System for realizing online circulating flow and fermentation of amino acid by adopting ceramic membrane

Abstract

The utility model discloses a system for realizing online circulating flow and fermentation of amino acid by adopting a ceramic membrane, which comprises a strain tank, a seed tank arranged on one side of the strain tank, a fermentation tank arranged on one side of the seed tank, a feed pump arranged at the top end of one side of the fermentation tank, a cleaning tank arranged on one side of the fermentation tank, and a circulating pump arranged at the bottom end of one side of the cleaning tank. According to the amino acid on-line circulating flow and fermentation system realized by adopting the ceramic membrane, the culture times of early-stage strain culture and seed tank culture are obviously reduced; the fermentation level is always in a high level state, the acid production rate of thalli is high, the yield is remarkably improved under the condition that equipment is not increased, and the total yield even can reach 2-3 times of the original yield; nutrient substances in the ceramic membrane filtrate can be sterilized after being extracted to serve as nutrient substances to be fed into the fermentation tank, so that the product output ratio of feeding is increased, namely the acid yield is increased; and the ceramic membrane can be sterilized by steam, so that the requirement of sterile equipment is met.

Description

Technical Field

[0001] The utility model relates to the technical field of amino acid fermentation, in particular to an amino acid online circulation flow and fermentation system using a ceramic membrane. Background Art

[0002] Amino acid fermentation is a biochemical process in which microorganisms decompose and metabolize nutrients and synthesize the desired products during their life activities. This method can not only produce a variety of essential amino acids, such as L-glutamic acid, L-lysine, L-ornithine, and L-valine, but the amino acids produced by fermentation can basically meet the needs of multiple fields such as food, medicine, cosmetics, and animal feed. In addition, with the application of gene editing technology and synthetic biology, the efficiency and purity of amino acid production by fermentation have been significantly improved, making this method increasingly advantageous in industrial production. The industrial applications of amino acid fermentation include not only the direct production of amino acids, but also the application of microorganisms produced by fermentation in the pharmaceutical and food industries. For example, some microorganisms can secrete alanine, glutamic acid, aspartic acid, and phenylalanine, which are widely used in medicine and food. In addition, production through genetically modified Escherichia coli and Corynebacterium glutamicum not only improves yield but also obtains safety certification, further demonstrating the importance and advantages of amino acid fermentation technology in industrial applications.

[0003] However, the traditional fermentation process has the following disadvantages:

[0004] In amino acid fermentation, batch fermentation is generally adopted, that is, one-time feeding and fermentation. During the fermentation process, carbon source is supplemented and pH value is adjusted until fermentation is stopped after a certain period, sterilized and placed in the tank. At this time, the fermentation product reaches a certain concentration, which is the maximum yield of a single batch. Because the high concentration of product at this time inhibits the progress of fermentation, and the bacteria are also accelerating aging. Although we can increase the concentration of the product by transforming the bacteria through engineering bacteria technology, this is very limited, and each batch inevitably has excessive feeding. Finally, there are still a lot of nutrients in the fermentation liquid that are not utilized by the bacteria, that is, the acid production rate is relatively low. This part of the nutrients is best treated as waste and cannot be further utilized. Each batch of fermentation must start with strain cultivation, from strain cultivation to several seed tank cultivation before it can be inoculated into a large fermentation tank for fermentation. The fermentation material and time costs of a single batch are relatively high. Utility Model Content

[0005] The purpose of the present utility model is to provide an amino acid online circulation flow and fermentation system using a ceramic membrane to solve the problem raised in the above background technology that amino acid fermentation generally adopts batch fermentation, that is, one-time feeding fermentation, carbon source supplementation and pH adjustment during the fermentation process until the fermentation reaches a certain period and stops, sterilizes and releases the tank. At this time, the fermentation product reaches a certain concentration, that is, the maximum yield of a single batch. Because the high concentration of the product inhibits the progress of fermentation, and the bacteria also accelerates aging, we can improve the concentration of the product by modifying the bacteria through engineering bacteria technology, but this is very limited, and each batch inevitably has excessive feeding. Finally, there are a large amount of nutrients in the fermentation liquid that are not utilized by the bacteria, that is, the acid production rate is relatively low. These nutrients are best treated as waste and cannot be further utilized. Each batch of fermentation must start with strain cultivation, from strain cultivation to several seed tank cultures before inoculation into a large fermentation tank for fermentation, and the fermentation material and time costs of a single batch are high.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solutions: a system for realizing online circulation flow and fermentation of amino acids using ceramic membranes, comprising a seed tank, a seed tank is provided on one side of the seed tank, a fermentation tank is provided on one side of the fermentation tank, a feeding pump is provided on the top of one side of the fermentation tank, a cleaning tank is provided on one side of the fermentation tank, a feeding tank is provided in the middle of one side of the fermentation tank, a circulation pump is provided on the bottom end of one side of the cleaning tank, a ceramic membrane system is provided on one side of the cleaning tank, a refining and purification tank is provided on one side of the ceramic membrane system, a first inoculation pipe is fixedly connected to one side of the seed tank, an end of the first inoculation pipe away from the seed tank is fixedly connected to a side opposite to the seed tank, a second inoculation pipe is fixedly connected to the side of the seed tank away from the seed tank, a side of the second inoculation pipe is fixedly connected to a side opposite to the fermentation tank, after the bacteria in the seed tank is cultured, it is transported to the seed tank through the first inoculation pipe, and the seeds in the seed tank are transported to the fermentation tank through the second inoculation pipe.

[0007] Preferably, one side of the top of the feeding tank is fixedly connected to a feeding pipe, the other side of the top of the feeding tank is fixedly connected to a nutrient solution sterilization return pipe, the end of the nutrient solution sterilization return pipe away from the feeding tank is fixedly connected to the side opposite to the refining and purification tank, the liquid inlet of the feeding pump is fixedly connected to a first extraction pipe extending to the interior of the feeding tank, and the liquid outlet of the feeding pump is fixedly connected to a first delivery pipe extending to the interior of the fermentation tank. The feeding pump is started after being powered on, and the feeding pump extracts the product in the feeding tank through the first extraction pipe, and the extracted product is delivered to the fermentation tank through the first delivery pipe.

[0008] Preferably, the liquid outlet of the circulation pump is fixedly connected to a second extraction pipe extending to the interior of the fermentation tank, the liquid inlet of the circulation pump is fixedly connected to a second delivery pipe extending to the interior of the ceramic membrane system, a first valve body is fixedly installed in the middle of the second extraction pipe, a first pressure gauge is fixedly installed in the middle of the second delivery pipe, a filter is fixedly installed inside the second extraction pipe, the circulation pump extracts the product in the ceramic membrane system through the second delivery pipe, the extracted product is delivered to the fermentation tank through the second extraction pipe, and the first pressure gauge displays the pressure of the second delivery pipe in real time.

[0009] Preferably, the bottom end of the cleaning tank is fixedly connected to an air access pipe, a second valve body is fixedly installed in the middle of the air access pipe, and steam or sterile air is injected into the cleaning tank through the air access pipe.

[0010] Preferably, the bottom end of the refining and purification tank is fixedly connected to a product discharge pipe, one side of the ceramic membrane system is fixedly connected to a connecting pipe, the side of the connecting pipe away from the ceramic membrane system is fixedly connected to the side opposite the refining and purification tank, and a third valve body is fixedly installed in the middle of the connecting pipe. The product in the refining and purification tank is discharged to the outside through the product discharge pipe, and the product in the ceramic membrane system is transported to the refining and purification tank through the connecting pipe.

[0011] Preferably, the top of one side of the cleaning tank is fixedly connected with an injection pipe, the end of the injection pipe away from the cleaning tank is fixedly connected to the side facing the ceramic membrane system, a second pressure gauge is fixedly installed on the surface of the injection pipe, the middle part of the injection pipe is fixedly connected with an exhaust pipe, the middle part of the exhaust pipe is fixedly installed with a fourth valve body, and one end of the injection pipe is fixedly installed with a fifth valve body. The cleaning liquid in the cleaning tank is injected into the ceramic membrane system through the injection pipe, and the second pressure gauge senses the pressure value of the injection pipe in real time, and transmits the gas in the cleaning liquid to be discharged to the outside through the exhaust pipe.

[0012] Preferably, a transition pipe is fixedly connected between the circulation pump and the feed pump, and a sixth valve body is fixedly installed in the middle of the transition pipe.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] 1. Significantly reduce the number of initial bacterial culture and seed tank culture times;

[0015] 2. The fermentation level has been maintained at a high level, the acid production rate of the bacteria is high, and the output is significantly increased without increasing the equipment. The overall output can even reach 2-3 times the original output;

[0016] 3. The nutrients in the ceramic membrane filtrate can be sterilized after the product is extracted and added to the fermentation tank as a nutrient stream, thereby increasing the product output ratio of the feed, that is, increasing the acid production rate;

[0017] 4. The ceramic membrane can be sterilized with steam, ensuring the sterile equipment requirements;

[0018] 5. Since the utilization rate of bacteria is increased several times, the amount of bacteria to be processed after sterilization is reduced by 60-70%, while a large amount of discharged water is reduced, which saves energy and is environmentally friendly, and reduces the cost of waste and wastewater treatment;

[0019] 6. Since the number of inoculations is reduced, the risk of infection is greatly reduced;

[0020] 7. Since the ceramic membrane equipment is also a device that needs to filter bacteria in single batch fermentation, the ceramic membrane realizes two functions in the entire process, and there is basically no additional equipment investment in the entire project;

[0021] 8. The post-processing of a single fermentation is batch processing. It usually takes about 20 hours to process the entire batch of materials. During these 20 hours, the fermentation liquid bacteria are prone to autolysis and the bacteria consume products, which increases the burden of post-processing. Now with continuous output, the product enters the post-processing immediately after it is produced, realizing instant refining and purification, which not only improves efficiency but also reduces the risk of reverse fermentation of the product. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the present utility model.

[0023] In the figure: 1. Inoculum tank; 2. First inoculation pipeline; 3. Seed tank; 4. Second inoculation pipeline; 5. Fermentation tank; 6. First delivery pipeline; 7. Feed pump; 8. First extraction pipeline; 9. Feed tank; 10. Feed pipeline; 11. Nutrient solution sterilization and reuse pipeline; 12. Cleaning tank; 13. Circulation pump; 14. Filter; 15. Second extraction pipeline; 16. Second delivery pipeline; 17. Air access pipeline; 18. First valve body; 19. Second valve body; 20. First pressure gauge; 21. Ceramic membrane system; 22. Refining and purification tank; 23. Product discharge pipeline; 24. Connecting pipeline; 25. Third valve body; 26. Second pressure gauge; 27. Injection pipeline; 28. Exhaust pipeline; 29. ​​Fourth valve body; 30. Fifth valve body; 31. Transition pipeline; 32. Sixth valve body. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present invention.

[0025] See also Figure 1 The utility model provides an amino acid online circulation flow and fermentation system using a ceramic membrane, comprising a strain tank 1, a seed tank 3 is provided on one side of the strain tank 1, a fermentation tank 5 is provided on one side of the seed tank 3, a feeding pump 7 is provided on the top of one side of the fermentation tank 5, a cleaning tank 12 is provided on one side of the fermentation tank 5, a feeding tank 9 is provided in the middle of one side of the fermentation tank 5, a circulation pump 13 is provided on the bottom end of one side of the cleaning tank 12, a ceramic membrane system 21 is provided on one side of the cleaning tank 12, a refining and purification tank 22 is provided on one side of the ceramic membrane system 21, a first inoculation pipe 2 is fixedly connected to one side of the strain tank 1, an end of the first inoculation pipe 2 away from the strain tank 1 is fixedly connected to the side opposite to the seed tank 3, a second inoculation pipe 4 is fixedly connected to the side of the seed tank 3 away from the strain tank 1, a side of the second inoculation pipe 4 away from the seed tank 3 is fixedly connected to the side opposite to the fermentation tank 5, the bacteria in the strain tank 1 are cultured and transported to the seed tank 3 through the first inoculation pipe 2, and the seeds in the seed tank 3 are transported to the fermentation tank 5 through the second inoculation pipe 4.

[0026] One side of the top of the feeding tank 9 is fixedly connected to a feeding pipe 10, and the other side of the top of the feeding tank 9 is fixedly connected to a nutrient solution sterilization return pipe 11. The nutrient solution sterilization return pipe 11 is fixedly connected to the side opposite the refining and purification tank 22 at one end away from the feeding tank 9. The liquid inlet of the feeding pump 7 is fixedly connected to a first extraction pipe 8 extending to the inside of the feeding tank 9, and the liquid outlet of the feeding pump 7 is fixedly connected to a first conveying pipe 6 extending to the inside of the fermentation tank 5. The feeding pump 7 is started after being powered on, and the feeding pump 7 extracts the product in the feeding tank 9 through the first extraction pipe 8, and the extracted product is conveyed to the fermentation tank 5 through the first conveying pipe 6.

[0027] The liquid outlet of the circulation pump 13 is fixedly connected to a second extraction pipe 15 extending into the interior of the fermentation tank 5, and the liquid inlet of the circulation pump 13 is fixedly connected to a second delivery pipe 16 extending into the interior of the ceramic membrane system 21. A first valve body 18 is fixedly installed in the middle of the second extraction pipe 15, and a first pressure gauge 20 is fixedly installed in the middle of the second delivery pipe 16. A filter 14 is fixedly installed inside the second extraction pipe 15. The circulation pump 13 extracts the product in the ceramic membrane system 21 through the second delivery pipe 16, and the extracted product is delivered to the fermentation tank 5 through the second extraction pipe 15, and the first pressure gauge 20 displays the pressure of the second delivery pipe 16 in real time.

[0028] The bottom end of the cleaning tank 12 is fixedly connected to an air access pipe 17 , and a second valve body 19 is fixedly installed in the middle of the air access pipe 17 . Steam or sterile air is injected into the cleaning tank 12 through the air access pipe 17 .

[0029] The bottom end of the refining and purification tank 22 is fixedly connected to a product discharge pipe 23, and one side of the ceramic membrane system 21 is fixedly connected to a connecting pipe 24. The side of the connecting pipe 24 away from the ceramic membrane system 21 is fixedly connected to the side directly opposite the refining and purification tank 22. A third valve body 25 is fixedly installed in the middle of the connecting pipe 24. The product in the refining and purification tank 22 is discharged to the outside through the product discharge pipe 23, and the product in the ceramic membrane system 21 is transported to the refining and purification tank 22 through the connecting pipe 24.

[0030] The top of one side of the cleaning tank 12 is fixedly connected with an injection pipe 27, and the end of the injection pipe 27 away from the cleaning tank 12 is fixedly connected to the side facing the ceramic membrane system 21. A second pressure gauge 26 is fixedly installed on the surface of the injection pipe 27, and the middle part of the injection pipe 27 is fixedly connected with an exhaust pipe 28. A fourth valve body 29 is fixedly installed in the middle part of the exhaust pipe 28, and a fifth valve body 30 is fixedly installed at one end of the injection pipe 27. The cleaning liquid in the cleaning tank 12 is injected into the ceramic membrane system 21 through the injection pipe 27, and the second pressure gauge 26 senses the pressure value of the injection pipe 27 in real time, and transmits the gas in the cleaning liquid to be discharged to the outside through the exhaust pipe 28.

[0031] A transition pipe 31 is fixedly connected between the circulation pump 13 and the feed pump 7 , and a sixth valve body 32 is fixedly installed in the middle of the transition pipe 31 .

[0032] When the embodiment of the present application is in use: the fermentation tank 5 and the ceramic membrane system 21 are connected. In order to ensure the sterility of the ceramic membrane system 21, the tank 12 is first cleaned with hot water and a high concentration of alkali solution to sterilize the inside of the membrane equipment. After sterilization, the membrane equipment is also constantly passed through sterile air to maintain a positive pressure inside the membrane equipment. This can ensure that the entire equipment pipeline is sterile during the entire production process of the membrane equipment, thereby avoiding contamination of the fermentation tank 5. We connect the ceramic membrane feed pipeline to the fermentation tank 5, and the concentrated liquid pipeline of the ceramic membrane also flows back to the fermentation tank 5. The filtrate pipeline of the membrane is connected to the product tank, also called the refining and purification tank 22. In this way, after the bacteria ferment to the logarithmic growth period, the ceramic membrane system 21 is started to circulate the feed liquid in the fermentation tank 5 in the fermentation tank 5 and the membrane equipment. The ceramic membrane brings the product out through the filtrate during the filtration process, and macromolecular substances such as bacteria are concentrated and returned to the fermentation tank 5. In addition, a feeding tank 9 for continuously adding culture medium is set next to the fermentation tank 5 to ensure that the volume and composition of the culture medium in the fermentation tank 5 remain basically unchanged. Since the ceramic membrane filtration brings the product out of the fermentation tank 5, as the product is continuously discharged from the fermentation tank 5, the concentration of the product in the fermentation tank 5 is always at a low level, reducing the inhibition of the product. At the same time, the concentration of the bacteria has basically no change. Since the bacteria are not inhibited by the product, the bacteria are very active. The entire fermentation process is in the logarithmic phase of the bacteria. At this time, the fermentation level is also the highest, the acid production is at the highest level, and the production efficiency is very high. In this way, continuous flow feeding and long-term fermentation are carried out continuously. Generally, such a fermentation can last for 5-6 times the fermentation time of a single batch fermentation, and during this time, it is at the stage with the highest acid production level, so the output per unit time is very high, and a lot of unit cost and time are saved.

[0033] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A system for realizing online circulation and fermentation of amino acids using ceramic membranes, comprising a bacterial seed tank (1), characterized in that: A seed tank (3) is provided on one side of the bacterial seed tank (1), a fermentation tank (5) is provided on one side of the seed tank (3), a feeding pump (7) is provided on the top of one side of the fermentation tank (5), a cleaning tank (12) is provided on one side of the fermentation tank (5), a feeding tank (9) is provided in the middle of one side of the fermentation tank (5), a circulation pump (13) is provided on the bottom end of one side of the cleaning tank (12), a ceramic membrane system (21) is provided on one side of the cleaning tank (12), a refining and purification tank (22) is provided on one side of the ceramic membrane system (21), a first inoculation pipe (2) is fixedly connected to one side of the bacterial seed tank (1), an end of the first inoculation pipe (2) away from the bacterial seed tank (1) is fixedly connected to a side directly opposite to the seed tank (3), a second inoculation pipe (4) is fixedly connected to one side of the seed tank (3) away from the bacterial seed tank (1), and a side of the second inoculation pipe (4) away from the seed tank (3) is fixedly connected to a side directly opposite to the fermentation tank (5).

2. The amino acid online circulation and fermentation system using a ceramic membrane according to claim 1, characterized in that: One side of the top of the feeding tank (9) is fixedly connected to a feeding pipe (10), and the other side of the top of the feeding tank (9) is fixedly connected to a nutrient solution sterilization return pipe (11). The end of the nutrient solution sterilization return pipe (11) away from the feeding tank (9) is fixedly connected to the side directly opposite the refining and purification tank (22). The liquid inlet of the feeding pump (7) is fixedly connected to a first extraction pipe (8) extending into the interior of the feeding tank (9), and the liquid outlet of the feeding pump (7) is fixedly connected to a first delivery pipe (6) extending into the interior of the fermentation tank (5).

3. The amino acid online circulation and fermentation system using a ceramic membrane according to claim 1, characterized in that: The liquid outlet of the circulation pump (13) is fixedly connected to a second extraction pipe (15) extending to the interior of the fermentation tank (5), and the liquid inlet of the circulation pump (13) is fixedly connected to a second delivery pipe (16) extending to the interior of the ceramic membrane system (21). A first valve body (18) is fixedly installed in the middle of the second extraction pipe (15), a first pressure gauge (20) is fixedly installed in the middle of the second delivery pipe (16), and a filter (14) is fixedly installed inside the second extraction pipe (15).

4. The amino acid online circulation and fermentation system using a ceramic membrane according to claim 1, characterized in that: The bottom end of the cleaning tank (12) is fixedly connected to an air access pipe (17), and a second valve body (19) is fixedly installed in the middle of the air access pipe (17).

5. The amino acid online circulation and fermentation system using a ceramic membrane according to claim 1, characterized in that: The bottom end of the refining and purification tank (22) is fixedly connected to a product discharge pipe (23), and one side of the ceramic membrane system (21) is fixedly connected to a connecting pipe (24). The side of the connecting pipe (24) away from the ceramic membrane system (21) is fixedly connected to the side directly opposite the refining and purification tank (22), and a third valve body (25) is fixedly installed in the middle of the connecting pipe (24).

6. The amino acid online circulation and fermentation system using a ceramic membrane according to claim 1, characterized in that: The top of one side of the cleaning tank (12) is fixedly connected to an injection pipe (27), the end of the injection pipe (27) away from the cleaning tank (12) is fixedly connected to the side facing the ceramic membrane system (21), a second pressure gauge (26) is fixedly installed on the surface of the injection pipe (27), the middle of the injection pipe (27) is fixedly connected to an exhaust pipe (28), the middle of the exhaust pipe (28) is fixedly installed with a fourth valve body (29), and one end of the injection pipe (27) is fixedly installed with a fifth valve body (30).

7. The amino acid online circulation and fermentation system using a ceramic membrane according to claim 1, characterized in that: A transition pipe (31) is fixedly connected between the circulation pump (13) and the feed pump (7), and a sixth valve body (32) is fixedly installed in the middle of the transition pipe (31).

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