Ventilation disc and cell culture bag

Abstract

The utility model relates to a ventilation disc and a cell culture bag, the ventilation disc comprises a base, a side wall, a protruding seat and an annular cover plate, the protruding seat is arranged on the base and protrudes out of the base, the side wall is arranged around the base and extends upwards, an annular cavity is formed between the protruding seat and the side wall, and the annular cover plate is arranged in the annular cavity. The annular cover plate is used for sealing an upper opening of the annular cavity, a plurality of air holes are further formed in the annular cover plate, and the air holes are communicated with the annular cavity. The cell culture bag comprises a bag body, and a ventilation disc is arranged below the bag body.

Description

Technical Field

[0001] The utility model relates to the field of cell culture, in particular to a ventilation tray and a cell culture bag using the ventilation tray. Background Art

[0002] A bioreactor is an engineered device that provides an appropriate environment for biochemical reactions. It typically uses enzymes or organisms (microorganisms) to simulate biological functions, allowing biochemical reactions to occur outside cells. Both aerobic and anaerobic reactions can occur during the simulation. Based on their origin, they can be categorized as natural bioreactors and engineered bioreactors. Bioreactors are typically cylindrical, with volumes ranging from a few liters to several cubic meters, and are often made of stainless steel, borosilicate glass, or plastic bags.

[0003] A cell culture bag generally includes a bag body, an agitator is provided inside the bag body, and a vent plate is provided at the bottom of the bag body, and the vent plate is used to provide oxygen or air to the culture bag during culture. The vent plate in the prior art is generally designed with 6, 8 or 10 ventilation interfaces at the bottom of the vent plate, and the gas enters the vent plate from these ventilation interfaces and diffuses into the culture medium. Due to the large number of ventilation interfaces, this type of vent plate has problems such as complex ventilation pipelines, difficult installation, and high cost. At the same time, when the operator connects the external gas pipeline at the interface or the bag body is hit by an external force, the rotational pressure generated can easily cause the interface connection to loosen, which can easily lead to the risk of non-sealing of the culture bag, thereby causing contamination of the culture. Utility Model Content

[0004] In order to solve the above problems, the utility model provides a vent plate, including a base, a side wall, a protrusion, and an annular cover plate. The protrusion is arranged on the base and protrudes from the base. The side wall is arranged around the base and extends upward. An annular cavity is formed between the protrusion and the side wall. The annular cover plate is used to close the upper opening of the annular cavity. A plurality of air holes are also provided on the annular cover plate, and the air holes are connected to the annular cavity.

[0005] Furthermore, a first countersunk hole is provided in the annular cavity, and a second countersunk hole is provided on the protruding seat, and the first countersunk hole and the second countersunk hole pass through the base.

[0006] Furthermore, a first fastener is provided in the first countersunk hole, and a second fastener is provided in the second countersunk hole; the first fastener and the second fastener respectively have an integrally formed head and a stem, and the first fastener and the second fastener have a hollow channel;

[0007] The head of the first fastener is matched with the countersunk head of the first countersunk hole, the head of the second fastener is matched with the countersunk head of the second countersunk hole, and the stems of the first fastener and the second fastener pass through the base.

[0008] Furthermore, a thread is provided on the lower portion of the rod, which cooperates with a nut to fixedly connect the first fastener and the second fastener to the base; a pipeline interface is also provided on the outer periphery of the end of the rod.

[0009] Furthermore, at least one first sealing ring is provided between the lower portion of the head of the first fastener and the first countersunk hole, and / or at least one second sealing ring is provided between the stem of the first fastener and the first countersunk hole.

[0010] Furthermore, at least one third sealing ring is provided between the lower portion of the head of the second fastener and the second countersunk hole, and / or at least one fourth sealing ring is provided between the stem of the second fastener and the second countersunk hole.

[0011] Furthermore, the countersink shape of the first countersink hole is not circular and / or the countersink shape of the second countersink hole is not circular.

[0012] The utility model also provides a cell culture bag, comprising a bag body, wherein the above-mentioned vent disc is arranged below the bag body, and the air holes and the hollow channel of the second fastener are connected to the bag body.

[0013] In this invention, the vent disc's external interface is achieved through the ends of the first and second fasteners. The entire vent disc has only two external interfaces: one for supplying gas and one for draining liquid. Compared to existing vent disc structures, this greatly simplifies the system structure and the design of the external vent piping. Furthermore, the dual-seal design significantly enhances the seal between the base and the external fluid. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a diagram of the structure of the cell culture bag;

[0015] Figure 2 This is an exploded view of the vent disc structure;

[0016] Figure 3 This is a structural diagram of the vent disc after assembly;

[0017] Figure 4 It is a cross-sectional view of the vent disc. DETAILED DESCRIPTION

[0018] See also Figure 1, is a schematic diagram of the structure of a cell culture bag using a stirring mechanism. The cell culture bag includes a bag body 1, a vent plate 2, a stirring mechanism 3, multiple pipe interfaces 4, and a drive device interface 5. The stirring mechanism 3 is located within the bag body 1. The multiple pipe interfaces 4 are used to connect the liquid inside the culture bag to the outside. The drive device interface 5 is used to connect an external drive device to drive the stirring mechanism 3 to rotate.

[0019] See also Figure 2-4 The vent plate 2 includes a base 21 , a side wall 22 , a protruding seat 23 , and an annular cover plate 25 .

[0020] The protrusion 23 is arranged on the base 21 and protrudes from the base 21. The side wall 22 is arranged around the base 21 and extends upward. An annular cavity 24 is formed between the protrusion 23 and the side wall 22. The annular cover plate 25 has the same shape as the annular cavity 24, thereby closing the upper opening of the annular cavity 24. A plurality of air holes 26 are also provided on the annular cover plate 25, thereby communicating with the annular cavity 24.

[0021] A first countersunk hole 28 is provided in the annular cavity 24 , and a second countersunk hole 27 is provided on the protruding seat 23 .

[0022] A first fastener is disposed within the first countersunk hole 28. The first fastener comprises an integrally formed head 281 and a stem 282. The head 281 has the same shape as the countersunk head of the first countersunk hole 28. The stem 282 extends along the head 281 and passes through the first countersunk hole 28. The lower portion of the stem 282 is threaded, allowing a nut to securely connect the first fastener to the base 21. The first fastener has a hollow passageway, and a pipe interface (e.g., a snap-fit) is disposed at the outer periphery of the distal end of the stem 282 to facilitate connection to an external air intake pipe. A first sealing ring 283 is disposed between the lower portion of the head 281 and the first countersunk hole 28. A second sealing ring 284 is disposed between the stem 282 and the first countersunk hole 28. An annular groove is provided within the base 21 at the location of the second sealing ring 284 to accommodate the second sealing ring 284.

[0023] In the present invention, the shape of the head 281 is the same as the countersunk shape of the first countersunk hole 28, and is not circular, so as to facilitate the positioning and installation of the first fastener and prevent it from rotating at will. At the same time, the double sealing ring design is adopted, so that the sealing between the base and the external fluid is significantly enhanced. In terms of pipeline design, due to the use of the annular cavity 24, the entire structure only requires two interfaces, one of which leads to the center of the vent disk for discharging the liquid in the cell culture bag, and the other interface is directly connected to the annular cavity 24, and is connected to the interior of the cell culture bag through the air holes on the annular cover plate 25. This not only makes the ventilation more uniform, but also greatly simplifies the structure of the system and the design of the external ventilation pipeline.

[0024] See also Figure 4 The second countersunk hole 27 is provided with a second fastener. The structure and installation method of the second fastener are similar to those of the first fastener and will not be described in detail. The only difference is that the base 21 is changed into a protrusion 23. The hollow channel of the second fastener is used to drain the liquid in the culture bag.

[0025] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments can still be modified, or some of the technical features thereof can be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A ventilated disc, characterized in that: It includes a base (21), a side wall (22), a protruding seat (23), and an annular cover plate (25). The protruding seat (23) is arranged on the base (21) and protrudes from the base (21); the side wall (22) is arranged around the base (21) and extends upward; an annular cavity (24) is formed between the protruding seat (23) and the side wall (22); the annular cover plate (25) is used to close the upper opening of the annular cavity (24); a plurality of air holes (26) are further provided on the annular cover plate (25); and the air holes (26) are communicated with the annular cavity (24).

2. The vent plate according to claim 1, characterized in that A first countersunk hole (28) is provided in the annular cavity (24), and a second countersunk hole (27) is provided on the protruding seat (23). The first countersunk hole (28) and the second countersunk hole (27) pass through the base (21).

3. The vent plate according to claim 2, characterized in that A first fastener is provided in the first countersunk hole (28), and a second fastener is provided in the second countersunk hole (27); the first fastener and the second fastener respectively have an integrally formed head (281) and a rod (282), and the first fastener and the second fastener have a hollow channel; The head of the first fastener cooperates with the countersunk head of the first countersunk hole (28), the head of the second fastener cooperates with the countersunk head of the second countersunk hole (27), and the stems of the first fastener and the second fastener pass through the base (21).

4. The vent disc according to claim 3, characterized in that The lower portion of the rod (282) is provided with a thread, which cooperates with a nut to fixedly connect the first fastener and the second fastener to the base (21); a pipeline interface is also provided on the outer periphery of the end of the rod (282).

5. The vent plate according to claim 3, characterized in that At least one first sealing ring (283) is provided between the lower portion of the head portion (281) of the first fastener and the first countersunk hole (28), and / or at least one second sealing ring (284) is provided between the stem portion (282) of the first fastener and the first countersunk hole (28).

6. The vent plate according to claim 3, characterized in that At least one third sealing ring is provided between the lower portion of the head portion (281) of the second fastener and the second countersunk hole (27), or / and at least one fourth sealing ring is provided between the stem portion of the second fastener and the second countersunk hole (27).

7. The vent plate according to claim 3, wherein: The countersunk shape of the first countersunk hole (28) is not circular and / or the countersunk shape of the second countersunk hole (27) is not circular.

8. A cell culture bag, comprising a bag body, characterized in that: A vent plate according to any one of claims 3 to 7 is provided below the bag body, and the air hole (26) and the hollow channel of the second fastener are in communication with the bag body.

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