Adenovirus vector purification device

Abstract

The utility model relates to the technical field of carrier purification, in particular to an adenovirus carrier purification device, which is provided with a pressurizing structure capable of being opened and closed, meets the requirement of rapid purification, is convenient to assemble and disassemble an internal filter membrane, and improves the practicability. Comprising a base cylinder, a discharge head, a one-way valve, clamping grooves, a sealing ring gasket, a purification filter membrane, a hinge, a movable sliding cylinder, a piston, a connecting rod, a handle piece, a spiral groove frame, a fixing piece, a fixing bolt and a clamping block, the bottom end of the base cylinder is communicated with the top end of the discharge head, the one-way valve is arranged on the discharge head, multiple sets of clamping grooves are formed in the inner side of the base cylinder, and the outer side of the purification filter membrane is connected with the inner side of the sealing ring gasket; the middle of the left side of the top end of the base cylinder is movably connected with the middle of the right side of the top end of the movable sliding cylinder through a hinge, the inner side of the movable sliding cylinder is slidably connected with the outer side of the piston, and the middle of the bottom end of the piston is connected with the top end of the handle piece through a connecting rod. The middle-upper side of the right end of the base cylinder is connected with the inner end of the spiral groove frame.

Description

Technical Field

[0001] The utility model relates to the technical field of vector purification, in particular to an adenovirus vector purification device. Background Art

[0002] Viral vectors can carry genetic material into cells by exploiting the molecular mechanisms of viruses to transmit their genomes into other cells for infection. This can occur in intact living organisms (in vivo) or in cell culture (in vitro) and can be applied to basic research, gene therapy, or vaccines.

[0003] The adenoviral vector purification device is used to purify adenoviral vectors. Modern viral research often requires large quantities of pure viral particles for chemical research or antibody preparation. Common purification methods include ultrafiltration, adsorption, and chromatography. Ultrafiltration uses an ultrafiltration membrane to remove water, salts, and small molecules, while retaining large molecules or particles such as viruses, thereby achieving purification.

[0004] However, most of these devices have the disadvantage of being difficult to replace the ultrafiltration membrane, making them less practical. Utility Model Content

[0005] (1) Technical problems solved

[0006] In response to the deficiencies of the prior art, the present invention provides an adenovirus vector purification device that is equipped with an openable and closable pressurized structure, which not only meets the requirements of rapid purification but also facilitates the loading and unloading of the internal filter membrane, thereby improving practicality.

[0007] (2) Technical solution

[0008] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an adenovirus vector purification device, comprising a base cylinder, a discharge head, a one-way valve, a card groove, a sealing ring gasket, a purification filter membrane, a hinge, a movable slide, a piston, a connecting rod, a handle, a screw groove frame, a fixed plate, a fixing bolt and a card block, the bottom end of the base cylinder is connected to the top end of the discharge head, the discharge head is provided with a one-way valve, the inner side of the base cylinder is provided with multiple groups of card grooves, the outer side of the purification filter membrane is connected to the inner side of the sealing ring gasket, the outer side of the sealing ring gasket is provided with multiple groups of card blocks, the middle left side of the top end of the base cylinder is movably connected to the middle right side of the top end of the movable slide via a hinge, the inner side of the movable slide is slidably connected to the outer side of the piston, the middle of the bottom end of the piston is connected to the top end of the handle via a connecting rod, the middle upper side of the left end of the movable slide is connected to the inner end of the fixed plate, the inner side of the fixed plate is movably provided with a fixing bolt, and the middle upper side of the right end of the base cylinder is connected to the inner end of the screw groove frame.

[0009] Preferably, a second sealing ring gasket is further included, and the top ring side of the movable slide is connected to the bottom end of the second sealing ring gasket.

[0010] Preferably, a limiting frame is further included, and the upper and lower end ring sides inside the movable slide are respectively connected to the outer sides of multiple groups of limiting frames.

[0011] Preferably, it further comprises a second handle, and the top end of each group of the card blocks is respectively connected to the bottom end of a group of second handles.

[0012] Preferably, it further comprises a bracket and a base, the lower side of the outer end of the base tube is connected to the inner ends of multiple groups of brackets, and the bottom ends of all the brackets are connected to the outer side of the top end of the base.

[0013] Preferably, an anti-knock pad is further included, and the middle part of the left end of the base tube is connected to the inner side of the anti-knock pad.

[0014] (3) Beneficial effects

[0015] Compared with the prior art, the present invention provides an adenovirus vector purification device with the following features:

[0016] Beneficial effects:

[0017] The purification filter membrane is installed on the inner side of the base cylinder, which is clamped in the corresponding clamping groove by the clamping block and sealed on the inner wall of the base cylinder by the sealing ring. When in use, the carrier liquid is first poured into the inside of the base cylinder, and then the movable slide is closed under the constraint of the hinge, so that the screw groove frame and the fixed plate correspond, and the fixing bolt is screwed into the screw groove frame through the fixed plate, and then the plate is pressed to drive the piston downward through the connecting rod to pressurize the inside of the base cylinder, and the carrier liquid is purified by the purification filter membrane. Under the conduction action of the one-way valve, it is discharged downward through the discharge head. A pressurized structure that can be opened and closed is provided to meet the requirements of rapid purification while facilitating the loading and unloading of the internal filter membrane and improving practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is an axial view of the schematic structural diagram of the utility model;

[0019] Figure 2 For this utility model Figure 1 Front view of

[0020] Figure 3 For this utility model Figure 1 Bottom view of

[0021] Figure 4 For this utility model Figure 1 Left view of .

[0022] Markings in the accompanying drawings: 1. Base cylinder; 2. Discharge head; 3. One-way valve; 4. Card groove; 5. Sealing ring gasket; 6. Purification filter membrane; 7. Hinge; 8. Moving slide; 9. Piston; 10. Connecting rod; 11. Handle; 12. Screw groove frame; 13. Fixed plate; 14. Fixing bolt; 15. Second sealing ring gasket; 16. Limit frame; 17. Second handle; 18. Bracket; 19. Base; 20. Anti-knock pad; 21. Card block. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] Example

[0025] See also Figure 1-4 , an adenovirus vector purification device, including a base cylinder 1, a discharge head 2, a one-way valve 3, a card groove 4, a sealing ring gasket 5, a purification filter membrane 6, a hinge 7, a movable slide 8, a piston 9, a connecting rod 10, a handle 11, a screw groove frame 12, a fixing plate 13, a fixing bolt 14 and a block 21. The bottom end of the base cylinder 1 is connected to the top of the discharge head 2, and the discharge head 2 is provided with a one-way valve 3. The inner side of the base cylinder 1 is provided with multiple groups of card grooves 4. The outer side of the purification filter membrane 6 is connected to the inner side of the sealing ring gasket 5. The outer side of the sealing ring gasket 5 is provided with multiple groups of blocks 21. The middle part of the left side of the top of the base cylinder 1 is movably connected to the middle part of the right side of the top of the movable slide 8 through the hinge 7. The inner side of the movable slide 8 is slidably connected to the outer side of the piston 9. The middle part of the bottom end of the piston 9 is connected to the top of the handle 11 through the connecting rod 10. The upper middle side of the left end of the cylinder 8 is connected to the inner end of the fixed plate 13, and a fixing bolt 14 is movably provided on the inner side of the fixed plate 13. The upper middle side of the right end of the base cylinder 1 is connected to the inner end of the screw groove frame 12; the purification filter membrane 6 is installed on the inner side of the base cylinder 1, and is clamped in the corresponding clamping groove 4 by the clamping block 21, and is sealed on the inner wall of the base cylinder 1 by the sealing ring gasket 5. When in use, the carrier liquid is first poured into the interior of the base cylinder 1, and then the movable slide cylinder 8 is closed under the constraint of the hinge 7 so that the screw groove frame 12 and the fixed plate 13 correspond, and the fixing bolt 14 is screwed into the screw groove frame 12 through the fixed plate 13, and then the handle 11 is pressed, and the piston 9 is driven downward by the connecting rod 10 to pressurize the interior of the base cylinder 1, and the carrier liquid is purified by the purification filter membrane 6. Under the conduction action of the one-way valve 3, it is discharged downward through the discharge head 2.

[0026] It also includes a sealing ring gasket 2 15, and the top ring side of the movable slide 8 is connected to the bottom end of the sealing ring gasket 2 15; through the sealing ring gasket 2 15, it can be in contact and sealed with the top of the base tube 1 after the movable slide 8 is closed, avoiding leakage and improving reliability.

[0027] It also includes a limit frame 16. The upper and lower end ring sides inside the movable slide 8 are respectively connected to the outer sides of multiple groups of limit frames 16. The limit frames 16 on the same horizontal side can limit the position of the piston 9 to avoid sliding out and improve reliability.

[0028] It also includes a handle piece 17, and the top of each group of card blocks 21 is connected to the bottom of a group of handle pieces 17; by pinching the handle piece 17, the card blocks 21 can move in the card slot 4, and then move to the sealing ring gasket 5 and the purification filter membrane 6, which is convenient for loading and unloading.

[0029] It also includes a bracket 18 and a base 19. The lower side of the outer end of the base tube 1 is connected to the inner ends of multiple groups of brackets 18, and the bottom ends of all brackets 18 are connected to the outer side of the top end of the base 19; the bracket 18 can support the base tube 1 and the corresponding overall device, and is contacted by the bottom end of the base 19 to create space, facilitate liquid collection, and improve stability.

[0030] It also includes an anti-knock pad 20, and the middle part of the left end of the base cylinder 1 is connected to the inner side of the anti-knock pad 20; the movable slide cylinder 8 can be contacted after opening through the anti-knock pad 20 to avoid collision and damage, thereby improving reliability.

[0031] In summary, when an adenovirus vector purification device is used, the purification filter membrane 6 is installed on the inner side of the base cylinder 1, the pinching piece 2 17 is clamped in the corresponding card groove 4 by the clamping block 21, and the sealing ring gasket 5 is sealed on the inner wall of the base cylinder 1. When in use, the base cylinder 1 and the corresponding overall device can be supported by the bracket 18, and the bottom end of the base 19 is in contact to create a space. The carrier liquid is first poured into the inside of the base cylinder 1, and then the movable slide 8 is closed under the constraint of the hinge 7. After the movable slide 8 is closed, it can be connected to the top of the base cylinder 1 through the sealing ring gasket 2 15. The end contact seal is used to make the screw groove frame 12 and the fixed plate 13 correspond, and the fixing bolt 14 is screwed into the screw groove frame 12 through the fixed plate 13, and then the handle 11 is pressed to drive the piston 9 downward through the connecting rod 10 to pressurize the inside of the base cylinder 1, and the carrier liquid is purified by the purification filter membrane 6. Under the conduction action of the one-way valve 3, it is discharged downward through the discharge head 2. The piston 9 can be limited to this position by the limit frame 16 on the same horizontal side to prevent it from slipping out. In addition, the contact after the movable slide 8 is opened can be carried out through the anti-knock pad 20 to avoid collision and damage.

[0032] This one-way valve 3 and purification filter membrane 6 are well-known devices purchased directly from the market by those skilled in the art. Here we only use them without making any structural or functional improvements to them, and we will not go into details here.

[0033] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An adenovirus vector purification device, characterized in that: The invention comprises a base cylinder (1), a discharge head (2), a one-way valve (3), a card slot (4), a sealing ring gasket (5), a purification filter membrane (6), a hinge (7), a movable slide cylinder (8), a piston (9), a connecting rod (10), a handle (11), a screw groove frame (12), a fixing plate (13), a fixing bolt (14) and a card block (21). The bottom end of the base cylinder (1) is connected to the top end of the discharge head (2), the discharge head (2) is provided with a one-way valve (3), the inner side of the base cylinder (1) is provided with multiple groups of card slots (4), the outer side of the purification filter membrane (6) and the sealing ring gasket (5) are connected. The inner sides are connected, and multiple groups of card blocks (21) are arranged on the outer side of the sealing ring gasket (5). The middle of the left side of the top of the base cylinder (1) is movably connected to the middle of the right side of the top of the movable slide (8) through the hinge (7). The inner side of the movable slide (8) is slidably connected to the outer side of the piston (9). The middle of the bottom end of the piston (9) is connected to the top of the handle (11) through the connecting rod (10). The upper middle side of the left end of the movable slide (8) is connected to the inner end of the fixed plate (13). The inner side of the fixed plate (13) is movably provided with a fixing bolt (14). The upper middle side of the right end of the base cylinder (1) is connected to the inner end of the screw groove frame (12).

2. The adenovirus vector purification device according to claim 1, characterized in that: It also includes a second sealing ring gasket (15), and the top ring side of the movable slide (8) is connected to the bottom end of the second sealing ring gasket (15).

3. The adenovirus vector purification device according to claim 1, characterized in that: It also includes a limiting frame (16), and the upper and lower end ring sides inside the movable slide (8) are respectively connected to the outer sides of multiple groups of limiting frames (16).

4. The adenovirus vector purification device according to claim 1, characterized in that: It also includes a second handle piece (17), and the top end of each group of the card blocks (21) is respectively connected to the bottom end of a group of second handle pieces (17).

5. The adenovirus vector purification device according to claim 1, characterized in that: It also includes a bracket (18) and a base (19), wherein the lower side of the outer end of the base tube (1) is connected to the inner ends of multiple groups of brackets (18), and the bottom ends of all brackets (18) are connected to the outer side of the top end of the base (19).

6. The adenovirus vector purification device according to claim 1, characterized in that: It also includes an anti-knock pad (20), and the middle part of the left end of the base tube (1) is connected to the inner side of the anti-knock pad (20).

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