Kit and synthesis device for synthesizing Al18F-NOTA-LM3

Abstract

The utility model belongs to the field of medicine synthesis, and particularly relates to a kit and a synthesis device for synthesizing Al18F-NOTA-LM3, the device comprises a reaction component and a purification component, and the reaction component is connected with the purification component; the reaction assembly comprises an < 18 > F <-> receiving part, a reactor and a plurality of reaction liquid storages, the < 18 > F <-> receiving part is communicated with the reactor and used for guiding synthesized < 18 > F <-> into the reactor, and the reaction liquid storages are communicated with the reactor and used for guiding reaction liquid into the reactor; the < 18 > F <-> receiving part is used for receiving < 18 > F <-> generated by an external synthesizer, the purification assembly comprises a purification column, a product storage bottle and a plurality of purification bottles, the purification column is used for guiding a reacted solution into the purification column, and the purification bottles are filled with purification liquid and used for guiding the purification liquid into the purification column and eluting the reacted solution in the purification column; and the product storage bottle is used for storing the purified reaction liquid. The device disclosed by the utility model can be used for obtaining Al18F-NOTA-LM3 of which the radiochemical purity is greater than 95%.

Description

Technical Field

[0001] The utility model belongs to the field of drug synthesis, in particular to a method for synthesizing Al 18 Kit and synthesis device for F-NOTA-LM3. Background Art

[0002] 18 F has a half-life of 109 minutes, emits 100% β+ rays with a short range, and has excellent physical properties and can be obtained in large quantities from accelerator production. 18 F - The advantages of radioactive labeling enable it to provide sufficient products to meet the imaging needs of more cancer patients and gradually replace the current positron-emitting radionuclides. 68 Ga-labeled somatostatin analogs are used in imaging of neuroendocrine tumors.

[0003] Somatostatin is widely distributed in the central endocrine system and extracerebral tissues. It is an important cyclic peptide hormone family that regulates tissue endocrine and endocrine secretion. It exerts its effects by binding to SSTRs (SSTRs) in target cells. Some SSTRs are often overexpressed in tumor tissues, such as neuroendocrine tumors (gastroenteropancreatic neuroendocrine tumors, pituitary tumors, pheochromocytomas, etc.) and nervous system tumors (neuroblastomas, meningiomas, etc.). Studies have shown that somatostatin and its analogs have anti-tumor activity, not only arresting the tumor cell cycle and inducing tumor cell apoptosis, but also inhibiting tumor angiogenesis and antagonizing its growth-promoting effects.

[0004] 18 The F-AlF method has been used to prepare a variety of somatostatin receptor-targeting 18 F-triazacyclononadecyldiacetyl-1-naphthylalanine-octreotide (18F-AlF-NOTA-NOC) and targeting integrin α v β3 18 F-triazacyclononadecyl triacetate-triethanolamine-cyclic divalent arginine-glycine-aspartic acid ( 18 F-AlF-NOTA-PEG3-Glu-RGD2), 18 F-AlF-NOTANOC and 18 However, most existing methods use kits to prepare low-dose 18 F-AlF-NOTA-biomolecules are difficult to achieve industrial production, and the corresponding device structure is relatively complex, resulting in low purity and stability of the obtained products. Utility Model Content

[0005] In order to improve the deficiencies of the prior art, the present invention provides a method for synthesizing Al 18 The kit and synthesis device of F-NOTA-LM3 have simple structure and can synthesize Al with radiochemical purity greater than 95%. 18 F-NOTA-LM3.

[0006] In the first aspect, the utility model provides a method for synthesizing Al 18 The F-NOTA-LM3 test kit includes a box body, which is equipped with an eluent storage bottle, a recovery bottle, a product storage bottle, a reaction liquid storage bottle and several purification bottles.

[0007] According to an embodiment of the present invention, the eluent is used to store eluent, such as physiological saline.

[0008] According to an embodiment of the present invention, the reaction liquid storage is used to store the reaction liquid, and the reaction liquid storage includes several reaction liquid storage bottles.

[0009] According to the embodiment of the present utility model, the reaction liquid storage device includes a first reaction liquid storage bottle, a second reaction liquid storage bottle and a third reaction liquid storage bottle. Preferably, the first reaction liquid storage bottle stores 200ug reaction precursor, 300ul0.2N glacial acetic acid and 1mL ACN, the second reaction liquid storage bottle stores 3ml AlCl3, and the third reaction liquid storage bottle stores 600mg NaAsc.

[0010] According to the embodiment of the present invention, the third reaction liquid storage bottle is connected to a container with physiological saline. When needed, physiological saline is introduced into the third reaction liquid storage bottle to form a NaAsc solution with NaAsc, and then introduced into the reactor to prevent NaAsc from staying in the solution state for too long and deteriorating.

[0011] According to an embodiment of the present invention, the purification bottle includes four purification bottles: a first purification bottle, a second purification bottle, a third purification bottle and a fourth purification bottle.

[0012] According to the embodiment of the present invention, the first purification bottle contains 50% ethanol, the second purification bottle contains 600 mg of NaAsc, the third purification bottle contains physiological saline, and the fourth purification bottle contains anhydrous ethanol.

[0013] In the second aspect, the utility model provides a method for synthesizing Al 18 The F-NOTA-LM3 device comprises a reaction component and a purification component, wherein the reaction component and the purification component are interconnected;

[0014] The reaction component includes 18 F -Receiving components, reactors and several reaction liquid storages, the 18 F - The receiving part is connected to the reactor for 18 F - The reaction liquid storage is connected to the reactor and is used to introduce the reaction liquid into the reactor. The reactor is arranged in a temperature controller, and the temperature controller is used to provide the temperature required for the reaction in the reactor.

[0015] According to the embodiment of the present utility model, the 18 F - The receiving component is used to receive the 18 F - The synthesizer is, for example, a cyclotron, in which protons are used to bombard H2 18 O, causing a nuclear reaction of oxygen-18 to produce 18 F - , the nuclear reaction is 18 O(p,n) 18 F.

[0016] According to the embodiment of the present utility model, the 18 F - The receiving part is connected to the reactor through an extraction column, and the injection end of the extraction column is connected to the 18 F - Accepting component connection, the extraction column is selected to be able to separate 18 F - extraction column.

[0017] According to the embodiment of the present utility model, the extraction column is made of 4-(4-methylpiperidinyl)pyridine anion exchange resin.

[0018] According to the embodiment of the present utility model, the 18 F - The receiving component is a fluoride ion collector, which is used to collect external fluoride 18 F - synthesized by synthesizer 18 F - , generated from an external cyclotron 18 F - After passing through the fluoride ion collector and then into the 4-(4-methylpiperidinyl)pyridine anion exchange resin, 18 F - It was adsorbed on 4-(4-methylpiperidinyl)pyridine anion exchange resin (QMA separation column).

[0019] According to the embodiment of the present invention, the extraction column is further connected to a rinsing bottle, which is connected to the top of the extraction column through a first syringe. The first syringe is used to draw the rinsing liquid in the rinsing bottle and inject it into the top of the extraction column to elute the extraction column. The extraction column is eluted by H2 18 The O recovery pipeline guides the eluted wastewater into the recovery bottle, and the purified 18 F - Introduce it into the reactor through the reaction tube.

[0020] According to an embodiment of the present invention, the reaction liquid storage is used to store the reaction liquid, and the reaction liquid storage includes a first reaction liquid storage bottle, a second reaction liquid storage bottle and a third reaction liquid storage bottle.

[0021] According to an embodiment of the present invention, the first reaction liquid storage bottle is used to store the precursor solution, the second reaction liquid storage bottle is used to store the AlCl3 solution, and the third reaction liquid storage bottle is used to store the solid NaAsc. The first reaction liquid storage bottle, the second reaction liquid storage bottle, and the third reaction liquid storage bottle use a second syringe to introduce the corresponding reaction liquid into the reactor through a connecting pipe. According to an embodiment of the present invention, the first reaction liquid storage bottle, the second reaction liquid storage bottle, and the third reaction liquid storage bottle are all provided with a syringe access port, and the second syringe is provided with an extraction tube, which can be inserted into the syringe access port to extract the corresponding reaction liquid.

[0022] According to the implementation scheme of the present utility model, the purification component includes a purification column, a product storage bottle and several purification bottles. The purification column is connected to the reactor through a connecting pipe. The connecting pipe is used to introduce the reacted solution into the purification column. The purification bottle and the product storage bottle are both connected to the purification column, and the purification bottle is filled with purified liquid.

[0023] According to the embodiment of the present invention, the purification bottle includes a first purification bottle, a second purification bottle, a third purification bottle and a fourth purification bottle, wherein the first purification bottle is used to store 50% ethanol solution, the second purification bottle is used to store solid NaAsc, the third purification bottle is used to store aqueous solution, and the fourth purification bottle is used to store anhydrous ethanol.

[0024] According to the embodiment of the present invention, the second purification bottle is connected to the third purification bottle through a connecting pipe. After the first purification bottle elutes the purification column, the saline in the third purification bottle is injected into the second purification bottle to form a NaAsc solution, which is then introduced into the purification column for elution.

[0025] According to the embodiment of the present utility model, the first purification bottle, the second purification bottle, the third purification bottle and the fourth purification bottle are connected to the purification column through a connecting pipe, and the corresponding purification liquid is introduced into the top of the purification column to elute the reacted solution. The bottom of the purification column is connected to the product storage bottle through a connecting pipe for introducing the purified product into the product storage bottle.

[0026] According to the embodiment of the present invention, the device further comprises H2 18 O recovery device.

[0027] Beneficial effects

[0028] The synthesis device of the utility model is not only simple in structure, but also can be synthesized by the synthesizer. 18 F - After being filtered through the extraction column, it is introduced into the reactor and then reacted with other reaction solutions. The reaction solution is purified inside the device and fully purified through multiple purification solutions stored in independent purification bottles: 50% ethanol solution, solid NaAsc, physiological saline and anhydrous ethanol. Not only can Al with a radiochemical purity greater than 95% be obtained, 18 F-NOTA-LM3, and the product has low residual radioactivity and high stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 For the synthesis of Al 18 Schematic diagram of the structure of the F-NOTA-LM3 device;

[0030] Figure 2 Al prepared in Example 1 18 Radioactive thin-layer chromatogram of F-NOTA-LM3.

[0031] In the figure, 1-recovery bottle, 2-rinsing bottle, 3-first syringe, 4-temperature controller, 5-extraction column, 6-fluoride ion collector, 7-reaction tube, 8-second syringe, 9-first reaction liquid storage bottle, 10-second reaction liquid storage bottle, 11-third reaction liquid storage bottle, 12-reactor, 13-purification column, 14-first purification bottle, 15-second purification bottle, 16-third purification bottle, 17-fourth purification bottle, 18-product storage bottle. DETAILED DESCRIPTION

[0032] The following will further explain the structure of the present invention in detail with reference to specific embodiments. It should be understood that the following embodiments are merely exemplary illustrations and explanations of the present invention and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above-mentioned content of the present invention are included within the scope of protection intended by the present invention.

[0033] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limitations on the present invention.

[0034] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0035] Example 1

[0036] A method for synthesizing Al 18 The F-NOTA-LM3 test kit includes a box body, which is provided with an eluent storage bottle, a recovery bottle 1, a product storage bottle 18, a reaction liquid storage and several purification bottles.

[0037] The eluent storage bottle is used to store eluent, such as 0.5 ml of physiological saline; the reaction liquid storage device is used to store reaction liquid. The reaction liquid storage device includes several reaction liquid storage bottles. The number of reaction liquid storage bottles is determined according to the type of reaction liquid required for the reaction. In this embodiment, the reaction liquid storage device includes a first reaction liquid storage bottle 9, a second reaction liquid storage bottle 10 and a third reaction liquid storage bottle 11. The first reaction liquid storage bottle 9 stores 200 ug of reaction precursor (NOTA-LM3), 300 ul of 0.2 N glacial acetic acid and 1 mL of ACN, the second reaction liquid storage bottle 10 stores 3 ml of AlCl3, and the third reaction liquid storage bottle 11 stores 600 mg of NaAsc.

[0038] The number of purification bottles is determined according to the type of purification agent. In this embodiment, four purification bottles are included: a first purification bottle 14, a second purification bottle 15, a third purification bottle 16, and a fourth purification bottle 17. The first purification bottle 14 contains 50% ethanol, the second purification bottle 15 contains 600 mg of NaAsc, the third purification bottle 16 contains physiological saline, and the fourth purification bottle 17 contains anhydrous ethanol.

[0039] Example 2

[0040] See also Figure 1A method for synthesizing Al 18 The F-NOTA-LM3 device includes a reaction component and a purification component, and the reaction component and the purification component are connected to each other.

[0041] The reaction component includes 18 F - Receiving component, reactor 12 and reaction liquid storage, the 18 F - The receiving part is connected to the reactor 12 and is used to receive the 18 F - The reaction liquid storage is connected to the reactor 12 and is used to introduce the reaction liquid into the reactor 12. The reactor 20 is set in the temperature controller 4, and the temperature controller 4 is used to provide the temperature required for the reaction of the reactor 12.

[0042] The external synthesizer is, for example, a cyclotron in which protons are used to bombard H2 18 O, leading to 18 O is produced by nuclear reaction 18 F - , the nuclear reaction is 18 O(p,n) 18 F.

[0043] in, 18 F - The receiving component includes a fluoride ion collector 6, which is connected to the reactor 18 through an extraction column 5. The injection end of the fluoride ion collector 6 is connected to an external synthesizer, and the output end is connected to the fluoride ion collector 6. The extraction column 5 is selected from a material capable of separating 18 F - In this embodiment, the extraction column 5 uses 4-(4-methylpiperidinyl) pyridine anion exchange resin (QMA separation column), wherein the extraction column 5 is connected to the external fluoride ion collector 6. 18 F - The synthesizer is connected, and the fluoride ion collector 6 is used to collect external 18 F - synthesized by synthesizer 18 F - , produced from a cyclotron 18 F - After passing through the fluoride ion collector 6, it enters and passes through the 4-(4-methylpiperidinyl)pyridine anion exchange resin (QMA separation column). 18 F - The sample is adsorbed on the QMA separation column. The injection end of the QMA separation column is also connected to a gas inlet pipe (not shown in the figure). The gas inlet pipe is used to introduce elution gas (such as N2) into the QMA separation column to achieve 18 F- eluted to obtain the purified 18 F - And enter the reactor 12, the extraction column 5 is connected to the reactor 12 through a pipeline (the pipeline is not shown).

[0044] The extraction column 5 is also connected to a washing bottle 2, which is connected to the top of the extraction column 5 through a first syringe 3. The first syringe 3 is used to absorb the washing liquid in the washing bottle 2 and inject it into the top of the extraction column 5 to elute the extraction column 5. The extraction column 5 is cooled by H2 18 The O recovery pipeline guides the eluted wastewater into the recovery bottle 1, and the purified 18 F - The liquid is introduced into the reactor 12 through the reaction tube 7 , the elution bottle 2 and the first syringe 3 are connected via a pipeline, and the first syringe 3 and the extraction column 5 are connected via a pipeline (the pipeline is not shown).

[0045] The reaction liquid storage is used to store reaction liquid or other reactants in other states such as solid state. The number of reaction liquid storage bottles is determined according to the type of storage liquid required for the actual reaction. In this embodiment, the reaction liquid storage includes a first reaction liquid storage bottle 9, a second reaction liquid storage bottle 10 and a third reaction liquid storage bottle 11. The first reaction liquid storage bottle 9 is used to store the precursor solution, the second reaction liquid storage bottle 10 is used to store the AlCl3 solution, and the third reaction liquid storage bottle 11 is used to store the solid NaAsc. The first reaction liquid storage bottle 9, the second reaction liquid storage bottle 10 and the third reaction liquid storage bottle 11 are connected by the first reaction liquid storage bottle 9, the second reaction liquid storage bottle 10 and the third reaction liquid storage bottle 11. The two syringes 8 introduce the corresponding reaction liquid into the reactor 12 through the connecting pipe, wherein the first reaction liquid storage bottle 9, the second reaction liquid storage bottle 10 and the third reaction liquid storage bottle 11 are all provided with syringe access ports, and the second syringe 8 is provided with several extraction tubes (not shown in the figure), one end of the extraction tube can be inserted into the access port of the second syringe 8, and the other end is inserted into the corresponding reaction liquid storage bottle. The second syringe 8 extracts the corresponding reaction liquid through the extraction tube. The number of extraction tubes can be one or more, for example, 3. The extracted reaction liquid is introduced into the reactor 12 through the reaction tube 7.

[0046] The third reaction liquid storage bottle 11 is connected to a container with physiological saline. When needed, the physiological saline is introduced into the third reaction liquid storage bottle 11 to form a NaAsc solution with NaAsc, and then introduced into the reactor 12 to prevent NaAsc from staying in the solution state for too long and deteriorating. The purification component includes a purification column 13, a product storage bottle 18 and several purification bottles. The purification column 13 is connected to the reactor 12 through a connecting pipe. The connecting pipe is used to introduce the reacted solution into the purification column 13. The purification bottle and the product storage bottle 18 are both connected to the purification column 13. The purification bottle is filled with purified liquid. The number of purification bottles is set according to actual needs. In this embodiment, the purification bottle includes a first purification bottle 14, a second purification bottle 15, a third purification bottle 16 and a fourth purification bottle 17, wherein the first purification bottle 14 is used to store 50% ethanol solution, the second purification bottle 15 is used to store solid NaAsc, the third purification bottle 16 is used to store aqueous solution (physiological saline), and the fourth purification bottle 17 is used to store Anhydrous ethanol is added. The first purification bottle 14, the second purification bottle 15, the third purification bottle 16, and the fourth purification bottle 17 are connected to the purification column 13 via a connecting pipe (not shown). The corresponding purified solution is introduced into the top of the purification column 13 to elute the reacted solution. The bottom of the purification column 13 is connected to the product storage bottle 18 via a connecting pipe (not shown) for introducing the purified product into the product storage bottle 18. The second purification bottle 15 and the third purification bottle 16 are connected via a connecting pipe. After the first purification bottle 14 elutes the purification column 13, the saline in the third purification bottle 16 is injected into the second purification bottle 15 to form a NaAs solution, which is then introduced into the purification column 13 for elution.

[0047] Example 3

[0048] The apparatus in Example 2 was used, and the reaction conditions in the reactor 12 were 100°C and the reaction time was 15 min to generate a crude product Al 18 The synthetic route of F-NOTA-LM3 is as follows:

[0049]

[0050] First, rinse the purification column 13 with the injection water in the third purification bottle 16, then rinse the product on the purification column 13 with 50% ethanol in the first purification bottle 14, pass the product through a sterile filter membrane into the product storage bottle 18, inject 15ml of normal saline into the second purification bottle 1, form a solution with solid NaAsc and rinse the residual product on the purification column 13, pass the product through a sterile filter membrane into the product storage bottle 18, and finally obtain sterile, clear, radiochemical purity> 95%, and ethanol concentration less than 10% Al 18 For F-NOTA-LM3 injection, use the anhydrous ethanol in the fourth purification bottle 17 to rinse the HLB column, the connecting pipe and the reaction tube to reduce the residual radioactivity therein.

[0051] Example 4

[0052] Al 18 Determination of radiochemical purity of F-NOTA-LM3 injection:

[0053] The Al content was determined by integrated thin layer chromatography (iTLC). 18 The radiochemical purity of F-NOTA-LM3 was determined using iTLC-silica gel (SG) as the stationary phase and V (1 mol / L ammonium acetate): V (methanol) = 1:1 as the developing solvent, according to Appendix XI of the Pharmacopoeia of the People's Republic of China (Volume 4) (2020 edition), see Figure 2 As shown, it is Al 18 Radioactive thin layer chromatogram of F-NOTA-LM3 product, Al 18 F-NOTA-LM3 Product R f The value was 0.63, and the radiochemical purity was greater than 95%.

[0054] The above examples illustrate the specific implementation methods of the present invention. However, the scope of protection of the present invention is not limited to the above-mentioned exemplary implementation methods. Any modifications, equivalent substitutions, improvements, etc. made by those skilled in the art within the spirit and principles of the present invention shall be included in the scope of protection of the claims of the present invention.

Claims

1. A method for synthesizing Al 18 The F-NOTA-LM3 device is characterized in that It includes a reaction component and a purification component, wherein the reaction component and the purification component are connected to each other; The reaction component includes 18 F - Receiving components, reactors and several reaction liquid storages, the 18 F - The receiving part is connected to the reactor for 18 F - The reaction liquid storage is connected to the reactor and is used to introduce the reaction liquid into the reactor. The reactor is set in a temperature controller, and the temperature controller is used to provide the temperature required for the reaction in the reactor; described 18 F - The receiving component is used to receive the 18 F - The reaction liquid storage device is used to store the reaction liquid. The purification component includes a purification column, a product storage bottle and several purification bottles. The purification column is connected to the reactor through a connecting pipe and is used to introduce the reacted solution into the purification column. The purification bottle and the product storage bottle are both connected to the purification column. The purification bottle is filled with purified liquid, which is used to introduce the purified liquid into the purification column to elute the reacted solution in the purification column. The product storage bottle is used to store the purified reaction liquid.

2. The method for synthesizing Al according to claim 1 18 The F-NOTA-LM3 device is characterized in that described 18 F - The receiving part is connected to the reactor through an extraction column, and the injection end of the extraction column is connected to the 18 F - Accepting component connection, the extraction column is selected to be able to separate 18 F - extraction column.

3. The method for synthesizing Al according to claim 2 18 The F-NOTA-LM3 device is characterized in that The extraction column is also connected to a rinsing bottle, which is connected to the top of the extraction column through a first syringe. The first syringe is used to absorb the rinsing liquid in the rinsing bottle and inject it into the top of the extraction column to elute the extraction column. The extraction column is eluted by H2 18 The O recovery pipeline guides the eluted wastewater into the recovery bottle, and the purified 18 F - Introduce it into the reactor through the reaction tube.

4. The method for synthesizing Al according to claim 1 18 The F-NOTA-LM3 device is characterized in that The reaction liquid storage comprises a first reaction liquid storage bottle, a second reaction liquid storage bottle and a third reaction liquid storage bottle; The first reaction liquid storage bottle is used to store the precursor solution, the second reaction liquid storage bottle is used to store the AlCl3 solution, and the third reaction liquid storage bottle is used to store the solid NaAsc. The first reaction liquid storage bottle, the second reaction liquid storage bottle and the third reaction liquid storage bottle use the second syringe to introduce the corresponding reaction liquid into the reactor through the connecting pipe.

5. The method for synthesizing Al according to any one of claims 1 to 4. 18 The F-NOTA-LM3 device is characterized in that The purification bottle includes a first purification bottle, a second purification bottle, a third purification bottle and a fourth purification bottle, wherein the first purification bottle is used to store 50% ethanol solution, the second purification bottle is used to store solid NaAsc, the third purification bottle is used to store physiological saline, and the fourth purification bottle is used to store anhydrous ethanol.

6. The method for synthesizing Al according to claim 5 18 The F-NOTA-LM3 device is characterized in that The first purification bottle, the second purification bottle, the third purification bottle and the fourth purification bottle are connected to the purification column through a connecting pipe, and the corresponding purification liquid is introduced into the top of the purification column to elute the reacted solution. The bottom of the purification column is connected to the product storage bottle through a connecting pipe to introduce the purified product into the product storage bottle.

7. The method for synthesizing Al according to claim 2 18 The F-NOTA-LM3 device is characterized in that The device also includes a recovery bottle, which is connected to the extraction column and is used to store waste water generated by extraction with the extraction column.

8. A method for synthesizing Al according to any one of claims 1 to 7 18 The kit of the F-NOTA-LM3 device comprises a box body, characterized in that: The box body is provided with an eluent storage bottle, a recovery bottle, a product storage bottle, a reaction liquid storage bottle and several purification bottles; The eluent is used to store eluent, and the reaction liquid storage is used to store reaction liquid. The reaction liquid storage includes a plurality of reaction liquid storage bottles.

9. The kit according to claim 8, characterized in that The reaction liquid storage bottle includes a first reaction liquid storage bottle, a second reaction liquid storage bottle and a third reaction liquid storage bottle. The first reaction liquid storage bottle stores 200ug reaction precursor, 300ul 0.2N glacial acetic acid and 1mL ACN, the second reaction liquid storage bottle stores 3mlAlCl3, and the third reaction liquid storage bottle stores 600mg NaAsc.

10. The kit according to claim 8, characterized in that The purification bottle includes four purification bottles: a first purification bottle, a second purification bottle, a third purification bottle and a fourth purification bottle. The first purification bottle stores 50% ethanol, the second purification bottle stores 600 mg of NaAsc, the third purification bottle stores physiological saline, and the fourth purification bottle stores anhydrous ethanol.

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