Cell culture plate

Abstract

The utility model discloses a cell culture plate, and relates to the technical field of cell culture plates, the cell culture plate comprises a bottom plate, the upper part of the bottom plate is provided with a well-sealed upper cover plate, the inner cavity of the upper part of the bottom plate is fixedly provided with latticed partition plate strips, the partition plate strips are internally and fixedly provided with a plurality of reagent grooves arranged in an array, and the peripheries of the reagent grooves are fixedly provided with outer plate strips fixedly connected with the partition plate strips; a plurality of vertical plates capable of forming a liquid injection bin are fixedly arranged on the periphery of the outer batten, symmetrical inserting grooves are formed in the four corners of the upper cover plate in a penetrating mode, four lock catch mechanisms identical in structure and installation mode are arranged on the lower portions of the four inserting grooves, and vertical moving mechanisms are arranged on the upper portions of the inserting grooves. According to the invention, the fringe effect of the culture plates is improved, and the problem that the culture plates easily topple during stacking is solved.

Description

Technical Field

[0001] The present application relates to the field of cell culture plate technology, and in particular to a cell culture plate. Background Art

[0002] Cell culture plates have good transparency, are non-toxic, and sterile, and can promote the growth and reproduction of primary cells such as nerve cells, passaged cells such as tumor cells, and other animal and human cells. Currently, commonly used cell culture plates are one-piece porous structures with multiple fixed unit wells. During cell culture, the culture personnel place a cell culture plate with multiple unit wells in the same cell culture box to facilitate the growth and reproduction of the same type of cells in a unified and balanced environment.

[0003] For example, the cell culture plate with the application number CN201620220909.2 that can prevent edge effects has the following deficiencies:

[0004] The above patent sets a new circle of grooves on the edge for adding sterile liquids such as PBS or distilled water to "protect" the internal experimental wells and prevent the evaporation of liquid in the cell culture wells at the edge. However, the improvement effect of this setting is very small and cannot achieve a balance between the edge temperature and the center temperature. In actual use, in order to save space, two culture plates are stacked and stored. However, due to the lack of fixing measures, the stacked culture plates may cause them to fall over when shaken or when the number of stacked layers is high. Utility Model Content

[0005] In order to improve the edge effect of culture plates and the problem of tipping over easily occurring during stacking, the present application provides a cell culture plate.

[0006] The cell culture plate provided in this application adopts the following technical solution:

[0007] A cell culture plate, comprising a base plate, a well-sealed upper cover plate provided on the upper portion of the base plate, a grid-shaped partition strip fixed in an inner cavity of the upper portion of the base plate, a plurality of reagent tanks arranged in an array fixed in the partition strip, an outer strip fixed to the outer periphery of the reagent tanks fixed to the partition strip, and a plurality of vertical plates that can form a liquid injection chamber fixed around the outer strip;

[0008] The four corners of the upper cover are provided with mutually symmetrical slots, and the lower parts of the four slots are provided with four locking mechanisms with the same structure and installation method. The locking mechanism includes an inserting plate fixed to the lower surface of the inner cavity of the slot, a hollow protective platform is provided on the upper part of the inserting plate, and a first latch is slidably provided in the middle of the bottom surface of the protective platform. A rack A is fixed on the upper part of the first latch, and a first gear is meshed with the upper part of the rack A;

[0009] A vertical moving mechanism is provided on the upper part of the slot, and the vertical moving mechanism includes a rotating shaft fixed through the middle of the first gear, a second gear is fixed on one side of the rotating shaft, a rack C is meshed with one side of the second gear, a fixed push plate is fixed on the side of the rack C away from the second gear, a horizontal limiting groove is provided through one side of the fixed push plate, and a circular push rod that is movable and passes through the side wall of the upper cover plate is slidably provided inside the horizontal limiting groove.

[0010] By adopting the above technical solution, the partition strips and the outer strips cooperate to arrange the reagent tank in zones to achieve zoned temperature control, and the vertical plates can form a circle of temperature control units on the outer circle of the reagent tank, and the plug plates can help to guide and fix the two stacked devices, and the bottom plate and the upper cover plate are clamped and fixed through the cooperation of the first pin, rack A and the first gear, and the circular push rod and rack C in the vertical moving mechanism cooperate to drive the rotating shaft and the second gear to rotate to transmit power to the first gear.

[0011] Preferably, the locking mechanism also includes a first T-shaped groove opened in the side wall of one side of the slot, a second pin is slidably arranged inside the first T-shaped groove, a rack B is fixed to the lower part of the second pin, and the rack B is arranged to be meshed with the first gear at the lower part away from the second pin.

[0012] By adopting the above technical solution, the rack B cooperates with the second latch to limit the position of the two stacked devices without affecting the clamping and fixing of the devices themselves.

[0013] Preferably, a second T-shaped slot which can be engaged with the second latch is provided at the lower portion of the plug board.

[0014] By adopting the above technical solution, the second T-shaped slide groove can be provided to clamp and fix the two devices when they are stacked to prevent them from loosening.

[0015] Preferably, the vertical movement mechanism further comprises a vertical movement groove penetratingly provided on a side wall of one side of the slot, and the inner surface of the vertical movement groove is slidably provided with an end of the circular push rod away from the fixed push plate.

[0016] By adopting the above technical solution, the vertical movable groove can limit and guide the circular push rod.

[0017] Preferably, the vertical moving groove is longitudinally spaced apart with three transverse limiting grooves of the same structure and slidably connected to the circular push rod, and the inner cavity of the transverse limiting groove is symmetrically fixed with two U-shaped limiting blocks that can be engaged with the circular push rod.

[0018] By adopting the above technical solution, the transverse limiting groove and the U-shaped limiting block cooperate with each other to clamp and limit the circular push rod.

[0019] Preferably, a scale is fixed on the inner wall of the reagent tank to help accurately add culture medium.

[0020] By adopting the above technical solution, the scale can help staff to add culture medium more accurately.

[0021] Preferably, a sealing plate fixedly connected to the outer strips is fixedly provided on the upper portion of the plurality of vertical plates.

[0022] By adopting the above technical solution, the sealing plate can effectively seal the space separated by the vertical plate, the outer strips and the bottom plate, so that the injected sterile water will not have the risk of leakage when shaking.

[0023] Preferably, a heat pipe is fixedly provided inside the partition strip and is fixedly provided through the outer strip.

[0024] By adopting the above technical solution, the heat pipe and the outer strip are fixedly penetrated so that the heat pipe can be connected to the space isolated by the vertical plate, which helps to better conduct heat.

[0025] In summary, this application includes at least one of the following beneficial technical effects:

[0026] 1. The reagent tanks are placed in a separate space by using the partition strips and the outer strips. Heat pipes with good thermal conductivity are set in the partition strips for heat conduction. The sterile water-carrying space formed between the vertical plate and the sealing plate is used to separate the peripheral reagent tanks, thereby reducing the temperature unevenness between the edge reagent tanks and the center reagent tanks.

[0027] 2. With the help of the locking mechanism and the vertical movement mechanism, not only can the bottom plate and the upper cover be fixed together, but also two culture plates can be fixed in place when stacked to prevent tipping due to vibration or stacking too high. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the overall device of this application;

[0029] Figure 2 This is the internal structure diagram of the base plate of this application;

[0030] Figure 3 This is an enlarged view of the zoned temperature control structure of this application;

[0031] Figure 4 This is a diagram of the two mechanisms within the slot of this application;

[0032] Figure 5 This is a schematic diagram of the position of the vertical movable slot of this application;

[0033] Figure 6 This is a diagram showing the structure of the transverse limit groove of this application;

[0034] Figure 7 This is a schematic diagram of the two culture plates stacked and fixed in this application.

[0035] Reference numerals: 1, bottom plate; 2, upper cover plate; 3, partition bar; 4, reagent tank;

[0036] 5. Locking mechanism; 51. Insert plate; 52. Protective platform; 53. First latch; 54. Rack A; 55. First gear; 56. Rack B; 57. Second latch; 58. First T-shaped slide; 59. Second T-shaped slide;

[0037] 6. Vertical board; 7. Outer slats; 8. Slots;

[0038] 9. Vertical moving mechanism; 91. Rotating shaft; 92. Second gear; 93. Rack C; 94. Fixed push plate; 95. Circular push rod; 96. Vertical moving groove; 97. Horizontal limit groove; 98. U-shaped limit block;

[0039] 10. Scale; 11. Sealing plate; 12. Heat pipe. DETAILED DESCRIPTION

[0040] The following is combined with Figure 1 - Figure 7 This application is described in further detail.

[0041] The embodiments of the present application disclose a cell culture plate.

[0042] Example 1

[0043] Reference Figure 1 、 Figure 2 、 Figure 3A cell culture plate comprises a bottom plate 1 placed on a table, and the upper part of the bottom plate 1 is engaged with an upper cover plate 2 with good sealing performance, and a space larger than the size of the upper cover plate 2 is provided at the lower part of the bottom plate 1, and the bottom plate 1 can be stacked with the upper cover plate 2 by being engaged with the upper cover plate 2, and the middle part of the inner cavity of the upper part of the bottom plate 1 is fixedly connected with the partition bar 3, and the partition bar 3 is arranged in a grid shape and evenly arranged on the upper part of the bottom plate 1, and a plurality of heat pipes 12 distributed vertically and horizontally are fixedly arranged inside the partition bar 3, and both ends of the plurality of heat pipes 12 are fixedly passed through the outer plate bar 7 and extend to the outside, and a plurality of array-arranged test tubes are fixedly arranged in the grid-like space of the partition bar 3. The reagent tank 4 is fixed with a scale 10 on the inner wall of the reagent tank 4 to help accurately add the culture medium and control the filling of the culture medium. The reagent tank 4 is fixedly connected to the outer strips 7 on all sides, and the outer strips 7 are fixedly connected to the partition strips 3 to divide the reagent tank 4 into several separate areas. The outer strips 7 are fixedly connected to several vertical plates 6 with the same structure and installation method on all sides, and the several vertical plates 6 are spaced apart to form several connecting spaces that can form a liquid filling bin, and the upper surfaces of the several vertical plates 6 are fixedly connected to the lower surface of the sealing plate 11, and the sealing plate 11 is fixedly connected to the outer strips 7 and the side walls of the upper cover plate 2.

[0044] The lower part of the bottom plate 1 is provided with an open space that is larger than the overall size of the upper cover plate 2, so that the lower part of the bottom plate 1 can be snapped into the upper part of the upper cover plate 2, so that the entire device can be stacked and stored, and the partition strips 3 and the outer strips 7 cooperate with each other to separate several independent spaces. The reagent tank 4 is arranged inside to ensure that each reagent tank 4 is an independent individual and will not interfere with each other to cause inaccuracy in the experiment. The vertical plate 6 can divide the space between the upper cover plate 2 and the outer strip 7 into pieces of areas, and each area is interconnected, and is sealed with a sealing plate 11, so that each separate space can be filled with sterile water to prevent edge effects in cell culture, and a heat pipe 12 with good thermal conductivity is provided in the outer strip 7, and the heat pipe 12 passes through the outer strip 7 to connect with each cavity and is immersed in sterile water, which can achieve the effect of sharing heat to achieve temperature control.

[0045] Reference Figure 1 、 Figure 4, four slots 8 of the same structure are set through the four corners of the upper cover 2, and the four slots 8 are symmetrically arranged, and a locking mechanism 5 is set at the lower part of the four slots 8, and the structure and installation method of the four locking mechanisms 5 are the same, and the locking mechanism 5 includes a plug-in plate 51 fixedly connected to the lower surface of the inner cavity of the slot 8, and a second T-shaped slide groove 59 is opened in the middle of the lower surface of the plug-in plate 51, and the second T-shaped slide groove 59 and the second latch 57 are both set to smooth surfaces and can be engaged with each other, and a hollow protective platform 52 with an upward opening is set on the upper surface of the plug-in plate 51, and the middle part of the bottom surface of the protective platform 52 is slidably connected to the lower surface of the first latch 53, and the middle part of the upper surface of the first latch 53 is fixedly connected to the lower surface of the rack A54, and the protective platform 52 is located on one side of the second latch 57. An opening for the first latch 53 and the rack A54 to slide out is opened, and the upper part of the rack A54 is meshed with the first gear 55.

[0046] Reference Figure 4 , and a first T-shaped slide groove 58 is opened in the side wall of one side of the lower part of the inner cavity of the slot 8, and the inner surface of the first T-shaped slide groove 58 is set to a smooth surface and is slidably connected with the second pin 57 with a smooth surface, and the length of the second pin 57 is set to half the length of the first T-shaped slide groove 58, and the length of the first T-shaped slide groove 58 is consistent with the length of the first pin 53, and the middle part of the lower surface of the second pin 57 is fixedly connected to the upper surface of the rack B56, and the lower part of the rack B56 away from the second pin 57 is meshed with the first gear 55.

[0047] The length and width of the slot 8 are greater than the length and width of the inserting plate 51, so that the inserting plate 51 can be smoothly inserted into the slot 8, and the first gear 55 in the locking mechanism 5 is engaged with the rack A54 and the rack B56, so that when the first gear 55 rotates, it can drive the rack A54 and the rack B56 to move, and the rack A54 and the rack B56 are arranged opposite to each other and move in opposite directions, and when the rack A54 and the rack B56 move, they can drive the first latch 53 and the second latch 57 to move together. The second latch 57 is half the length of the first latch 53, so the stroke of the first latch 53 is twice that of the second latch 57. When the first latch 53 is pushed out halfway and the bottom plate 1 and the upper cover plate 2 are clamped together, the second latch 57 is located at the opening of the first T-shaped slide groove 58. When the plug plate 51 is inserted into the slot 8 and abuts against the protective platform 52, the second latch 57 is pushed out and clamped with the second T-shaped slide groove 59, so that the two devices will not fall over due to being stacked too high or due to vibration.

[0048] Reference Figure 4 、 Figure 5 、 Figure 6, and a vertical moving mechanism 9 is provided at the upper part of the inner cavity of the slot 8, and the vertical moving mechanism 9 includes a rotating shaft 91 fixedly set through the middle of the first gear 55, and the two ends of the rotating shaft 91 are respectively transferred to the middle of the side walls on both sides of the protective platform 52, and the outer surface of the rotating shaft 91 away from the first gear 55 is fixedly connected to the inner surface of the second gear 92, and the second gear 92 away from the second pin 57 is meshed with the rack C93, and the surface of the rack C93 away from the second gear 92 is fixedly connected to the lower surface of the fixed push plate 94, and the fixed push plate 94 is provided with a horizontal limiting groove 97 with a height greater than the diameter of the circular push rod 95, and the inner surface of the horizontal limiting groove 97 is slidably set with the outer surface of the circular push rod 95, and the circular push rod 95 is slidably set in the vertical moving groove 96 at one end away from the fixed push plate 94 and movably passes through the side wall of one side of the slot 8.

[0049] Figure 5 、 Figure 6 A vertical movable groove 96 is provided on the side wall of the slot 8 away from the first gear 55, and a circular push rod 95 is slidably provided on the inner surface of the vertical movable groove 96, and a transverse limit groove 97 is provided on the upper part of the vertical movable groove 96, and transverse limit grooves 97 are provided in the middle and lower parts, and the three transverse limit grooves 97 are the same as the transverse limit grooves 97 provided in the fixed push plate 94, and the three transverse limit grooves 97 with the same structure are all connected to the inner cavity of the vertical movable groove 96, and can be slidably connected with the circular push rod 95, and U-shaped limit blocks 98 are symmetrically fixed on the upper and lower surfaces of the inner cavity of the four transverse limit grooves 97, and the two U-shaped limit blocks 98 can be engaged with the circular push rod 95 for limiting.

[0050] The vertical movement groove 96 is provided on the side wall of the slot 8 and is slidably connected to the circular push rod 95, so that the circular push rod 95 can drive the fixed push plate 94 and the rack C93 to move longitudinally, and drive the second gear 92 and the rotating shaft 91 to rotate, thereby driving the first gear 55 to rotate. The horizontal limit grooves 97 provided at the upper, middle and lower parts of the vertical movement groove 96 and the U-shaped limit blocks 98 matched with the horizontal limit grooves 97 can limit the circular push rod 95 at different stages, so that the bottom plate 1 and the upper cover are in a straight line. When plate 2 is connected alone, it is only necessary to push the circular push rod 95 into the horizontal limit groove 97 in the middle of the vertical moving groove 96 to push out the rack A54 in the locking mechanism 5 for limiting. When the two devices are stacked, the circular push rod 95 is pushed from the horizontal limit groove 97 in the middle of the vertical moving groove 96 to the horizontal limit groove 97 at the bottom of the circular push rod 95 for limiting. At this time, the second pin 57 in the locking mechanism 5 is pushed out to engage with the second T-shaped slide groove 59 in the upper device for limiting and fixing.

[0051] The implementation principle of a cell culture plate in the embodiment of the present application is as follows: after the staff injects appropriate cell samples and culture medium into the reagent tank 4, the upper cover plate 2 is covered with the bottom plate 1, and the insert plates 51 set at the four corners of the upper cover plate 2 are aligned with the holes of the slots 8 and inserted. After the bottom plate 1 and the upper cover plate 2 are docked, the circular push rod 95 is pushed so that the circular push rod 95 is pushed from the horizontal limit groove 97 at the upper part of the vertical moving groove 96 to the horizontal limit groove 97 in the middle of the vertical moving groove 96, and is inserted into the horizontal limit groove 97 through the insert plates 51 set at the four corners of the upper cover plate 2. The U-shaped limit block 98 is used for limiting, and during the movement, the circular push rod 95 pushes the fixed push plate 94 downward, and drives the rack C93 to move downward together, thereby driving the second gear 92 and the rotating shaft 91 to rotate. When the rotating shaft 91 rotates, it can drive the first gear 55 to rotate, thereby driving the rack A54 and the second pin 57 to move toward the middle, and the rack A54 will be pushed out to a general length for limiting the bottom plate 1 and the upper cover plate 2, and the second pin 57 moves to the opening of the first T-shaped slide groove 58.

[0052] When the staff needs to stack two devices for storage, the circular push rod 95 can be moved from the horizontal limit groove 97 in the middle of the vertical moving groove 96 to the horizontal limit groove 97 at the lower part of the vertical moving groove 96 for limiting. At this time, the second pin 57 located at the opening of the first T-shaped slide groove 58 will be completely pushed out to clamp and fix the two devices, and the rack A54 will also be pushed out to prevent tipping due to vibration and stacking too high.

[0053] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A cell culture plate, characterized in that: The invention comprises a bottom plate (1), a well-sealed upper cover plate (2) is provided on the upper part of the bottom plate (1), a grid-shaped partition strip (3) is fixed in the inner cavity of the upper part of the bottom plate (1), a plurality of arrayed reagent tanks (4) are fixed in the partition strip (3), an outer plate strip (7) fixed to the partition strip (3) is fixed on the periphery of the reagent tank (4), and a plurality of vertical plates (6) that can form a liquid injection bin are fixed around the outer plate strip (7); The four corners of the upper cover (2) are penetrated by mutually symmetrical slots (8), and the lower parts of the four slots (8) are provided with four locking mechanisms (5) with the same structure and installation method, and the locking mechanism (5) includes an inserting plate (51) fixed on the lower surface of the inner cavity of the slot (8), a hollow protective platform (52) is provided on the upper part of the inserting plate (51), and a first latch (53) is slidably provided on the middle part of the bottom surface of the protective platform (52), a rack A (54) is fixed on the upper part of the first latch (53), and a first gear (55) is meshed with the upper part of the rack A (54); A vertical moving mechanism (9) is provided on the upper portion of the slot (8), and the vertical moving mechanism (9) includes a rotating shaft (91) fixedly passing through the middle portion of the first gear (55), a second gear (92) fixedly provided on one side of the rotating shaft (91), a rack C (93) meshingly provided on one side of the second gear (92), a fixed push plate (94) fixedly provided on the side of the rack C (93) away from the second gear (92), a transverse limiting groove (97) passing through one side of the fixed push plate (94), and a circular push rod (95) movably passing through the side wall of the upper cover plate (2) slidably provided inside the transverse limiting groove (97).

2. A cell culture plate according to claim 1, characterized in that: The locking mechanism (5) further comprises a first T-shaped slot (58) provided in a side wall of one side of the slot (8), a second latch (57) being slidably provided inside the first T-shaped slot (58), a rack B (56) being fixedly provided at the lower portion of the second latch (57), and the rack B (56) being meshed with the first gear (55) at the lower portion of the side away from the second latch (57).

3. A cell culture plate according to claim 1, characterized in that: A second T-shaped sliding groove (59) capable of being engaged with a second latch (57) is provided at the lower portion of the inserting plate (51).

4. A cell culture plate according to claim 1, characterized in that: The vertical movement mechanism (9) further comprises a vertical movement groove (96) penetrating a side wall of the slot (8), wherein the inner surface of the vertical movement groove (96) and the circular push rod (95) are slidably arranged away from one end of the fixed push plate (94).

5. A cell culture plate according to claim 4, characterized in that: The vertical moving groove (96) is longitudinally spaced apart with three transverse limiting grooves (97) of the same structure and slidably connected to the circular push rod (95), and the inner cavity of the transverse limiting groove (97) is symmetrically fixed with two U-shaped limiting blocks (98) that can be clamped with the circular push rod (95).

6. A cell culture plate according to claim 1, characterized in that: The inner wall of the reagent tank (4) is provided with a scale (10) for facilitating accurate addition of culture fluid.

7. The cell culture plate according to claim 1, characterized in that: A sealing plate (11) fixedly connected to the outer strip (7) is fixedly provided on the upper part of the plurality of vertical plates (6).

8. The cell culture plate according to claim 1, wherein: A heat pipe (12) is fixedly provided inside the partition strip (3) and is fixedly arranged to penetrate the outer strip (7).

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