Bottom transparent plate for tissue transparency detection

Abstract

The utility model relates to a bottom transparent plate for tissue transparency detection. The bottom transparent plate comprises a light-transmitting layer and a light-shielding layer arranged on the light-transmitting layer, the shading layer is provided with through holes which are arranged in rows and columns, and a transverse graduated scale and a longitudinal graduated scale which are perpendicular to each other; the connecting line of the central points of the through holes in each row is parallel to the transverse graduated scale, and the connecting line of the central points of the through holes in each column is parallel to the longitudinal graduated scale; the transverse graduated scale and the longitudinal graduated scale are both light-transmitting; the hole diameter of the through holes is smaller than 1 mm, and the distance between the through holes is not larger than 10 mm. The tissue transparency detection accuracy can be improved.

Description

Technical Field

[0001] The present application relates to the technical field of detection equipment, and more specifically, to a bottom transparent plate for tissue transparency detection. Background Art

[0002] Tissue transparency is a key step in 3D tissue imaging. Decolorization, decalcification, and fat removal are performed on fixed tissue using hydrophilic reagents or organic solvents. High-refractive-index media are then used to match the tissue's refractive index, reducing light scattering and achieving optical transparency, thereby increasing imaging depth and image contrast. Therefore, tissue transparency has a significant impact on subsequent 3D tissue imaging. Currently, tissue transparency is typically determined manually by observing tissue placed on a transparent substrate.

[0003] Existing bottom transparent plates are generally composed of vertical and horizontal grid lines. The degree of bending and deformation of the grid lines can be used to manually assess tissue transparency. To facilitate human visual recognition, the side length of the grid formed by the grid lines is not less than 1mm.

[0004] However, the bottom transparent plate is designed for manual observation, and the grid size is large, resulting in a large minimum unit that can be analyzed. It is unable to perform more detailed transparency detection of the tissue and is not suitable for software-based quantitative analysis. Utility Model Content

[0005] The present application is provided to solve the above-mentioned problems existing in the prior art. According to the bottom transparent plate for tissue transparency detection according to the embodiment of the present application, the accuracy of tissue transparency detection can be improved.

[0006] The embodiment of the present application provides a bottom transparent plate for tissue transparency detection, comprising: a light-transmitting layer, and a light-shielding layer disposed on the light-transmitting layer;

[0007] The light shielding layer has through holes arranged in rows and columns, and a horizontal scale and a vertical scale perpendicular to each other;

[0008] The line connecting the center points of the through holes in each row is parallel to the horizontal scale, and the line connecting the center points of the through holes in each column is parallel to the vertical scale;

[0009] The transverse scale and the longitudinal scale are both light-transmissive;

[0010] The aperture of the through holes is less than 1 mm, and the spacing between the through holes is no more than 10 mm.

[0011] In one embodiment of the present application, the spacing between the through holes is [20 μm-10 mm].

[0012] In one embodiment of the present application, the through hole has a pore diameter of [2 μm-1 mm].

[0013] In one embodiment of the present application, the shape of the through hole is any one of a circle, a triangle, a square, a rectangle, an ellipse and a five-pointed star.

[0014] In one embodiment of the present application, the light-transmitting layer is any one of quartz glass, optical glass and polymer transparent material.

[0015] In one embodiment of the present application, the light-shielding layer is a chrome-plated layer.

[0016] In one embodiment of the present application, the light-shielding layer is bonded to the light-transmitting layer through a coating.

[0017] In one embodiment of the present application, the light-shielding layer is formed by spraying a light-shielding material on the light-transmitting layer.

[0018] In one embodiment of the present application, the transverse scale and the longitudinal scale both include scale lines and digital markings, and the transverse scale and the longitudinal scale are hollow structures.

[0019] Using the transparent bottom plate according to various embodiments of the present application, the through-holes are smaller, and therefore the minimum unit of analysis is smaller, facilitating more precise quantitative analysis and improving the accuracy of transparency testing. The translucent horizontal and vertical scales on the transparent bottom plate allow for precise positioning of the through-holes, improving the accuracy of transparency testing. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In the drawings, which are not necessarily drawn to scale, the same reference numerals may describe similar components in different views. The same reference numerals with letter suffixes or different letter suffixes may represent different instances of similar components. The accompanying drawings generally illustrate various embodiments by way of example and not limitation, and together with the description and claims, serve to illustrate the disclosed embodiments. Where appropriate, the same reference numerals are used throughout the drawings to refer to the same or similar parts. Such embodiments are illustrative and are not intended to be exhaustive or exclusive of the embodiments of the present apparatus or method.

[0021] Figure 1 is a top view of a bottom transparent plate provided by one embodiment of the present application;

[0022] Figure 2 is a side view of a bottom transparent plate provided by one embodiment of the present application;

[0023] Figure 3 This is a partially enlarged schematic diagram of a bottom transparent plate provided by one embodiment of the present application;

[0024] Figure 4This is a partially enlarged schematic diagram of a bottom transparent plate provided in another embodiment of the present application. DETAILED DESCRIPTION

[0025] To enable those skilled in the art to better understand the technical solution of the present application, the present application is described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments of the present application are further described in detail below in conjunction with the accompanying drawings and specific embodiments, but are not intended to limit the present application. The terms "first," "second," and "third" used in this application are merely intended to distinguish corresponding features, and do not necessarily represent a need for such an order, nor do they necessarily represent only the singular form.

[0026] like Figure 1 and Figure 2 As shown, an embodiment of the present application provides a bottom transparent plate for tissue transparency detection, comprising: a light-transmitting layer 15 and a light-shielding layer 14 disposed on the light-transmitting layer.

[0027] The light shielding layer 14 has through holes arranged in rows and columns, that is, Figure 1 The light-transmitting micropores 13 shown in FIG. 1 further include a transverse scale 11 and a longitudinal scale 12 perpendicular to each other on the light-shielding layer.

[0028] The line connecting the center points of the through holes 13 in each row is parallel to the horizontal scale 11 , and the line connecting the center points of the through holes 13 in each column is parallel to the vertical scale 12 .

[0029] Both the horizontal scale 11 and the vertical scale 12 are light-transmissive.

[0030] The diameter of the through holes 13 is less than 1 mm, and the spacing between the through holes 13 is no more than 10 mm.

[0031] In the embodiments of this application, the smaller size of the through-holes allows for a smaller minimum unit of analysis, facilitating more precise quantitative analysis and improving the accuracy of transparency testing. The translucent horizontal and vertical scales on the bottom transparent plate allow for precise positioning of the through-holes, improving the accuracy of transparency testing.

[0032] In one embodiment of the present application, the bottom transparent plate can be divided into different detection areas, each with different detection accuracy. For example, from Area 1 to Area 3, the diameter and spacing of the through holes gradually increase, resulting in the highest detection accuracy in Area 1. Within the same detection area, the through holes have the same size, the same lateral spacing between the through holes, and the same longitudinal spacing between the through holes. The lateral spacing and longitudinal spacing can be the same or different.

[0033] It should be noted that, in actual application scenarios, the vertical scale and the horizontal scale may include only scale lines, or may include both scale lines and digital markings.

[0034] In one embodiment of the present application, to make the scale display more obvious, the horizontal and vertical scales are hollowed out. The edges of the scale lines and numerical markings can be hollowed out, or the scale lines and numerical markings can be hollowed out. Of course, the scale lines and numerical markings can also be formed of a transparent material embedded in the light-shielding layer, and the implementation is not limited to one method.

[0035] In one embodiment of the present application, the spacing between the through holes is [20 μm-10 mm]. For example, the spacing between the through holes is 20 μm, or 5 mm, or 10 mm. Through this embodiment of the present application, the through hole spacing can reach the micron level, thereby achieving more accurate detection. If the lateral spacing is different from the longitudinal spacing, the lateral spacing and the longitudinal spacing are both within the range of [20 μm-10 mm].

[0036] In one embodiment of the present application, the aperture of the through hole is [2 μm-1 mm), such as 2 μm, 50 μm, or 0.99 mm. If the through hole is circular, its diameter is [2 μm-1 mm]. If the through hole is elliptical, its major axis is [2 μm-1 mm]. If the through hole is square, its side length is [2 μm-1 mm]. By reducing the size of the through hole, the embodiment of the present application reduces the minimum detectable area unit, thereby improving detection accuracy.

[0037] In one embodiment of the present application, the through-holes may be in regular shapes such as circles, triangles, squares, rectangles, ellipses, and pentagrams, to facilitate subsequent quantitative calculations and improve efficiency. For example, a circle, due to its regular shape, is easier to characterize morphological features such as roundness. Therefore, using a circle can improve efficiency in subsequent transparency calculations.

[0038] In one embodiment of the present application, the light-transmitting layer is any one of quartz glass, optical glass, and a transparent polymer material. The transparent polymer material may be polystyrene, acrylonitrile-styrene copolymer, acrylonitrile-butadiene-styrene copolymer, polycarbonate, or the like. Quartz glass, for example, is a light-transmitting layer that exhibits high transparency, stability, and strength.

[0039] In one embodiment of the present application, the light-shielding layer is a chrome-plated layer. The chrome-plated light-shielding layer can effectively reduce light penetration and provide a good light-shielding effect. Chromium has good chemical stability, heat resistance, and wear resistance, and can maintain flatness and light-shielding effect for a long time.

[0040] In one embodiment of the present application, in addition to electroplating, the light-shielding layer can also be attached to the light-transmitting layer by lamination or spraying. For example, the lamination material can be a polyethylene film that has undergone a surface treatment such as corona treatment, or a polypropylene film. The sprayed light-shielding material can be polystyrene particles, nanomaterials, etc.

[0041] Through holes can be prepared by etching, laser drilling, and other processes. The bottom transparent plate processed in this way has high precision of through holes and hole spacing, which can achieve high-precision transparency testing. The through holes are not easily blocked by tissue samples and are easy to clean.

[0042] In actual application scenarios, the transparency of tissue can be judged by analyzing the changes in parameters such as brightness, center of mass displacement, number, and morphology (such as roundness, aspect ratio, and symmetry) of each through hole in the bottom transparent plate, thereby achieving the purpose of accurately quantifying tissue transparency.

[0043] In one embodiment of the present application, reference Figure 3 , through hole on the bottom plate, i.e. Figure 3 The light-transmitting holes (black dots) shown in FIG are circular holes with an aperture of 100 μm. The lateral spacing between the through holes is 0.10 mm, and the longitudinal spacing is 1 mm.

[0044] In one embodiment of the present application, Figure 4 As shown, the aperture of the through hole is 20 μm, and the spacing between the through holes is 0.10 mm, that is, the horizontal spacing and the vertical spacing are 0.10 mm.

[0045] Furthermore, although exemplary embodiments have been described herein, the scope includes any and all embodiments based on the present application having equivalent elements, modifications, omissions, combinations (eg, schemes that intersect various embodiments), adaptations, or changes.

[0046] The elements of the claims are to be interpreted broadly based on the language employed in the claims and not limited to the examples described in this specification or during the prosecution of the application, which examples are to be construed as non-exclusive. Accordingly, it is intended that the specification and examples be considered as examples only, with the true scope and spirit being indicated by the claims and their full scope of equivalents.

[0047] The above description is intended to be illustrative rather than restrictive. For example, the above examples (or one or more of their solutions) may be used in combination with each other. For example, a person of ordinary skill in the art may use other embodiments when reading the above description. In addition, in the above-mentioned specific embodiments, various features may be grouped together to simplify the application. This should not be interpreted as an intention that a disclosed feature that is not required to be protected is necessary for any claim. On the contrary, the subject matter of the present utility model may be less than all the features of a specific disclosed embodiment. Thus, the claims are incorporated into the specific embodiments as examples or embodiments, wherein each claim is independently a separate embodiment, and it is considered that these embodiments can be combined with each other in various combinations or arrangements. The scope of the present utility model should be determined with reference to the appended claims and the full scope of equivalents to which these claims are entitled.

Claims

1. A bottom transparent plate for tissue transparency detection, characterized in that: include: a light-transmitting layer, and a light-shielding layer disposed on the light-transmitting layer; The light shielding layer has through holes arranged in rows and columns, and a horizontal scale and a vertical scale perpendicular to each other; The line connecting the center points of the through holes in each row is parallel to the horizontal scale, and the line connecting the center points of the through holes in each column is parallel to the vertical scale; The transverse scale and the longitudinal scale are both light-transmissive; The aperture of the through holes is less than 1 mm, and the spacing between the through holes is no more than 10 mm.

2. The bottom transparent plate according to claim 1, wherein: The distance between the through holes is [20 μm-10 mm].

3. The bottom transparent plate according to claim 1, wherein: The through-holes have a pore diameter of [2 μm-1 mm].

4. The bottom transparent plate according to claim 1, wherein: The through hole has a shape of any one of a circle, a triangle, a rectangle, an ellipse and a five-pointed star.

5. The bottom transparent plate according to claim 1, wherein: The light-transmitting layer is any one of quartz glass, optical glass and polymer transparent material.

6. The bottom transparent plate according to claim 1, wherein: The light-shielding layer is a chrome-plated layer.

7. The bottom transparent plate according to claim 1, wherein: The light-shielding layer is bonded to the light-transmitting layer through a laminating film.

8. The bottom transparent plate according to claim 1, wherein: The light-shielding layer is formed by spraying a light-shielding material on the light-transmitting layer.

9. The bottom transparent plate according to claim 1, wherein: The transverse scale and the longitudinal scale both include scale lines and digital markings, and the transverse scale and the longitudinal scale are hollow structures.

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