Honeycomb nanofiber-based flexible probe medical dressing

Abstract

The utility model provides a honeycomb nanofiber-based flexible probe medical dressing, the shape of the medical dressing is a honeycomb formed by closely arranging hexagonal structure units, six edges of each hexagonal structure unit are detection parts, the middle surrounded part is a treatment part, and the detection parts are closely attached to the treatment part; the detection part is used for detecting the high active oxygen concentration of the wound and judging whether the wound is infected or not; the treatment part is used for treatment and infection resistance of wounds after skin tumor excision. The medical dressing is used for wound treatment and infection resistance after skin tumor excision, and meanwhile, the high active oxygen concentration of a wound is detected, and whether the wound is infected or not is judged.

Description

Technical Field

[0001] The utility model belongs to the technical field of new medical materials, and in particular relates to a honeycomb nanofiber-based flexible probe medical dressing. Background Art

[0002] With the development of nanotechnology, nanoprobes have been designed with an increasing number of functions, especially those combined with the diagnosis and treatment of serious diseases such as tumors. Therefore, we propose a new probe medical dressing that combines in vitro nanoprobe detection with multi-functional treatments through a honeycomb shape. Summary of the Invention

[0003] The utility model provides a honeycomb nanofiber-based flexible probe medical dressing, which is used for treating and resisting wounds after skin tumor resection and simultaneously detects high active oxygen concentration in the wound to determine whether the wound is infected.

[0004] The above-mentioned purpose of the utility model is achieved through the following technical solutions:

[0005] A honeycomb nanofiber-based flexible probe medical dressing, which is shaped like a honeycomb formed by closely arranged hexagonal structural units. The six sides of the hexagonal structural unit are the detection part, and the middle part surrounded by the detection part is the treatment part. The detection part and the treatment part are tightly fitted.

[0006] The detection part is used to detect the concentration of high reactive oxygen species (hROS) in the wound to determine whether the wound is infected;

[0007] The therapeutic part is used for the treatment of wounds after skin tumor excision and for anti-infection;

[0008] The detection part is a fluorescent probe based on gold nanoclusters stabilized by cellulose nanocrystals (CNCs), which is used to detect the concentration of highly reactive oxygen species (hROS). In the presence of highly reactive oxygen species (hROS), the blue fluorescence of the fluorescent probe is quenched.

[0009] The therapeutic component is a near-infrared (NIR) and pH-responsive cellulose nanofiber nanocage wound dressing (CNF NWD@ICG&DOX), designed to kill residual tumor cells, resist invasion by external bacterial pathogens, and eliminate biofilms in wounds following skin tumor resection. The NIR and pH-responsiveness act as an on / off switch, enabling the controlled and effective release of the drugs doxorubicin (DOX) and indocyanine green (ICG) in the dressing into the wound area, thereby killing residual tumor cells, resisting invasion by external bacterial pathogens, and eliminating biofilms. The ICG in the dressing detaches from and enters bacterial cells, releasing reactive oxygen species (ROS) upon exposure to near-infrared laser light. ROS attack the bacterial cell body, causing bacterial death, thereby enhancing the photothermal effect. The chemotherapy drug DOX is controllably released under the influence of pH and NIR irradiation. DOX embeds into the DNA in the nucleus of Staphylococcus aureus, inhibiting DNA replication and transcription, thereby affecting bacterial division and achieving a synergistic antibacterial effect with the other components of the wound dressing.

[0010] The honeycomb nanofiber-based flexible probe medical dressing of the utility model can be used for the treatment and anti-infection of wounds after skin tumor resection, and can also detect the high active oxygen concentration in the wound to determine whether the wound is infected.

[0011] The utility model has the following beneficial effects:

[0012] The honeycomb-shaped nanofiber-based flexible probe medical dressing of the present invention can simultaneously monitor, treat, and combat bacterial infections. This medical dressing adopts a formatted honeycomb shape, with hexagonal branches serving as the detection portion and the hexagonal grid containing the treatment portion. The honeycomb shape effectively separates and combines the treatment and detection portions. While treating the wound, changes in the fluorescence intensity of the detection portion can be used to detect infection within the wound. The honeycomb dressing ensures that the therapeutic agent and the detection substance fit tightly together and are neatly arranged. Furthermore, due to their distinct shapes and orderly spacing, the dressing avoids the situation where the fluorescence intensity of the detection portion decreases while the treatment portion is active. Furthermore, the formatted and neat nature of the honeycomb pattern makes the size of the medical dressing highly adjustable. Furthermore, the dressing does not adhere to the wound, thereby preventing further damage to the newly formed epithelium and bleeding. The dressing can also be applied according to the shape of the wound, offering advantages such as ease of operation and rapid effectiveness. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the structure of the utility model, wherein 1 is the detection part and 2 is the treatment part. DETAILED DESCRIPTION

[0014] As attached Figure 1As shown, the utility model is a honeycomb nanofiber-based flexible probe medical dressing, which is in the shape of a honeycomb formed by closely arranged hexagonal structural units, wherein the six sides of the hexagonal structural unit are the detection part 1, and the middle surrounded part is the treatment part 2, and the detection part 1 and the treatment part 2 are tightly fitted.

[0015] The detection part 1 is used to detect the concentration of high reactive oxygen species (hROS) in the wound and determine whether the wound is infected.

[0016] The therapeutic part 2 is used for the treatment of wounds after skin tumor excision and for anti-infection.

[0017] Detection part 1 is a fluorescent probe based on gold nanoclusters stabilized by cellulose nanocrystals (CNC) (CNC@GNCs fluorescent probe), which is used to detect the concentration of highly reactive oxygen species (hROS). In the presence of highly reactive oxygen species (hROS), the blue fluorescence of the fluorescent probe is quenched.

[0018] The CNC@GNCs fluorescent probe is an existing material that can be prepared using existing technologies. The preparation method has been published in: A bioinspired ratiometric fluorescence probe based on cellulose nanocrystal-stabilized gold nanoclusters for live-cell and zebrafish imaging of highly reactive oxygen species, Chemical Engineering Journal, 2022, 431, 133954. The details are as follows:

[0019] 1) In the dark, 7.0 mL of a 1.0 mmol / L HAuCl4·3H2O solution was dissolved in 10.0 mL of the prepared CNC solution (solid content 1.4 wt%) and boiled at 110°C. Then, 1.0 mL of a 3.5 mmol / L levodopa solution was added and boiled at 110°C. After 5 minutes, the solution was cooled to room temperature. The color of the mixed solution changed from a light yellow transparent solution to a light gray, yielding cellulose nanocrystal-stabilized gold nanoclusters (CNC@GNCs). The solution was stored in borate buffer at 4°C in the dark and labeled as CNC@GNCs.

[0020] 2) Dissolve 18g of polyvinyl alcohol (PVA) in 82mL of ultrapure water and heat to 80-90°C to dissolve it, yielding an 18wt% PVA aqueous solution. Add 4mL of CNC@GNCs and stir for 1h to obtain a gel-like cellulose nanocrystal-stabilized gold nanocluster (CNC@GNCs) fluorescent probe.

[0021] Treatment component 2 is a near-infrared (NIR) and pH-responsive cellulose nanofiber nanocage wound dressing (CNFNWD@ICG&DOX). It is designed to kill residual tumor cells, resist invasion by external bacterial pathogens, and eliminate biofilms in wounds following skin tumor resection. The dual NIR and pH responses act as an "on / off" switch, enabling the controlled and effective release of the drugs doxorubicin (DOX) and indocyanine green (ICG) from the dressing into the wound area. This effectively kills residual tumor cells, resists invasion by external bacterial pathogens, and eliminates biofilms. The ICG in the dressing detaches from and enters bacterial cells, releasing reactive oxygen species (ROS) upon exposure to near-infrared laser light. ROS attack the bacterial cell body, causing bacterial death and enhancing the photothermal effect. The chemotherapy drug DOX is controllably released under the influence of pH and NIR irradiation. DOX intercalates into DNA in the nucleus of Staphylococcus aureus, inhibiting DNA replication and transcription, thereby affecting bacterial division. This synergistic antibacterial effect is achieved with the other components of the wound dressing.

[0022] Among them, CNF NWD@ICG&DOX is an existing material that can be prepared using existing technologies. Its preparation method was published in A bionic cellulose nanofiber-based nanocage wound dressing for NIR-triggered multiple synergistic therapy of tumors and infected wounds[J], Biomaterials, 2022, 281, 121330. The details are as follows:

[0023] 1) Preparation of carboxylated cellulose nanofibers (CNF)

[0024] 2.0000 g of bagasse fiber was added to 200.0 mL of deionized water and stirred thoroughly until the fibers were dispersed. 0.0320 g of TEMPO, 2.0000 g of NaBr, and 3.6 mL of NaClO were then added to the above mixture. The pH of the reaction system was adjusted to 10 with 0.5 M NaOH, and at the end of the reaction at 60 ° C, 10.0 mL of anhydrous ethanol was added to the reaction system to quench the reaction. Finally, the mixture was centrifuged and washed repeatedly with distilled water until the supernatant was neutral. The TEMPO-oxidized cellulose nanofibers were homogenized using a microfluidic generator. The 1.0 wt% TEMPO-oxidized cellulose nanofiber aqueous solution was treated in a straight tube 5 times, then circulated 6 times under 1500 bar nitrogen protection, and the final uniform suspension was then freeze-dried for 24 hours. The resulting product was carboxylated cellulose nanofibers, labeled CNF.

[0025] 2) Preparation of near-infrared and pH-responsive cellulose nanofibers

[0026] An amount of 1.0000 g of CNF was added to 300.0 mL of deionized water and sonicated for 10 minutes for uniform dispersion. Then, 1.0000 g of NHS, 1.0000 g of EDC, and 3.0000 g of DA were added to the above mixture. The mixture was magnetically stirred at 25 °C in the dark for 24 h. The resulting product was centrifuged for 10 minutes, and the precipitate was repeatedly washed with deionized water until the supernatant was neutral. After freeze-drying for 48 h, the product was near-infrared responsive cellulose nanofibers, labeled CNF-DA. pH-responsive cellulose nanofibers, labeled CNF-PEI, were synthesized using a similar method.

[0027] 3) Preparation of medical dressings and drug loading

[0028] 0.6800 g of CNF-PEI and 0.1200 g of CNF-DA were added to 50.0 mL of deionized water. The mixture was homogenized by ultrasonication for 30 minutes, followed by the addition of 1.0 mL of 2.0 wt% PVA solution and magnetic stirring at 55°C until homogeneous. The resulting product was a NIR- and pH-responsive CNF-based nanocage wound dressing, labeled CNFNWD.

[0029] DOX and ICG drug loading was performed in preparation of a wound dressing. 40.00 mg of DOX and 3.00 mg of ICG were dispersed uniformly in 1.0 mL of deionized water and then added to 9.0 mL of a dressing solution (1 wt%) under magnetic stirring for 30 minutes. The mixture was then sonicated at 4°C for 30 minutes. The resulting product was a drug-loaded, NIR- and pH-responsive, CNF-based nanocage wound dressing, labeled CNF NWD@ICG&DOX.

[0030] The specific preparation steps of the honeycomb nanofiber-based flexible probe medical dressing of the present invention are as follows:

[0031] 1) The detection component, namely the gel-like cellulose nanocrystal-stabilized gold nanoclusters (CNC@GNCs) fluorescent probe, was injected into the legs of a honeycomb hexagonal mold, with the legs having an inner diameter of 4 mm, a width of 1 mm, and a height of 1 mm. The detection mixture was spread flat on a plastic Petri dish and frozen at -20°C until frozen. The mixture was then removed from the refrigerator and thawed until melted. This process was repeated three times before removal.

[0032] 2) The therapeutic part, i.e., near-infrared (NIR) and pH dual-responsive cellulose nanofiber nanocage wound dressing (CNFNWD@ICG&DOX), was injected into the hexagonal grid of a honeycomb hexagonal mold, with the side length of the hexagonal grid controlled to be 4 mm. The mold was then frozen at -20°C in a refrigerator until ice formed, thereby obtaining a honeycomb nanofiber-based flexible probe medical dressing.

Claims

1. A honeycomb nanofiber-based flexible probe medical dressing, characterized in that: The shape of the medical dressing is a honeycomb formed by closely arranged hexagonal structural units, wherein the six sides of the hexagonal structural units are detection parts, and the middle surrounded part is the treatment part, and the detection part and the treatment part are tightly fitted; the detection part is used to detect the high reactive oxygen concentration in the wound and determine whether the wound is infected; the treatment part is used for the treatment and anti-infection of the wound after skin tumor resection; the detection part is a fluorescent probe based on gold nanoclusters stabilized by cellulose nanocrystals, which is used to detect high reactive oxygen concentration; the treatment part is a near-infrared and pH dual-responsive cellulose nanofiber nanocage wound dressing, which is used to kill residual tumor cells in the wound after skin tumor resection, resist the invasion of external bacterial pathogens and eliminate biofilm effects.

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