Gel electrophoresis nucleic acid recovery chip
By designing a gel electrophoresis nucleic acid recovery chip and adopting automated electrophoresis technology, the problem of complex operation of conventional devices is solved, the nucleic acid recovery is simplified and automated, the manual operation steps are reduced, and the simplicity and efficiency of the process are improved.
Patent Information
- Application Number
- CN202423131779.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-18
- Publication Date
- 2025-09-12
- Estimated Expiration
- 2034-12-18
AI Technical Summary
Conventional gel electrophoresis nucleic acid recovery devices are cumbersome to operate and require multiple manual operations, including placement of chips and gels, resulting in a complicated process.
A gel electrophoresis nucleic acid recovery chip was designed, which includes a chip housing, a cover, a mobile base, electrodes and a gel block. The chip recovers nucleic acid fragments through an automated electrophoresis process, reduces manual operation steps, and utilizes the transmission structure and electrodes on the instrument end to achieve automated electrophoresis and nucleic acid extraction.
The nucleic acid recovery process has been simplified. Experimenters only need to add the sample to the chip, and the instrument will automatically recover the nucleic acid fragments without further post-processing, which improves the simplicity and efficiency of the operation.
Smart Images
Figure CN223332937U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of gel electrophoresis nucleic acid recovery equipment, in particular to a gel electrophoresis nucleic acid recovery chip. Background Art
[0002] After DNA molecules are extracted, their quantity and quality need to be tested using electrophoresis. Since the introduction of agarose and polyacrylamide gels into nucleic acid research, gel electrophoresis, which separates DNA by relative molecular mass, has become an important experimental method for analyzing and identifying DNA molecules.
[0003] Because the recovery process requires manual operation, not only adding samples and placing chips in the instrument, but also manually inserting gel, which makes the operation of conventional gel electrophoresis nucleic acid recovery devices more troublesome.
[0004] To this end, we proposed a gel electrophoresis nucleic acid recovery chip to solve the above problems. Utility Model Content
[0005] The purpose of this utility model is to solve the problem that conventional gel electrophoresis nucleic acid recovery device is more troublesome to operate.
[0006] , and a gel electrophoresis nucleic acid recovery chip is proposed.
[0007] In order to achieve the above purpose, the present invention adopts the following technical solutions:
[0008] A gel electrophoresis nucleic acid recovery chip includes a chip housing, a cover plate covering the chip housing, a movable base sealed below the cover plate, inserted into the interior of the chip housing, the lower portion of the movable base being snap-fitted to the chip housing, a negative electrode fixedly embedded in the chip housing, a positive electrode fixedly connected to the instrument end, the cover plate sleeved over the positive electrode, a gel block disposed within the chip housing, a dam assembly mounted within the movable base, and a shell plate fixedly connected to the lower end of the chip housing. The chip housing is encapsulated by the gel block.
[0009] Recovery of front-end nucleic acid fragments: The experimenter adds the sample to be separated and recovered into the chip shell to avoid puncturing the gel block; the target chip is placed horizontally into the instrument to avoid spilling the buffer solution, the transmission structure at the instrument end cooperates with the mobile base, and the electrodes at the target instrument end move the chip shell and the mobile base respectively, and start electrophoresis; the instrument end monitors in real time, and stops electrophoresis when all the target recovered nucleic acid bands pass through the end of the gel block of the chip shell; the instrument moves the target base through the mobile base to disconnect the elution pool containing the target recovered nucleic acid fragments from the chip shell; aspirates the buffer solution in the elution pool; and completes the recovery of the target nucleic acid fragments in the middle or back end of the nucleic acid fragment recovery.
[0010] Return of intermediate or late nucleic acid fragments: The experimenter adds the sample to be separated and recovered into the chip housing to avoid puncturing the gel block; the chip is placed horizontally into the instrument to prevent the buffer from spilling out, and the transmission structure at the instrument end cooperates with the mobile base; the instrument end monitors in real time, and stops electrophoresis when the target recovered nucleic acid band moves to the gel block of the chip housing; the instrument connects the chip housing with the mobile base of the next mobile base elution pool through the mobile base; continues electrophoresis, and the instrument monitors in real time, stops electrophoresis when all the target recovered nucleic acid bands pass through the gel block of the chip housing, and spin-cuts the mobile base to disconnect the elution pool containing the target recovered nucleic acid fragments from the chip housing; aspirates the buffer in the elution pool to complete the recovery of the target nucleic acid fragments.
[0011] Manual operation only requires adding the sample, placing the chip in the instrument, setting the nucleic acid recovery fragment range, and directly extracting the target nucleic acid fragment after the instrument recovery is completed. No further post-processing is required, which improves the insight of the recovery process.
[0012] Preferably, a buffer pool is provided at the bottom of the chip housing, a sample loading hole and a gel channel are provided on the chip housing, the outside of the chip housing is wrapped with a silicone sealing layer, and a chip housing communication port is provided in the chip housing.
[0013] Preferably, a gel loading hole is provided on the gel block.
[0014] Preferably, the cover plate is provided with a first waste liquid pool and a liquid suction hole.
[0015] Preferably, the barrier dam group includes a first barrier dam and a second barrier dam, wherein a semipermeable membrane is sandwiched between the first barrier dam and the second barrier dam, through which biological macromolecules such as nucleic acids cannot pass, but small molecules such as water molecules and salt particles can pass.
[0016] Preferably, the movable base is provided with a dam groove, a movable base connecting port, a transmission slot, a second waste liquid pool and an elution pool.
[0017] In summary, the technical effects and advantages of the present invention are as follows: manual operation only requires adding the sample, placing the chip in the instrument, setting the nucleic acid recovery fragment range, and directly extracting the target nucleic acid fragment after the instrument recovery is completed, without the need for further post-processing, thereby improving the simplicity of the recovery process. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 This is a schematic diagram of the overall split structure of the utility model;
[0019] Figure 2 This is a top structural diagram of the utility model;
[0020] Figure 3 This is a schematic diagram of the bottom structure of the utility model;
[0021] Figure 4 This is a detailed structural diagram of the mobile base of the utility model.
[0022] In the figure: 1. Negative electrode at the instrument end; 2. Positive electrode at the instrument end; 3. Chip shell; 4. Gel block; 5. Cover plate; 6. Dam group; 7. Mobile base; 8. Shell plate; 301. Buffer tank; 302. Sample loading hole; 303. Gel channel; 304. Silicone sealing layer; 305. Chip shell connecting port; 401. Gel loading hole; 501. First waste liquid tank; 502. Liquid aspiration hole; 601. First dam; 602. Second dam; 603. Semipermeable membrane; 701. Transmission slot; 702. Dam slot; 703. Mobile base connecting port; 704. Second waste liquid tank; 705. Elution tank. DETAILED DESCRIPTION
[0023] The technical solutions in the embodiments of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments.
[0024] Reference Figure 1-4 The gel electrophoresis nucleic acid recovery chip includes a chip shell 3, which is covered with a cover plate 5. The lower part of the cover plate 5 is sealed with a movable base 7, which is inserted into the interior of the chip shell 3. The lower part of the movable base 7 is buckled with the chip shell 3. The chip shell 3 is fixedly embedded with an instrument-end negative electrode 1, and the movable base 7 is fixedly connected with an instrument-end positive electrode 2. The cover plate 5 is sleeved on the outside of the instrument-end positive electrode 2. A gel block 4 is provided in the chip shell 3, a dam group 6 is installed in the movable base 7, and the lower end of the chip shell 3 is fixedly connected with a shell plate 8.
[0025] Reference Figure 1-4 A buffer pool 301 is provided at the bottom of the chip shell 3 , a sample loading hole 302 and a gel channel 303 are provided on the chip shell 3 , a silicone sealing layer 304 is wrapped around the outside of the chip shell 3 , and a chip shell connecting port 305 is provided inside the chip shell 3 .
[0026] Reference Figure 1-4 A gel loading hole 401 is provided on the gel block 4 .
[0027] Reference Figure 1-4 The cover plate 5 is provided with a first waste liquid pool 501 and a liquid suction hole 502 .
[0028] Reference Figure 1-4 The dam group 6 includes a first dam 601 and a second dam 602, with a semipermeable membrane 603 sandwiched between the first dam 601 and the second dam 602. The semipermeable membrane 603: biological macromolecules such as nucleic acids cannot pass through, but small molecules such as water molecules and salt particles can pass through.
[0029] Reference Figure 1-4 The movable base 7 is provided with a dam groove 702, a movable base communication port 703, a transmission card slot 701, a second waste liquid pool 704 and an elution pool 705. The second waste liquid pool 704 is connected to the first waste liquid pool 501.
[0030] Working principle:
[0031] Recovery of nucleic acid fragments in the front section: 1. The experimenter adds the sample to be separated and recovered into the sample well 302, avoiding puncturing the wall of the gel sample well 401.
[0032] 2. Place the chip horizontally into the instrument to prevent the buffer from spilling out. The transmission structure at the instrument end is aligned with the transmission slot 701.
[0033] 3. The electrodes at the instrument end move to the buffer pool 301 and the second waste liquid pool 704 respectively, and electrophoresis begins.
[0034] 4. The instrument monitors the target nucleic acid strips in real time and stops electrophoresis when all the target nucleic acid strips have passed through the gel blocks 4 in the chip shell communication port 305.
[0035] 5. The instrument rotates and cuts the mobile base 7 through the transmission slot 701, disconnecting the elution pool containing the target recovered nucleic acid fragment from the chip shell connection port 305.
[0036] 6. Aspirate the buffer solution in the elution pool to complete the recovery of target nucleic acid fragments.
[0037] Recovery of intermediate or late nucleic acid fragments:
[0038] 1. The experimenter adds the sample to be separated and recovered to the sample well 302, avoiding puncturing the wall of the gel sample well 401.
[0039] 2. Place the chip horizontally into the instrument to prevent the buffer from spilling out. The transmission structure at the instrument end is aligned with the transmission slot 701.
[0040] 3. The electrodes at the instrument end move to the buffer pool 301 and the second waste liquid pool 704 respectively, and electrophoresis begins.
[0041] 4. The instrument monitors the target nucleic acid band in real time and stops electrophoresis when the target nucleic acid band moves to the gel block 4 at the chip shell connecting port 305.
[0042] 5. The instrument peels and cuts the movable base 7 through the transmission slot 701 , so that the chip housing connecting port 305 is connected with the movable base connecting port 703 of the next elution pool 705 .
[0043] 6. Continue electrophoresis and monitor the instrument in real time. Stop electrophoresis when all target recovered nucleic acid bands have passed through the gel block 4 of the chip housing connection port 305. Rotate and move the base 7 to disconnect the elution pool containing the target recovered nucleic acid fragments from the chip housing connection port 305.
[0044] 7. Aspirate the buffer solution in the elution pool to complete the recovery of target nucleic acid fragments.
[0045] The above is only a preferred specific implementation method of the utility model, but the scope of protection of the utility model is not limited to this. Any technician familiar with this technical field can make equivalent replacements or changes within the technical scope disclosed by the utility model based on the technical solution and utility model concept of the utility model, which should be covered by the scope of protection of the utility model.
[0046] The description briefly mentions the application direction of the utility model for the existing technology that is known to and has not been changed by those skilled in the art, and combines it with the utility model to form a complete technology; it avoids over-popularizing the technology familiar to those skilled in the art, and is used to assist those skilled in the art to quickly understand the main content of the utility model.
Claims
1. A gel electrophoresis nucleic acid recovery chip, characterized by: The invention comprises a chip shell (3), wherein the chip shell (3) is covered with a cover plate (5), the lower part of the cover plate (5) is sealed with a movable base (7), the movable base (7) is inserted into the interior of the chip shell (3), the lower part of the movable base (7) is buckled with the chip shell (3), the chip shell (3) is fixedly embedded with an instrument end negative electrode (1), the movable base (7) is fixedly connected with an instrument end positive electrode (2), the cover plate (5) is sleeved outside the instrument end positive electrode (2), a gel block (4) is provided in the chip shell (3), a dam group (6) is installed in the movable base (7), and the lower end of the chip shell (3) is fixedly connected with a shell plate (8).
2. The gel electrophoresis nucleic acid recovery chip according to claim 1, characterized in that: A buffer pool (301) is provided at the bottom of the chip housing (3), a sample loading hole (302) and a gel channel (303) are provided on the chip housing (3), a silicone sealing layer (304) is wrapped around the outside of the chip housing (3), and a chip housing communication port (305) is provided inside the chip housing (3).
3. The gel electrophoresis nucleic acid recovery chip according to claim 1, characterized in that: The gel block (4) is provided with a gel loading hole (401).
4. The gel electrophoresis nucleic acid recovery chip according to claim 1, characterized in that: The cover plate (5) is provided with a first waste liquid pool (501) and a liquid suction hole (502).
5. The gel electrophoresis nucleic acid recovery chip according to claim 1, characterized in that: The dam group (6) comprises a first dam (601) and a second dam (602), wherein a semipermeable membrane (603) is sandwiched between the first dam (601) and the second dam (602).
6. The gel electrophoresis nucleic acid recovery chip according to claim 1, characterized in that: The movable base (7) is provided with a dam groove (702), a movable base communication port (703), a transmission slot (701), a second waste liquid pool (704) and an elution pool (705).