Full-automatic sampling equipment of bioreactor

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The fully automated bioreactor sampling equipment solves the problems of cumbersome manual sampling operations and high contamination risks in the existing technology, and achieves pollution-free, accurate multi-sample sampling and sample preservation, which is suitable for various bioreactors.

CN223316681UActive Publication Date: 2025-09-09WUHAN CEKG TECH CO LTD

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Patent Information

Application Number: CN202422200391.3
Authority / Receiving Office: CN · China
Patent Type: Utility models(China)
Current Assignee / Owner
Filing Date: 2024-09-09
Publication Date: 2025-09-09
Estimated Expiration: 2034-09-09

AI Technical Summary

Technical Problem

The existing bioreactor sampling process requires manual timed operation, which is cumbersome to operate, has a high risk of contamination and is difficult to achieve multiple sampling. It is especially inconvenient when multiple sampling is required at night. The sampling volume varies, and sample preservation requires additional equipment.

Method used

A fully automatic sampling device for bioreactors was designed, which includes a control cabinet, sampling pipeline components, a peristaltic pump, a tube clamp valve and a touch screen. It enables simultaneous sampling of multiple samples through automated control, uses disposable sterile supplies to reduce the risk of contamination, and is equipped with a refrigeration component to preserve samples.

Benefits of technology

It realizes convenient, pollution-free and efficient multi-sample sampling, reduces manual intervention, has strong compatibility, is suitable for any bioreactor, and can realize automatic refrigerated storage of samples.

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Abstract

The utility model discloses full-automatic sampling equipment for a bioreactor, which comprises a control cabinet, at least one set of sampling pipeline component used for being connected with a sampling container and at least one group of pipe clamp valves used for controlling the on-off of the sampling pipeline component, and the at least one group of pipe clamp valves is electrically connected with a controller arranged in the control cabinet. The device has the advantages that the compatibility is good, and the device can be connected with any bioreactor; the operation is convenient, and the whole sampling process is automatically carried out; high throughput is achieved, multiple samples can be sampled at a time, one sample can also be sampled at a time, and sampling is continuously conducted for multiple times; sterile sampling is achieved, cleaning is not needed, and the whole sampling pipeline assembly is a disposable sterile article; the sampling tube has a refrigeration function, so that further reaction of the sample in the sampling tube due to high temperature is avoided after sampling.

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Description

Technical Field

[0001] The utility model relates to the technical field of bioreactor sampling, in particular to a full-automatic sampling device for a bioreactor. Background Art

[0002] Existing bioreactors typically use manual sampling and timed sampling. Sampling requires a syringe or connection to a tube adapter or tube sealer. Each operation can only take one sample, which is cumbersome and carries a high risk of contamination. Multiple sampling operations at night require manual timed sampling, and the amount of samples taken fluctuates. Furthermore, when samples need to be stored, separate refrigeration equipment is required. Utility Model Content

[0003] In view of the problems existing in the background technology, the purpose of the present invention is to provide a fully automatic sampling device for a bioreactor which is easy to operate, pollution-free, and can accurately sample multiple samples at a time.

[0004] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0005] A fully automatic sampling device for a bioreactor comprises a control cabinet, at least one set of sampling pipeline components for connecting to a sampling container, and at least one set of clamp valves for controlling the on-off of the sampling pipeline components. The at least one set of clamp valves is electrically connected to a controller provided inside the control cabinet.

[0006] Furthermore, the at least one set of sampling pipeline components is detachably arranged on the control cabinet, and the at least one set of pipe clamp valves is fixedly arranged on the control cabinet.

[0007] Furthermore, it also includes at least one peristaltic pump for controlling the flow of liquid in the sampling pipeline assembly. The at least one peristaltic pump is fixedly arranged on the control cabinet and electrically connected to the controller arranged inside the control cabinet.

[0008] Furthermore, it also includes a moving trolley, and the moving trolley is arranged at the bottom of the control cabinet.

[0009] Furthermore, the control cabinet is also provided with a touch screen, an indicator light and a power switch, and the touch screen, the indicator light and the power switch are all electrically connected to the controller provided inside the control cabinet.

[0010] Furthermore, the sampling pipeline assembly includes a main pipeline, at least one branch pipeline and at least one sampling tube, the main pipeline is used to connect the sampling container, and the branch pipeline is used to connect the main pipeline and the sampling tube.

[0011] Furthermore, one end of the main line is connected to a Luer connector and the other end is connected to a sterile plug. One end of each branch line is connected to the main line and the other end is connected to a sampling tube. Each branch line is also correspondingly clamped in a tube clamp valve.

[0012] Furthermore, each branch pipe is provided with a flow meter, and each flow meter is electrically connected to a controller provided inside the control cabinet.

[0013] Furthermore, each of the sampling tubes includes a sampling tube body and a sampling tube cover. The sampling tube cover is screwed onto the sampling tube body, and an air filter is also connected to the sampling tube cover.

[0014] Furthermore, a refrigeration component is provided inside the control cabinet, and the refrigeration component is used to cool the sampling tube.

[0015] Compared with the prior art, the advantages of the present invention are as follows:

[0016] (1) Good compatibility, can be connected to any bioreactor, only requires the bioreactor to provide a pipeline with a Luer interface connected to the liquid surface;

[0017] (2) Easy to operate, the entire sampling process is automatic, and you only need to set the corresponding sampling time, sampling quantity, sampling capacity and other parameters in advance;

[0018] (3) High sampling efficiency, multiple samples can be sampled at the same time;

[0019] (4) No need to clean or get contaminated, the entire sampling pipeline assembly is disposable and sterile. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solution in this embodiment, the following is a brief introduction to the drawings required for the description of the embodiment. Obviously, the drawings described below are only some embodiments of the present utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0021] Figure 1 This is an embodiment of the fully automatic sampling equipment for bioreactors of the utility model;

[0022] Figure 2 yes Figure 1 Left view in the embodiment;

[0023] Figure 3 yes Figure 1 A schematic diagram of the axonometric structure of the sampling pipeline assembly in the embodiment;

[0024] Figure 4 yes Figure 1 A left side view of the sampling pipeline assembly in the embodiment;

[0025] Figure 5 This is another specific embodiment of the fully automatic sampling device for bioreactors of the utility model;

[0026] Explanation of the accompanying symbols: 1. Control cabinet; 2. Sampling pipeline assembly; 3. Tube clamp valve; 4. Peristaltic pump; 5. Mobile trolley; 6. Touch screen; 7. Indicator light; 8. Power switch. DETAILED DESCRIPTION

[0027] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the following further describes how the present invention is implemented in conjunction with the accompanying drawings and specific implementation methods.

[0028] See Figures 1 to 4 The figure shows an embodiment of the fully automatic sampling device for bioreactors of the present invention. The device comprises a control cabinet 1, two sets of sampling line assemblies 2 for connecting to sampling containers, two sets of clamp valves 3 for controlling the on / off state of the sampling line assemblies 2, and two peristaltic pumps 4 for controlling the flow of liquid in the sampling line assemblies 2. The two peristaltic pumps 4 and the two sets of clamp valves 3 are electrically connected to a controller within the control cabinet 1. During operation, the two peristaltic pumps 4 and the two sets of clamp valves 3 perform corresponding actions under the control of the controller.

[0029] It can be understood that the number of sampling pipeline assemblies 2 is not limited to one set and can be increased or decreased according to actual needs; similarly, the number of tube clamp valves 3 is not limited to one group and can be increased or decreased according to actual needs; the number of peristaltic pumps 4 is not limited to one and can be increased or decreased according to actual needs.

[0030] Specifically, in this embodiment, the two sets of sampling pipeline assemblies 2 can be detachably arranged on the front panel of the control cabinet 1; the two sets of tube clamp valves 3 are fixedly arranged on the front panel of the control cabinet 1; the two peristaltic pumps 4 are respectively fixedly arranged on the left and right side panels of the control cabinet 1; wherein, each set of sampling pipeline assemblies 2 is correspondingly connected to a set of tube clamp valves 3 and a peristaltic pump 4.

[0031] Specifically, in this embodiment, the two sampling line assemblies 2 are connected to the same sampling container for sampling the same sampling container. In other embodiments, the two sampling line assemblies 2 are each connected to a sampling container for sampling the two sampling containers simultaneously.

[0032] Specifically, in this embodiment, each peristaltic pump 4 is mounted on the tubing (i.e., sterile tubing) connecting the corresponding sampling tubing assembly 2 to the sampling container. The peristaltic pump 4 ensures accurate sampling. When the peristaltic pump 4 moves in the forward direction, the sampled liquid is drawn from the sampling container and into the sampling tubing assembly 2. Once the desired sampling volume is reached, the remaining liquid in the sampling tubing assembly 2 is reversely drawn back into the sampling container by the peristaltic pump 4.

[0033] Specifically, in this embodiment, the sampling line assembly 2 is a disposable, sterile product that can be removed from the front panel of the control cabinet 1. This design reduces the risk of sample contamination. After each sampling, the sampling line assembly 2 can be removed and replaced with a new one for the next sampling.

[0034] Specifically, in this embodiment, a touch screen 6, an indicator light 7, and a power switch 8 are embedded on the front panel of the control cabinet 1. These touch screen 6, indicator light 7, and power switch 8 are all electrically connected to a controller within the control cabinet 1. The power switch 7 is used to control the power supply to the entire sampling device; the indicator light 7 indicates the operating status of the sampling device; and the touch screen 8 facilitates human-computer interaction with the operator, allowing the operator to set control parameters based on actual work needs so that the sampling device can automatically perform the corresponding sampling operation according to the set parameters. During use, the device only needs to press the power switch 8 on the control cabinet 1 and then set the corresponding parameters on the touch screen 6 to enable the device to perform automatic sampling operations.

[0035] Specifically, in this embodiment, each set of sampling pipeline assembly 2 includes a main pipeline 201 , 10 branch pipelines 202 and 10 sampling tubes 203 , wherein the main pipeline 201 is used to connect the sampling container, and the 10 branch pipelines 202 are used to connect the main pipeline 201 and the 10 sampling tubes 203 .

[0036] It is understandable that the number of branch pipes 202 and sampling tubes 203 included in each set of sampling pipe assembly 2 can be increased or decreased according to actual needs. In other words, each set of sampling pipe assembly 2 can only sample one sample at a time, or can sample multiple samples at a time.

[0037] Specifically, in this embodiment, each group of pinch valves 3 includes 10 pinch valves 3 , and each pinch valve 3 is connected to a corresponding branch pipeline 202 .

[0038] It is understood that the number of pinch valves 3 included in each set of pinch valves 3 matches the number of branch lines 202 included in each set of sampling line assembly 2. That is, one branch line 202 is connected to one pinch valve 3, and one pinch valve 3 controls the on / off function of one branch line 202. The number of pinch valves 3 can be increased or decreased based on actual needs.

[0039] Specifically, in this embodiment, one end of the main line 201 is connected to a Luer connector 204, and the other end is connected to a sterile plug 205, wherein the Luer connector 204 facilitates the connection of the main line 201 with the pipeline with a Luer interface (sterile pipeline) led out from the sampling container.

[0040] Specifically, in this embodiment, one end of each branch line 202 in each set of sampling line assembly 2 is connected to the corresponding main line 201, and the other end is connected to a corresponding sampling tube 203, and each branch line 202 is also clamped in a corresponding pipe clamp valve 3.

[0041] Specifically, in this embodiment, each sampling tube 203 includes a sampling tube body 203a and a sampling tube cover 203b. The sampling tube cover 203b is screwed onto the sampling tube body 203a. The sampling tube cover 203b is also connected to an air filter 203c. The capacity of the sampling tube body 203a is 5-30mL.

[0042] Specifically, in this embodiment, in order to accurately control the sample volume collected by each sampling tube 203, a flow meter (not shown) can be installed on each branch pipe 202 to monitor the flow rate entering the sampling tube 202 in real time. Of course, if the sampling accuracy requirement is not high, a flow meter can also be installed.

[0043] Specifically, the flowmeter can be an electromagnetic flowmeter, an ultrasonic flowmeter, or an optical flowmeter, and is electrically connected to a controller disposed within the control cabinet 1. During operation, when the flowmeter detects that the liquid entering the sampling tube 203 has reached the sampling volume, it first sends a corresponding detection signal to the controller, which then controls the corresponding clamp valve 3 to clamp the corresponding branch line 202, thereby cutting off the corresponding branch line 202 and stopping the infusion.

[0044] See Figure 5 FIG2 shows another embodiment of the fully automatic sampling device for bioreactors of the present invention, which differs from the first embodiment in that it further comprises a mobile trolley 5, and the mobile trolley 5 is arranged at the bottom of the control cabinet 1. The purpose of providing the mobile trolley 5 is to facilitate the movement of the fully automatic sampling device for bioreactors.

[0045] As another embodiment of the fully automatic sampling device for a bioreactor of the present invention, in order to meet the special preservation requirements for the sampled samples (i.e., refrigeration treatment), the fully automatic sampling device for a bioreactor of the present invention further includes a refrigeration component (not shown in the figure), and the refrigeration component can be a refrigerator or a semiconductor refrigeration plate, which is arranged inside the control cabinet 1 and electrically connected to the controller inside the control cabinet 1; the function of the refrigeration component is to cool the sampling tube 203 to prevent the sample from further reacting in the sampling tube 203 due to the high temperature.

[0046] The working principle of the utility model bioreactor automatic sampling equipment is as follows:

[0047] (1) Connect the sampling pipeline assembly 2 to the sampling container (i.e., bioreactor) where sampling is required;

[0048] (2) Install the sampling pipe assembly 2 on the front panel of the control cabinet 1;

[0049] (3) Turn on the power switch 8 on the front panel of the control cabinet 1, and set the corresponding sampling time, sampling volume and sampling number on the touch screen 6;

[0050] (4) Click the start sampling button on the touch screen 6 to start automatic sampling;

[0051] In the sampling process, before the required sample volume is reached, the peristaltic pump 4 rotates forward; when the required sample volume is reached, the peristaltic pump 4 rotates reversely;

[0052] When the peristaltic pump 4 rotates forward, the flow path of the sample solution is: sampling container → sterile pipeline → peristaltic pump 4 → sterile pipeline → main pipeline 201 → branch pipeline 202 → tube clamp valve 3 → branch pipeline 202 → sampling tube 203.

[0053] When the peristaltic pump 4 is in reverse operation, the flow path of the sample solution is: sampling tube 203 → branch line 202 → clamp valve 3 → branch line 202 → main line 201 → sterile line → peristaltic pump → sterile line → sampling container;

[0054] The sterile pipeline is a pipeline with a Luer interface leading out from the sampling container.

[0055] (5) After sampling is completed, disconnect the sampling pipeline assembly 2.

[0056] It should be noted that the controller, the pipe clamp valve 3, the peristaltic pump 4 and the flow meter adopted in the present invention are all existing technologies.

[0057] Finally, it should be noted that the above description is only an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the description and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A fully automatic sampling device for a bioreactor, characterized by: The invention comprises a control cabinet (1), at least one set of sampling pipeline components (2) for connecting to a sampling container, and at least one set of pipe clamp valves (3) for controlling the on-off of the sampling pipeline components (2), wherein the at least one set of pipe clamp valves (3) is electrically connected to a controller provided inside the control cabinet (1).

2. The fully automatic sampling device for bioreactors according to claim 1, characterized in that: The at least one set of sampling pipeline components (2) is detachably arranged on the control cabinet (1), and the at least one set of pipe clamp valves (3) is fixedly arranged on the control cabinet (1).

3. The fully automatic sampling device for bioreactors according to claim 1, characterized in that: It also includes at least one peristaltic pump (4) for controlling the flow of liquid in the sampling pipeline assembly (2), wherein the at least one peristaltic pump (4) is fixedly arranged on the control cabinet (1) and electrically connected to a controller arranged inside the control cabinet (1).

4. The fully automatic sampling device for bioreactors according to any one of claims 1 to 3, characterized in that: It also includes a moving trolley (5), and the moving trolley (5) is arranged at the bottom of the control cabinet (1).

5. The fully automatic sampling device for bioreactors according to claim 4, characterized in that: The control cabinet (1) is also provided with a touch screen (6), an indicator light (7) and a power switch (8), and the touch screen (6), the indicator light (7) and the power switch (8) are all electrically connected to a controller provided inside the control cabinet (1).

6. The fully automatic sampling device for bioreactors according to claim 1, characterized in that: The sampling pipeline assembly (2) comprises a main pipeline (201), at least one branch pipeline (202) and at least one sampling tube (203); the main pipeline (201) is used to connect to a sampling container, and the branch pipeline (202) is used to connect the main pipeline (201) and the sampling tube (203).

7. The fully automatic sampling device for bioreactors according to claim 6, characterized in that: One end of the main pipeline (201) is connected to a Luer connector (204), and the other end is connected to a sterile plug (205). One end of each branch pipeline (202) is connected to the main pipeline (201), and the other end is connected to a sampling tube (203). Each branch pipeline (202) is also correspondingly clamped in a tube clamp valve (3).

8. The fully automatic sampling device for bioreactors according to claim 7, characterized in that: Each branch pipe (202) is also provided with a flow meter, and each flow meter is electrically connected to a controller provided inside the control cabinet (1).

9. The fully automatic sampling device for bioreactors according to claim 7, characterized in that: Each sampling tube (203) comprises a sampling tube body (203a) and a sampling tube cover (203b), wherein the sampling tube cover (203b) is screwed onto the sampling tube body (203a), and an air filter (203c) is also connected to the sampling tube cover (203b).

10. The fully automatic sampling device for bioreactors according to claim 8, characterized in that: A refrigeration component is also provided inside the control cabinet (1), and the refrigeration component is used to cool the sampling tube (203).