends of the cover glass to move. When bubbles appear between the cover glass and the counting groove, the counting plate body is slightly inclined, the end, extending out of the cover glass, of the counting groove is inclined upwards, the two ends, in the limiting groove, of the cover glass are pressed, the cover glass is horizontally moved at multiple angles under the movement allowance provided by the limiting groove, and the gap between the counting groove and the cover glass can be filled with a cell solution; the cell counting plate has the effect of improving the test counting efficiency of the cell counting plate.

Description

Technical Field

[0001] The utility model relates to the technical field of cell counting, in particular to a cell counting plate. Background Art

[0002] In cell culture work, it is often necessary to understand the living status of cells and distinguish between live and dead cells, determine the cell inoculation concentration and quantity, and understand the cell survival rate and proliferation rate. Cell counting is generally performed using a hemocytometer.

[0003] The current cell counting chamber consists of a counting chamber body and a cover glass. The counting chamber body is divided into several large squares, with medium squares in the center and four corners of each large square. Each medium square is divided into multiple small squares for convenient counting. After the cover glass is placed on the counting chamber body, a 0.1mm high counting chamber is formed between the cover glass and the large squares on the counting chamber body. Cell culture medium is dripped into one side of the cover glass. After the cell culture medium is siphoned and fills the counting area, it is left to stand until the cells settle on the counting chamber body. The cells can then be counted and placed under a microscope for observation.

[0004] However, when the cell culture medium is siphoned between the coverslip and the counting plate under capillary action, improper sample loading can easily lead to bubbles between the coverslip and the counting plate. In order to avoid the height of the counting cavity between the coverslip and the counting plate being destroyed during the bubble removal process, thereby affecting the accuracy of the counting volume, the coverslip and the counting plate are usually cleaned again, and then the sample loading operation is performed again after both are dried, resulting in low counting efficiency. Utility Model Content

[0005] In view of the above problems, the utility model provides a cell counting plate.

[0006] In order to achieve the above-mentioned utility model purpose, the technical solution adopted by the utility model is as follows:

[0007] Provided is a cell counting chamber, comprising a counting chamber body and a cover glass. A support area and a sample loading area are provided on the counting chamber body along its length direction. Two support areas are provided, and the two support areas are respectively located on both sides of the sample loading area. A counting groove is provided in the sample loading area, and a grid area is provided in the counting groove. Limit grooves are provided in the two support areas of the counting chamber body, and the limit grooves are continuous on the side close to the sample loading area. The cover glass is covered on top of the counting groove, and the two ends of the cover glass are overlapped in the limit grooves. The limit grooves are provided with movable gaps for the two ends of the cover glass to move.

[0008] Furthermore, the counting slots are symmetrically arranged in pairs on the counting board body, and a plurality of pairs of counting slots are arranged at intervals along the length direction of the counting board body.

[0009] Furthermore, a support frame is provided on the outer side of the counting groove in the sample adding area, and the top wall of the support frame is flush with the inner bottom wall of the limiting groove.

[0010] Furthermore, a drainage groove is provided on the outside of each support frame in the sample adding area, and the multiple drainage grooves are interconnected. A discharge port for discharging liquid in the drainage groove is provided on the side wall of the counting plate body.

[0011] Furthermore, a cover glass extends from one side edge of the counting groove, and an overflow groove connected to the edge of the counting groove extending from the cover glass is provided on the edge of the counting plate body.

[0012] Furthermore, a drainage groove is provided in the counting groove along the circumference of the grid area.

[0013] Furthermore, both ends of the drainage groove are connected to the overflow groove, and the depth of the drainage groove gradually increases from the overflow groove to a direction away from the overflow groove.

[0014] The beneficial effects of the utility model are as follows: after the cover glass is covered on the counting plate body, the two ends of the cover glass are embedded and supported in the limiting grooves in the supporting areas on both sides of the counting plate body; after the cell solution is dripped into the counting groove on one side of the cover glass, the cells are sucked into the space between the cover glass and the counting groove under the capillary action; when bubbles appear between the cover glass and the counting groove, by slightly tilting the counting plate body so that the counting groove extends out of the end of the cover glass and tilts upward, and pressing the two ends of the cover glass in the limiting groove, the cover glass is moved horizontally at multiple angles within the movable margin provided by the limiting groove, so that the cell solution can fill the gap between the counting groove and the cover glass, the bubbles are discharged, and the cell coefficient efficiency is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Schematic diagram of the overall structure of the cell counting chamber according to an embodiment of the present application.

[0016] Figure 2 This is a schematic diagram of the overall structure of the counting board body of an embodiment of the present application.

[0017] Figure 3 This is a schematic diagram of the partial cross-sectional structure of the counting plate body at the drainage groove of an embodiment of the present application.

[0018] Among them, 1. Counting plate body; 11. Support area; 111. Limiting groove; 12. Sample adding area; 121. Counting groove; 122. Grid area; 123. Support frame; 124. Drainage groove; 125. Overflow groove; 126. Drainage groove; 2. Cover glass. DETAILED DESCRIPTION

[0019] In order to better understand the above technical solution, the above technical solution will be described in detail below with reference to the accompanying drawings and specific implementation methods.

[0020] The present application discloses a cell counting plate, referring to Figure 1 and Figure 2 , including a counting board body 1 and a cover glass 2. The counting board body 1 is provided with a support area 11, a sample adding area 12 and a support area 11 in sequence along its length direction. A counting groove 121 is provided in the sample adding area 12, and a grid area 122 is provided in the counting groove 121. A limiting groove 111 is provided in both support areas 11. The limiting groove 111 passes through one side of the counting groove 121. When the cover glass 2 is covered on the counting board body 1, the cover glass 2 spans the sample adding area 12, and the two ends of the cover glass 2 are respectively overlapped in the two limiting grooves 111. A counting cavity gap of 0.1 mm is left between the bottom wall of the cover glass 2 and the inner bottom wall of the counting groove 121, and a movable gap of 2-3 mm is left between the end of the cover glass 2 and the inner wall of the limiting groove 111. A grid area 122 is provided in the counting trough 121. A cover glass 2 extends from one side of the counting trough 121, so that the counting chamber is connected to the outside world. By dripping a cell solution into the counting trough 121 from which the cover glass 2 extends, the cell solution is siphoned into the counting chamber. When there are bubbles in the counting chamber, the experimenter holds the counting plate body 1, tilts the counting plate body 1 slightly, and faces the opening of the counting chamber where the bubbles are located upward. The experimenter places the index fingers of both hands on the bottom wall of the counting plate body 1, and presses the two ends of the cover glass 2 with the thumbs of both hands respectively. The cover glass 2 is moved at multiple angles in the limiting groove 111 by pressing the index fingers, so that the cell solution in the counting chamber gradually fills the counting chamber, and the bubbles in the counting chamber are discharged upward from the opening of the counting chamber, thereby avoiding the operation of cleaning the cell counting plate and re-adding samples, thereby improving the experimental counting efficiency. The cover glass 2 does not move and rub over a large range to avoid contamination of the cell solution. The cover glass 2 always remains in contact with the counting chamber body 1, so that the volume of the counting chamber does not change, the number of cells in the cell solution in the counting chamber is maintained, and the accuracy of the counting results is guaranteed.

[0021] In the embodiment of the present application, the counting slots 121 are symmetrically arranged in pairs on the counting plate body 1, and multiple pairs of counting slots 121 are provided at intervals along the length of the counting plate body 1. One or more cell solutions to be tested can be dripped into multiple counting slots 121 of the counting plate at the same time, forming multiple comparison samples, thereby improving the accuracy and efficiency of the test count.

[0022] Furthermore, a support frame 123 is provided outside the counting wells 121 within the sample loading area 12. The top wall of the support frame 123 is flush with the inner bottom wall of the retaining groove 111. The support frame 123 separates the counting wells 121, preventing mixing of cell solutions in different counting wells 121 during horizontal movement of the cover glass 2. Furthermore, the support frame 123 and the retaining groove 111 jointly support the cover glass 2, effectively maintaining the volume of the counting chamber and improving the accuracy of the test count.

[0023] In the embodiment of the present application, drainage grooves 124 are provided on the outer side of each support frame 123 within the sample loading area 12. The multiple drainage grooves 124 are interconnected, and outlets for discharging liquid from the drainage grooves 124 are provided on the sidewall of the counting chamber body 1. When a large amount of cell solution is dripped between the cover glass 2 and the counting chamber body 1, even if the cover glass 2 is lifted off the support frame 123 by liquid tension, the drainage grooves 124 can effectively isolate the various counting chambers 121, preventing the mixing of cell solutions in different counting chambers 121 and improving the accuracy of cell counting.

[0024] Furthermore, an overflow groove 125 is provided on the edge of the counting plate body 1 and is connected to the edge of the counting groove 121 extending out of the cover glass 2. The overflow groove 125 is an inclined slope. When too much cell solution is dripped into the counting groove 121, the excess cell solution of the cells outside the cover glass 2 is discharged downstream from the connection between the counting groove 121 and the overflow groove 125, thereby preventing the cell solution from overflowing to the upper surface of the cover glass 2 and interfering with the cell counting.

[0025] Reference Figure 3 To improve the efficiency of cell solution filling the counting chamber, in this embodiment of the present application, drainage grooves 126 are provided within the counting chamber 121 along the circumference of the grid area 122. Guided by drainage grooves 126, the cell solution can quickly infiltrate the inner side of the counting chamber 121, accelerating the efficiency of the cell solution filling the counting chamber. Drainage grooves 126 also provide a channel for air to escape from the counting chamber, reducing the occurrence of bubbles in the counting chamber.

[0026] Furthermore, both ends of the drainage groove 126 are connected to the overflow groove 125, and the depth of the drainage groove 126 gradually increases from the overflow groove 125 to the direction away from the overflow groove 125. The design of the drainage groove 126 with a gradually increasing cross-sectional area from the opening of the counting chamber formed by the cover glass 2 and the counting chamber 121 can gradually increase the siphon suction force of the drainage groove 126 on the cell solution, thereby improving the efficiency of the cell solution filling the counting chamber.

[0027] Those skilled in the art will appreciate that, although preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they become aware of the underlying inventive concepts. Therefore, the appended claims are intended to be interpreted as encompassing the preferred embodiments and all changes and modifications that fall within the scope of the present invention. Clearly, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, the present invention is intended to encompass such changes and modifications as fall within the scope of the claims and their equivalents.

Claims

1. A cell counting chamber, characterized in that: The counting board body (1) comprises a counting board body (1) and a cover glass (2). A support area (11) and a sample loading area (12) are provided on the counting board body (1) along its length direction. Two support areas (11) are provided, and the two support areas (11) are respectively located on both sides of the sample loading area (12). A counting groove (121) is provided in the sample loading area (12), and a grid area (122) is provided in the counting groove (121). A limiting groove (111) is provided in both supporting areas (11) on the counting board body (1). The limiting groove (111) is through-connected on one side close to the sample loading area (12). The cover glass (2) covers the top of the counting groove (121), and the two ends of the cover glass (2) are overlapped in the limiting groove (111). The limiting groove (111) is provided with a movable gap for the two ends of the cover glass (2) to move.

2. A cell counting chamber according to claim 1, characterized in that: The counting slots (121) are symmetrically arranged in pairs on the counting board body (1), and a plurality of pairs of the counting slots (121) are spaced apart along the length direction of the counting board body (1).

3. A cell counting chamber according to claim 2, characterized in that: A support frame (123) is provided outside the counting slot (121) in the sample adding area (12), and the top wall of the support frame (123) is flush with the inner bottom wall of the limiting slot (111).

4. A cell counting chamber according to claim 3, characterized in that: A drainage groove (124) is provided on the outside of each support frame (123) in the sample adding area (12), and a plurality of the drainage grooves (124) are interconnected. A discharge port for discharging liquid in the drainage groove (124) is provided on the side wall of the counting plate body (1).

5. A cell counting chamber according to claim 4, characterized in that: A cover glass (2) extends from one side edge of the counting groove (121), and an overflow groove (125) connected to the edge of the counting groove (121) extending from the cover glass (2) is provided on the edge of the counting plate body (1).

6. A cell counting chamber according to claim 5, characterized in that: A drainage groove (126) is provided in the counting groove (121) along the circumference of the grid area (122).

7. A cell counting chamber according to claim 6, characterized in that: Both ends of the drainage groove (126) are connected to the overflow groove (125), and the depth of the drainage groove (126) gradually increases from the overflow groove (125) to a direction away from the overflow groove (125).

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