Multifunctional detection device and multifunctional detection instrument

Abstract

The utility model discloses a multifunctional detection device and a multifunctional detection instrument, and relates to the technical field of biological detection. The multifunctional detection device comprises a detection support, an absorbance detection part, a fluorescence detection part and a detection plate. Wherein the detection support is provided with a sample accommodating hole, an absorbance detection channel and a fluorescence detection channel, and the absorbance detection channel and the fluorescence detection channel are independent from each other; the sample accommodating hole is communicated with the absorbance detection channel and the fluorescence detection channel, and the sample accommodating hole is used for accommodating a cuvette or a test tube seat; the absorbance detection component is arranged in the absorbance detection channel; the fluorescence detection component is arranged in the fluorescence detection channel; the light emitting end of the absorbance detection channel and the light emitting end of the fluorescence detection channel are both located on the first surface of the detection support, and the first surface is covered with the detection plate. Therefore, the device has the advantages that the accuracy of a detection result and the detection reliability are improved, and the overall structure is simpler and more compact.

Description

Technical Field

[0001] The present application relates to the technical field of biological detection, and in particular to a multifunctional detection device and a multifunctional detection instrument. Background Art

[0002] Micro-volume spectrophotometers offer advantages such as low sample volume and a wide measurement concentration range. However, due to structural limitations, they are generally not suitable for measuring extremely low-concentration or volatile samples. To accommodate a wider range of sample types, existing absorbance detection instruments typically combine a micro-volume spectrophotometer with a cuvette detection module. However, due to factors such as fiber optic light guidance and the need for a blank sample, these common cuvette detection modules are complex, bulky, and have limited functionality.

[0003] In certain special detection scenarios in the field of biological testing, such as the detection of E. coli, the detection process includes both sample absorbance detection and sample fluorescence intensity detection. However, simply stacking existing fluorescence detection instruments with absorbance detection instruments not only makes the overall instrument structure complex and too heavy, but also makes it inconvenient to add cuvettes to assist in detection. Therefore, there is an urgent need for a detection device that can accommodate cuvettes and integrates absorbance and fluorescence detection functions in one, with a simple and compact overall structure.

[0004] Based on this, both absorbance and fluorescence detection require a test plate to measure the output light intensity. However, due to individual differences in detection errors between different test plates, the reliability of the comparison, calibration, and analysis of test results is low. Utility Model Content

[0005] The purpose of this application is to provide a multifunctional detection device and a multifunctional detection instrument, which has absorbance detection function and fluorescence detection function, can accommodate a cuvette, and the overall structure of the device is simple and compact, and the accuracy and reliability of the detection results are improved.

[0006] The embodiment of the present application is implemented as follows:

[0007] In a first aspect, an embodiment of the present application provides a multifunctional detection device, comprising: a detection support, an absorbance detection component, a fluorescence detection component, and a detection plate. The detection support comprises a sample receiving hole, an absorbance detection channel, and a fluorescence detection channel, wherein the absorbance detection channel and the fluorescence detection channel are independent of each other; the sample receiving hole is connected to the absorbance detection channel and the fluorescence detection channel, and the sample receiving hole is used to receive a cuvette or a test tube holder; the absorbance detection component is disposed within the absorbance detection channel; the fluorescence detection component is disposed within the fluorescence detection channel; the light output ends of the absorbance detection channel and the fluorescence detection channel are both located on a first surface of the detection support, and the detection plate covers the first surface.

[0008] In combination with the technical solution provided in the first aspect above, in some embodiments, the detection support further has a first slot, which is arranged on one side of the sample accommodating hole and is connected to the sample accommodating hole; a clamping piece is provided in the first slot, and the clamping piece is used to clamp the test tube holder or the cuvette.

[0009] In combination with the technical solution provided in the first aspect above, in some embodiments, the cross section of the sample accommodating hole is square, and the direction from the center of the sample accommodating hole to the vertex of the sample accommodating hole is the clamping direction of the clamping member.

[0010] In combination with the technical solution provided in the first aspect above, in some embodiments, the multifunctional detection device further includes a test tube holder having a test tube accommodating hole, and the cross-sectional outer contour of the test tube holder is the same as that of the sample accommodating hole.

[0011] In combination with the technical solution provided in the first aspect above, in some embodiments, a light-through hole is provided on each side wall and / or each side edge of the test tube holder, and the light-through hole is connected to the test tube accommodating hole; along the extension direction of the sample accommodating hole, the height of the multiple light-through holes relative to the bottom end of the test tube holder is consistent, and is equal to the distance between the fluorescence detection channel and the bottom end of the sample accommodating hole.

[0012] In combination with the technical solution provided in the first aspect above, in some embodiments, the fluorescence detection channel includes an excitation light emission channel and a fluorescence receiving channel, and the extension direction of the excitation light emission channel is perpendicular to the extension direction of the fluorescence receiving channel.

[0013] In combination with the technical solution provided in the first aspect above, in some embodiments, the fluorescence detection component includes an excitation light emitting component and a fluorescence receiving component; the excitation light emitting component includes a first light-emitting component, a first filter and a first lens arranged in an excitation light emission channel, and the first filter is arranged between the first light-emitting component and the first lens; the fluorescence receiving component includes a second lens and a second filter arranged in the fluorescence receiving channel, and the second filter is arranged between the second lens and the detection board.

[0014] In combination with the technical solution provided in the first aspect above, in some embodiments, the absorbance detection channel includes a detection light emitting channel and a detection light receiving channel respectively located on both sides of the sample accommodating hole; the extension directions of the detection light emitting channel and the detection light receiving channel are on the same straight line.

[0015] In combination with the technical solution provided in the first aspect above, in some embodiments, the absorbance detection component includes a detection light emitting component and a detection light receiving component; the detection light emitting component includes a second light-emitting component and a third lens arranged in the detection light emitting channel, and the third lens is arranged on the side of the second light-emitting component close to the sample receiving hole; the detection light receiving component includes a third filter, and the third filter is arranged in the detection light receiving channel.

[0016] In the second aspect, an embodiment of the present application provides a multifunctional detection instrument, which includes: a body and a multifunctional detection device provided by any embodiment of the first aspect of the present application, the multifunctional detection device is arranged in the body, and the top of the sample accommodating hole is provided with a light-shielding cover that can be opened and closed with the body.

[0017] The beneficial effects of this application compared with the prior art are:

[0018] The multifunctional detection device provided in the present application makes the overall structure of the detection device more compact by integrating the absorbance detection component and the fluorescence detection component on the same detection support; wherein, the absorbance detection channel where the absorbance detection component is located is independent of the fluorescence detection channel where the fluorescence detection component is located, but both are connected to the sample accommodating hole, so that the sample accommodating hole is compatible with cuvettes and test tube holders, thereby making the overall structure of the detection device simpler; on this basis, the absorbance detection component and the fluorescence detection component share the same detection board, which can effectively reduce or even eliminate the detection comparison result errors caused by individual differences in the detection boards, improve the accuracy and reliability of the detection results, and can simplify components and reduce costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without creative work.

[0020] Figure 1 This is a schematic structural diagram of a multifunctional detection instrument according to an embodiment of the present application;

[0021] Figure 2 This is a schematic diagram of the overall structure of a multifunctional detection device according to an embodiment of the present application;

[0022] Figure 3 A schematic diagram of a multi-directional cross-section of a multifunctional detection device according to an embodiment of the present application;

[0023] Figure 4 A schematic top view of a multifunctional detection device according to an embodiment of the present application;

[0024] Figure 5 This is a schematic diagram of the overall structure of a multifunctional detection device with a test tube and a test tube holder placed thereon according to an embodiment of the present application;

[0025] Figure 6 This is a cross-sectional schematic diagram of a multifunctional detection device for fluorescence detection according to an embodiment of the present application;

[0026] Figure 7 This is a schematic diagram of the assembly of a test tube and a test tube holder according to an embodiment of the present application;

[0027] Figure 8 This is a schematic front view of a test tube holder according to an embodiment of the present application;

[0028] Figure 9 for Figure 7 The test tube holder is shown in a cross-sectional view at position AA;

[0029] Figure 10 This is a schematic structural diagram of a multifunctional detection device with a cuvette placed therein according to an embodiment of the present application;

[0030] Figure 11 Schematic cross-sectional view of a multifunctional detection device for absorbance detection according to an embodiment of the present application.

[0031] Icons: 1-Multifunctional detection instrument; 10-Main body; 100-Light shielding cover; 2-Multifunctional detection device; 20-Detection support; 200-Sample accommodating hole; 201-First boss; 202-First slot; 203-First surface; 204-Fluorescence detection channel; 2040-Light output end of fluorescence detection channel; 2041-Excitation light emission channel; 2042-Fluorescence receiving channel; 205-Absorbance detection channel; 2050-Light output end of absorbance detection channel; 2051-Detection light emission channel; 2052-Detection light receiving channel; 3-Test tube holder; 30-Fluorescence detection component; 301-Test tube; 302-Light hole; 303-Test tube accommodating Hole; 31-excitation light emitting component; 32-fluorescence receiving component; 311-first light-emitting element; 312-first light source bracket; 313-first filter; 314-first lens bracket; 315-first lens; 316-second lens; 317-second lens bracket; 318-second filter; 319-second filter bracket; 4-cuvette; 40-absorbance detection component; 41-detection light emitting component; 42-detection light receiving component; 411-second light-emitting element; 412-second light source bracket; 413-third lens bracket; 414-third lens; 415-light source retaining ring; 416-third filter; 50-detection plate; 60-clamping part. DETAILED DESCRIPTION

[0032] The terms "first", "second", "third", etc. are only used to distinguish and describe, and do not indicate the order of arrangement, nor can they be understood as indicating or implying relative importance.

[0033] Furthermore, terms such as "horizontal," "vertical," and "overhanging" do not necessarily imply that a component must be absolutely horizontal or overhanging, but rather that it can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," and does not mean that the structure must be completely horizontal, but rather that it can be slightly tilted.

[0034] In the description of this application, it should be noted that the terms "inside", "outside", "left", "right", "up", "down", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, or are the directions or positional relationships in which the product of the application is usually placed when in use. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they should not be understood as limitations on this application.

[0035] In the description of this application, unless otherwise clearly specified and limited, the terms "set", "install", "connected" and "connect" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be a connection between the internal parts of two elements.

[0036] The technical solution of this application will be described in detail below with reference to the accompanying drawings.

[0037] See Figure 1 , Figure 1 This is a schematic diagram of the structure of a multifunctional detection instrument 1 according to an embodiment of the present application. Figure 1 As shown, the embodiment of the present application provides a multifunctional detection instrument 1, which includes a body 10 and a multifunctional detection device 2, wherein the multifunctional detection device 2 is disposed in the body 10. The multifunctional detection device 2 has a sample receiving hole 200, and a light shielding cover 100 is provided on the top of the sample receiving hole 200 so as to be openably connected to the body 10.

[0038] In the embodiment of the present application, the multifunctional detection device 2 has both fluorescence detection and absorbance detection functions. To minimize or even eliminate interference from external light when performing fluorescence or absorbance detection, the above technical solution disposes the multifunctional detection device 2 within the housing 10 and disposes a light shielding cover 100, which is openably connected to the housing 10, on top of the sample receiving hole 200. This effectively shields the multifunctional detection instrument 1 from light while performing detection, thereby improving the detection accuracy of both the multifunctional detection device 2 and the multifunctional detection instrument 1.

[0039] In some embodiments, the light shielding cover 100 can be pivotally connected to the body 10 via a rotating shaft. The light shielding cover 100 can also be configured as a detachable cover, which covers the top of the sample receiving hole 200 when the instrument needs to be sealed and shielded from light.

[0040] See Figures 2 to 3 As shown, Figure 2 This is a schematic diagram of the overall structure of a multifunctional detection device 2 according to an embodiment of the present application; Figure 3 This is a multi-directional cross-sectional diagram of a multifunctional detection device 2 according to an embodiment of the present application. Figures 2 to 3 As shown, an embodiment of the present application provides a multifunctional detection device 2, including: a detection support 20, an absorbance detection component 40, a fluorescence detection component 30 and a detection plate 50.

[0041] The detection support 20 includes a sample receiving hole 200, an absorbance detection channel 205, and a fluorescence detection channel 204. The absorbance detection channel 205 and the fluorescence detection channel 204 are independent of each other and communicate with each other. The sample receiving hole 200 is used to accommodate a cuvette 4 or a test tube holder 3. The absorbance detection component 40 is disposed within the absorbance detection channel 205, and the fluorescence detection component 30 is disposed within the fluorescence detection channel 204. The detection support 20 has a first surface 203. The light output end 2050 of the absorbance detection channel and the light output end 2040 of the fluorescence detection channel are both located on the first surface 203 of the detection support 20. The detection plate 50 covers the first surface 203.

[0042] Since the detection mechanisms of fluorescence detection and absorbance detection are different, the fluorescence detection optical path and the absorbance detection optical path need to be staggered during layout to reduce or even avoid the influence between them. Therefore, the absorbance detection channel 205 and the fluorescence detection channel 204 are independent of each other and have no intersection area. The above technical solution makes the overall structure of the detection device more compact by integrating the absorbance detection component 40 and the fluorescence detection component 30 into the same detection support 20; the absorbance detection channel 205 where the absorbance detection component 40 is located and the fluorescence detection channel 204 where the fluorescence detection component 30 is located are independent of each other, but are both connected to the sample accommodating hole 200, so that the sample accommodating hole 200 can be used for multiple purposes, and is compatible with the cuvette 4 and the test tube holder 3, making the overall structure of the detection device simpler; on this basis, the light output end 2040 of the fluorescence detection channel and the light output end 2050 of the absorbance detection channel are both located on the first surface 203, and the absorbance detection component 40 and the fluorescence detection component 30 share the same detection board 50, which can effectively reduce or even eliminate the detection result deviation caused by individual differences of the detection board 50, improve the accuracy and detection reliability of the detection results, and can further simplify components and reduce costs.

[0043] See Figure 4 , Figure 4 This is a top view of a multifunctional detection device 2 according to an embodiment of the present application. Figure 2 、 Figure 4 As shown, a first boss 201 is provided on the top surface of the detection support 20 , and the sample receiving hole 200 can be provided on the top surface of the first boss 201 and located at the center or edge of the first boss 201 . Figure 4 The sample receiving hole 200 is shown located at the center of the first boss 201. The first boss 201 also includes a first slot 202, which is located to one side of the sample receiving hole 200 and communicates with the sample receiving hole 200. A clamping member 60 is disposed within the first slot 202, which is used to secure the test tube holder 3 or the cuvette 4. By configuring the first slot 202 and the clamping member 60, the sample container within the sample receiving hole 200 can be positioned and secured, thereby assisting the multifunctional detection device 2 in efficiently and reliably completing detection tasks.

[0044] In some embodiments, the depth of the bottom surface of the first groove 202 relative to the top surface of the first boss 201 can be set to be no less than the height of the top surface of the first boss 201 relative to the top surface of the detection support 20. In the above technical solution, the first groove 202 has a certain depth, which helps the clamping member 60 more firmly clamp the sample container, such as the cuvette 4 or the test tube holder 3, in the sample receiving hole 200.

[0045] In some embodiments, the cross-section of the sample receiving hole 200 can be square. The direction from the center of the sample receiving hole 200 to the vertex of the sample receiving hole 200 can be set as the clamping direction F of the clamping member 60. In the embodiment of the present application, the clamping direction of the clamping member 60 is the direction from the center of the sample receiving hole 200 to the vertex. When the cross-sections of the sample receiving hole 200 and the sample container are both square, the two side walls of the sample container can be simultaneously pressed against the inner wall of the sample receiving hole 200, thereby achieving precise positioning and clamping of the sample container.

[0046] During one operation, when the cuvette 4 containing the sample to be tested is inserted into the sample receiving hole 200, the cuvette 4 is subjected to the clamping force of the clamping member 60 (taking the clamping member 60 as a spring sheet as an example, the clamping force is the elastic force of the spring sheet), and is firmly clamped and precisely positioned in the sample receiving hole 200, waiting for the multifunctional detection device 2 to subsequently perform absorbance detection work; when the test tube 301 containing the sample to be tested is combined with the test tube holder 3 and inserted into the sample receiving hole 200, the test tube holder 3 is subjected to the clamping force of the clamping member 60, and is firmly clamped and precisely positioned in the sample receiving hole 200, waiting for the multifunctional detection device 2 to subsequently perform fluorescence detection work.

[0047] See Figure 5 and Figure 6 , Figure 5 This is a schematic diagram of the overall structure of a multifunctional detection device 2 with a test tube 301 and a test tube holder 3 placed thereon according to an embodiment of the present application; Figure 6 This is a cross-sectional diagram of a multifunctional detection device 2 for fluorescence detection according to an embodiment of the present application. Figure 3 、 Figures 5 and 6 As shown, before the multifunctional detection device 2 performs fluorescence detection, the test tube 301 containing the sample to be tested is combined with the test tube holder 3 corresponding to the test tube 301 (for example, PCR test tubes of different capacities can be equipped with different PCR test tube holders), and then inserted into the sample receiving hole 200.

[0048] In some embodiments, the fluorescence detection channel 204 includes an excitation light emission channel 2041 and a fluorescence receiving channel 2042, wherein the excitation light emission channel 2041 extends perpendicularly to the fluorescence receiving channel 2042. In the above technical solution, the excitation light emission channel 2041 is perpendicular to the fluorescence receiving channel 2042, so that the excitation light emission light path is perpendicular to the fluorescence receiving light path, thereby reducing background signal interference and improving detection sensitivity.

[0049] In some embodiments, the fluorescence detection component 30 includes an excitation light emitting component 31 and a fluorescence receiving component 32. The excitation light emitting component 31 is disposed in the excitation light emitting channel 2041, and the fluorescence receiving component 32 is disposed in the fluorescence receiving channel 2042. The excitation light emitting component 31 includes a first light emitting element 311, a first filter 313, and a first lens 315 disposed in the excitation light emitting channel 2041, with the first filter 313 disposed between the first light emitting element 311 and the first lens 315; the fluorescence receiving component 32 includes a second lens 316 and a second filter 318 disposed in the fluorescence receiving channel 2042, with the second filter 318 disposed between the second lens 316 and the detection board 50.

[0050] In some embodiments, the excitation light emission component 31 may also include a first light source bracket 312 and a first lens bracket 314 arranged in the excitation light emission channel 2041, and the first light-emitting element 311 is fixed at the starting end of the excitation light emission channel 2041 through the first light source bracket 312; the first lens 315 can be a plano-convex lens, the convex surface of the first lens 315 is arranged toward the sample accommodating hole 200, and the first lens 315 is fixed at one end of the excitation light emission channel 2041 close to the sample accommodating hole 200 through the first lens bracket 314; the first filter 313 is clamped between the first light source bracket 312 and the first lens bracket 314, and the centers of the first light source bracket 312 and the first lens bracket 314 both have light-transmitting holes for light to pass through.

[0051] In some embodiments, the fluorescence receiving assembly 32 may further include a second lens holder 317 and a second filter holder 319. The second lens 316 may be a biconvex lens, which is fixed to the end of the fluorescence receiving channel 2042 near the sample receiving hole 200 via the second lens holder 317. The second filter 318 is sandwiched between the second lens holder 317 and the second filter holder 319. The centers of the second lens holder 317 and the second filter holder 319 each have a light-transmitting hole for the fluorescence light to pass through.

[0052] During fluorescence detection, the detection light emitted by the first light-emitting element 311 passes through the light-transmitting hole of the first light source holder 312 and reaches the first filter 313. After being filtered out by the first filter 313 to remove the excitation light of a specific wavelength, it passes through the light-transmitting hole of the first lens holder 314. After being converged by the first lens 315, it passes through the light-transmitting hole 302 of the test tube holder 3 and reaches the sample to be tested in the test tube 301. The fluorescent light emitted by the sample to be tested passes through another light-transmitting hole 302 in the test tube holder 3, is converged by the second lens 316, passes through the light-transmitting hole of the second lens holder 317, and after being filtered out by the second filter 318 to remove the light of the target wavelength, it passes through the light-transmitting hole of the second filter holder 319 and reaches the detection board 50. The detection board 50 detects and analyzes the fluorescent light to be tested.

[0053] See Figures 7 to 9 , Figure 7 This is a schematic diagram of the assembly of a test tube 301 and a test tube holder 3 according to an embodiment of the present application; Figure 8 This is a schematic front view of a test tube holder 3 according to an embodiment of the present application; Figure 9 for Figure 7 The test tube holder 3 is shown as a schematic cross-sectional view at AA.

[0054] Please combine Figures 5 to 9 As shown, in some embodiments, the multifunctional detection device 2 further includes a test tube holder 3 having a test tube receiving hole 303. The cross-sectional outer contour of the test tube holder 3 is identical to the cross-sectional outer contour of the sample receiving hole 200. In the above technical solution, the cross-sectional outer contour of the test tube holder 3 is identical to that of the sample receiving hole 200, which facilitates rapid insertion of the test tube holder 3 into the sample receiving hole 200 and accurate positioning and securing. The cross-sectional outer contour of the sample receiving hole 200 is also identical to the cross-sectional outer contour of the cuvette 4.

[0055] In some embodiments, each side wall and / or each side edge of the test tube holder 3 is provided with a light hole 302, and the light hole 302 is connected to the test tube receiving hole 303. Along the extension direction of the sample receiving hole 200, the central axes of the plurality of light holes 302 are at the same height relative to the bottom end of the test tube holder 3, and are equal to the distance between the central axis of the fluorescence detection channel 204 and the bottom end of the sample receiving hole 200. Figures 7 to 9 For example, each side edge of the square test tube holder 3 is provided with a light through hole 302 , so that the light through holes 302 on the test tube holder 3 are arranged symmetrically, and the axes of adjacent light through holes 302 are in a vertical state.

[0056] Please combine Figure 6 As shown, in the above technical solution, the central axes of the multiple light holes 302 are aligned at the same height relative to the bottom of the test tube holder 3 and are equal to the distance between the central axis of the fluorescence detection channel 204 and the bottom of the sample receiving well 200. This allows the excitation light in the fluorescence detection channel 204 to pass through the light holes 302 and enter the sample to be tested, and the fluorescence generated by the sample to be tested to pass through another light hole 302 and enter the fluorescence receiving channel 2042, thus meeting the light transmission requirements of the fluorescence detection optical path A. The light holes 302 provided in the test tube holder 3 are symmetrically designed, and the axes of adjacent light holes 302 are arranged perpendicularly. This allows the test tube holder 3 to be directly installed in the sample receiving well 200 without distinguishing the orientation, adapting to the mutually perpendicular laser emission channel and fluorescence receiving channel 2042.

[0057] In the embodiment of the present application, the depth of the central axis of the fluorescence detection channel 204 relative to the top surface of the detection support 20 can meet the following conditions: after the test tube holder 3 containing the test tube 301 is loaded into the sample receiving hole 200, the central axis of the fluorescence detection channel 204 should pass through the middle position of the sample to be tested in the test tube 301 as much as possible.

[0058] In some embodiments, the test tube holder 3 further includes a light shield, which is movably mounted on the top of the test tube holder 3. After the test tube 301 containing the sample to be tested is inserted into the test tube receiving hole 303, the light shield can be moved to the top of the test tube receiving hole 303 and completely cover it to reduce the influence of stray light. The light shield can be connected to the test tube holder 3 in the form of a flip cover, a sliding cover, a rubber stopper, etc.

[0059] See Figures 10 and 11 , Figure 10 This is a schematic structural diagram of a multifunctional detection device 2 with a cuvette 4 placed therein according to an embodiment of the present application; Figure 11 This is a cross-sectional diagram of a multifunctional detection device 2 for absorbance detection according to an embodiment of the present application. Figure 3 、 Figures 10 and 11 As shown, before the multifunctional detection device 2 performs absorbance detection, the cuvette 4 containing the sample to be tested is inserted into the sample containing hole 200 .

[0060] In some embodiments, the absorbance detection channel 205 includes a detection light emitting channel 2051 and a detection light receiving channel 2052, respectively located on either side of the sample receiving hole 200. The absorbance detection component 40 includes a detection light emitting assembly 41 and a detection light receiving assembly 42. The detection light emitting assembly 41 is disposed within the detection light emitting channel 2051, and the detection light receiving assembly 42 is disposed within the detection light receiving channel 2052. The extension directions of the detection light emitting channel 2051 and the detection light receiving channel 2052 are on the same straight line. In the above technical solution, in the absorbance detection channel 205, the central axes of the emitting channel and the receiving channel are on the same straight line, which can improve the accuracy of absorbance measurement and simplify the instrument structure.

[0061] In some embodiments, the detection light emitting component 41 may include a second light-emitting component 411 and a third lens 414, and the third lens 414 is arranged on a side of the second light-emitting component 411 close to the sample receiving hole 200. The detection light receiving component 42 may include a third filter 416, and the third filter 416 is arranged in the detection light receiving channel 2052.

[0062] In some embodiments, the detection light emitting assembly 41 may further include a second light source bracket 412, a third lens bracket 413, and a light source retaining ring 415. The second light emitting element 411 is fixed to the starting end of the detection light emitting channel 2051 via the second light source bracket 412. The third lens 414 may be a biconvex lens, sandwiched between the third lens bracket 413 and the light source retaining ring 415. Both the third lens bracket 413 and the light source retaining ring 415 have light-transmitting holes at their centers.

[0063] During absorbance detection, the detection light emitted by the second light-emitting element 411 enters the third lens 414 through the light-transmitting hole of the third lens holder 413. The detection light, focused by the third lens 414, passes through the light-transmitting hole of the light source retaining ring 415 and enters the sample to be tested in the cuvette 4. After the sample to be tested in the cuvette 4 absorbs some of the light, the remaining light is filtered by the third filter 416, resulting in light to be tested at a specific wavelength. The light to be tested then reaches the detection board 50, where it is detected and analyzed.

[0064] In the embodiment of the present application, the depth of the central axis B of the absorbance detection channel 205 relative to the bottom of the sample receiving hole 200 can meet the following conditions: after the cuvette 4 is inserted into the sample receiving hole 200, the central axis of the absorbance detection channel 205 should pass through the middle of the sample to be tested in the cuvette 4 as much as possible, and the sample to be tested in the cuvette 4 generally occupies about half of the volume of the cuvette 4. For example, if the height of the cuvette 4 is approximately 45 mm, the central axis B of the absorbance detection channel 205 should be set within a height range of 8 mm to 12 mm from the bottom of the cuvette 4 (or the sample receiving hole 200).

[0065] The multifunctional detection device 2 provided in the embodiment of the present application has a compact overall structure and occupies a small space. It can realize the absorbance detection function and the fluorescence detection function based on a sample accommodating hole 200, and efficiently utilize the space within the detection support 20 and the detection instrument; the absorbance detection component 40 and the fluorescence detection component 30 can be customized with different light-emitting parts and filters to adapt to the detection requirements of light of different wavelengths, thereby improving the practicality and ease of use of the multifunctional detection device 2.

[0066] The above description is merely a preferred embodiment of the present application and is not intended to limit the present application. Various modifications and variations are possible for those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. A multifunctional detection device, characterized in that: include: A detection support, wherein the detection support has a sample accommodating hole, an absorbance detection channel, and a fluorescence detection channel, wherein the absorbance detection channel and the fluorescence detection channel are independent of each other; the sample accommodating hole is connected to the absorbance detection channel and the fluorescence detection channel, and the sample accommodating hole is used to accommodate a cuvette or a test tube holder; an absorbance detection component, the absorbance detection component being disposed in the absorbance detection channel; a fluorescence detection component, the fluorescence detection component being disposed in the fluorescence detection channel; The detection plate, the light output end of the absorbance detection channel and the light output end of the fluorescence detection channel are both located on the first surface of the detection support, and the detection plate covers the first surface.

2. The multifunctional detection device according to claim 1, characterized in that: The detection support further has a first slot, which is arranged on one side of the sample accommodating hole and communicated with the sample accommodating hole; a clamping piece is provided in the first slot, and the clamping piece is used to clamp the test tube holder or the cuvette.

3. The multifunctional detection device according to claim 2, characterized in that: The cross section of the sample accommodating hole is square, and the direction from the center of the sample accommodating hole to the vertex of the sample accommodating hole is the clamping direction of the clamping member.

4. The multifunctional detection device according to claim 1, characterized in that: The multifunctional detection device further comprises the test tube holder, which has a test tube accommodating hole. The cross-sectional outer contour of the test tube holder is the same as that of the sample accommodating hole.

5. The multifunctional detection device according to claim 4, characterized in that: Each side wall and / or each side edge of the test tube holder is provided with a light-through hole, and the light-through hole is communicated with the test tube accommodating hole; Along the extension direction of the sample accommodating hole, the heights of the plurality of light holes relative to the bottom end of the test tube holder are consistent and are equal to the distance between the fluorescence detection channel and the bottom end of the sample accommodating hole.

6. The multifunctional detection device according to claim 1, characterized in that: The fluorescence detection channel includes an excitation light emission channel and a fluorescence receiving channel, and an extension direction of the excitation light emission channel is perpendicular to an extension direction of the fluorescence receiving channel.

7. The multifunctional detection device according to claim 6, characterized in that: The fluorescence detection component includes an excitation light emitting component and a fluorescence receiving component; The excitation light emission component includes a first light emitting element, a first filter and a first lens arranged in the excitation light emission channel, wherein the first filter is arranged between the first light emitting element and the first lens; The fluorescence receiving component includes a second lens and a second filter arranged in the fluorescence receiving channel, and the second filter is arranged between the second lens and the detection board.

8. The multifunctional detection device according to claim 1, characterized in that: The absorbance detection channel includes a detection light emitting channel and a detection light receiving channel respectively located on both sides of the sample accommodating hole; and the extension directions of the detection light emitting channel and the detection light receiving channel are on the same straight line.

9. The multifunctional detection device according to claim 8, characterized in that: The absorbance detection component includes a detection light emitting component and a detection light receiving component; The detection light emitting assembly includes a second light emitting element and a third lens disposed in the detection light emitting channel, wherein the third lens is disposed on a side of the second light emitting element close to the sample receiving hole; The detection light receiving component includes a third filter, and the third filter is arranged in the detection light receiving channel.

10. A multifunctional detection instrument, characterized in that: The multifunctional detection instrument comprises: body; The multifunctional detection device according to any one of claims 1 to 9, wherein the multifunctional detection device is arranged in the body, and a light shielding cover that is openably connected to the body is provided on the top of the sample accommodating hole.

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