PCR (Polymerase Chain Reaction) tube and fluorescent quantitative PCR instrument

Abstract

The utility model discloses a PCR (Polymerase Chain Reaction) tube and a fluorescent quantitative PCR instrument. The PCR tube comprises a dustproof plug, a temperature control tube and a tube body for containing a detection reagent, a first opening is formed in the upper end of the tube body; the dustproof plug is arranged on the periphery of the temperature control pipe in a surrounding mode, and the dustproof plug and the temperature control pipe are arranged in the first opening and contained in the pipe body. The temperature control pipe is provided with a liquid conveying pipeline and a liquid discharging pipeline; the liquid conveying pipeline is used for introducing a heat-conducting medium, and the liquid discharging pipe is used for discharging the heat-conducting medium, so that the temperature control pipe can heat or cool a detection reagent in the pipe body. According to the technical scheme, the reaction time of the detection reagent is shortened.

Description

Technical Field

[0001] The utility model relates to the technical field of gene detection kits, in particular to a PCR tube and a fluorescent quantitative PCR instrument. Background Art

[0002] The ADH1B and ALDH2 genes play a key role in alcohol metabolism in the human body. They influence an individual's tolerance and metabolic rate for alcohol. Differences in their genotypes can lead to differences in the efficiency of alcohol metabolism pathways, which in turn affects an individual's tolerance for alcohol, the health risks of drinking behavior, and the risk of alcohol-related diseases. Existing methods utilize PCR TaqMan probes to detect single-base mutations in the ADH1B and ALDH2 genes. However, these reagents suffer from weak anti-interference capabilities, and test samples require DNA extraction and purification. This leads to a series of problems with ADH1B and ALDH2 gene testing, including numerous steps, high costs, and long reaction times. Therefore, on-site testing is not feasible. Existing methods also propose the use of tongue swabs for detecting ADH1B and ALDH2 genes. While this method does not require DNA extraction and purification and can be performed on-site, its reaction time is still relatively long. Utility Model Content

[0003] The purpose of the utility model is to provide a PCR tube, aiming to shorten the reaction time of detection reagents.

[0004] To achieve this purpose, the present invention adopts the following technical solutions:

[0005] A PCR tube, comprising: a dust plug, a temperature control tube, and a tube body for containing a detection reagent;

[0006] The upper end of the tube body has a first opening;

[0007] The dust plug ring is arranged on the outer periphery of the temperature control tube, the dust plug and the temperature control tube are arranged at the first opening and accommodated inside the tube body;

[0008] The temperature control tube has an infusion pipe and a drainage pipe; the infusion pipe is used to introduce a heat-conducting medium, and the drainage pipe is used to discharge the heat-conducting medium, so that the temperature control tube can heat or cool the detection reagent in the tube body.

[0009] In one embodiment, the wall of the temperature control tube is sprayed with an opaque layer.

[0010] In one embodiment, a connecting piece is further included, wherein the connecting piece has two installation positions and is used to install the two tube bodies respectively.

[0011] In one embodiment, the lower end of the tube is tapered.

[0012] In one embodiment, the dust plug and the temperature control tube are formed separately;

[0013] The middle part of the dust plug is further provided with a tearing layer, and under the action of an external force, the tearing layer can be torn open so that the temperature control tube is located in the middle part of the dust plug.

[0014] The present invention also provides a fluorescent quantitative PCR instrument, comprising any one of the above-mentioned PCR tubes;

[0015] The fluorescent quantitative PCR instrument also includes a heating device, a cooling device, a liquid collection tank, a first pump body, and a second pump body. The drainage pipeline, the first pump body, the liquid collection tank, the heating device, the second pump body, and the liquid delivery pipeline are sequentially connected; the cooling device is connected in parallel to the input and output ends of the heating device. Compared with the existing technology, the present invention has the following advantages:

[0016] Existing fluorescence quantitative PCR instruments usually need to be heated to a certain temperature when performing fluorescence quantitative detection on the detection reagents in the PCR tube. The detection reagents are detected and analyzed using the principles of high-temperature deformation, low-temperature renaturation, and suitable temperature extension. Usually, when the PCR tube is heated, it needs to be heated from the outside and naturally cooled, so the temperature change time is slow, wasting a lot of detection time. In the technical solution of the present utility model, a PCR tube is proposed. By setting a temperature control tube for the PCR tube, the heat-conducting medium in the temperature control tube is used to heat or cool the detection reagent in the tube body, thereby achieving simultaneous heating and cooling of the inside and outside of the detection reagent, accelerating the temperature change time, and thus accelerating the reaction time of the detection reagent.

[0017] Furthermore, the technical solution of the present invention utilizes a heat-conducting medium to gently heat the reagent in the tube body, thereby ensuring the activity of the detection reagent and further ensuring the stability of the detection reagent. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0019] The structures, proportions, sizes, etc. depicted in the drawings of this specification are only used to match the contents disclosed in this specification so as to facilitate understanding and reading by those familiar with this technology. They are not intended to limit the conditions under which the present invention can be implemented, and therefore have no substantive technical significance. Any structural modifications, changes in proportional relationships, or adjustments in size, without affecting the efficacy and objectives that can be achieved by the present invention, should still fall within the scope of the technical contents disclosed in the present invention.

[0020] Figure 1 This is a schematic structural diagram of an embodiment of the present utility model;

[0021] Figure 2 A cross-sectional view of an embodiment of the present invention;

[0022] Figure 3 This is a schematic diagram of an embodiment of the present utility model;

[0023] Illustrations: 100, PCR tube; 110, dust plug; 120, temperature control tube; 121, infusion pipe; 122, drainage pipe; 130, tube body; 131, first opening; 140, connector;

[0024] 20. Fluorescence quantitative PCR instrument; 21. Heating device; 22. Cooling device; 23. Liquid collecting tank; 24. First pump body; 25. Second pump body; 26. Thermometer; 27. Solenoid valve. DETAILED DESCRIPTION

[0025] In order to make the technical objectives, features, and advantages of the present invention more obvious and easy to understand, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the embodiments described below are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0026] In the description of the present invention, it should be understood that the terms "upper," "lower," "top," "bottom," "inner," "outer," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate the description of the present invention and simplify the description. They are not intended to indicate or imply that the devices or elements referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on the present invention. It should be noted that when a component is considered to be "connected" to another component, it may be directly connected to the other component or there may be a centrally located component.

[0027] The technical solution of the present invention will be further described below with reference to the accompanying drawings and through specific implementation methods.

[0028] An embodiment of the present invention provides a PCR tube 100 .

[0029] like Figure 1 and Figure 2 As shown, the PCR tube 100 comprises: a dust plug 110, a temperature control tube 120 and a tube body 130 for containing detection reagents;

[0030] The upper end of the tube body 130 has a first opening 131;

[0031] The dust plug 110 is disposed around the outer circumference of the temperature control tube 120 . The dust plug 110 and the temperature control tube 120 are disposed at the first opening 131 and are housed inside the tube body 130 .

[0032] The temperature control tube 120 has an infusion pipe 121 and a drainage pipe 122 ; the infusion pipe 121 is used to introduce a heat-conducting medium, and the drainage pipe is used to discharge the heat-conducting medium, so that the temperature control tube 120 can heat or cool the detection reagent in the tube body 130 .

[0033] It is understandable that the fluorescent quantitative PCR instrument 20 usually needs to heat the detection reagent in the PCR100 tube to a certain temperature when detecting it. The detection reagent is detected and analyzed using the principles of high-temperature deformation, low-temperature annealing, and suitable temperature extension. Usually, when the PCR tube is heated, it needs to be heated from the outside and naturally cooled, so the temperature change time is slow, wasting a lot of detection time. In the technical solution of the present utility model, a PCR tube 100 is proposed. By setting a temperature control tube 120 for the PCR tube, the heat-conducting medium in the temperature control tube 120 is used to heat or cool the detection reagent in the tube body 130, thereby achieving simultaneous heating and cooling of the inside and outside of the detection reagent, accelerating the temperature change time, and thus accelerating the reaction time of the detection reagent.

[0034] Furthermore, the technical solution of the present invention utilizes a heat-conducting medium to gently heat the reagent in the tube body 130 , thereby ensuring the activity of the detection reagent and further ensuring the stability of the detection reagent.

[0035] Optionally, the heat-conducting medium is water, or a liquid with water as a solute.

[0036] In one embodiment, in order to enhance the stability of the fluorescent signal and avoid misidentification of the heat-conducting medium in the temperature control tube 120 , the tube wall of the temperature control tube 120 is sprayed with an opaque layer.

[0037] Optionally, the opaque layer is a metal layer.

[0038] Optionally, the metal layer is coated on the inner arm of the temperature control tube 120 .

[0039] like Figure 1 and Figure 2 As shown, in a specific embodiment, the PCR tube 100 further includes a connector 140 , and the connector 140 has two mounting positions and is used to mount the two tube bodies 130 respectively.

[0040] It is understood that when performing ADH1B gene testing, the ALDH2 gene is usually also tested to ensure the accuracy of the test results. Therefore, using the connector 140, the two test reagent tubes 130 are combined as a group of test samples for simultaneous testing, which improves the efficiency of single-group sample testing and saves patients waiting time.

[0041] like Figure 2 As shown, in a specific embodiment, in order to increase the heating rate of the detection reagent in the tube body 130, the lower end of the tube body 130 is tapered. The tapered design increases the contact area between the detection reagent and the tube body 130, thereby increasing the natural cooling rate of the detection reagent and the heating rate when heated from the outside.

[0042] Optionally, the dust plug 110 and the temperature control tube 120 are formed separately;

[0043] A tearing layer is further provided in the middle of the dust plug 110 . Under the action of an external force, the tearing layer can be torn open so that the temperature control tube 120 is located in the middle of the dust plug 110 .

[0044] Optionally, the dust plug 110 and the temperature control tube 120 are integrally formed.

[0045] The technical solution of the present utility model further provides a fluorescent quantitative PCR instrument 20, comprising any one of the above-mentioned PCR tubes 100;

[0046] The fluorescent quantitative PCR instrument 20 also includes a heating device 21, a cooling device 22, a liquid collecting tank 23, a first pump body 24 and a second pump body 25. The drainage pipe 122, the first pump body 24, the liquid collecting tank 23, the heating device 21, the second pump body 25 and the infusion pipe 121 are connected in sequence; the cooling device 22 is connected in parallel to the input and output ends of the heating device 21.

[0047] It is understood that the heating device 21 is used to heat the heat-conducting medium flowing out of the liquid collection tank 23, ensuring that the heat-conducting medium flowing out of the liquid infusion pipe 121 can assist in heating the detection reagent; the cooling device 22 is used to cool the heat-conducting medium flowing out of the liquid collection tank 23, ensuring that the heat-conducting medium flowing out of the liquid infusion pipe 121 can assist in cooling the detection reagent. The liquid collection tank 23 is used to store the heat-conducting medium; the first pump body 24 and the second pump body 25 are used to drive the circulation of the heat-conducting medium in the discharge pipe 122 and the liquid infusion pipe 121.

[0048] Optionally, the fluorescent quantitative PCR instrument 20 further includes a thermometer 26, which is provided in the output pipeline of the heating device 21 and / or the cooling device 22. The thermometer 26 is used to detect the temperature of the heat-conducting medium in the pipeline.

[0049] Optionally, the fluorescence quantitative PCR instrument 20 further includes a solenoid valve 27 , which is provided in the input pipeline of the heating device 21 and / or the cooling device 22 to control the on-off of the input pipeline.

[0050] As described above, the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A PCR tube, characterized in that: include: Dust plugs, temperature control tubes, and tubes for containing test reagents; The upper end of the tube body has a first opening; The dust plug ring is arranged on the outer periphery of the temperature control tube, the dust plug and the temperature control tube are arranged at the first opening and accommodated inside the tube body; The temperature control tube has an infusion pipe and a drainage pipe; the infusion pipe is used to introduce a heat-conducting medium, and the drainage pipe is used to discharge the heat-conducting medium, so that the temperature control tube can heat or cool the detection reagent in the tube body.

2. The PCR tube according to claim 1, characterized in that The wall of the temperature control tube is sprayed with a light-proof layer.

3. The PCR tube according to claim 1, characterized in that It also includes a connecting piece, which has two installation positions and is used to install the two pipe bodies respectively.

4. The PCR tube according to claim 1, characterized in that The lower end of the tube body is tapered.

5. The PCR tube according to claim 1, characterized in that The dust plug and the temperature control tube are formed separately; The middle part of the dust plug is further provided with a tearing layer, and under the action of an external force, the tearing layer can be torn open so that the temperature control tube is located in the middle part of the dust plug.

6. A fluorescence quantitative PCR instrument, characterized in that: A PCR tube comprising the PCR tube according to any one of claims 1 to 5; The fluorescent quantitative PCR instrument also includes a heating device, a cooling device, a liquid collecting tank, a first pump body and a second pump body. The drainage pipe, the first pump body, the liquid collecting tank, the heating device, the second pump body and the infusion pipe are connected in sequence; the cooling device is connected in parallel to the input and output ends of the heating device.

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