Totally-closed separation type cell culture bottle

Abstract

The utility model relates to a totally-closed separation type cell culture bottle which comprises a culture bottle body, and the culture bottle body comprises a first containing cavity, a second containing cavity, a third containing cavity, a first communicating opening, a second communicating opening and a third communicating opening, a first sliding block, a second sliding block and a third sliding block which can slide are sequentially arranged on the first communication port, the second communication port and the third communication port; the culture bottle body is provided with a blocking part, a ventilation part and a containing part. By adjusting the position of the sliding block, each containing cavity can be controlled to be isolated from or communicated with external air, so that oxygen requirements in different stages in the cell culture process are met, and the situation that when a box cover on a culture bottle needs to be switched to meet the oxygen requirements of cells in different stages, a bottle cap on the culture bottle needs to be replaced is improved, so that the cost is reduced. The problems that replacement steps are tedious, and a box cover cannot be rapidly switched to meet oxygen requirements in different stages in the cell culture process exist in the prior art.

Description

Technical Field

[0001] The present application relates to the technical field of cell culture flasks, and in particular to a fully enclosed separation cell culture flask. Background Art

[0002] With the continuous advancement of biotechnology and medical research, cell culture technology has been widely used in basic research, drug screening, and tissue engineering. As an indispensable device in the cell culture process, the design and function of cell culture flasks have a significant impact on the culture results. In recent years, fully enclosed separation cell culture flasks have attracted the attention of researchers because their unique structural design can effectively improve the efficiency and quality of cell culture.

[0003] Among the relevant technical means, fully enclosed separate cell culture flasks can be used to achieve effective cell culture environment control and increase cell yield, which can reduce the risk of contamination and cross-infection, while better simulating the in vivo environment, helping to improve the efficiency and quality of cell culture.

[0004] Regarding the above technical solution, although the existing fully enclosed separate cell culture flask can effectively control the cell culture environment and increase cell yield, when the box cover on the culture flask needs to be switched to meet the oxygen demand of the cells at different stages, the bottle cover on the culture flask needs to be replaced. There are problems such as cumbersome replacement steps and the inability to quickly switch the box cover to meet the oxygen demand at different stages of the cell culture process. Utility Model Content

[0005] In order to improve the problem that when the box cover on the culture bottle needs to be switched to meet the oxygen demand of cells at different stages, the bottle cover on the culture bottle needs to be replaced, the replacement steps are cumbersome, and the box cover cannot be quickly switched to meet the oxygen demand at different stages of the cell culture process, the present application provides a fully enclosed separated cell culture bottle.

[0006] The present application provides a fully enclosed separated cell culture flask, comprising a culture flask body, wherein the culture flask body includes a first accommodating chamber, a second accommodating chamber and a third accommodating chamber, and the accommodating spaces of the first accommodating chamber, the second accommodating chamber and the third accommodating chamber are increased in sequence; the culture flask body is provided with a first communicating port, a second communicating port and a third communicating port, which are connected with the first accommodating chamber, the second accommodating chamber and the third accommodating chamber in sequence; the first communicating port, the second communicating port and the third communicating port are provided with a first sliding block, a second sliding block and a third sliding block that can slide in sequence; the culture flask body is provided with a blocking portion, a ventilating portion and a accommodating portion; wherein the blocking portion is used to isolate the first accommodating chamber, the second accommodating chamber and the third accommodating chamber from external air; the ventilating portion is used to connect the first accommodating chamber, the second accommodating chamber and the third accommodating chamber with external air; and the accommodating portion is used to place cells into the first accommodating chamber, the second accommodating chamber and the third accommodating chamber.

[0007] As a preferred embodiment, the culture bottle body is provided with a first sliding groove, a second sliding groove and a third sliding groove at the positions where the first connecting port, the second connecting port and the third connecting port are provided; the bottom sides of the first sliding groove, the second sliding groove and the third sliding groove are provided with limiting grooves; the sides of the first sliding block, the second sliding block and the third sliding block are provided with sliding limit blocks, and the sliding limit block of the first sliding block can be passed through and slid on the limiting groove of the first sliding groove; the sliding limit block of the second sliding block can be passed through and slid on the limiting groove of the second sliding groove; the sliding limit block of the third sliding block can be passed through and slid on the limiting groove of the third sliding groove.

[0008] As a preferred solution, the length of the first sliding groove is shorter than that of the second sliding groove, and the length of the second sliding groove is shorter than that of the third sliding groove.

[0009] As a preferred solution, the culture bottle body is provided with label grooves on the sides of the first sliding groove, the second sliding groove and the third sliding groove, and the label grooves are used to place cell labels.

[0010] As a preferred solution, the first sliding block is provided with a first anti-slip groove, the second sliding block is provided with a second anti-slip groove, and the third sliding block is provided with a third anti-slip groove; the number of the first anti-slip grooves is smaller than the number of the second anti-slip grooves, and the number of the second anti-slip grooves is smaller than the number of the third anti-slip grooves.

[0011] As a preferred solution, the bottoms of the first accommodating chamber, the second accommodating chamber and the third accommodating chamber are provided with arc-shaped surfaces, and the arc-shaped surfaces are used to gather the cell fluid.

[0012] As a preferred solution, test tubes are provided at the bottom of the first accommodating chamber, the second accommodating chamber and the third accommodating chamber, and the test tubes are passed through the side wall of the culture bottle body.

[0013] As a preferred embodiment, the side wall of the culture bottle body is provided with a through cylinder, the outer peripheral wall of the through cylinder is provided with an external thread, and the inner peripheral wall of the test tube is provided with an internal thread threadedly connected to the external thread; the through cylinder includes a through hole connected to the first accommodating chamber, the second accommodating chamber and the third accommodating chamber, and the test tube includes a test tube column, and the test tube column passes through the through hole and is connected to the first accommodating chamber, the second accommodating chamber and the third accommodating chamber.

[0014] Compared with the prior art, the present application has the following beneficial effects: fast switching. The fully enclosed separate cell culture flask of the present application generally includes a plurality of independent accommodating chambers, which are connected by corresponding connecting ports. A sliding block is provided in each accommodating chamber to meet the requirements of different culture stages. By adjusting the position of the sliding block, the isolation or connection between each accommodating chamber and the external air can be controlled, thereby meeting the oxygen demand at different stages of the cell culture process. This improves the problem that when the lid on the culture flask needs to be switched to meet the oxygen demand of the cells at different stages, the lid on the culture flask needs to be replaced, which has cumbersome replacement steps and cannot be quickly switched to meet the oxygen demand at different stages of the cell culture process. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0016] The structures, proportions, sizes, etc. depicted in the drawings of this specification are only used to match the contents disclosed in this specification so as to facilitate understanding and reading by those familiar with this technology. They are not intended to limit the conditions under which the present invention can be implemented, and therefore have no substantive technical significance. Any structural modifications, changes in proportional relationships, or adjustments in size, without affecting the efficacy and objectives that can be achieved by the present invention, should still fall within the scope of the technical contents disclosed in the present invention.

[0017] Figure 1 This is a schematic diagram of the overall structure of a fully enclosed separation cell culture flask in an embodiment of the present application;

[0018] Figure 2This is a schematic diagram of an explosion of a fully enclosed detachable cell culture bottle in an embodiment of the present application;

[0019] Figure 3 It is a semi-sectional schematic diagram of a fully enclosed separation cell culture flask in an embodiment of the present application.

[0020] Description of reference numerals:

[0021] 1. Culture bottle body; 11. First accommodating chamber; 111. First communication port; 12. Second accommodating chamber; 121. Second communication port; 13. Third accommodating chamber; 131. Third communication port; 14. First sliding groove; 15. Second sliding groove; 16. Third sliding groove; 17. Limiting groove; 18. Arc surface; 19. Through-center cylinder; 191. External thread; 192. Through-center hole; 10. Label slot; 2. First sliding block; 21. First anti-slip groove; 3. Second sliding block; 31. Second anti-slip groove; 4. Third sliding block; 41. Third anti-slip groove; 5. Blocking part; 6. Breathable part; 7. Accommodating part; 8. Sliding limiting block; 9. Test tube; 91. Internal thread; 92. Test tube column. DETAILED DESCRIPTION

[0022] In order to make the purpose, features, and advantages of the present invention more obvious and easy to understand, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the embodiments described below are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0023] In the description of the present invention, it should be understood that the terms "upper," "lower," "top," "bottom," "inner," "outer," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate the description of the present invention and simplify the description. They are not intended to indicate or imply that the devices or elements referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on the present invention. It should be noted that when a component is considered to be "connected" to another component, it may be directly connected to the other component or there may be a centrally located component.

[0024] The technical solution of the present invention will be further described below with reference to the accompanying drawings and through specific implementation methods.

[0025] Example 1:

[0026] like Figures 1 to 3As shown, a fully enclosed separated cell culture flask includes a culture flask body 1, which includes a first accommodating chamber 11, a second accommodating chamber 12 and a third accommodating chamber 13; wherein the accommodating spaces of the first accommodating chamber 11, the second accommodating chamber 12 and the third accommodating chamber 13 increase in sequence.

[0027] By designing the first accommodating chamber 11, the second accommodating chamber 12 and the third accommodating chamber 13 of the culture bottle to increase the accommodating spaces in sequence, the storage of different cell liquid amounts can be met, thereby increasing the adaptability and flexibility of the culture bottle.

[0028] The culture bottle body 1 is provided with a first communication port 111 , a second communication port 121 and a third communication port 131 which are connected to the first accommodating chamber 11 , the second accommodating chamber 12 and the third accommodating chamber 13 in sequence.

[0029] The first communication port 111 , the second communication port 121 and the third communication port 131 are connected in sequence to the first accommodating chamber 11 , the second accommodating chamber 12 and the third accommodating chamber 13 , so that the accommodating chambers can be filled with cell fluid for storage.

[0030] A first sliding block 2 , a second sliding block 3 , and a third sliding block 4 are slidably disposed on the first communication port 111 , the second communication port 121 , and the third communication port 131 .

[0031] By sequentially arranging a first sliding block 2, a second sliding block 3, and a third sliding block 4 that can slide on the first connecting port 111, the second connecting port 121, and the third connecting port 131, flexible control of each accommodating cavity is achieved, and the isolation or connection state of each accommodating cavity with the outside world can be adjusted as needed to meet the different needs of cells at different culture stages.

[0032] like Figures 1 to 3 As shown, finally, a blocking portion 5, a ventilating portion 6 and a receiving portion 7 are provided on the culture bottle body 1; wherein, a blocking portion 5, a ventilating portion 6 and a receiving portion 7 are all provided on the first sliding block 2, the second sliding block 3 and the third sliding block 4.

[0033] The first accommodating chamber 11 is isolated from the outside air by the blocking portion 5 on the first sliding block 2; the first accommodating chamber 11 is connected to the outside air by the air permeable portion 6 on the first sliding block 2; the cells are placed in the first accommodating chamber 11 through the accommodating portion 7 on the first sliding block 2; the second accommodating chamber 12 is isolated from the outside air by the blocking portion 5 on the second sliding block 3; the second accommodating chamber 12 is connected to the outside air by the air permeable portion 6 on the second sliding block 3; the cells are placed in the second accommodating chamber 12 through the accommodating portion 7 on the second sliding block 3; the third accommodating chamber 13 is isolated from the outside air by the blocking portion 5 on the third sliding block 4; the third accommodating chamber 13 is connected to the outside air by the air permeable portion 6 on the third sliding block 4; and the cells are placed in the third accommodating chamber 13 through the accommodating portion 7 on the third sliding block 4.

[0034] By respectively arranging the blocking portion 5, the breathable portion 6 and the accommodating portion 7 on the first sliding block 2, the second sliding block 3 and the third sliding block 4, the effective isolation, breathable and cell fluid insertion functions of each accommodating cavity are achieved, thereby ensuring the stability and controllability of the environment during the cell culture process and further improving the efficiency and quality of cell culture.

[0035] In this embodiment, a fully enclosed separate cell culture flask is designed, specifically including a first accommodating chamber 11, a second accommodating chamber 12, and a third accommodating chamber 13 on the culture flask body 1, and a first communicating port 111, a second communicating port 121, and a third communicating port 131 that are connected in sequence are provided, thereby achieving connectivity between the first accommodating chamber 11, the second accommodating chamber 12, and the third accommodating chamber 13 and the outside; in addition, a slidable first sliding block 2, a second sliding block 3, and a third sliding block 4 are provided on each communicating port. Through the functional design of the blocking portion 5, the air permeable portion 6, and the accommodating portion 7 on the first sliding block 2, the second sliding block 3, and the third sliding block 4, flexible control of each accommodating chamber and effective placement of cells are achieved, so that the oxygen supply and isolation status of each accommodating chamber can be adjusted at any time as needed during the cell culture process to meet the culture requirements of cells at different stages, avoid the tedious steps of replacing the lid in traditional culture flasks, and improve the convenience of operation and the success rate of culture. At the same time, the fully enclosed design in which the first accommodating chamber 11, the second accommodating chamber 12 and the third accommodating chamber 13 are separated from each other also reduces the risk of contamination and cross infection, provides a more stable and safe culture environment, and contributes to the healthy growth of cells and the smooth progress of research.

[0036] Example 2:

[0037] like Figures 1 to 3As shown, a first sliding groove 14, a second sliding groove 15 and a third sliding groove 16 are provided at the positions where the first communicating port 111, the second communicating port 121 and the third communicating port 131 are provided on the culture bottle body 1, and an inverted "T"-shaped limiting groove 17 is provided on the side of the bottom of the first sliding groove 14, the second sliding groove 15 and the third sliding groove 16; and a sliding limiting block 8 is provided on the side of the first sliding block 2, the second sliding block 3 and the third sliding block 4; wherein, the sliding limiting block 8 of the first sliding block 2 can be inserted and slid on the limiting groove 17 of the first sliding groove 14; and the sliding limiting block 8 of the second sliding block 3 can be inserted and slid on the limiting groove 17 of the second sliding groove 15; and the sliding limiting block 8 of the third sliding block 4 can be inserted and slid on the limiting groove 17 of the third sliding groove 16.

[0038] By arranging a first sliding groove 14, a second sliding groove 15 and a third sliding groove 16 on the culture bottle body 1, and arranging a limiting groove 17 on the side of the bottom of each sliding groove, stable sliding of the sliding block in the sliding groove is achieved, and the sliding block is prevented from being offset or falling off during the sliding process, thereby ensuring the effective isolation and connection of each accommodating chamber during the cell culture process.

[0039] The length of the first sliding groove 14 is smaller than that of the second sliding groove 15 , and the length of the second sliding groove 15 is smaller than that of the third sliding groove 16 .

[0040] By designing the length of the first slide groove 14 to be smaller than that of the second slide groove 15, and the length of the second slide groove 15 to be smaller than that of the third slide groove 16, it is possible to distinguish the first accommodating chamber 11, the second accommodating chamber 12 and the third accommodating chamber 13 by looking at the length of each slide groove, thereby preventing the problem of easy confusion when collecting different cell fluids.

[0041] like Figures 1 to 3 As shown, label grooves 10 are provided on the sides of the first sliding groove 14 , the second sliding groove 15 and the third sliding groove 16 of the culture bottle body 1 , and the label grooves 10 are used to place cell labels.

[0042] The convenient placement of cell labels enables researchers to clearly identify the cell types and culture stages cultured in each chamber, thereby improving the management efficiency of cell culture.

[0043] The first sliding block 2 is provided with a first anti-slip groove 21, the second sliding block 3 is provided with a second anti-slip groove 31, and the third sliding block 4 is provided with a third anti-slip groove 41; the number of the first anti-slip grooves 21 is smaller than the number of the second anti-slip grooves 31, and the number of the second anti-slip grooves 31 is smaller than the number of the third anti-slip grooves 41.

[0044] By providing different numbers of anti-slip grooves on the first sliding block 2, the second sliding block 3 and the third sliding block 4, the first accommodating chamber 11, the second accommodating chamber 12 and the third accommodating chamber 13 are further distinguished, and the anti-slip effect of the sliding blocks during operation is enhanced to prevent the sliding blocks from sliding out of control due to improper operation, thereby ensuring the safety and stability of the cell culture process.

[0045] like Figures 1 to 3 As shown, arc surfaces 18 are provided at the bottom of the first accommodating chamber 11 , the second accommodating chamber 12 and the third accommodating chamber 13 , and the arc surfaces 18 are used to gather the cell fluid.

[0046] By providing the arcuate surfaces 18 at the bottom of the first accommodating chamber 11 , the second accommodating chamber 12 and the third accommodating chamber 13 , effective convergence of the cell fluid is achieved, ensuring uniform distribution of cells during the culture process, thereby improving the effect and quality of cell culture.

[0047] A dip tube 9 is provided at the bottom of the first accommodating chamber 11 , the second accommodating chamber 12 and the third accommodating chamber 13 . The dip tube 9 is passed through the side wall of the culture bottle body 1 .

[0048] By arranging the dip tubes 9 at the bottom of the first accommodating chamber 11, the second accommodating chamber 12 and the third accommodating chamber 13, convenient collection of cell fluid is achieved, avoiding the cumbersome operation problems in traditional cell culture bottles, and improving the convenience and efficiency of operation.

[0049] like Figures 1 to 3 As shown, a through cylinder 19 is provided on the side wall of the culture bottle body 1, an external thread 191 is provided on the outer peripheral wall of the through cylinder 19, and an internal thread 91 is provided on the inner peripheral wall of the test tube 9, which is threadedly connected to the external thread 191; the through cylinder 19 includes a through hole 192 connected to the first accommodating chamber 11, the second accommodating chamber 12 and the third accommodating chamber 13, and the test tube 9 includes a test tube column 92, and the test tube column 92 passes through the through hole 192 and is connected to the first accommodating chamber 11, the second accommodating chamber 12 and the third accommodating chamber 13.

[0050] By arranging a through cylinder 19 and a dip tube 9 on the side wall of the culture bottle body 1, and arranging an external thread 191 on the outer peripheral wall of the through cylinder 19 and an internal thread 91 on the inner peripheral wall of the dip tube 9, a firm connection of the dip tube 9 is achieved, ensuring the sealing and stability of the cell fluid during the collection and addition process, preventing the cell fluid from leaking or being contaminated, and thus ensuring the safety and reliability of cell culture.

[0051] In this embodiment, by providing a first sliding groove 14, a second sliding groove 15, and a third sliding groove 16 on the culture bottle body 1, and providing a limiting groove 17 on the side of the bottom of each sliding groove, the stable sliding of the first sliding block 2, the second sliding block 3, and the third sliding block 4 in the sliding groove is ensured, preventing the sliding blocks from shifting or falling off during the sliding process. At the same time, by designing the length of the first sliding groove 14 to be smaller than the length of the second sliding groove 15, and the length of the second sliding groove 15 to be smaller than the length of the third sliding groove 16, segmented control of the sliding blocks in different sliding grooves is achieved, so that each sliding block can accurately control the connectivity state of the corresponding accommodating cavity, meeting the different needs of cells in different culture stages. In addition, a label groove 10 is provided on the side of the sliding groove for placing cell labels, which makes it convenient for researchers to clearly identify the cell types and culture stages cultured in each accommodating cavity, thereby improving the management efficiency of cell culture and the repeatability of experiments. Different numbers of anti-skid grooves are provided on the sliding block to enhance the anti-skid effect of the sliding block during operation, and prevent the sliding block from sliding out of control due to improper operation, thereby ensuring the safety and stability of the cell culture process. By providing an arc-shaped surface 18 at the bottom of the accommodating chamber, effective convergence of cell fluid is achieved, ensuring the uniform distribution of cells during the culture process, and improving the effect and quality of cell culture. By providing a dip tube 9 at the bottom of the accommodating chamber, convenient collection and addition of cell fluid are achieved, avoiding the cumbersome operation problems in traditional cell culture bottles, and improving the convenience and efficiency of operation. Finally, by providing a through cylinder 19 and a dip tube 9 on the side wall of the culture bottle body 1, and providing an external thread 191 on the outer peripheral wall of the through cylinder 19, and providing an internal thread 91 on the inner peripheral wall of the dip tube 9, a firm connection of the dip tube 9 is ensured, and the sealing and stability of the cell fluid during the collection and addition process are achieved, preventing the cell fluid from leaking or being contaminated, thereby ensuring the safety and reliability of cell culture.

[0052] As described above, the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A fully enclosed separation cell culture flask, characterized in that: The culture bottle body (1) comprises a first accommodating chamber (11), a second accommodating chamber (12) and a third accommodating chamber (13), wherein the accommodating spaces of the first accommodating chamber (11), the second accommodating chamber (12) and the third accommodating chamber (13) increase in sequence; The culture bottle body (1) is provided with a first communication port (111), a second communication port (121) and a third communication port (131) which are connected with the first accommodating cavity (11), the second accommodating cavity (12) and the third accommodating cavity (13) in sequence; A first sliding block (2), a second sliding block (3) and a third sliding block (4) that can slide are sequentially provided on the first communicating port (111), the second communicating port (121) and the third communicating port (131); The culture bottle body (1) is provided with a blocking portion (5), a ventilating portion (6) and a receiving portion (7); wherein the blocking portion (5) is used to isolate the first receiving chamber (11), the second receiving chamber (12) and the third receiving chamber (13) from the outside air; the ventilating portion (6) is used to connect the first receiving chamber (11), the second receiving chamber (12) and the third receiving chamber (13) with the outside air; and the receiving portion (7) is used to place cells into the first receiving chamber (11), the second receiving chamber (12) and the third receiving chamber (13).

2. The fully enclosed separation cell culture flask according to claim 1, characterized in that: The culture bottle body (1) is provided with a first sliding groove (14), a second sliding groove (15) and a third sliding groove (16) at the positions where the first communicating port (111), the second communicating port (121) and the third communicating port (131) are provided; Limiting grooves (17) are provided on the sides of the bottoms of the first sliding groove (14), the second sliding groove (15) and the third sliding groove (16); Sliding limit blocks (8) are provided on the sides of the first sliding block (2), the second sliding block (3) and the third sliding block (4); the sliding limit blocks (8) of the first sliding block (2) can be passed through and slid on the limiting groove (17) of the first sliding groove (14); The sliding limit block (8) of the second sliding block (3) can be inserted and slid on the limit groove (17) of the second sliding groove (15); The sliding limit block (8) of the third sliding block (4) can be inserted and slid on the limit groove (17) of the third sliding groove (16).

3. The fully enclosed separation cell culture flask according to claim 2, characterized in that: The length of the first sliding groove (14) is shorter than that of the second sliding groove (15), and the length of the second sliding groove (15) is shorter than that of the third sliding groove (16).

4. The fully enclosed separation cell culture flask according to claim 2, characterized in that: The culture bottle body (1) is provided with label grooves (10) on the sides of the first sliding groove (14), the second sliding groove (15) and the third sliding groove (16), and the label grooves (10) are used for placing cell labels.

5. The fully enclosed separation cell culture flask according to claim 1, characterized in that: The first sliding block (2) is provided with a first anti-slip groove (21), the second sliding block (3) is provided with a second anti-slip groove (31), and the third sliding block (4) is provided with a third anti-slip groove (41); The number of the first anti-slip grooves (21) is smaller than the number of the second anti-slip grooves (31), and the number of the second anti-slip grooves (31) is smaller than the number of the third anti-slip grooves (41).

6. The fully enclosed separation cell culture flask according to claim 1, characterized in that: The bottoms of the first accommodating chamber (11), the second accommodating chamber (12) and the third accommodating chamber (13) are provided with arcuate surfaces (18), and the arcuate surfaces (18) are used to gather cell fluid.

7. The fully enclosed separation cell culture flask according to claim 1, characterized in that: A dip tube (9) is provided at the bottom of the first accommodating chamber (11), the second accommodating chamber (12) and the third accommodating chamber (13), and the dip tube (9) is passed through the side wall of the culture bottle body (1).

8. The fully enclosed separation cell culture flask according to claim 7, characterized in that: The side wall of the culture bottle body (1) is provided with a through-hole cylinder (19), the outer peripheral wall of the through-hole cylinder (19) is provided with an external thread (191), and the inner peripheral wall of the dip tube (9) is provided with an internal thread (91) threadedly connected to the external thread (191); The through-hole cylinder (19) includes a through-hole (192) communicating with the first accommodating chamber (11), the second accommodating chamber (12) and the third accommodating chamber (13); the dip tube (9) includes a test tube column (92); the test tube column (92) passes through the through-hole (192) and communicates with the first accommodating chamber (11), the second accommodating chamber (12) and the third accommodating chamber (13).

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