Blood collection card

Abstract

The utility model discloses a blood collection card, and belongs to the technical field of body fluid collection cards. Comprising a supporting clamping shell, a PVC bottom plate, a serum collecting pad, a buffering pad, a blood filtering pad and a cell blocking pad, and a mold strip is packaged in the supporting clamping shell; a collection window is arranged on the top surface of one end of the supporting clamping shell; a breaking weak part is arranged at the middle rear part of the supporting clamping shell; the die strip comprises the serum collecting pad which is adhered to the top surface of the PVC bottom plate; one end of the buffer pad is lapped to the top surface of one end of the serum collection pad; the other end of the buffer pad is adhered to the top surface of the PVC bottom plate; one end of the blood filtering pad is lapped to the top surface of one end of the buffer pad; the other end of the blood filtering pad is adhered to the top surface of the PVC bottom plate; one end of the cell blocking pad is lapped to the top surface of one end of the blood filtering pad; the other end of the cell barrier pad is adhered to the top surface of the PVC bottom plate; the blood collection card disclosed by the utility model can be used for separating serum from blood cells, and is suitable for in-vitro detection of serum markers.

Description

Technical Field

[0001] The utility model specifically relates to a blood collection card, belonging to the technical field of body fluid collection cards. Background Art

[0002] The blood sample collection card is a new medium for storing DNA with plant fiber as the carrier. It can bind DNA to the card and is designed for the collection, transportation and storage of blood samples at room temperature. Like the existing DBS card paper, blood drops are collected on the DBS card paper, dried and transported, and then the dried blood spots are extracted and analyzed in the laboratory. The soaking liquid of the collection card makes the product have the functions of protein denaturation, cell membrane rupture, DNA adsorption and fixation, and inhibition of bacterial growth, thereby ensuring that the target DNA can be stored on the collection card for many years. Due to the denaturation of the protein, the pathogenic microorganisms or viruses in the biological sample lose their activity, avoiding the risk of contamination to the operator and ensuring the safety of long-distance transportation. Patent authorization announcement number: CN222882363U, discloses a blood sample collection card, including a first paperboard, an adsorption filter paper and a second paperboard stacked in sequence, the upper surface of the second paperboard is provided with an information collection area and a sample collection area, and the adsorption filter paper is adhered to the sample collection area; a collection hole is provided on the adsorption filter paper, and a collection paper piece is provided in the collection hole, and the outer peripheral side of the collection paper piece is connected to the hole wall of the collection hole through a weak part that is easy to break; a first through hole corresponding to the collection hole is provided on the first paperboard, and the hole wall of the first through hole is located on the outer peripheral side of the hole wall of the collection hole. The blood sample collection card with this structure is convenient for sampling, and the collection paper piece can be removed by tweezers to achieve sampling; however, the collection card with this structure uses whole blood samples, which is suitable for DNA testing, but cannot separate serum or plasma. Utility Model Content

[0003] In order to solve the above problems, the utility model proposes a blood collection card, which can separate serum and blood cells and is suitable for in vitro detection of serum markers.

[0004] The blood collection card of the utility model includes a support card shell, wherein a mold strip is encapsulated in the support card shell; a collection window is provided on the top surface of one end of the support card shell; a folding weak portion is provided in the middle and rear part of the support card shell; the mold strip includes:

[0005] PVC baseboard;

[0006] A serum collection pad adhered to the top surface of the PVC base plate;

[0007] A buffer pad, one end of which is overlapped with the top surface of one end of the serum collection pad; and the other end of which is bonded to the top surface of the PVC bottom plate;

[0008] A blood filter pad, one end of which is overlapped with the top surface of one end of the buffer pad; and the other end of which is bonded to the top surface of the PVC bottom plate;

[0009] A cell barrier pad, one end of which is overlapped with the top surface of one end of the blood filter pad; and the other end of which is bonded to the top surface of the PVC bottom plate;

[0010] The cell barrier pad and the buffer pad are made of glass fiber pads, and the glass fiber pads are soaked and dried with red blood cell barrier treatment liquid; the serum collection pad is made of filter paper; and the blood filter pad is made of a blood filter membrane.

[0011] Treatment of red blood cell barrier pads: Soak glass fiber 9864 in red blood cell barrier treatment solution, which contains 3% to 10% anti-red blood cell antibodies, preservative proclin 300, and 0.1% phosphate buffer. After soaking, place in an oven for drying. After drying, cut into 10-20mm*300mm pieces, which must be parallel, neat, and without burrs, and must be completely dry and moisture-proof.

[0012] Preparation of serum collection pads: Cut filter paper into 10~30mm*300mm pieces, parallel and neat without burrs, and without wrinkles or cracks; dry in a 45℃ oven for more than 2 hours before use;

[0013] Cushion pad: The processing method is the same as that of the red blood cell barrier pad, cut into 5~10mm*300mm, and it must be parallel, neat and without burrs, completely dry and moisture-proof;

[0014] Blood filter pad: Cut the blood filter membrane into 10~20mm*300mm pieces, which should be parallel, neat and without burrs, and completely dry without moisture absorption;

[0015] The PVC base plate, serum collection pad, buffer pad, blood filter pad and cell barrier pad are assembled to obtain a mold strip, and the assembled membrane strip is cut into 4-8 mm width and installed in the support card shell, and then packaged after compaction.

[0016] Furthermore, the support card shell is encapsulated inside a self-sealing aluminum foil bag, which contains 10g / bag of desiccant. The desiccant can ensure the stability of the separated serum, and the red blood cell barrier pad treatment fluid contains a preservative to further ensure the stability of the serum sample.

[0017] Furthermore, an EP tube (centrifuge tube) is packaged inside the self-sealing aluminum foil bag; it is used to separate trace reagents; the EP tube is perfused with diluent; the diluent is soaked to extract the sample collected by the serum collection pad.

[0018] Furthermore, the buffer pad and the serum collection pad form a first overlapping area, the blood filter pad and the buffer pad form a second overlapping area, and the cell barrier pad and the blood filter pad form a third overlapping area. The length and width of the first overlapping area to the third overlapping area are both 1-5 mm; chromatography of the collected blood drops can be achieved through the first overlapping area to the third overlapping area.

[0019] Furthermore, one or more acquisition windows are provided, and a corresponding number of acquisition windows can be selected according to acquisition requirements.

[0020] Compared with the existing technology, the blood collection card of the present invention separates serum and blood cells through lateral chromatography, is capable of separating serum and blood cells, is compatible with the room temperature stability of traditional blood collection cards, and is suitable for in vitro detection of serum markers; the blood collection card adopts a shell structure, and the biological sample is placed in the card compartment supporting the card shell to avoid cross-contamination and clinical sample contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the overall structure of the blood collection card of the present utility model.

[0022] Figure 2 This is a schematic diagram of the overall structure of the mold bar of the present utility model.

[0023] Figure 3 This is a schematic diagram of the packaging structure of the blood collection card, self-sealing aluminum foil bag, desiccant and EP tube of the utility model.

[0024] Figure numerals: 1. Support card, 2. Collection window, 3. Bend weak part, 4. PVC bottom plate, 5. Serum collection pad, 6. Buffer pad, 7. Blood filter pad, 8. Cell barrier pad, 9. Self-sealing aluminum foil bag, 10. Desiccant, 11. EP tube. DETAILED DESCRIPTION

[0025] Example:

[0026] like Figures 1 to 3 The blood collection card shown in the figure includes a support card shell 1, in which a mold strip is encapsulated; a collection window 2 is provided on the top surface of one end of the support card shell 1; a folding weak portion 3 is provided in the middle and rear portion of the support card shell 1; the mold strip includes:

[0027] PVC base plate 4;

[0028] A serum collection pad 5 is bonded to the top surface of the PVC base plate 4;

[0029] A buffer pad 6, one end of which is overlapped with the top surface of one end of the serum collection pad 5; the other end of which is bonded to the top surface of the PVC bottom plate 4;

[0030] A blood filter pad 7, one end of which is overlapped with the top surface of one end of the buffer pad 6; the other end of which is bonded to the top surface of the PVC bottom plate 4;

[0031] A cell barrier pad 8, one end of which is overlapped with the top surface of one end of the blood filter pad 7; the other end of which is bonded to the top surface of the PVC bottom plate 4;

[0032] The cell barrier pad 8 and the buffer pad 6 are made of glass fiber pads, and the glass fiber pads are soaked and dried with red blood cell barrier treatment fluid; the serum collection pad 5 is made of filter paper; and the blood filter pad 7 is made of a blood filter membrane.

[0033] Treatment of red blood cell barrier pad 8: Soak glass fiber 9864 in red blood cell barrier treatment solution, which contains 3% to 10% anti-red blood cell antibodies, preservative proclin 300, and 0.1% phosphate buffer; after soaking, place in an oven to dry; after drying, cut into 10-20mm*300mm pieces, which must be parallel, neat, and without burrs, and dry completely without moisture;

[0034] Preparation of serum collection pad 5: Cut filter paper into 10~30mm*300mm pieces, parallel and neat without burrs, and without wrinkles or cracks; dry in a 45℃ oven for more than 2 hours before use;

[0035] Buffer pad 6: The processing method is the same as that of the red blood cell barrier pad 8. Cut into 5~10mm*300mm, parallel and neat without burrs, dry completely and do not absorb moisture;

[0036] Blood filter pad 7: Cut the blood filter membrane into 10~20mm*300mm pieces, which should be parallel, neat and without burrs, and completely dry without moisture absorption;

[0037] The PVC base plate 4, serum collection pad 5, buffer pad 6, blood filter pad 7 and cell barrier pad 8 are assembled to obtain a mold strip, and the assembled membrane strip is cut into 4-8 mm width and installed in the support card shell 1, and then packaged after compaction.

[0038] The support card shell 1 is encapsulated inside a self-sealing aluminum foil bag 9, which contains a 10g / bag desiccant 10. The desiccant 10 can ensure the stability of the separated serum, and the red blood cell barrier pad 8 processing fluid contains a preservative to further ensure the stability of the serum sample.

[0039] The self-sealing aluminum foil bag 9 further encapsulates an EP tube 11 , which is an existing small centrifuge tube that can be used with a microcentrifuge. The EP tube 11 is filled with a diluent; the diluent is soaked to extract the sample collected by the serum collection pad 5 .

[0040] The buffer pad 6 and the serum collection pad 5 form a first overlapping area, the blood filter pad 7 and the buffer pad 6 form a second overlapping area, and the cell barrier pad 8 and the blood filter pad 7 form a third overlapping area. The length and width of the first overlapping area to the third overlapping area are both 1-5 mm; chromatography of the collected blood drops can be achieved through the first overlapping area to the third overlapping area.

[0041] There are one or more acquisition windows 2, and a corresponding number of acquisition windows 2 can be selected according to acquisition requirements.

[0042] When using, the steps for using the blood collection card are as follows:

[0043] 1. Blood collection:

[0044] (1) Take a blood collection card, a blood collection needle, an alcohol cotton pad, and a saline dropper; place the blood collection card on a horizontal table;

[0045] (2) Clean and dry your hands thoroughly, warm and massage your fingers to promote blood circulation, open the protective cap of the lancet, align the cross section of the lancet with the blood collection site, and press the launch button;

[0046] (3) Use an alcohol pad to wipe off the first drop of blood squeezed out from the blood collection site, squeeze out the peripheral blood again, and let two drops drip naturally onto the collection window 2;

[0047] (4) Add blood to the blood collection card, wait 5-10 minutes, and add 2 drops of normal saline;

[0048] (5) Place the blood collection card after adding normal saline on a horizontal table and let it dry in the shade for more than 1 hour. Do not move it during this process and avoid it near heat sources.

[0049] (6) Put the blood collection card carrying the sample back into the self-sealing aluminum foil bag 9, put the blood collection card back into the outer packaging bag, and send it to the inspection office for testing;

[0050] 2. Serum recovery:

[0051] (1) Open the self-sealing aluminum foil bag 9 and take out the blood collection card carrying the sample;

[0052] (2) Slightly bend the two ends of the support card shell 1 of the blood collection card, open the support card shell 1 along the folded weak portion 3, and remove the internal mold strip;

[0053] (3) Bend the two ends of the mold backward and remove the serum collection pad 5 with the sample in the middle of the mold;

[0054] (4) Confirm whether there is hemolysis on the serum collection pad 5, and trim the filter paper strip with hemolysis before use;

[0055] (5) Place the serum collection pad 5 into the EP tube 11 containing the physiological saline and sample diluent provided with the blood collection card, and use a homogenizer at a speed of 1000 rpm to mix thoroughly for 20-30 minutes;

[0056] (6) The physiological saline and sample diluent soaked in the serum collection pad 5 are centrifuged at low speed and then recovered, and the samples are directly added to the test using the reagent cards for different test items.

[0057] The above embodiments are only preferred implementations of the present invention. Therefore, any equivalent changes or modifications made according to the structure, features and principles described in the scope of application of the present invention are included in the scope of application of the present invention.

Claims

1. A blood collection card, characterized in that: It includes a supporting card shell, wherein the supporting card shell encapsulates a mold strip; A collection window is provided on the top surface of one end of the support housing; The support housing is provided with a folding weak portion at the rear; the mold strip comprises: PVC baseboard; A serum collection pad adhered to the top surface of the PVC base plate; A buffer pad, one end of which is overlapped with the top surface of one end of the serum collection pad; and the other end of which is bonded to the top surface of the PVC bottom plate; A blood filter pad, one end of which is overlapped with the top surface of one end of the buffer pad; and the other end of which is bonded to the top surface of the PVC bottom plate; A cell barrier pad, one end of which is overlapped with the top surface of one end of the blood filter pad; and the other end of which is bonded to the top surface of the PVC bottom plate; The cell barrier pad and the buffer pad are made of glass fiber pads, and the glass fiber pads are soaked and dried with red blood cell barrier treatment liquid; the serum collection pad is made of filter paper; and the blood filter pad is made of a blood filter membrane.

2. The blood collection card according to claim 1, characterized in that: The support card shell is packaged inside a self-sealing aluminum foil bag, and the self-sealing aluminum foil bag contains 10g / bag of desiccant.

3. The blood collection card according to claim 2, wherein: An EP tube is also packaged inside the self-sealing aluminum foil bag, and a diluent is poured into the EP tube.

4. The blood collection card according to claim 1, wherein: The buffer pad and the serum collection pad form a first overlapping area, the blood filter pad and the buffer pad form a second overlapping area, and the cell barrier pad and the blood filter pad form a third overlapping area. The length and width of the first overlapping area to the third overlapping area are both 1-5 mm.

5. The blood collection card according to claim 1, wherein: There are one or more collection windows.

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