Ultracentrifuge for extracting and separating stem cell exosome

Abstract

The utility model discloses an ultra-speed centrifuge for extracting and separating stem cell exosomes, which comprises an ultra-speed centrifuge body, a turntable is fixed at the top of a centrifugal shaft of the ultra-speed centrifuge body, four connecting plates are uniformly connected onto the turntable, a vertical plate is fixed at the other ends of the connecting plates, and a bottom plate block and an upper plate block are fixed on the inner side of the vertical plate. A threaded rod is inserted into the bottom plate block, a bottom tray is fixed to the upper end of the threaded rod, a pressing rod capable of being drawn up and down is inserted into the upper plate block, an upper pressing disc is fixed to the bottom end of the pressing rod, the pressing rod is further sleeved with a pressing spring, and a sliding groove is further formed in the connecting plate. According to the ultra-centrifugal machine for extracting and separating the stem cell exosome, an upper pressing disc is pressed downwards through a pressing spring, a sealing cover of a centrifugal tube is firmly pressed through the upper pressing disc, the sealing cover is prevented from being thrown away, a sliding groove is formed in a connecting plate, after the centrifugal tube is placed, the middle of the centrifugal tube can be limited, deformation of the centrifugal tube is avoided, the service life of the centrifugal tube can be prolonged, and the cost is reduced. Meanwhile, the stability of centrifugal extraction can also be improved.

Description

Technical Field

[0001] The utility model relates to the field of ultracentrifuges, in particular to an ultracentrifuge for extracting and separating stem cell exosomes. Background Art

[0002] An ultracentrifuge is a device that uses centrifugal force to separate liquid-phase heterogeneous systems. Centrifugation is the process of separating, concentrating, and purifying substances using strong centrifugal force according to their sedimentation coefficient, mass, density, and other factors. Generally speaking, a centrifuge speed of 30,000 r / min or higher is called an ultracentrifuge. Ultracentrifugation technology is an indispensable tool in molecular biology, biochemistry research, and industrial production. Traditional ultracentrifuges for stem cell exosome extraction and separation have sealing caps that are easily thrown off when centrifuge tubes are placed and rotated at high speed. In addition, traditional ultracentrifuges place the centrifuge tubes in the placement hole, with only the upper part of the tube supported, while the lower part is unsupported. This can easily cause the tube to deform, thereby affecting the stability of centrifugal extraction.

[0003] To solve the above problems, we proposed an ultracentrifuge for extracting and separating stem cell exosomes, which improves the shortcomings of existing technologies. Utility Model Content

[0004] The technical problem to be solved by the present invention is to overcome the defects of the prior art and provide an ultracentrifuge for extracting and separating stem cell exosomes. In order to solve the above technical problems, the present invention provides the following technical solutions:

[0005] The utility model discloses an ultracentrifuge for extracting and separating exosomes from stem cells, comprising an ultracentrifuge body, a turntable fixed to the top of the centrifugal shaft of the ultracentrifuge body, four connecting plates evenly connected to the turntable, a vertical plate fixed to the other end of the connecting plate, a bottom plate and an upper plate fixed to the inner side of the vertical plate, a threaded rod inserted into the bottom plate, a bottom tray fixed to the upper end of the threaded rod, a pressure rod that can be pulled up and down inserted into the upper plate, an upper pressure plate fixed to the bottom end of the pressure rod, a compression spring sleeved on the pressure rod, and a slide groove formed on the connecting plate.

[0006] Preferably, both ends of the compression spring are fixed on the upper plate and the upper pressure plate respectively.

[0007] Preferably, a bottom groove is provided on the upper surface of the bottom tray.

[0008] Preferably, a limiting groove is provided at the bottom of the upper pressure plate.

[0009] Preferably, a rubber pad is adhered to the inner wall of the chute.

[0010] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0011] A bottom plate and an upper plate are arranged on the vertical plate, a bottom tray which can move up and down is arranged on the bottom plate, a pressure rod, an upper pressure plate and a compression spring combination are arranged on the upper plate, the compression spring is used to press the upper pressure plate downward, and the upper pressure plate is used to firmly press the sealing cover of the centrifuge tube to prevent the sealing cover from being thrown off, and a sliding groove is also provided on the connecting plate, and after the centrifuge tube is placed, the middle part thereof can be limited to prevent the centrifuge tube from being deformed, thereby improving the service life of the centrifuge tube and increasing the stability of centrifugal extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0013] Figure 1 It is a schematic structural diagram of the utility model as a whole;

[0014] Figure 2 This is a schematic diagram of the three-dimensional structure of the connecting plate in the utility model;

[0015] Figure 3 This is a schematic structural diagram of a cutaway bottom tray of the utility model;

[0016] Figure 4 This is a schematic diagram of the three-dimensional structure of the upper pressure plate in the utility model;

[0017] Figure 5 This utility model Figure 1 Schematic diagram of the structure enlarged at point A in the middle.

[0018] In the figure: 1. Ultracentrifuge body; 2. Turntable; 3. Connecting plate; 4. Vertical plate; 5. Bottom plate; 6. Threaded rod; 7. Bottom tray; 8. Upper plate; 9. Pressure rod; 10. Upper pressure plate; 11. Compression spring; 12. Bottom groove; 13. Slide groove; 14. Limit groove; 15. Rubber pad. DETAILED DESCRIPTION

[0019] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention and are not used to limit the present invention. Example

[0020] like Figure 1-5As shown, an ultracentrifuge for extracting and separating exosomes of stem cells comprises an ultracentrifuge body 1, a turntable 2 is fixed to the top of the centrifugal shaft of the ultracentrifuge body 1, four connecting plates 3 are evenly connected to the turntable 2, a vertical plate 4 is fixed to the other end of the connecting plate 3, a bottom plate 5 and an upper plate 8 are fixed to the inner side of the vertical plate 4, a threaded rod 6 is inserted into the bottom plate 5, a bottom tray 7 is fixed to the upper end of the threaded rod 6, a pressure rod 9 that can be pulled up and down is inserted into the upper plate 8, an upper pressure plate 10 is fixed to the bottom end of the pressure rod 9, a compression spring 11 is also sleeved on the pressure rod 9, and a slide groove 13 is also provided on the connecting plate 3. The overall structure of the ultracentrifuge for extracting and separating exosomes of stem cells can effectively prevent the sealing cover from being thrown off, and the connecting plate 3 can limit the middle part of the centrifuge tube to prevent deformation of the centrifuge tube, thereby improving the service life of the centrifuge tube and increasing the stability of centrifugal extraction.

[0021] In this embodiment, both ends of the compression spring 11 are fixed to the upper plate 8 and the upper pressure plate 10 respectively, and are used to press the upper pressure plate 10 downward to tightly press the sealing cover onto the centrifuge tube.

[0022] In this embodiment, a bottom groove 12 is provided on the upper surface of the bottom tray 7 for limiting and fixing the centrifuge tube.

[0023] In this embodiment, a limiting groove 14 is provided at the bottom of the upper pressing plate 10 for limiting and fixing the sealing cover of the centrifuge tube.

[0024] In this embodiment, a rubber pad 15 is adhered to the inner wall of the slide groove 13 to improve the protection effect.

[0025] The principle and advantages of the present invention are as follows: when placing a centrifuge tube, first pinch the bottom of the centrifuge tube with your hands, move the middle part along the slide groove 13 to its bottom, then tilt the centrifuge tube, place its bottom part into the bottom groove 12, then lift the pressure rod 9, compress the compression spring 11, then straighten the centrifuge tube, release the pressure rod 9, at this time the upper pressure plate 10 is firmly pressed on the sealing cover of the centrifuge tube, which can effectively prevent the sealing cover from being thrown off, the connecting plate 3 can limit the middle part of the centrifuge tube, avoid deformation of the centrifuge tube, prolong the service life of the centrifuge tube, and at the same time increase the stability of centrifugal extraction.

[0026] Finally, it should be noted that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. An ultracentrifuge for extracting and separating stem cell exosomes, comprising an ultracentrifuge body (1), characterized in that: A turntable (2) is fixed on the top of the centrifugal shaft of the ultracentrifuge body (1), and four connecting plates (3) are evenly connected to the turntable (2). A vertical plate (4) is fixed to the other end of the connecting plate (3), and a bottom plate (5) and an upper plate (8) are fixed on the inner side of the vertical plate (4). A threaded rod (6) is inserted into the bottom plate (5), and a bottom tray (7) is fixed to the upper end of the threaded rod (6). A pressure rod (9) that can be pulled up and down is inserted into the upper plate (8), and an upper pressure plate (10) is fixed to the bottom end of the pressure rod (9). A compression spring (11) is also sleeved on the pressure rod (9), and a slide groove (13) is also provided on the connecting plate (3).

2. The ultracentrifuge for extracting and separating stem cell exosomes according to claim 1, wherein: The two ends of the compression spring (11) are respectively fixed on the upper plate (8) and the upper pressure plate (10).

3. The ultracentrifuge for extracting and separating stem cell exosomes according to claim 1, wherein: A bottom groove (12) is provided on the upper surface of the bottom tray (7).

4. The ultracentrifuge for extracting and separating stem cell exosomes according to claim 1, wherein: A limiting groove (14) is provided at the bottom of the upper pressure plate (10).

5. The ultracentrifuge for extracting and separating stem cell exosomes according to claim 1, wherein: A rubber pad (15) is adhered to the inner wall of the chute (13).

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