Continuous tubular reaction device for concerted catalysis of enzyme and immobilized enzyme

Abstract

The utility model discloses a continuous tubular reaction device for concerted catalysis of enzyme and immobilized enzyme, which comprises a tube body, two ends of the tube body are provided with sealing plugs, rotating motors are arranged in the sealing plugs, a rotating shaft is arranged between the rotating motors for connection, an arc-shaped tube is arranged above the tube body, and the arc-shaped tube is connected with pipeline walls on the upper sides of the two ends of the tube body. An enzyme isolation box is connected to the pipeline wall of the side face of one end of the pipe body, an enzyme separation box is arranged below the bottom side of the other end of the pipe body, an expansion pipe is arranged in the middle of the pipe body, a load rotating disc is arranged at the position, corresponding to the expansion pipe, of the rotating shaft, and a feeding box is arranged in the middle of the arc-shaped pipe. The device disclosed by the utility model has the beneficial effects that efficient concerted catalysis of enzyme and immobilized enzyme can be realized, and the catalysis efficiency is improved; by optimizing the combination mode and reaction conditions of the enzyme and the immobilized enzyme, the stability of the catalyst is improved, the failure and degradation of the catalyst are reduced, and the service life of the reactor is prolonged; the continuous tubular reaction device can be continuously operated, so that the need of frequently replacing reaction batches is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field related to tubular reaction devices, in particular to a continuous tubular reaction device for synergistic catalysis of enzymes and immobilized enzymes. Background Art

[0002] In the field of biocatalysis, enzymes are widely used as efficient and highly selective catalysts in various chemical reactions. To improve enzyme stability, reusability, and catalytic efficiency, immobilized enzyme technology has been proposed and widely used. Immobilized enzymes not only reduce catalyst loss but also maintain their activity during continuous reactions, significantly improving production efficiency and economics.

[0003] However, in traditional immobilized enzyme reactors, problems such as insufficient catalytic efficiency, poor fluidity of immobilized enzymes, long reaction times, and difficulty in separating reaction products are often encountered. Therefore, the development of a continuous tubular reactor that can solve these problems has become a research focus. Existing technologies are often unable to effectively combine enzymes and immobilized enzymes for synergistic catalysis to achieve a more efficient continuous reaction process. Therefore, there is an urgent need for a new type of reaction device that can give full play to the synergistic catalytic effect of enzymes and immobilized enzymes to improve the overall efficiency of the reaction and product quality. Utility Model Content

[0004] In view of the above shortcomings, the present invention adopts the following technical solutions:

[0005] A continuous tubular reaction device for the coordinated catalysis of enzymes and immobilized enzymes comprises a tube body, sealing plugs are provided at both ends of the tube body, a rotating motor is provided in the sealing plug, the rotating motors are connected by a rotating shaft, an arc tube is provided above the tube body, the arc tube is connected to the upper side pipe walls at both ends of the tube body, an enzyme isolation box is connected to the side pipe wall of one end of the tube body, an enzyme separation box is provided below the bottom side of the other end of the tube body, an expansion tube is provided in the middle of the tube body, a load turntable is provided at a position corresponding to the expansion tube on the rotating shaft, and a feed box is provided in the middle of the arc tube.

[0006] Furthermore, the middle portion of the expansion tube is column-shaped, and both ends are cone-shaped, and the column-shaped structure of the middle portion of the expansion tube corresponds to the load turntable.

[0007] Furthermore, a loading plate is provided in the middle of the loading turntable, and stirring blades are provided on both sides of the loading plate.

[0008] Furthermore, a mixing pipe is provided on the outside of the enzyme isolation box, the mixing pipe is connected to several feed pipes, a spiral channel is provided in the mixing pipe, a discharge pipe is provided at the bottom of the enzyme separation box, and flow valves are provided on the discharge pipe and the feed pipe.

[0009] Furthermore, a pipe below the columnar structure in the middle of the expansion pipe is connected to a sedimentation tank, a waste discharge pipe is provided at the bottom of the sedimentation tank, and a valve is provided on the waste discharge pipe.

[0010] Furthermore, the enzyme isolation box and the enzyme separation box are both provided with grooves, the grooves are both provided with sealing plates, and the sealing plates are both provided with enzyme filtration membranes at positions corresponding to the pipelines.

[0011] Furthermore, the feed box is provided with a feeding pipe, and the feeding pipe is provided with a flow valve.

[0012] The beneficial effects of the present invention are: 1. The device of the present invention can achieve efficient synergistic catalysis of enzymes and immobilized enzymes, thereby improving catalytic efficiency; 2. The device improves the stability of the catalyst and reduces the failure and degradation of the catalyst by optimizing the combination mode and reaction conditions of the enzyme and the immobilized enzyme, thereby extending the service life of the reactor; 3. The present continuous tubular reaction device can achieve automated continuous operation, reducing the need for frequent replacement of reaction batches; 4. The design of the device of the present invention takes into account the uniformity of fluid flow and the sufficient mixing of reactants, and can achieve optimal reaction conditions during the continuous reaction process; 5. By improving the reusability of the enzyme and reducing the loss of the catalyst, the device reduces the catalyst cost in the production process. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0014] Figure 2 This is a schematic diagram of the internal structure of the utility model;

[0015] Figure 3 This is a schematic diagram of the load turntable structure of the utility model.

[0016] In the figure: 1-tube body, 2-sealing plug, 3-rotating motor, 4-rotating shaft, 5-arc tube, 6-enzyme isolation box, 7-enzyme separation box, 8-expansion tube, 9-load turntable, 10-feed box, 11-load plate, 12-stirring blade, 13-mixing pipe, 14-feed pipe, 15-discharge pipe, 16-sedimentation tank, 17-waste pipe, 18-sealing plate, 19-enzyme filter membrane, 20-feeding pipe, 21-circulation pump. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0018] Combine Figures 1 to 3 Shown:

[0019] A continuous tubular reaction device for the coordinated catalysis of enzymes and immobilized enzymes comprises a tube body 1, sealing plugs 2 are provided at both ends of the tube body 1 for ensuring the sealing of both ends of the tube body 1, a rotating motor 3 is provided in the sealing plug 2 for providing rotational power, a rotating shaft 4 is provided between the rotating motors 3, an enzyme isolation box 6 is connected to the side pipe wall of one end of the tube body 1 for isolating the enzyme and preventing the enzyme from flowing into the pipe at the feed point, a mixing pipe 13 is provided on the outside of the enzyme isolation box 6, the mixing pipe 13 is connected to several feed pipes 14, a spiral channel is provided in the mixing pipe 13 for increasing the uniformity and stability of mixing, an enzyme separation box 7 is provided below the bottom side of the other end of the tube body 1 for filtering the enzyme and preventing the enzyme from being lost together with the product, a discharge pipe 15 is provided at the bottom of the enzyme separation box 7, flow valves are provided on the discharge pipe 15 and the feed pipe 14, grooves are provided in the enzyme isolation box 6 and the enzyme separation box 7, sealing plates 18 are provided in the grooves, enzyme filter membranes 19 are provided on the sealing plate 18 at positions corresponding to the pipes, so as to accurately screen and filter the enzyme to prevent it from mixing with the product.

[0020] An expansion tube 8 is provided in the middle of the tube body 1, and a loading turntable 9 is provided on the rotating shaft 4 at a position corresponding to the expansion tube 8, which is used to load the immobilized enzyme. The middle part of the expansion tube 8 is columnar and the two ends are conical. The columnar structure in the middle of the expansion tube 8 corresponds to the loading turntable 9. A loading disk 11 is provided in the middle of the loading turntable 9, which is the main body for loading the immobilized enzyme. Stirring blades 12 are symmetrically provided on both sides of the loading disk 11. The stirring blades 12 rotate in the same direction to facilitate the contact of the immobilized enzyme and promote the flow of the liquid. They are used to stir the reaction liquid in the tube body 1 and increase the contact with the immobilized enzyme. A pipeline below the columnar structure in the middle of the expansion tube 8 is connected to a sedimentation tank 16. A waste pipe 17 is provided at the bottom of the sedimentation tank 16. A valve is provided on the waste pipe 17 for discharging waste inactivated precipitates in the reaction liquid.

[0021] An arc-shaped tube 5 is provided above the tube body 1, and the arc-shaped tube 5 connects the upper pipe walls at both ends of the tube body 1. A feed box 10 is provided in the middle of the arc-shaped tube 5, and a feeding pipe 20 is provided on the feed box 10 for adding enzyme solution to prevent the inactivated enzyme from affecting the catalytic effect of the reaction solution. A flow valve is provided on the feeding pipe 20 for controlling the amount of addition. A circulation pump 21 is provided on the arc-shaped tube 5 for circulating the enzyme solution to prevent the enzyme from being deposited at the end of the tube body 1.

[0022] Working principle: The reactant solution flows into the mixing pipe through the feed pipe to mix, and then flows into the pipe body. The pipe body and the mixing pipe are isolated by an enzyme isolation box to prevent the enzyme solution from flowing back into the pipe. The reaction occurs under the action of the enzyme solution in the tank and the immobilized enzyme loaded on the load turntable. The stirring of the stirring blade transfers the precipitate to the sedimentation tank for easy removal, while ensuring full contact between the immobilized enzyme and the reaction liquid. Finally, the reaction liquid is filtered through the enzyme filter membrane and separated and discharged from the discharge pipe. The presence of the arc pipe and the circulation pump allows the enzyme to be transported from the tail end of the pipe body to the head end, ensuring the uniformity of the enzyme solution from front to back. At the same time, by arranging a suitable detection device in the pipe body, the concentration of the enzyme solution can be judged, which is convenient for controlling the addition flow of the enzyme solution through the feed box and the feeding pipe, further ensuring the stability of the reaction environment.

[0023] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the present invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be encompassed within the present invention. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

[0024] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A continuous tubular reactor for synergistic catalysis of enzymes and immobilized enzymes, characterized by: The invention comprises a tube body (1), wherein sealing plugs (2) are provided at both ends of the tube body (1), a rotating motor (3) is provided in the sealing plug (2), and a rotating shaft (4) is provided between the rotating motors (3) and connected to each other, an arc tube (5) is provided above the tube body (1), and the arc tube (5) is connected to the upper pipe walls at both ends of the tube body (1), an enzyme isolation box (6) is connected to the side pipe wall of one end of the tube body (1), and an enzyme separation box (7) is provided below the bottom side of the other end of the tube body (1), an expansion tube (8) is provided in the middle of the tube body (1), a load turntable (9) is provided on the rotating shaft (4) at a position corresponding to the expansion tube (8), a feed box (10) is provided in the middle of the arc tube (5), and a circulation pump (21) is provided on the arc tube (5).

2. A continuous tubular reaction device for synergistic catalysis of enzyme and immobilized enzyme according to claim 1, characterized in that: The expansion tube (8) has a columnar shape in the middle and cone shapes at both ends. The columnar structure in the middle of the expansion tube (8) corresponds to the load turntable (9).

3. The continuous tubular reactor for synergistic catalysis of enzyme and immobilized enzyme according to claim 1, characterized in that: A loading disc (11) is provided in the middle of the loading turntable (9), and stirring blades (12) are provided on both sides of the loading disc (11).

4. The continuous tubular reactor for synergistic catalysis of enzyme and immobilized enzyme according to claim 1, characterized in that: A mixing pipe (13) is provided on the outside of the enzyme isolation box (6), the mixing pipe (13) is connected to a plurality of feed pipes (14), a spiral channel is provided in the mixing pipe (13), a discharge pipe (15) is provided at the bottom of the enzyme separation box (7), and flow valves are provided on both the discharge pipe (15) and the feed pipe (14).

5. The continuous tubular reaction device for synergistic catalysis of enzyme and immobilized enzyme according to claim 1, characterized in that: A pipe below the columnar structure in the middle of the expansion pipe (8) is connected to a sedimentation tank (16), a waste discharge pipe (17) is provided at the bottom of the sedimentation tank (16), and a valve is provided on the waste discharge pipe (17).

6. The continuous tubular reactor for synergistic catalysis of enzyme and immobilized enzyme according to claim 1, characterized in that: The enzyme isolation box (6) and the enzyme separation box (7) are both provided with grooves, the grooves are both provided with sealing plates (18), and the sealing plates (18) are both provided with enzyme filter membranes (19) at positions corresponding to the pipelines.

7. The continuous tubular reaction device for synergistic catalysis of enzyme and immobilized enzyme according to claim 1, characterized in that: The feed box (10) is provided with a feeding pipe (20), and the feeding pipe (20) is provided with a flow valve.

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