Multi-light-path concentration detection device

Abstract

The utility model provides a multi-light-path concentration detection device. Relates to the field of microbiological detection, and aims to solve the problem that the existing microbiological concentration detection equipment is difficult to accurately detect a suspension with relatively high concentration, a plurality of detection elements are arranged on a detection position and can respectively emit detection light beams, and the wavelengths of the detection light beams can be the same or can comprise at least two different wavelengths; the problem that traditional single-optical-path single-wavelength detection is difficult to deal with high-concentration detection can be solved, the detection position can be matched with cuvettes with multiple detection optical paths, the process of diluting suspension is omitted, the detection range is enlarged, and the detection precision is improved.

Description

Technical Field

[0001] The utility model relates to the field of microbial detection, in particular to a multi-light path concentration detection device. Background Art

[0002] Microbial culture is the foundation of bacterial identification and drug susceptibility testing. Based on clinical microbiological classification, microbial culture is divided into two categories: aerobic and anaerobic. Microbial concentration testing provides quantitative data, enabling researchers to accurately understand microbial growth and reproductive capacity. This is crucial for assessing microbial activity, physiological status, and metabolic capacity.

[0003] When testing the concentration of microorganisms, add the processed microbial suspension to a cuvette, and place the cuvette in a microbial concentration rapid tester for measurement to obtain the concentration. Conventional microbial concentration rapid testers use a single wavelength detection beam. When the concentration of the microbial suspension is high, it may exceed the linear detection range of the instrument, resulting in inaccurate measurement results. The microbial suspension needs to be diluted during processing to reduce the concentration of the microbial suspension so that it is within the appropriate measurement range of the measuring instrument or method. The dilution operation also has certain risks. The dilution operation requires precise control of the dilution multiple and operating steps, which can easily introduce contamination. At the same time, there may be errors in the dilution process, such as the inaccuracy of the pipette, the impurity of the diluent, manual operation errors, etc. These will introduce new error sources, resulting in the rapid tester's measurement accuracy being difficult to meet the requirements when dealing with microbial suspensions with higher concentrations. Utility Model Content

[0004] The purpose of the utility model is to address the defects of the existing technology and provide a multi-light path concentration detection device. A plurality of detection elements are arranged on the detection position, which can respectively emit detection light beams, and the wavelengths of the detection light beams can be the same or include at least two different wavelengths. This can solve the problem that the traditional single light path and single wavelength are difficult to cope with high concentration detection. The detection position can also match cuvettes with multiple detection light paths, eliminating the process of diluting the suspension, increasing the detection range, and improving the detection accuracy.

[0005] The first purpose of this utility model is to provide a multi-light path concentration detection device, which adopts the following technical solutions:

[0006] The invention comprises a base body, on which is provided at least one detection position for accommodating a detection container, and a plurality of detection elements mounted on the base body, each detection element comprising a receiver and a transmitter distributed on different sides of the detection position, the detection container being located between the receiver and the transmitter, the wavelengths of the light beams output by the transmitters of at least two different detection elements being different, and the different light beam wavelengths passing through different optical path positions of the detection container, and the receiver being used to receive the light beam after passing through the detection container.

[0007] Furthermore, the base is provided with a first detection position and a second detection position, the cross section of the first detection position is rectangular, and the cross section of the second detection position is circular, and the different detection positions are used to adapt to different detection containers.

[0008] Furthermore, the second detection position is a hole structure with an inclined axis, and a drainage hole connecting the inside of the hole structure and the outside of the base is provided at the bottom of the second detection position.

[0009] Furthermore, the drainage hole is inscribed in the hole structure corresponding to the second detection position, and the drainage hole is connected to the bottom end of the second detection position.

[0010] Furthermore, a plurality of detection elements are distributed correspondingly on the side of the first detection position, and a plurality of detection elements are distributed correspondingly on the circumference of the second detection position.

[0011] Furthermore, a detection hole is opened on the base, and the transmitter and receiver are respectively installed in the corresponding detection holes, and the wavelengths of the light beams output by all transmitters are different.

[0012] Furthermore, at least one of the detection positions of the substrate is matched with a cuvette, and the cuvette has different thicknesses corresponding to the transmission positions of different emitters to form different optical paths.

[0013] Furthermore, a matching tubular container is provided in the detection position of the base body, and the tubular container is coaxially distributed with the matching detection position.

[0014] Furthermore, the emitter includes a light-emitting element and a driver, and the output end of the light-emitting element faces the corresponding detection position to output a light beam that is transmitted to the detection container in the detection position.

[0015] Furthermore, a mounting groove is provided on the side wall of the base body for accommodating the receiver and the transmitter.

[0016] The second object of the present invention is to provide another optical path concentration detection device, which adopts the following technical solutions:

[0017] The invention comprises a base body, on which is provided at least one detection position for accommodating a detection container, and a plurality of detection elements mounted on the base body, each detection element comprising a receiver and a transmitter distributed on different sides of the detection position, the detection container being located between the receiver and the transmitter, at least two different transmitters outputting light beams of the same wavelength passing through the same optical path position of the detection container, and the receiver being used to receive the light beams after passing through the detection container.

[0018] Compared with the prior art, the advantages and positive effects of this utility model are:

[0019] (1) In order to solve the problem that the current microbial concentration detection equipment is difficult to accurately detect suspensions with higher concentrations, multiple detection elements are set at the detection position, which can emit detection light beams respectively, and the detection light beams include at least two detection light beams with different wavelengths, so as to solve the problem that the traditional single wavelength is difficult to cope with high concentration detection. The detection position can also match cuvettes with multiple detection optical path lengths, eliminating the process of diluting the suspension, increasing the detection range, and improving the detection accuracy.

[0020] (2) Multiple light beams passing through the same optical path position of the detection container can also use the same wavelength. Multiple emitters emit multiple wavelengths correspondingly. The detection container is subjected to transmission detection using light beams of the same wavelength to obtain multiple sets of data, thereby reducing the detection error caused by differences in microbial concentration distribution within the same detection area.

[0021] (3) For microbial suspensions with higher concentrations, it is difficult to meet the transmission requirements using a single-wavelength light beam, and the light beam transmission capability is poor. In this application, multiple optical paths and multiple wavelengths of detection light beams are used to pass through microbial suspensions with higher concentrations to meet the detection requirements. At the same time, the detection light beam can maintain accurate detection of low-concentration microbial suspensions. The detection results of multiple detection units assist each other and verify each other, thereby jointly improving the detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention.

[0023] Figure 1 This is a schematic diagram of the external structure of the multi-light path concentration detection device in an embodiment of the present utility model.

[0024] Figure 2 Schematic diagram of the internal structure of the second detection position in an embodiment of the present utility model.

[0025] Figure 3 Schematic diagram of the internal structure of the first detection position in an embodiment of the present utility model.

[0026] In the figure, 1. base, 2. mounting groove, 3. first detection position, 4. second detection position, 5. detection hole, 6. drainage hole. DETAILED DESCRIPTION

[0027] In a typical embodiment of the present invention, Figure 1-Figure 3 As shown, a multi-optical path concentration detection device is proposed.

[0028] During the cultivation of microbial suspensions, when the concentration of the microbial suspension is detected, when the OD value is less than 1.0, a traditional detector can be used to detect it through a detection beam with a wavelength of 600nm. When the OD value is far greater than 1.0, traditional detection equipment and detection methods will cause large deviations in the detection results. New error sources will be introduced by dilution, making it difficult to meet the requirements of detection accuracy. Based on this, the present embodiment provides a multi-light path concentration detection device. On the one hand, a plurality of detection elements acting on the detection position are configured with detection beams of multiple optical paths. A long optical path detection beam is used to detect low-concentration suspensions, and a short optical path detection beam is used to detect high-concentration suspensions. Different wavelengths can be selected for detection as needed to expand the measurement range, solving the problem that a single optical path and a single wavelength are difficult to cope with high-concentration detection. On the other hand, a cuvette with multiple detection optical paths is configured for the detection position. Different detection elements transmit different positions of the cuvette, thereby forming different detection optical paths, which has the effect of increasing the detection range.

[0029] like Figure 1 As shown, the multi-light path concentration detection device includes a substrate 1 and a detection element installed on the substrate 1. The detection element includes a transmitter and a receiver. The transmitter can output a detection light beam to project onto the detection container. Among the incident detection light beam, part of the detection light beam is absorbed by the suspension in the detection container, and part of the detection light beam is detected by the receiver after passing through the detection container. Based on the Lambert-Beer law, the OD value is calculated according to the measured transmitted light intensity and incident light intensity, thereby reflecting the concentration of suspended microorganisms.

[0030] Base 1 serves as the support and mounting platform for the entire detection device, ensuring the stability and accuracy of all components. It is designed with at least one detection position for accommodating a test container, such as a test tube, McFadden standard tube, or cuvette. The number of detection positions can be adjusted as needed, and their shape can be adapted to suit different testing requirements. The detection position is used to position the test container, ensuring that the detection beam accurately passes through the sample in the container.

[0031] In this embodiment, to meet the requirements of multi-spectral, multi-pathlength concentration detection, at least one detection position is configured to accommodate a special cuvette, referred to as first detection position 3. First detection position 3 can also accommodate cuvettes with a variety of detection pathlengths. The cuvettes have varying thicknesses corresponding to different detection beam positions, allowing the detection beam to pass through positions on the cuvette with varying pathlengths. This allows detection of different pathlengths without changing equipment, thereby expanding the detection range.

[0032] Each detection element consists of an emitter and a receiver, located on opposite sides of the detection container. The emitter transmits a detection beam as incident light into the detection container, while the receiver receives the transmitted light after passing through the detection container. The receiver receives the transmitted light and converts it into an electrical signal or other measurable signal, obtaining the intensity of the transmitted light for subsequent processing and analysis.

[0033] Depending on the measurement requirements, the emitters of two different detection elements can output beams of different wavelengths. These beams can be applied to the sample simultaneously or separately, enabling multi-wavelength detection. Multi-wavelength detection can provide richer detection information and improve detection accuracy and reliability.

[0034] By matching the different thicknesses at different positions on the cuvette, different light beam wavelengths will form different optical path lengths when passing through different thickness positions of the detection container, enabling the device to directly detect high-concentration samples without dilution, thereby increasing the detection range, simplifying the operating process and improving detection efficiency.

[0035] like Figure 2 As shown, two detection elements can be configured for the detection position, corresponding to two wavelengths of detection light beams. The wavelengths of the light beams can be configured as needed. In this embodiment, different wavelengths are used, and the two wavelengths are set to 600nm and 850nm respectively; Figure 3 As shown, three detection elements can be configured for the detection position, corresponding to three wavelengths of detection light beams, namely 600nm, 850nm and 1050nm. In other optional embodiments, other wavelengths can be used according to specific application scenarios. In this embodiment, the use of multiple light paths to simultaneously detect the same analyte can achieve high concentration detection that cannot be completed with a single light path and a single wavelength, and can achieve the detection of suspensions with OD values of 10, 25, 30, etc.

[0036] Traditional single-pathway, single-wavelength detection methods often have limitations when dealing with high-concentration samples, as high-concentration samples may cause the light signal to be too strong or too weak, thus affecting the accuracy of the test results. In this embodiment, this problem is effectively solved by introducing multi-path detection. Traditional detection methods usually require dilution of samples to adapt to the detection range, which not only increases the complexity of the operation but also may introduce errors. In this embodiment, by matching cuvettes with multiple detection light paths, the detection range is expanded, and high-concentration samples can be directly detected without dilution.

[0037] like Figure 1As shown, during the microbial cultivation process, different detection containers are used to perform detections of different needs. In order to meet this demand, in this embodiment, a first detection position 3 adapted to a special cuvette is provided on the base 1, and a second detection position 4 adapted to a tubular detection container is also provided. By designing detection positions with different cross sections, the device can adapt to detection containers of various shapes. The cross section of the first detection position 3 is rectangular, and the cross section of the second detection position 4 is circular. Different detection positions are used to adapt to different detection containers. The first detection position 3 can be adapted to a cuvette, and the second detection position 4 can be adapted to a McFadden standard tube, a test tube, etc. This increases the flexibility and versatility of use, and a suitable detection container can be selected according to specific experimental needs.

[0038] The second testing position 4 is designed as a hole structure with an inclined axis and equipped with a drainage hole 6. The tilted position makes it easier to pick up and place the test container. If the test container is broken or damaged, the provision of drainage hole 6 ensures that waste liquid or impurities can be easily removed during the experiment, avoiding damage to the device caused by the inability to drain liquid, thereby improving the cleanliness and efficiency of the experiment.

[0039] Specifically, such as Figure 2 As shown, in fields such as chemical analysis, biological experiments, and medical testing, containers of various shapes and lengths are often required to hold samples. For longer tubular containers, the traditional vertical placement of the test station is inconvenient during placement and removal. This is especially true when the test container contains liquid or is fragile. Careless handling can cause liquid spillage or damage to the container.

[0040] By setting the second detection position 4 as an inclined hole structure, it conforms to ergonomics. The inclined hole structure design fully considers the natural movement of the human arm when pulling out items. During the extraction process, the arm usually exerts an upward pulling force accompanied by a slight horizontal movement. The inclined hole structure allows the tubular container to move horizontally along the inclined angle while being pulled upward, thereby more smoothly detaching from the detection position and reducing the risk of jamming or breakage due to improper operation. Compared with vertical or horizontal hole structures, the inclined hole structure can provide a smoother transition during the extraction process, reducing wear and damage to the outer wall of the container.

[0041] In addition, the drainage hole 6 provided at the bottom can promptly discharge the liquid accumulated at the bottom of the detection position due to container damage, liquid splashing or other reasons, which helps to keep the detection position clean and dry and prevent the liquid from interfering with or damaging subsequent experiments; the design of the drainage hole 6 also avoids the problems of corrosion, mildew, etc. that may be caused by the long-term accumulation of liquid at the bottom of the detection position, thereby ensuring the long-term stable operation of the detection equipment.

[0042] The drainage hole 6 is inscribed in the hole structure of the second detection position 4 and connected to its bottom, ensuring that the drainage hole 6 can maximize the use of space while not affecting the transmission of the detection light beam and draining all the accumulated water at the bottom.

[0043] It should be pointed out that, in addition to the first detection position 3 and the second detection position 4, detection positions of other shapes can also be configured on the substrate 1. Similarly, in addition to configuring detection elements of two or three wavelengths for the detection position, detection elements of four, five or more wavelengths can also be added. The detection position can match different detection containers. For the same detection position, its specific size can also be designed according to demand. It is also possible to use auxiliary means to make the same detection position adapt to similar detection containers with different size parameters. For example, for the second detection position 4, when adapting to the tubular detection container, test tubes of various specifications can be placed, such as test tubes with a diameter of 10mm / 12mm / 15mm / 18mm / 20mm, and the corresponding detection optical path is different. After detection, its parameters are processed to meet the needs. In order to improve the detection accuracy, the hole structure corresponding to the test tube and the second detection position 4 can be kept coaxial by adding structures such as pads and retainers to keep its detection position stable.

[0044] like Figure 1 、 Figure 2 and Figure 3 As shown, multiple detection elements are distributed on the sides of the first detection position 3 and the circumference of the second detection position 4, allowing the device to perform multi-light path detection simultaneously or separately, improving detection efficiency and accuracy. At the same time, users can also choose to use some or all of the detection elements according to experimental requirements.

[0045] By opening the detection holes 5 on the base 1 and installing the transmitter and receiver in the corresponding detection holes 5, the stability and accuracy of the transmitter and receiver are ensured. At the same time, it is also convenient to maintain and replace these components.

[0046] All emitters output beams of different wavelengths, enabling multi-spectral detection. The emitters corresponding to different detection elements can simultaneously or separately emit beams of different wavelengths as incident light, providing more spectral information for container detection.

[0047] The cuvette has different thicknesses at the transmission positions corresponding to different emitters. These different thicknesses allow light beams of different wavelengths to pass through different beam positions on the cuvette. Differences in optical path lengths facilitate more accurate measurements of high-concentration suspensions. These differences in optical path length directly affect the attenuation of the light signal after passing through the suspension, thereby reflecting different information about the concentration of microorganisms in the suspension. By using multiple detection positions with different optical path lengths, even high-concentration liquids can meet transmission requirements through shorter optical path lengths, thereby improving detection accuracy and reliability.

[0048] In addition to the cuvette, the base 1 also features a detection station that matches the tubular container. The tubular container and its matching detection station are coaxially arranged to improve detection accuracy. This allows the McFadden standard tube used in the turbidimeter to be directly used in the second detection station 4 of the multi-optical concentration detection device.

[0049] The transmitter includes a light-emitting element and a driver. The output end of the light-emitting element is directed toward the corresponding detection position, so that the output light beam is transmitted to the detection container in the detection position. The light-emitting element has the characteristics of high light intensity, good monochromaticity, and strong coherence, and is suitable for spectral detection. The driver is responsible for controlling the switching and output power of the light-emitting element. The light-emitting element can be a visible light emitting element, an infrared light emitting element, etc., whichever can meet the detection requirements. The receiver receives the light beam after passing through the detection container and converts it into an electrical signal or other measurable signal. Common receiver types include photodiodes, photomultiplier tubes, charge-coupled devices (CCDs), CMOS image sensors, etc. Each receiver has its own specific sensitivity, spectral response range, noise level, dynamic range, etc., so it needs to be selected according to the specific application scenario.

[0050] The sidewalls of the base 1 are provided with mounting slots 2 for accommodating the receiver and transmitter. This not only saves space but also makes the installation of the receiver and transmitter more stable and convenient. It also allows users to adjust and replace them as needed.

[0051] The multi-path concentration detection device in this embodiment, through its ingenious design and innovative structure, enables direct detection of high-concentration samples, obtaining accurate measurement results without dilution. Its multi-path and multi-wavelength detection capabilities enhance detection accuracy and reliability, while the flexible detection positions and detection element layout increase flexibility and versatility.

[0052] Example 2

[0053] In another embodiment of the present invention, Figure 1-Figure 3 As shown, a multi-light path concentration detection device is provided, which differs from Example 1 in the configuration of the light beam output by the detection element.

[0054] In this embodiment, Figure 1-Figure 3 As shown, the structure of the substrate 1 is substantially the same as that in Example 1. Figure 2 As shown, two detection elements can be configured for the detection position, corresponding to two detection beams of the same wavelength. The wavelength of the light beam can be configured as needed. In this embodiment, the same wavelength is used, and the two wavelengths are set to 600nm or 850nm respectively. The detection beam of the same wavelength is used to transmit the detection container position with the same optical path; Figure 3As shown, three detection elements can be configured for the detection position, corresponding to the detection light beams of the same wavelength, and the wavelengths of the detection light beams output by the three detection elements are all set to 600nm, or all set to 850nm, or all set to 1050nm. In other optional embodiments, other wavelengths can be used according to specific application scenarios. In this embodiment, the use of multiple light paths with the same wavelength to simultaneously detect the same analyte can achieve high-concentration detection that cannot be completed under a single light path and a single wavelength, and can achieve detection of suspensions with OD values of 10, 25, 30, etc.

[0055] The wavelength of the light beams output by all emitters can be the same, or different wavelengths can be set as needed to achieve multi-path and multi-wavelength detection. The emitters corresponding to different detection elements can simultaneously or separately emit light beams of the required wavelength as incident light, providing more detection information for container inspection.

[0056] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A multi-optical concentration detection device, characterized in that: The invention comprises a base body, on which is provided at least one detection position for accommodating a detection container, and a plurality of detection elements mounted on the base body, each detection element comprising a receiver and a transmitter distributed on different sides of the detection position, the detection container being located between the receiver and the transmitter, the wavelengths of the light beams output by the transmitters of at least two different detection elements being different, and the different light beam wavelengths passing through different optical path positions of the detection container, and the receiver being used to receive the light beam after passing through the detection container.

2. The multi-path concentration detection device according to claim 1, wherein: The base is provided with a first detection position and a second detection position. The cross section of the first detection position is rectangular, and the cross section of the second detection position is circular. Different detection positions are used to adapt to different detection containers.

3. The multi-path concentration detection device according to claim 2, wherein: The second detection position is a hole structure with an inclined axis, and a drainage hole connecting the inside of the hole structure and the outside of the base is provided at the bottom of the second detection position.

4. The multi-light path concentration detection device according to claim 3, wherein: The drainage hole is inscribed with the hole structure corresponding to the second detection position, and the drainage hole is connected to the bottom end of the second detection position.

5. The multi-light path concentration detection device according to claim 2, 3 or 4, characterized in that: A plurality of detection elements are distributed correspondingly on the side of the first detection position, and a plurality of detection elements are distributed correspondingly on the circumference of the second detection position.

6. The multi-light path concentration detection device according to claim 1, 2, 3 or 4, characterized in that: The base is provided with detection holes, and the emitters and receivers are respectively installed in the corresponding detection holes. The wavelengths of the light beams output by all the emitters are different.

7. The multi-path concentration detection device according to claim 1, wherein: At least one of the detection positions of the substrate is matched with a cuvette, and the cuvette has different thicknesses corresponding to the transmission positions of different emitters to form different optical path lengths; the detection position of the substrate is also provided with a matched tubular container, and the tubular container is coaxially distributed with the detection position it matches.

8. The multi-optical path concentration detection device according to claim 1, wherein: The emitter includes a light emitting element and a driver. The output end of the light emitting element faces the corresponding detection position to output a light beam that is transmitted to the detection container in the detection position.

9. The multi-light path concentration detection device according to claim 1 or 8, characterized in that: The side wall of the base is provided with a mounting groove for accommodating the receiver and the transmitter.

10. A multi-light path concentration detection device, characterized in that: The invention comprises a base body, on which is provided at least one detection position for accommodating a detection container, and a plurality of detection elements mounted on the base body, each detection element comprising a receiver and a transmitter distributed on different sides of the detection position, the detection container being located between the receiver and the transmitter, at least two different transmitters outputting light beams of the same wavelength passing through the same optical path position of the detection container, and the receiver being used to receive the light beams after passing through the detection container.

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