Negative pressure collection device for suspension cells cultured by culture bag

Abstract

The utility model discloses a culture bag culture suspension cell negative pressure collection device, which comprises a device table body, a turntable, centrifugal bottles, a culture bag body and a sealing plate, the turntable is rotatably arranged above the device table body, the top end of the turntable is provided with positioning grooves at equal intervals, the centrifugal bottles are arranged inside the positioning grooves, and the sealing plate is arranged on the turntable. A bottle opening is formed in the top end of the centrifugal bottle, a liquid inlet pipe and an exhaust pipe are installed in the bottle opening, the sealing plate is arranged at the top end of the bottle opening, a hanging frame is fixed to the upper portion of the rotating disc, hanging rods are fixed to the outer wall of the top of the hanging frame at equal intervals, and the culture bag body is hung on the hanging rods. According to the utility model, suspension cell culture fluid in a plurality of culture bag bodies can be collected at a time, the continuity of vacuumizing and fluid collection operation is high, the operation is convenient, the efficiency is high, and the centrifugal bottle adopts a sealed structure and is not easy to pollute.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a negative pressure collection device for cultured suspended cells in a culture bag. Background Art

[0002] Suspension cells are cells that grow independently of a support surface and instead remain suspended in a culture medium. Culture bags are made from special breathable materials and are capable of supporting high-density cell culture. They are suitable for culturing suspension cells, boasting a large gas exchange area, allowing for greater cell numbers compared to culture flasks, and offer a strong containment structure. They are widely used in the current biomedical field, particularly in cell therapy.

[0003] After the suspension cells are cultured in the culture bag, they need to be centrifuged before they can be collected. Therefore, the culture fluid in the culture bag needs to be collected into a centrifuge bottle. The commonly used collection method is negative pressure collection. However, when collecting the suspension cell culture fluid, the staff needs to first vacuum the centrifuge bottle, then hold the culture bag and the centrifuge bottle and connect them with a catheter. The operation is very cumbersome and can only be collected one by one, which is inefficient. In addition, the opening of the centrifuge bottle is exposed to the outside and is easily contaminated, so it is urgently needed to be improved. Utility Model Content

[0004] The purpose of the utility model is to provide a negative pressure collection device for cultured suspended cells in a culture bag, so as to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a negative pressure collection device for culturing suspended cells in a culture bag, comprising a device platform, a turntable, a centrifuge bottle, a culture bag body and a sealing plate, the turntable being rotatably mounted above the device platform, and the top of the turntable being provided with positioning grooves at equal intervals, the centrifuge bottle being arranged inside the positioning groove, and the top of the centrifuge bottle being provided with a bottle mouth, and a liquid inlet pipe and an air extraction pipe being respectively installed inside the bottle mouth, the sealing plate being arranged at the top of the bottle mouth, and the sealing plate being slidably connected to the damping slide groove at the top of the bottle mouth, a hanging rack being fixed above the turntable, and hanging rods at equal intervals being fixed on the outer wall of the top of the hanging rack, the culture bag body being suspended on the hanging rods, and a sealed liquid outlet being installed at the bottom end of the culture bag body, the bottom end of the sealed liquid outlet being connected to a liquid collecting pipe, and a vacuum pump being installed at the center position of the top of the turntable.

[0006] Preferably, walking wheels are installed at the corner positions of the bottom end of the device platform to facilitate the movement of the device.

[0007] Preferably, an annular groove is provided at the edge of the top of the device platform, and rollers are installed at the bottom of the turntable at the position of the annular groove. The rollers and the annular groove cooperate with each other to make the turntable rotate smoothly.

[0008] Preferably, an annular tube is installed above the vacuum pump, and the annular tube is connected to the input end of the vacuum pump.

[0009] Preferably, the outer wall of the annular tube is connected to vacuum tubes at equal intervals, so as to facilitate vacuuming of multiple centrifuge bottles at the same time.

[0010] Preferably, a second flow-stopping clamp is mounted on the surface of the vacuum tube to control the connectivity of the vacuum tube.

[0011] Preferably, the surface of the liquid collecting pipe is provided with a first flow-stopping clamp, which can control the connectivity state of the liquid collecting pipe.

[0012] Preferably, the liquid collecting pipe and the liquid inlet pipe cooperate with each other, and the vacuum tube and the air extraction pipe cooperate with each other.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] Place the centrifuge bottles in the positioning slots at the top of the turntable in sequence, hang the culture bag bodies on the hanging rods in sequence, push the sealing plate to the left to expose the exhaust pipe, connect one end of the vacuum tube to the exhaust pipe, open the second stop clamp, start the vacuum pump to evacuate the inside of each centrifuge bottle through the annular tube and the vacuum tube, then close the second stop clamp, separate the vacuum tube and the exhaust pipe, and push the sealing plate in the opposite direction to bend and seal the exhaust pipe, so that the centrifuge bottles can be quickly evacuated, which is convenient and quick to operate; plug the top of the liquid collecting tube into the sealed liquid outlet, push the sealing plate to the right to expose the liquid inlet pipe, and connect the bottom end of the liquid collecting tube to the inlet The liquid pipe is connected, and then the first stop clamp is opened. Under the negative pressure of the centrifuge bottle, the suspended cell culture fluid inside the culture bag body flows into the centrifuge bottle through the liquid collecting pipe, and the centrifuge bottle collects the culture fluid under negative pressure. After the collection is completed, the liquid collecting pipe and the liquid inlet pipe are separated, and then the sealing plate is pushed in the reverse direction to bend and seal the liquid inlet pipe, which plays an anti-pollution role. The device can collect the suspended cell culture fluid in multiple culture bag bodies at a time, with high efficiency. The centrifuge bottle adopts a sealed structure and is not easily contaminated. Moreover, both the centrifuge bottle and the culture bag body can be placed in the device, freeing both hands. The vacuuming and liquid collecting operations are highly consistent and efficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the main structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the enlarged cross-sectional structure of the centrifuge bottle of the present invention;

[0017] Figure 3 This is a schematic diagram of the enlarged structure of the centrifuge bottle of the present invention from a top view;

[0018] Figure 4 For the utility model Figure 1 A in the middle is an enlarged structural diagram;

[0019] Figure 5 This is a schematic diagram of the enlarged structure of the annular tube in a top view of the present invention.

[0020] In the figure: 1. device platform; 2. turntable; 3. positioning groove; 4. centrifuge bottle; 5. vacuum pump; 6. annular tube; 7. hanging rack; 8. hanging rod; 9. culture bag body; 10. sealed liquid outlet; 11. annular groove; 12. roller; 13. running wheel; 14. bottle mouth; 15. liquid inlet pipe; 16. exhaust pipe; 17. damping slide; 18. sealing plate; 19. liquid collecting pipe; 20. first stop clamp; 21. vacuum tube; 22. second stop clamp. DETAILED DESCRIPTION

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0022] See also Figure 1-5 The utility model provides an embodiment: a negative pressure collection device for cultured suspended cells in a culture bag, comprising a device body 1, a turntable 2, a centrifuge bottle 4, a culture bag body 9 and a sealing plate 18, wherein the turntable 2 is rotatably mounted above the device body 1, and the top of the turntable 2 is provided with positioning grooves 3 at equal intervals, the centrifuge bottle 4 is arranged inside the positioning grooves 3, and the top of the centrifuge bottle 4 is provided with a bottle mouth 14, and the inside of the bottle mouth 14 is respectively provided with a liquid inlet pipe 15 and an air extraction pipe 16;

[0023] The sealing plate 18 is disposed at the top of the bottle mouth 14 and is slidably connected to the damping chute 17 at the top of the bottle mouth 14;

[0024] A hanging frame 7 is fixed above the turntable 2, and evenly spaced hanging rods 8 are fixed to the outer wall of the top of the hanging frame 7. The culture bag body 9 is suspended on the hanging rods 8, and a sealed liquid outlet 10 is installed at the bottom end of the culture bag body 9. The bottom end of the sealed liquid outlet 10 is connected to a liquid collection pipe 19.

[0025] Specifically, the top of the collecting tube 19 is plugged into the sealed liquid outlet 10, the sealing plate 18 is pushed rightward to expose the liquid inlet tube 15, the bottom end of the collecting tube 19 is connected to the liquid inlet tube 15, and the first stop clamp 20 is opened. Under the negative pressure of the centrifuge bottle 4, the suspended cell culture fluid inside the culture bag body 9 flows into the centrifuge bottle 4 through the collecting tube 19. The centrifuge bottle 4 collects the culture fluid under negative pressure. After the collection is completed, the collecting tube 19 and the liquid inlet tube 15 are separated, and the sealing plate 18 is pushed in the opposite direction to bend and seal the liquid inlet tube 15, thereby preventing contamination.

[0026] A vacuum pump 5 is installed at the center of the top of the turntable 2;

[0027] The corners of the bottom of the device body 1 are all equipped with running wheels 13;

[0028] An annular groove 11 is provided at the edge of the top of the device body 1, and a roller 12 is installed at the bottom of the turntable 2 at the position of the annular groove 11, and the roller 12 cooperates with the annular groove 11;

[0029] An annular pipe 6 is installed above the vacuum pump 5, and the annular pipe 6 is connected to the input end of the vacuum pump 5;

[0030] The outer wall of the annular tube 6 is connected to vacuum tubes 21 at equal intervals;

[0031] Specifically, the turntable 2 is rotated so that it rotates synchronously with the hanging rack 7, and the roller 12 rolls along the annular groove 11, so that the turntable 2 and the hanging rack 7 rotate smoothly, and the centrifuge bottles 4 are placed in the positioning grooves 3 at the top of the turntable 2 in sequence, and the culture bag bodies 9 are hung on the hanging rods 8 in sequence;

[0032] Push the sealing plate 18 to the left to expose the exhaust pipe 16, connect one end of the vacuum tube 21 to the exhaust pipe 16, open the second stop clamp 22, and then start the vacuum pump 5 to evacuate the interior of each centrifuge bottle 4 through the annular tube 6 and the vacuum tube 21. Then close the second stop clamp 22, separate the vacuum tube 21 from the exhaust pipe 16, and push the sealing plate 18 in the opposite direction to bend and seal the exhaust pipe 16. This allows the centrifuge bottles 4 to be evacuated quickly, and the operation is convenient and quick.

[0033] A second flow-stopping clamp 22 is mounted on the surface of the vacuum tube 21;

[0034] The surface of the collecting pipe 19 is provided with a first flow-stopping clamp 20;

[0035] The liquid collecting pipe 19 cooperates with the liquid inlet pipe 15 , and the vacuum pipe 21 cooperates with the air extraction pipe 16 .

[0036] When the embodiment of the present application is in use: first, rotate the turntable 2 to make it rotate synchronously with the hanging rack 7, and the roller 12 rolls along the annular groove 11, so that the turntable 2 and the hanging rack 7 rotate smoothly, and the centrifuge bottles 4 are placed in the positioning groove 3 at the top of the turntable 2 in turn, and the culture bag bodies 9 are hung on the hanging rods 8 in turn. Secondly, push the sealing plate 18 to the left to expose the exhaust pipe 16, connect one end of the vacuum tube 21 to the exhaust pipe 16, open the second stop clamp 22, and then start the vacuum pump 5 to evacuate the inside of each centrifuge bottle 4 through the annular tube 6 and the vacuum tube 21, and then close the second stop clamp 22, separate the vacuum tube 21 and the exhaust pipe 16, and push the sealing plate 18 in the opposite direction to bend and seal the exhaust pipe 16, so that the centrifuge bottle 4 can be quickly evacuated, and the operation is convenient and quick. The top end is plugged into the sealed liquid outlet 10, and the sealing plate 18 is pushed to the right to expose the liquid inlet pipe 15, and the bottom end of the collecting pipe 19 is connected to the liquid inlet pipe 15, and then the first stop clamp 20 is opened. Under the negative pressure of the centrifuge bottle 4, the suspended cell culture fluid inside the culture bag body 9 flows into the centrifuge bottle 4 through the collecting pipe 19, and the centrifuge bottle 4 collects the culture fluid under negative pressure. After the collection is completed, the collecting pipe 19 and the liquid inlet pipe 15 are separated, and the sealing plate 18 is pushed in the opposite direction to bend and seal the liquid inlet pipe 15, which plays an anti-pollution role. The device can collect the suspended cell culture fluid in multiple culture bag bodies 9 at a time, with high efficiency. The centrifuge bottle 4 adopts a sealed structure and is not easily contaminated. Moreover, both the centrifuge bottle 4 and the culture bag body 9 can be placed in the device, freeing both hands. The vacuuming and liquid collection operations are highly consistent and efficient.

[0037] Obviously, the embodiments described above are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

Claims

1. A negative pressure collection device for culture bag suspended cells, characterized in that: The device comprises a platform (1), a turntable (2), a centrifuge bottle (4), a culture bag body (9) and a sealing plate (18), wherein the turntable (2) is rotatably mounted above the platform (1), and the top of the turntable (2) is provided with positioning grooves (3) at equal intervals, the centrifuge bottle (4) is arranged inside the positioning grooves (3), and the top of the centrifuge bottle (4) is provided with a bottle mouth (14), and the inside of the bottle mouth (14) is respectively provided with a liquid inlet pipe (15) and an air extraction pipe (16), and the sealing plate (18) is arranged at the bottle mouth (14). The top of the bottle mouth (14) is fixed to the top of the rotary disc (2), and the sealing plate (18) is slidably connected to the damping slide groove (17) at the top of the bottle mouth (14). A hanging frame (7) is fixed above the rotary disc (2), and the outer wall of the top of the hanging frame (7) is fixed with hanging rods (8) at equal intervals. The culture bag body (9) is suspended on the hanging rods (8), and the bottom end of the culture bag body (9) is equipped with a sealed liquid outlet (10), and the bottom end of the sealed liquid outlet (10) is connected to a liquid collecting pipe (19). A vacuum pump (5) is installed at the center position of the top of the rotary disc (2).

2. The negative pressure collection device for cultured suspended cells in a culture bag according to claim 1, characterized in that: Travel wheels (13) are installed at the corner positions of the bottom end of the device platform (1).

3. The negative pressure collection device for cultured suspended cells in a culture bag according to claim 1, characterized in that: An annular groove (11) is provided at the edge of the top of the device platform (1), and a roller (12) is installed at the bottom of the turntable (2) at the position of the annular groove (11), and the roller (12) and the annular groove (11) cooperate with each other.

4. The negative pressure collection device for cultured suspended cells in a culture bag according to claim 1, characterized in that: An annular tube (6) is installed above the vacuum pump (5), and the annular tube (6) is connected to the input end of the vacuum pump (5).

5. The negative pressure collection device for cultured suspended cells in a culture bag according to claim 4, characterized in that: The outer wall of the annular tube (6) is connected to vacuum tubes (21) at equal intervals.

6. The negative pressure collection device for cultured suspended cells in a culture bag according to claim 5, characterized in that: A second flow-stopping clamp (22) is sleeved on the surface of the vacuum tube (21).

7. The negative pressure collection device for cultured suspended cells in a culture bag according to claim 1, characterized in that: The surface of the liquid collecting pipe (19) is provided with a first flow-stopping clamp (20).

8. The negative pressure collection device for cultured suspended cells in a culture bag according to claim 5, characterized in that: The liquid collecting pipe (19) cooperates with the liquid inlet pipe (15), and the vacuum pipe (21) cooperates with the air extraction pipe (16).

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