Constant-temperature heating device suitable for cell culture

Abstract

According to the constant-temperature heating device suitable for cell culture, high-temperature air generated by the heating air box is led into the rear part of the cell culture box through the heat insulation air pipe and the turbulent flow pipe, and the design of the turbulent flow pipe can disturb an air flowing path, so that hot air is uniformly diffused in the cell culture box, direct blowing of the high-temperature air is avoided, and the service life of the cell culture box is prolonged. The structure of the cell culture box is effectively protected, the service life of the cell culture box is prolonged, uneven temperature distribution in the box is avoided, and the cell culture environment is more stable. Steam is generated through the steam heating device and output into the culture box, the humidity is controlled while the temperature is controlled, it is guaranteed that the cells are always in the proper temperature and humidity condition, and the cell culture efficiency is further improved. Compared with a traditional method that a heating fan is installed on the longitudinal side wall of the cell culture box, an experiment operator can easily stretch the hand into the cell culture box to conduct an experiment, the size is reduced, operation is convenient, and meanwhile the risk that the experiment operator is scalded by high temperature is avoided.

Description

Technical Field

[0001] The utility model belongs to the field of cell culture, and in particular relates to a constant temperature heating device suitable for cell culture. Background Art

[0002] Cell culture refers to a technology that simulates the in vivo environment in vitro to enable cells or biological tissues to grow, reproduce, and maintain their main structures and functions. In scientific research work that focuses on in vitro cultured cells, it is usually necessary to combine cell culture technology with microscopic imaging technology to study the physiological indicators of cells under normal cell culture environments. Currently, there are commercial cell culture systems that can be used with microscopes.

[0003] Existing microscope-mounted cell culture devices have the following major drawbacks: First, to maintain a normal cell culture environment, air at a certain temperature must be introduced into the microscope-mounted cell culture system. However, when high-temperature air is blown into the cell culture chamber, it can easily lead to localized high temperatures within the chamber. This localized high temperature can not only affect the cell state but also increase the temperature of the cell culture chamber itself, significantly reducing the device's service life and affecting experimental results. Second, existing microscope-mounted cell culture devices occupy a large footprint, making them inconvenient to operate. Utility Model Content

[0004] The purpose of the present invention is to provide a constant temperature heating device suitable for cell culture, so as to solve the problems in the prior art of cell culture devices such as uneven internal temperature and large floor space, which may cause adverse effects on cells, shorten the life of the equipment and be unfavorable for operation.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0006] A constant temperature heating device suitable for cell culture, comprising a cell culture chamber, a heating bellows, and a humidity control assembly; the cell culture chamber can carry a microscope, a culture chamber is provided on the stage of the microscope, and a heating fan is provided inside the heating bellows; the humidity control assembly is in communication with the interior of the culture chamber; the device is characterized in that air outlet pipes are connected to both sides of the heating bellows, and through holes are formed on both sides of the rear of the cell culture chamber; a spoiler pipe is fixedly connected to the interior of each through hole, the spoiler pipe comprising an air inlet pipe and two air outlet pipes, and the air outlet pipes of the spoiler pipes are located inside the cell culture chamber;

[0007] The two air outlet pipes of the heating bellows are respectively connected to one end of an insulated air duct; the other end of the insulated air duct is connected to the air inlet pipe of the corresponding spoiler pipe;

[0008] The central axes of the two air outlet ducts of the spoiler pipe are located in the same plane inclined downward by 45 degrees, and the angle between them is 75 degrees.

[0009] The utility model also has the following features:

[0010] Furthermore, the inner diameter of the heat-insulated air duct is 100 mm;

[0011] The distance between the heating fan inside the heating bellows and the inner walls on both sides of the heating bellows is 145 mm;

[0012] The distance between the rear wall of the heating fan and the front wall of the heating air box is 125 mm.

[0013] Furthermore, the inner diameter of the air inlet duct of the spoiler tube is 100 mm, and the inner diameter of the air outlet duct is 85 mm.

[0014] Furthermore, the heat-insulating air duct is a flexible tube.

[0015] Further, the humidity control assembly includes a humidity control box;

[0016] The humidity control box is provided with an air guide hole, which can be connected to the output end of an external air guide device; the humidity control box is internally provided with an air guide pipe, one end of the air guide pipe is connected to the air inlet, and the other end is connected to the input end of the gas flow meter;

[0017] The output end of the gas flow meter is connected to the input end of the steam heater through a pipeline, the output end of the steam heater is connected to the mixed steam output device, and the output end of the mixed steam output device is connected to the culture box on the stage of the microscope;

[0018] A temperature sensor and a humidity sensor are arranged inside the culture box.

[0019] Furthermore, a controller is included;

[0020] The controller is connected to the gas flow meter of the humidity control box, the steam heater, the heating fan of the heating bellows 2, the temperature sensor and the humidity sensor respectively.

[0021] Furthermore, the inner wall of the cell culture chamber is provided with reinforcing plates having the same number as the spoiler tubes;

[0022] A through hole having the same diameter as the through hole of the cell culture box 1 is opened in the middle of the reinforcing plate, and the installation position of each reinforcing plate corresponds to the position of the through hole of the cell culture box 1 one by one.

[0023] Furthermore, mounting holes are provided on opposite side walls of the air outlet duct of the heating bellows;

[0024] The two side walls of the heat-insulating air duct are provided with threaded holes corresponding to the mounting holes of the air outlet pipes;

[0025] The outer diameter of the heat-insulating air duct matches the inner diameter of the air outlet pipe, and the air outlet pipe and the heat-insulating air duct can be detachably connected by screwing.

[0026] Compared with the prior art, the present invention has the following technical effects:

[0027] (I) The constant temperature heating device for cell culture of the present invention uses a heating bellows to generate high temperature air which is introduced into the rear of the cell culture chamber through a heat-insulating air duct and a spoiler tube. The spoiler tube is designed to disrupt the air flow path, thereby evenly diffusing the hot air in the cell culture chamber and avoiding direct blowing of high temperature air. This effectively protects the structure of the cell culture chamber and extends its service life. At the same time, it avoids uneven temperature distribution in the chamber, making the cell culture environment more stable.

[0028] At the same time, the humidity control component of the present invention generates steam through a steam heating device and outputs it to the culture box, controlling the temperature and humidity at the same time, ensuring that the cells are always under appropriate temperature and humidity conditions, further improving the efficiency of cell culture.

[0029] In addition, the combination of a heating bellows and a spoiler tube can effectively reduce the occupied area and increase the experimental operation space in the cell culture incubator. Compared with the traditional method of installing the heating fan on the longitudinal side wall of the cell culture incubator, the experimental operator can not only easily put his hand into the cell culture incubator to conduct experiments, but also reduce the volume for easy operation while avoiding the risk of high temperature burns for the experimenter.

[0030] (II) After the constant temperature heating device for cell culture of the present invention adopts a heating bellows to supply heat, the air temperature at the air inlet of the spoiler tube is about 60-70 degrees Celsius, and the temperature of the contact surface between the outer wall of the spoiler tube and the cell culture box is about 40-50 degrees Celsius. At this temperature, the service life of the cell culture box will be greatly extended.

[0031] (III) The constant temperature heating device for cell culture of the present invention is further designed in terms of component size and installation position according to actual needs. The size and installation position of the flow-turbulating tube fully utilize the free space in the cell culture chamber, without causing any impact on the operator's control of the instrument and equipment. This design not only increases the available area for the operator but also reduces the volume occupied by the equipment, effectively saving space while improving the sealing performance, making it suitable for large-scale use and promotion in industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a schematic diagram of the overall structure of the constant temperature heating device suitable for cell culture of the utility model;

[0033] Figure 2 This is a schematic diagram of the heating bellows structure in the utility model;

[0034] Figure 3 This is a schematic diagram of the structure of the flow-disturbing tube in the present invention installed in a cell culture box;

[0035] Figure 4 This is a schematic diagram of the installation structure of the flow-disturbing tube and the cell culture box in the present invention at another angle;

[0036] Figure 5 It is a schematic diagram of the simulation analysis effect in one embodiment of the present utility model.

[0037] The meaning of the symbols in the figure is:

[0038] 1. Cell culture chamber; 2. Heating bellows; 3. Heating fan; 4. Air outlet duct; 5. Turbine; 6. Insulated air duct; 7. Humidity control box; 8. Reinforcement plate; 9. Mounting hole; 501. Air inlet duct; 502. Air outlet duct. DETAILED DESCRIPTION

[0039] It should be noted that, unless otherwise specified, all components in the present invention are components known in the prior art. For example, the steam heater is a known and commonly used steam heater.

[0040] The following are specific embodiments of the present invention. It should be noted that the present invention is not limited to the following specific embodiments, and all equivalent modifications made on the basis of the technical solution of this application fall within the scope of protection of the present invention.

[0041] like Figure 1-4 As shown, a constant temperature heating device suitable for cell culture comprises a cell culture box 1, a heating bellows 2 and a humidity control component; the cell culture box 1 can carry a microscope, a culture box is provided on the stage of the microscope, and a heating fan 3 is provided inside the heating bellows 2; the humidity control component is connected to the interior of the culture box; it is characterized in that air outlet pipes 4 are connected to both sides of the heating bellows 2, a spoiler pipe 5 is fixedly connected to the inside of each through hole, the spoiler pipe 5 comprises an air inlet pipe 501 and two air outlet pipes 502, and the air outlet pipes 502 of the spoiler pipe 5 are located inside the cell culture box 1; the two air outlet pipes 4 of the heating bellows 2 are respectively connected to one end of an insulated air pipe 6; the other end of the insulated air pipe 6 is connected to the air inlet pipe 501 of the corresponding spoiler pipe 5;

[0042] The central axes of the two air outlet ducts 502 of the spoiler 5 are located in the same plane inclined downward by 45 degrees and the angle between them is 75 degrees. This design of the air outlet duct 502 can effectively help the high-temperature air to diffuse evenly from bottom to top and from left to right in the cell culture chamber 1;

[0043] In one embodiment of the present invention, the heating bellows 1 is made of nylon plastic material, so that the heating bellows 1 has the characteristics of high temperature resistance and acid and alkali corrosion resistance;

[0044] The constant temperature heating device suitable for cell culture of the present invention adopts a heating bellows to generate high-temperature air, which is introduced into the rear of the cell culture box 1 through the heat-insulating air duct 6 and the spoiler tube 5. The design of the spoiler tube 5 can disrupt the air flow path, so that the hot air can be evenly diffused in the cell culture box 1, avoiding direct blowing of high-temperature air, effectively protecting the cell culture box 1, extending its service life, and avoiding uneven temperature distribution in the box, making the cell culture environment more stable.

[0045] At the same time, the humidity control component of the present invention generates steam through a steam heating device and outputs it to the culture box, controlling the temperature and humidity at the same time, ensuring that the cells are always under appropriate temperature and humidity conditions, further improving the efficiency of cell culture.

[0046] In addition, the combination of the heating bellows 2 and the spoiler tube 5 can effectively reduce the occupied area and increase the experimental operation space in the cell culture box 1. Compared with the traditional method of installing the heating fan 3 on the longitudinal side wall of the cell culture box 1, the experimental operator can not only easily put his hand into the cell culture box to conduct experiments, but also avoid the risk of high temperature burns for the experimenter while reducing the volume for easy operation.

[0047] A specific numerical value selection is given below. The inner diameter of the heat-insulating air duct 6 is 100 mm;

[0048] The distance between the heating fan 3 inside the heating air box 2 and the inner walls on both sides of the heating air box 2 is 145 mm;

[0049] The distance between the rear wall of the heating fan 3 and the front wall of the heating air box is 125 mm.

[0050] The inner diameter of the air inlet duct 501 of the spoiler tube 5 is 100 mm, and the inner diameter of the air outlet duct 502 is 85 mm.

[0051] As a preferred solution, the heat-insulating air duct 6 is a flexible tube. Optionally, the heat-insulating air duct 6 is made of nylon cloth, which is a flexible material, so that the heat-insulating air duct 6 has the characteristics of high temperature resistance, acid and alkali corrosion resistance and certain stretching, expansion and contraction and bending functions.

[0052] As a preferred solution, the humidity control assembly includes a humidity control box 7;

[0053] The humidity control box 7 is provided with an air guide hole, which can be connected to the output end of the external air guide device; the humidity control box 7 is provided with an air guide pipe inside, one end of the air guide pipe is connected to the air inlet, and the other end is connected to the input end of the gas flow meter;

[0054] The output end of the gas flow meter is connected to the pipeline of the input end of the steam heater, the output end of the steam heater is connected to the mixed steam output device, and the output end of the mixed steam output device is connected to the culture box on the stage of the microscope;

[0055] A temperature sensor and a humidity sensor are provided inside the incubator.

[0056] Among them, the gas flow meter, steam heater, mixed steam output device, temperature sensor and humidity sensor are all commonly used devices known in the art.

[0057] In this embodiment, during conventional cell culture, filtered air and 99.999% CO2 are introduced into the air guide holes of the humidity control box 7, and the mixing ratio of the two gases is controlled by a gas flow meter to fully meet the culture environment requirements of different cells. The mixed gas is transported to the steam heater, and steam is generated by the water in the steam heater. Steam is then transported to the culture box in the cell culture incubator 1 through a mixed steam output device with the mixed gas.

[0058] Further preferably, it further includes a controller;

[0059] The controller is connected to the gas flow meter of the humidity control box 7, the steam heater, the heating fan 3 of the heating wind box 2, the temperature sensor and the humidity sensor respectively.

[0060] It should be noted that the controller adopts a known existing controller and controls the remaining components through conventional algorithms to achieve automation of the device.

[0061] As a preferred solution, the inner wall of the cell culture chamber 1 is provided with reinforcing plates 8 whose number is the same as the number of the spoiler tubes 5;

[0062] A through hole with the same diameter as the through hole of the cell culture box 1 is opened in the middle of the reinforcing plate 8, and the installation position of each reinforcing plate 8 corresponds one-to-one to the position of the through hole of the cell culture box 1. This setting can enhance the installation strength of the spoiler tube 5 and the cell culture box 1.

[0063] As a preferred solution, mounting holes 9 are provided on the opposite side walls of the air outlet duct 4 of the heating air box 2;

[0064] The two side walls of the heat-insulating air duct 6 are provided with threaded holes corresponding to the mounting holes of the air outlet pipe 4;

[0065] The outer diameter of the heat-insulating air duct 6 matches the inner diameter of the air outlet pipe 4, and the air outlet pipe 4 and the heat-insulating air duct 6 can be detachably connected by screwing. This arrangement facilitates the assembly and disassembly of the heat-insulating air duct 6 and enhances the convenience of the overall device.

[0066] like Figure 5 As shown in the figure, a COMSOL simulation of the thermal diffusion flow field simulates the temperature change of hot air entering a space of the same size and volume as the cell culture chamber 1 through the spoiler tube 5, assuming the air inlet velocity and temperature are consistent with the actual wind speed and temperature provided by the actual heating bellows 2. In this simulation, parameters such as the installation position and dimensions of the spoiler tube 5 are consistent with the actual conditions. The simulation results show that when the cell culture chamber 1 is fully enclosed and the heating bellows 2 is running at full power, the air in the cell culture chamber 1 can be heated from 24 degrees Celsius to 37 degrees Celsius in 15 minutes and maintain a relatively stable temperature environment. In reality, in order to be installed on an inverted biological microscope, the cell culture chamber 1 has a certain opening, which prolongs the time it takes to reach and maintain a stable temperature at 37 degrees Celsius. Through simulation, the temperature in the cell culture chamber can reach and maintain a relatively stable temperature at 37 degrees Celsius in 30 minutes. At the same time, multiple actual tests were conducted on the prototype, and the average time it took to maintain a stable temperature was 33.8 minutes, which is close to the simulation results.

Claims

1. A constant temperature heating device suitable for cell culture, comprising a cell culture box (1), a heating bellows (2) and a humidity control component; the cell culture box (1) can be equipped with a microscope, a culture box is provided on the stage of the microscope, a heating fan (3) is provided inside the heating bellows (2); the humidity control component is connected to the interior of the culture box; and the invention is characterized in that: The heating air box (2) is connected to air outlet pipes (4) on both sides, and the cell culture box (1) is provided with through holes on both sides of the rear portion; a spoiler pipe (5) is fixedly connected to the inside of each through hole, and the spoiler pipe (5) includes an air inlet pipe (501) and two air outlet pipes (502), and the air outlet pipe (502) of the spoiler pipe (5) is located inside the cell culture box (1); The two air outlet pipes (4) of the heating bellows (2) are respectively connected to one end of a heat-insulating air duct (6); the other end of the heat-insulating air duct (6) is connected to the air inlet pipe (501) of the corresponding spoiler pipe (5); The central axes of the two air outlet ducts (502) of the spoiler tube (5) are located in the same plane inclined downward by 45 degrees, and the angle between them is 75 degrees.

2. The constant temperature heating device suitable for cell culture according to claim 1, characterized in that: The inner diameter of the heat-insulating air duct (6) is 100 mm; The distance between the heating fan (3) inside the heating bellows (2) and the inner walls on both sides of the heating bellows (2) is 145 mm; The distance between the rear wall of the heating fan (3) and the front wall of the heating air box is 125 mm.

3. The constant temperature heating device suitable for cell culture according to claim 2, characterized in that: The inner diameter of the air inlet duct (501) of the spoiler tube (5) is 100 mm, and the inner diameter of the air outlet duct (502) is 85 mm.

4. The constant temperature heating device suitable for cell culture according to claim 1, characterized in that: The heat-insulating air duct (6) is a flexible tube.

5. The constant temperature heating device suitable for cell culture according to claim 1, characterized in that: The humidity control assembly includes a humidity control box (7); The humidity control box (7) is provided with an air guide hole, which can be connected to the output end of an external air guide device; the humidity control box (7) is internally provided with an air guide pipe, one end of the air guide pipe is connected to the air inlet, and the other end is connected to the input end of the gas flow meter; The output end of the gas flow meter is connected to the input end of the steam heater through a pipeline, the output end of the steam heater is connected to the mixed steam output device, and the output end of the mixed steam output device is connected to the culture box on the stage of the microscope; A temperature sensor and a humidity sensor are arranged inside the culture box.

6. The constant temperature heating device suitable for cell culture according to claim 5, characterized in that: Also includes a controller; The controller is connected to the gas flow meter of the humidity control box (7), the steam heater, the heating fan (3) of the heating wind box (2), the temperature sensor and the humidity sensor respectively.

7. The constant temperature heating device suitable for cell culture according to claim 1, characterized in that: The inner wall of the cell culture box (1) is provided with reinforcing plates (8) whose number is the same as the number of the spoiler tubes (5); A through hole having the same diameter as the through hole of the cell culture box (1) is provided in the middle of the reinforcing plate (8), and the installation position of each reinforcing plate (8) corresponds one-to-one to the position of the through hole of the cell culture box (1).

8. The constant temperature heating device suitable for cell culture according to claim 1, characterized in that: Mounting holes (9) are provided on opposite side walls of the air outlet pipe (4) of the heating bellows (2); The two side walls of the heat-insulating air duct (6) are provided with threaded holes corresponding to the mounting holes of the air outlet duct (4); The outer diameter of the heat-insulating air duct (6) matches the inner diameter of the air outlet pipe (4), and the air outlet pipe (4) and the heat-insulating air duct (6) can be detachably connected by screwing.

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